FastQCFastQC Report
Fri 27 May 2016
SRR2138932_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138932_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10883508
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153400.041.3628961
GTATCAA262900.033.8545531
TCAACGC301050.028.7893664
ATCAACG304850.028.487923
CAACGCA306950.028.2748015
AACGCAG314900.027.5586916
TATCAAC324100.027.2886412
ACGCAGA359150.024.19937
CGCAGAG368000.023.617338
GTACATG306800.021.9757981
TACATGG311400.021.2630582
ACATGGG308150.020.8569853
GCAGAGT430900.020.009599
CGAGACC68750.019.96585786-87
GAGACCG68950.019.62478388-89
AGACCGA71150.019.5462588-89
GAGTACT242350.019.11517112-13
GACCGAG71500.019.05084490-91
ACCGAGG74950.018.94029290-91
AGAGTAC362200.018.77703310-11
Produced by FastQC (version 0.11.5)