##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138930_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9976481
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7178745691993	33.0	33.0	33.0	33.0	33.0
2	32.712925730024445	33.0	33.0	33.0	33.0	33.0
3	32.713279762673835	33.0	33.0	33.0	33.0	33.0
4	36.64213393480126	37.0	37.0	37.0	37.0	37.0
5	36.65795374140441	37.0	37.0	37.0	37.0	37.0
6	36.58038009594766	37.0	37.0	37.0	37.0	37.0
7	36.6307405386729	37.0	37.0	37.0	37.0	37.0
8	36.63629720740209	37.0	37.0	37.0	37.0	37.0
9	36.64457547706451	37.0	37.0	37.0	37.0	37.0
10-11	36.60502616102812	37.0	37.0	37.0	37.0	37.0
12-13	36.57838209685359	37.0	37.0	37.0	37.0	37.0
14-15	38.69620024335234	40.0	37.0	40.0	37.0	40.0
16-17	38.646568865314336	40.0	37.0	40.0	37.0	40.0
18-19	38.675067691704115	40.0	37.0	40.0	37.0	40.0
20-21	38.69850218912058	40.0	37.0	40.0	37.0	40.0
22-23	38.54928127863769	40.0	37.0	40.0	37.0	40.0
24-25	38.70291246245067	40.0	37.0	40.0	37.0	40.0
26-27	38.495742447515546	40.0	37.0	40.0	37.0	40.0
28-29	38.484806030362776	40.0	37.0	40.0	37.0	40.0
30-31	38.468833239846006	40.0	37.0	40.0	37.0	40.0
32-33	38.46405244824558	40.0	37.0	40.0	37.0	40.0
34-35	38.37532682504667	40.0	37.0	40.0	37.0	40.0
36-37	33.34688999898399	40.0	37.0	40.0	8.5	40.0
38-39	37.30583737519578	40.0	37.0	40.0	33.0	40.0
40-41	37.88082518093513	40.0	37.0	40.0	35.0	40.0
42-43	38.09571827452304	40.0	37.0	40.0	35.0	40.0
44-45	38.106673601318434	40.0	37.0	40.0	37.0	40.0
46-47	38.007531019601785	40.0	37.0	40.0	33.0	40.0
48-49	37.989497271553255	40.0	37.0	40.0	33.0	40.0
50-51	37.93839291109906	40.0	37.0	40.0	33.0	40.0
52-53	37.86909281025818	40.0	37.0	40.0	33.0	40.0
54-55	37.78430628237831	40.0	37.0	40.0	33.0	40.0
56-57	37.70193663016124	40.0	37.0	40.0	33.0	40.0
58-59	37.558533455141244	40.0	37.0	40.0	33.0	40.0
60-61	37.364642233831646	38.5	37.0	40.0	33.0	40.0
62-63	37.14262249501738	37.0	37.0	40.0	33.0	40.0
64-65	36.935705257113945	37.0	37.0	40.0	33.0	40.0
66-67	36.665766556674576	37.0	37.0	40.0	33.0	40.0
68-69	36.39088269767903	37.0	37.0	40.0	33.0	40.0
70-71	36.12509111139261	37.0	37.0	38.5	33.0	40.0
72-73	35.867048457262214	37.0	37.0	37.0	33.0	40.0
74-75	35.58471628140772	37.0	35.0	37.0	33.0	40.0
76-77	33.73533891396741	35.0	33.0	37.0	27.0	37.0
78-79	35.20039816487531	37.0	33.0	37.0	33.0	37.0
80-81	35.229455769384856	37.0	33.0	37.0	33.0	37.0
82-83	35.30630017969257	37.0	33.0	37.0	33.0	37.0
84-85	35.156025042164714	37.0	33.0	37.0	33.0	37.0
86-87	35.086389560003184	37.0	33.0	37.0	33.0	37.0
88-89	35.00200903250453	37.0	33.0	37.0	33.0	37.0
90-91	34.869305842404245	37.0	33.0	37.0	33.0	37.0
92-93	34.856974358923694	37.0	33.0	37.0	33.0	37.0
94-95	34.81942639294221	37.0	33.0	37.0	33.0	37.0
96-97	34.79669200646175	37.0	33.0	37.0	33.0	37.0
98-99	34.754480677789985	37.0	33.0	37.0	33.0	37.0
100	34.70390772637254	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	9.0
20	46.0
21	285.0
22	1088.0
23	3293.0
24	7741.0
25	16037.0
26	28492.0
27	45619.0
28	67395.0
29	94921.0
30	128849.0
31	172388.0
32	229844.0
33	311620.0
34	440309.0
35	686497.0
36	1337231.0
37	3129967.0
38	3167610.0
39	107237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.25305305548119	20.417610177376172	14.380170723524657	23.949166043617986
