##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138931_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7738510
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81779980900716	33.0	33.0	33.0	33.0	33.0
2	32.85340769734742	33.0	33.0	33.0	33.0	33.0
3	33.51164423125382	33.0	33.0	33.0	33.0	37.0
4	36.67509223351782	37.0	37.0	37.0	37.0	37.0
5	36.67863270836376	37.0	37.0	37.0	37.0	37.0
6	36.623947504106084	37.0	37.0	37.0	37.0	37.0
7	36.66596269824553	37.0	37.0	37.0	37.0	37.0
8	36.662571735385754	37.0	37.0	37.0	37.0	37.0
9	36.669707605210824	37.0	37.0	37.0	37.0	37.0
10-11	36.64801105122304	37.0	37.0	37.0	37.0	37.0
12-13	36.86263124296538	37.0	37.0	37.0	37.0	38.5
14-15	38.76599377657973	40.0	37.0	40.0	37.0	40.0
16-17	38.71676976575594	40.0	37.0	40.0	37.0	40.0
18-19	38.74069071436232	40.0	37.0	40.0	37.0	40.0
20-21	38.74248775227845	40.0	37.0	40.0	37.0	40.0
22-23	38.64313082374376	40.0	37.0	40.0	37.0	40.0
24-25	38.75501457827778	40.0	37.0	40.0	37.0	40.0
26-27	38.54397651102436	40.0	37.0	40.0	37.0	40.0
28-29	38.556002273439745	40.0	37.0	40.0	37.0	40.0
30-31	38.53318919621202	40.0	37.0	40.0	37.0	40.0
32-33	38.49451063556115	40.0	37.0	40.0	37.0	40.0
34-35	37.820991334357046	40.0	37.0	40.0	35.0	40.0
36-37	34.03498741284524	40.0	37.0	40.0	8.5	40.0
38-39	37.48004011523176	40.0	37.0	40.0	33.0	40.0
40-41	37.95520691899914	40.0	37.0	40.0	35.0	40.0
42-43	38.13736127509871	40.0	37.0	40.0	37.0	40.0
44-45	38.12063639546474	40.0	37.0	40.0	37.0	40.0
46-47	38.01328943785657	40.0	37.0	40.0	33.0	40.0
48-49	37.97287851109134	40.0	37.0	40.0	33.0	40.0
50-51	37.9061891789016	40.0	37.0	40.0	33.0	40.0
52-53	37.81637459043585	40.0	37.0	40.0	33.0	40.0
54-55	37.714954306457386	40.0	37.0	40.0	33.0	40.0
56-57	37.60580979207218	40.0	37.0	40.0	33.0	40.0
58-59	37.44449336215935	38.5	37.0	40.0	33.0	40.0
60-61	37.226811193399996	37.0	37.0	40.0	33.0	40.0
62-63	36.989685110521776	37.0	37.0	40.0	33.0	40.0
64-65	36.76649885067016	37.0	37.0	40.0	33.0	40.0
66-67	36.48175244775339	37.0	37.0	40.0	33.0	40.0
68-69	36.20540220171131	37.0	37.0	38.5	33.0	40.0
70-71	35.94291653436654	37.0	37.0	37.0	33.0	40.0
72-73	35.68665886537369	37.0	35.0	37.0	33.0	40.0
74-75	35.19006108788832	37.0	33.0	37.0	33.0	38.5
76-77	33.77841504162333	35.0	33.0	37.0	27.0	37.0
78-79	35.094015930398044	37.0	33.0	37.0	33.0	37.0
80-81	35.14993759908232	37.0	33.0	37.0	33.0	37.0
82-83	35.218494461475984	37.0	33.0	37.0	33.0	37.0
84-85	35.097093831442244	37.0	33.0	37.0	33.0	37.0
86-87	35.035148836776784	37.0	33.0	37.0	33.0	37.0
88-89	34.958310827881704	37.0	33.0	37.0	33.0	37.0
90-91	34.847571055752354	37.0	33.0	37.0	33.0	37.0
92-93	34.83376690924581	37.0	33.0	37.0	33.0	37.0
94-95	34.793524854123376	37.0	33.0	37.0	33.0	37.0
96-97	34.760756733912146	37.0	33.0	37.0	33.0	37.0
98-99	34.72005522899633	37.0	33.0	37.0	33.0	37.0
100	34.642244885420865	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	3.0
19	9.0
20	49.0
21	217.0
22	837.0
23	2616.0
24	6187.0
25	12338.0
26	21871.0
27	35178.0
28	52052.0
29	73036.0
30	99993.0
31	133181.0
32	177271.0
33	239462.0
34	338240.0
35	535820.0
36	1076743.0
