FastQCFastQC Report
Fri 27 May 2016
SRR2138931_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138931_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7738510
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103000.050.3916741
GTATCAA191300.039.200681
ATCAACG225000.032.4030573
ATCTCGT57900.032.0160294
TCAACGC230000.031.6406064
CAACGCA235100.030.9321735
TATCAAC239200.030.8792
AACGCAG237850.030.6266926
ACGCAGA249900.029.201987
CGCAGAG287350.025.4112198
GTACATG251200.024.0310271
TACATGG259700.022.8100132
ACATGGG252250.022.7436163
GCAGAGT330950.022.0110579
TATCTCG65100.021.7122594
GAGTACT174850.021.14104812-13
CTATCTC101000.019.95969294
CATGGGG217550.019.8673464
AGAGTAC291350.019.4631510-11
CAGAGTA320450.019.27978910-11
Produced by FastQC (version 0.11.5)