2	14.364213193008638	21.198476697344486	41.90494624306908	22.532363866577803
3	17.62178309583601	26.83715232556123	30.85299062590495	24.688073952697803
4	12.251018112849902	18.474621689485378	39.45356590372822	29.820794293936498
5	13.150260551814691	37.87370712759054	35.74158601036794	13.234446310226824
6	29.12490887317883	38.5032858780566	19.162638609746264	13.209166639018308
7	25.796991945356286	32.97376098846878	23.738380296619617	17.490866769555318
8	24.109593352605994	36.614493627562666	21.60861129289977	17.66730172693157
9	25.59444557655149	16.803199444774165	21.410625650467335	36.19172932820701
10-11	23.898120990758166	26.80659142236626	29.294512764570996	20.000774822304578
12-13	24.91124876597269	24.44476664667632	29.151501416180714	21.492483171170278
14-15	22.07400084258167	25.642583792822336	26.893300353100457	25.390115011495535
16-17	21.59106502583426	28.792938111143602	27.455908551321855	22.16008831170029
18-19	21.66701364940203	27.935782166076393	29.147817752572276	21.2493864319493
20-21	22.526665777890937	27.144789585686834	28.987978472916232	21.340566163506
22-23	22.267512731063796	27.17342315249208	28.758671157624605	21.80039295881952
24-25	22.028814774339413	27.303031366395796	28.868348364004593	21.7998054952602
26-27	21.977356435669886	27.41591403894668	28.74301276511389	21.863716760269543
28-29	21.876556434924552	27.4408363939873	28.819938152296853	21.86266901879129
30-31	22.264777448455927	27.352119988619183	28.66273798685176	21.72036457607313
32-33	22.090075494766182	27.44041296970398	28.678265507118045	21.79124602841179
34-35	22.160237244525156	27.48459313490467	28.647763371280632	21.707406249289537
36-37	22.30537074851318	27.38335169401231	28.44244431065952	21.868833246814994
38-39	21.9621922011987	27.486491682197922	28.6881294665316	21.86318665007178
40-41	22.259180586234635	27.349286907099895	28.51935231648014	21.872180190185325
42-43	22.116621991620143	27.43785939186093	28.388980045693373	22.056538570825552
44-45	22.148625732857457	27.610100270565297	28.17140787758423	22.069866118993016
46-47	22.322905200766773	27.508370068932553	27.844199205270026	22.32452552503065
48-49	22.261872377465135	27.586421805649692	27.866652406262123	22.285053410623046
50-51	22.19259749254986	27.78305378875421	27.735572333405567	22.288776385290358
52-53	22.3798521601028	27.706045607193495	27.64798084400008	22.26612138870362
54-55	22.393595213019335	27.595697393763768	27.61544801263612	22.395259380580782
56-57	22.37620973631354	27.707780106354267	27.564949693642742	22.351060463689446
58-59	22.275681293365206	27.81879211021174	27.679887769417167	22.22563882700589
60-61	22.40466559914114	27.821883719193032	27.552570674167416	22.220880007498415
62-63	22.35640073728932	27.92938484130689	27.459734321911167	22.254480099492618
64-65	22.304641821281184	27.85657708419007	27.473051348242223	22.365729746286522
66-67	22.419790750659356	27.847692489506347	27.29294543458455	22.439571325249748
68-69	22.350085878279614	27.820667132803006	27.4222839591224	22.406963029794976
70-71	22.363479803239557	27.56791367207661	27.448439018333232	22.6201675063506
72-73	22.425603946256103	27.590583523922792	27.39778951708982	22.586023012731285
74-75	22.352630138584452	27.77799092162011	27.322017233308443	22.547361706487