37	2517090.0
38	2334848.0
39	81467.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.32081275814956	0.0	23.529709740493267	30.149477501357175
2	15.35797418836772	23.375808253049158	37.704596877229534	23.56162068135359
3	16.965637540041552	24.735339378140583	32.271860734753716	26.027162347064152
4	13.632101630746154	22.199415547733047	36.571458924528734	27.597023896992063
5	17.81271279127487	36.385435729975605	31.459023306383376	14.34282817236615
6	28.627927081569965	36.36260727194253	20.185565438307894	14.82390020817961
7	26.80642656015176	32.5201492276937	22.13127591745698	18.542148294697558
8	25.045544943406416	31.396276544192613	21.65734747386771	21.90083103853326
9	24.558396900695353	20.141021979683426	23.148306327703914	32.15227479191731
10-11	25.555606024648547	25.23995738455836	27.96836393364898	21.236072657144117
12-13	25.763280011268318	23.450993795963306	27.940133178092424	22.845593014675952
14-15	23.180878695051028	25.60018179211384	25.8282489670653	25.39069054576983
16-17	22.483701642822716	27.896242299874263	26.64498075210861	22.975075305194412
18-19	22.612408590284176	27.14006313876961	28.31834552129544	21.92918274965077
20-21	23.336385252574853	26.370740343486293	28.10180530607498	22.19106909786387
22-23	23.41382030542634	26.05326157109267	27.86801475463098	22.66490336885001
24-25	22.989526868000247	26.394101059670955	28.096905216348922	22.51946685597988
26-27	22.870196720906456	26.62755096464149	27.87557421907919	22.626678095372867
28-29	22.90763464616553	26.381717484839474	27.933951425344674	22.776696443650323
30-31	22.990877350089995	26.346323842735377	28.03258179944992	22.630217007724706
32-33	22.83467718913981	26.72686552445411	27.792804424213802	22.645652862192282
34-35	23.101249077263457	26.28205752610778	27.799472105187228	22.817221291441534
36-37	23.108680196651445	26.532481146658505	27.758723110248734	22.600115546441316
38-39	22.921926145607767	26.724148002757236	27.777802797662606	22.576123053972395
40-41	23.195548743682135	26.377344959268235	27.54804512023607	22.879061176813558
42-43	22.86565418870446	26.52568836180873	27.68266102990212	22.92599641958469
44-45	22.97636982906716	26.79353246677845	27.331148992735255	22.89894871141913
46-47	23.20655312258645	26.444131504395614	27.16607079674761	23.18324457627033
48-49	22.9552729743686	26.87106498010847	27.07830760317983	23.095354442343098
50-51	23.019730085595473	27.13333557260167	26.715027682674314	23.131906659128546
52-53	23.224990523264204	26.627577098810317	26.698291759354316	23.449140618571164
54-55	23.06748916180238	26.50128570014304	27.104110850461034	23.327114287593545
56-57	22.995030881775907	26.738046957825752	26.97190094111606	23.29502121928228
58-59	23.04836387230659	26.402042975358864	27.017150318935517	23.53244283339903
60-61	23.055678090994856	26.59120874541219	27.24390460149591	23.10920856209705
62-63	22.775193228864154	27.02784582048122	27.035035827344974	23.161925123309647
64-65	22.87541213707996	26.91591479046036	26.74154565307324	23.467127419386443
66-67	22.731485706513016	27.25474873529586	26.69141385731931	23.322351700871813
