FastQCFastQC Report
Fri 27 May 2016
SRR2138930_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138930_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9976481
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117500.053.2560461
GTATCAA223000.038.261421
TCAACGC269850.030.7501474
ATCAACG273450.030.3611973
CAACGCA277350.029.8829525
TATCAAC285600.029.3082142
AACGCAG285550.029.0666486
ACGCAGA325600.025.4913437
CGCAGAG335250.024.7321828
GCAGAGT390200.021.205619
GTACATG284200.021.0289971
TACATGG281650.020.9622782
ACATGGG285200.020.0889723
GAGTACT237850.019.7128512-13
GTGGTAT59000.018.9884171
AGAGTAC350650.018.84270310-11
CAGAGTA380150.018.831210-11
CATGGGG214700.018.114214
GTACTTT263750.017.77760114-15
TGGTATC55950.017.7394772
Produced by FastQC (version 0.11.5)