76-77	22.46286674478505	27.707151367456056	27.261638938309616	22.568342949449274
78-79	22.410545827932797	27.718889820591418	27.19518808388819	22.67537626758759
80-81	22.53751783582029	27.710531852115466	27.17106835549801	22.58088195656623
82-83	22.498397540597395	27.714518056383085	27.294413265021944	22.492671137997572
84-85	22.625723484206887	27.673559425220628	27.216155322894505	22.48456176767798
86-87	22.440016277267926	27.666741860567406	27.331502386423423	22.561739475741245
88-89	22.46479270165207	27.721088773193443	27.27777278424564	22.536345740908846
90-91	22.491962051854443	27.776909425186936	27.29810210917995	22.43302641377867
92-93	22.589536042386424	27.724667568370176	27.27527838029123	22.410518008952174
94-95	22.367876444490584	27.777092490277106	27.376170286027108	22.478860779205203
96-97	22.509946981869888	27.877306046676047	27.41828640206086	22.1944605693932
98-99	23.63510452323057	29.758451650889604	27.583609197241916	19.02283462863791
100	22.55467362913346	30.762034391390742	21.434432108843293	25.248859870632508
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2060.0
1	1808.5
2	2539.0
3	3754.5
4	5170.5
5	8490.5
6	11159.0
7	12775.0
8	13655.0
9	13175.5
10	11900.0
11	10838.0
12	11982.0
13	16188.5
14	25510.0
15	37824.5
16	48533.5
17	55885.5
18	57898.0
19	55042.5
20	50177.0
21	45400.0
22	43080.0
23	45610.5
24	53073.0
25	66159.0
26	84237.5
27	104396.0
28	125115.5
29	147568.5
30	167953.0
31	187607.0
32	210481.0
33	232229.0
34	255014.5
35	277212.5
36	298847.5
37	318086.5
38	330372.5
39	341830.5
40	356222.5
41	366878.0
42	372056.0
43	379756.5
44	387867.5
45	394698.5
46	398771.5
47	400218.5
48	404449.0
49	403495.0
50	394633.0
51	384071.0
52	371337.5
53	352604.0
54	329940.0
55	305418.0
56	279379.0
57	251790.0
58	223797.0
59	194730.5
60	162723.0
61	132324.0
62	105032.5
63	80372.0
64	59085.5
65	43440.0
66	32717.0
67	25096.5
68	19178.5
69	13990.5
70	9955.0
71	7200.0
72	5146.0
73	3451.5
74	2300.5
75	1513.5
76	1018.5
77	648.0
78	405.0
79	275.5
80	183.5
81	127.0
82	92.0
83	63.0
84	47.5
85	40.0
86	30.5
87	23.0
88	14.5
89	7.5
90	5.0
91	3.0
92	2.5
93	1.0
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.001583724762268379
4	0.014113192818189098
5	0.009241735638047123
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0016592783663035957
26-27	0.03562667139482656
28-29	0.08350073194198222
30-31	0.0808184271637482
32-33	0.036322903064019446
34-35	0.0014064129000425554
36-37	7.398847994917297
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.070204105046098E-5
56-57	3.2325532769227647E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.011476913684749719
74-75	0.03444198101501841
76-77	0.004548048375079481
78-79	0.0
80-81	0.0
82-83	0.003480279190646021
84-85	0.03932248835964975
86-87	0.04326077293031282
88-89	0.012462964884727505
90-91	1.883930537220376E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	122.0
20-21	544.0
22-23	1058.0
24-25	2423.0
26-27	3950.0
28-29	6685.0
30-31	15576.0
32-33	20814.0
34-35	32309.0
36-37	37957.0
38-39	45281.0
40-41	53486.0
42-43	63286.0
44-45	76537.0
46-47	76170.0
48-49	67559.0
50-51	58380.0
52-53	55431.0
54-55	61484.0
56-57	67225.0
58-59	68754.0
60-61	70763.0
62-63	71568.0
64-65	75158.0
66-67	81077.0