68-69	22.76247917866023	27.308568017217212	26.586194147005628	23.34275865711693
70-71	22.875525584233905	26.708666807589676	26.50389776388505	23.91190984429137
72-73	22.941115478177924	26.623131844229103	26.507425013839775	23.928327663753205
74-75	22.914563650585322	26.830448937382677	26.63039579647376	23.624591615558234
76-77	23.401418044308752	26.43483077478288	26.10044610647969	24.063305074428676
78-79	23.20429737720819	26.616704770212042	26.120470573621944	24.058527278957826
80-81	23.271351513555736	26.972020658318414	25.99825984116454	23.75836798696131
82-83	23.266251022668616	26.57247500873286	26.12535857422737	24.035915394371155
84-85	23.222698344012475	26.944237393335367	26.192213442722267	23.640850819929888
86-87	23.48234514258732	26.933195914358315	26.18126591933748	23.40319302371688
88-89	23.39406775417448	26.67912836793327	26.39005930227571	23.536744575616535
90-91	23.30096666813897	26.63006848679813	26.38700714850342	23.68195769655948
92-93	23.303494157186627	26.51715901463978	26.477960432932825	23.70138639524077
94-95	23.074364860396475	26.41746544608991	26.859769163534285	23.64840052997933
96-97	23.045747014820062	26.61463235829093	26.95641778081753	23.383202846071473
98-99	24.330934799560552	28.720622067482914	26.53363606892872	20.41480706402782
100	23.4949530139137	28.73605483470364	20.850413761507788	26.91857838987487
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1304.0
1	1183.0
2	1888.0
3	2872.0
4	3896.0
5	6590.0
6	8922.5
7	10511.5
8	11596.5
9	11502.5
10	10307.5
11	8957.5
12	9476.5
13	11907.0
14	18216.0
15	28008.0
16	37225.0
17	43150.0
18	43831.5
19	39908.0
20	34172.5
21	28497.5
22	24692.5
23	24781.5
24	29141.5
25	36645.0
26	45225.0
27	55368.5
28	67437.0
29	82042.0
30	97586.5
31	111795.0
32	127249.0
33	141432.0
34	153036.0
35	163451.5
36	176762.5
37	191974.0
38	203585.5
39	212932.5
40	224679.5
41	237314.0
42	247816.5
43	258588.0
44	270835.0
45	285521.0
46	297690.5
47	306312.0
48	316676.5
49	322235.5
50	321179.0
51	321398.0
52	319348.0
53	310184.0
54	299027.5
55	285440.5
56	269425.5
57	249028.5
58	223020.0
59	194789.0
60	163461.5
61	134312.0
62	108055.5
63	82775.0
64	61110.5
65	45321.0
66	34120.5
67	25997.0
68	19842.0
69	14382.0
70	10476.0
71	7722.5
72	5399.5
73	3805.0
74	2620.0
75	1736.0
76	1102.0
77	683.0
78	442.0
79	301.5
80	215.0
81	156.5
82	124.5
83	89.5
84	60.0
85	44.0
86	32.5
87	23.5
88	15.5
89	10.5
90	8.0
91	6.5
92	4.5
93	3.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	2.7137006994886614E-4
2	7.882654412800397E-4
3	0.0038508705164172433
4	0.013038685741828855
5	0.007585439574284973
6	0.0
7	0.0
8	0.0
9	0.0
10-11	6.46119214163967E-6
12-13	0.0
14-15	6.46119214163967E-6
16-17	0.0
18-19	0.0
20-21	1.9384098694009273E-5
22-23	1.9385382568586453E-4
24-25	0.005008776021884021
26-27	0.041970131773413265
28-29	0.08542388920971714
30-31	0.08013933019675983
32-33	0.09528741168446249
34-35	1.3350859405931335
36-37	5.7709418066131635
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.34900135453226E-5
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	2.101573090505436E-5
70-71	7.413229734506827E-4
72-73	0.01375426165088425
74-75	0.0309080084442687
76-77	0.0035205517449177362
78-79	3.644759571976931E-5
80-81	2.794837317668123E-4
82-83	0.007073375096182687
84-85	0.04024322730906005
86-87	0.03997626385695683
88-89	0.009746301106736445
90-91	7.735386655622597E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	132.0
20-21	406.0
22-23	826.0
24-25	2340.0
26-27	2901.0
28-29	5155.0
30-31	9785.0
32-33	12867.0
34-35	20011.0
36-37	20273.0
38-39	20432.0
40-41	31963.0
42-43	38529.0
44-45	48460.0
46-47	47395.0
48-49	40973.0
50-51	20916.0
52-53	8863.0
54-55	8885.0
56-57	10695.0
58-59	32032.0
60-61	47891.0
62-63	47467.0
64-65	51175.0
66-67	56800.0
68-69	55343.0
70-71	55653.0
72-73	54188.0
74-75	56033.0
76-77	55955.0
78-79	60463.0
80-81	61570.0
82-83	62003.0
84-85	63384.0
86-87	67665.0
88-89	75216.0
90-91	80067.0
92-93	87031.0
94-95	116003.0
96-97	414196.0
98-99	1440707.0
100-101	4345861.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.95891274209085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.6063539003921	70.92515161074573
2	8.062391080440676	13.054448319480166
3	2.2510404664263435	5.467253661009774
4	0.9147746362844721	2.962366398305299
5	0.43509774370960386	1.7612520133633216
6	0.23394559549346386	1.1363988631171096
7	0.13829434220628015	0.7837311708382162
8	0.08943340281991666	0.5792344844100715
9	0.055349741091193005	0.4032949373369294
>10	0.2081325085227011	2.6194993209278365
>50	0.004737809751930621	0.2514344121415426
>100	4.4877286129078604E-4	0.055934808323973476
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1630145854951406E-4	0.0	0.0	0.0
3	0.0	1.1630145854951406E-4	0.0	0.0	0.0
4	0.0	1.1630145854951406E-4	0.0	0.0	0.0
5	0.0	1.292238428327934E-4	0.0	0.0	0.0
6	0.0	1.4214622711607272E-4	0.0	0.0	0.0
7	0.0	1.4214622711607272E-4	0.0	0.0	0.0
8	0.0	1.8091337996591075E-4	0.0	0.0	1.2922384283279339E-5
9	0.0	3.230596070819835E-4	0.0	0.0	1.2922384283279339E-5
10-11	0.0	3.295207992236232E-4	0.0	1.2922384283279339E-5	1.2922384283279339E-5
12-13	0.0	3.3598199136526284E-4	0.0	3.230596070819835E-5	1.2922384283279339E-5
14-15	0.0	4.7812821848133554E-4	0.0	5.815072927475703E-5	1.9383576424919007E-5
16-17	0.0	5.298177556144529E-4	0.0	1.0337907426623472E-4	2.5844768566558678E-5
18-19	0.0	5.298177556144529E-4	0.0	2.1968053281574875E-4	2.5844768566558678E-5
20-21	0.0	5.298177556144529E-4	0.0	3.4890437564854214E-4	2.5844768566558678E-5
22-23	0.0	5.815072927475703E-4	0.0	5.556625241810116E-4	2.5844768566558678E-5
24-25	0.0	6.525804063056066E-4	0.0	8.399549784131571E-4	2.5844768566558678E-5
26-27	0.0	6.978087512970843E-4	0.0	0.0017186771096761523	2.5844768566558678E-5
28-29	0.0	7.23653519863643E-4	0.0	0.004193313699924145	2.5844768566558678E-5
30-31	0.0	8.141102098465984E-4	0.0	0.005763383390342585	2.5844768566558678E-5
32-33	0.0	8.141102098465984E-4	0.0	0.008567540779814201	2.5844768566558678E-5
34-35	0.0	9.174892841128332E-4	0.0	0.012373182951239969	2.5844768566558678E-5
36-37	0.0	0.0010273295505207076	0.0	0.023899949731925135	2.5844768566558678E-5
38-39	1.2922384283279339E-5	0.0010596355112289057	0.0	0.10259080882495468	2.5844768566558678E-5
40-41	1.2922384283279339E-5	0.00117593696977842	0.0	0.1637201476770076	2.5844768566558678E-5
42-43	1.2922384283279339E-5	0.0013956175025941686	0.0	0.184118131268164	2.5844768566558678E-5
44-45	1.2922384283279339E-5	0.0016152980354099174	0.0	0.20967860738049054	2.5844768566558678E-5
46-47	1.2922384283279339E-5	0.0016152980354099174	0.0	0.23806908565085527	3.230596070819835E-5
48-49	1.2922384283279339E-5	0.0016282204196931967	0.0	0.2679133321530889	3.8767152849838015E-5
50-51	1.2922384283279339E-5	0.0019125128739253421	0.0	0.3208692629459676	3.8767152849838015E-5
52-53	1.2922384283279339E-5	0.0019125128739253421	0.0	0.6281506388180671	5.815072927475702E-5
54-55	1.2922384283279339E-5	0.0019318964503502612	0.0	1.0273230893285659	7.753430569967603E-5
56-57	1.2922384283279339E-5	0.002015891948191577	0.0	1.4664386296586809	7.753430569967603E-5
58-59	1.2922384283279339E-5	0.002028814332474856	0.0	1.9038354928791201	7.753430569967603E-5
60-61	1.2922384283279339E-5	0.0020611202931830547	0.0	2.011653406146661	7.753430569967603E-5
62-63	1.2922384283279339E-5	0.002112809830316172	0.0	2.0166672912485737	7.753430569967603E-5
64-65	1.2922384283279339E-5	0.002364796323840119	0.0	2.0208670661406396	7.753430569967603E-5
66-67	1.2922384283279339E-5	0.0024294082452565157	0.0	2.0245951740063655	7.753430569967603E-5
68-69	1.2922384283279339E-5	0.0024940201666729125	0.0	2.0285881907498986	7.753430569967603E-5
70-71	1.2922384283279339E-5	0.0025457097038060297	0.0	2.0321806135806506	7.753430569967603E-5
72-73	1.2922384283279339E-5	0.002558632088089309	0.0	2.035378903690762	9.045668998295538E-5
74-75	1.2922384283279339E-5	0.002629705201647346	0.0	2.038111987966676	9.045668998295538E-5
76-77	1.2922384283279339E-5	0.002726623083771941	0.0	2.040857994626873	9.045668998295538E-5
78-79	1.2922384283279339E-5	0.0027653902366217785	0.0	2.0424797538544244	9.045668998295538E-5
80-81	1.2922384283279339E-5	0.0027653902366217785	0.0	2.0429191149200556	1.0984026640787438E-4
82-83	1.2922384283279339E-5	0.0027653902366217785	0.0	2.043377859562112	1.292238428327934E-4
84-85	1.2922384283279339E-5	0.0027653902366217785	0.0	2.0435329281735113	1.292238428327934E-4
86-87	1.2922384283279339E-5	0.0028170797737548958	0.0	2.0436492296320607	1.4214622711607272E-4
88	1.2922384283279339E-5	0.002972148385154248	0.0	2.0436492296320607	1.4214622711607272E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10300	0.0	50.391674	1
GTATCAA	19130	0.0	39.20068	1
ATCAACG	22500	0.0	32.403057	3
ATCTCGT	5790	0.0	32.01602	94
TCAACGC	23000	0.0	31.640606	4
CAACGCA	23510	0.0	30.932173	5
TATCAAC	23920	0.0	30.879	2
AACGCAG	23785	0.0	30.626692	6
ACGCAGA	24990	0.0	29.20198	7
CGCAGAG	28735	0.0	25.411219	8
GTACATG	25120	0.0	24.031027	1
TACATGG	25970	0.0	22.810013	2
ACATGGG	25225	0.0	22.743616	3
GCAGAGT	33095	0.0	22.011057	9
TATCTCG	6510	0.0	21.71225	94
GAGTACT	17485	0.0	21.141048	12-13
CTATCTC	10100	0.0	19.959692	94
CATGGGG	21755	0.0	19.867346	4
AGAGTAC	29135	0.0	19.46315	10-11
CAGAGTA	32045	0.0	19.279789	10-11
>>END_MODULE