68-69	77335.0
70-71	75836.0
72-73	73778.0
74-75	75765.0
76-77	74164.0
78-79	80729.0
80-81	81475.0
82-83	79575.0
84-85	80814.0
86-87	85111.0
88-89	91809.0
90-91	98273.0
92-93	104620.0
94-95	127214.0
96-97	349320.0
98-99	622079.0
100-101	6684987.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.2748033939596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.16913167601798	76.8915497587649
2	6.8457430009707	11.67538776986703
3	1.6409520123114505	4.197955806863439
4	0.6018412236278426	2.0528756807697746
5	0.28278567380086095	1.2057246367998404
6	0.14150843412902722	0.7240262339363929
7	0.08382626892441249	0.5003788021244922
8	0.05744748961259107	0.39190587057521925
9	0.040560923235684596	0.3112942278960443
>10	0.13235969335320152	1.752083386251102
>50	0.0032430056824897776	0.18381759878148704
>100	5.207552025428268E-4	0.058923525885200785
>500	5.9883124486273465E-5	0.03506440477473265
>1k	1.996000673402621E-5	0.019012296710345794
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.004714888947315E-5	0.0	0.0	0.0
3	0.0	2.004714888947315E-5	0.0	0.0	0.0
4	0.0	5.011787222368288E-5	0.0	1.0023574444736576E-5	0.0
5	0.0	6.0141446668419454E-5	0.0	1.0023574444736576E-5	0.0
6	7.016502111315603E-5	6.0141446668419454E-5	0.0	1.0023574444736576E-5	1.0023574444736576E-5
7	7.016502111315603E-5	6.0141446668419454E-5	0.0	1.0023574444736576E-5	1.0023574444736576E-5
8	7.016502111315603E-5	6.0141446668419454E-5	0.0	1.0023574444736576E-5	1.0023574444736576E-5
9	7.016502111315603E-5	1.1025931889210233E-4	0.0	1.0023574444736576E-5	2.004714888947315E-5
10-11	7.016502111315603E-5	1.1025931889210233E-4	0.0	1.5035361667104864E-5	2.004714888947315E-5
12-13	7.016502111315603E-5	1.1025931889210233E-4	0.0	2.004714888947315E-5	2.004714888947315E-5
14-15	7.016502111315603E-5	1.5536540389341692E-4	0.0	2.004714888947315E-5	2.505893611184144E-5
16-17	8.01885955578926E-5	1.603771911157852E-4	0.0	4.00942977789463E-5	3.0070723334209727E-5
18-19	8.01885955578926E-5	1.653889783381535E-4	0.0	5.512965944605117E-5	4.00942977789463E-5
20-21	8.01885955578926E-5	1.704007655605218E-4	0.0	7.016502111315603E-5	4.00942977789463E-5
22-23	8.52003827802609E-5	2.104950633394681E-4	0.0	9.522395722499747E-5	6.0141446668419454E-5
24-25	9.021217000262918E-5	2.556011483407827E-4	0.0	1.5035361667104863E-4	9.021217000262918E-5
26-27	9.021217000262918E-5	2.906836588973607E-4	0.0	2.3054221222894124E-4	1.0023574444736576E-4
28-29	9.021217000262918E-5	2.906836588973607E-4	0.0	5.813673177947214E-4	1.0023574444736576E-4
30-31	9.021217000262918E-5	3.1073080778683384E-4	0.0	0.0022051863778420468	1.0023574444736576E-4
32-33	9.021217000262918E-5	3.1073080778683384E-4	0.0	0.005728472795166953	1.1025931889210233E-4
34-35	1.0023574444736576E-4	3.708722544552533E-4	0.0	0.01059491818808656	1.1025931889210233E-4
36-37	1.0023574444736576E-4	4.4103727556840937E-4	0.0	0.01964119412446132	1.1025931889210233E-4
38-39	1.0023574444736576E-4	4.610844244578825E-4	0.0	0.0424498377734594	1.202828933368389E-4
40-41	1.1025931889210233E-4	5.412730200157751E-4	0.0	0.0658849548252535	1.3030646778157547E-4
42-43	1.1025931889210233E-4	5.663319561276165E-4	0.0	0.08142650700181758	1.4033004222631205E-4
44-45	1.1527110611447062E-4	6.214616155736677E-4	0.0	0.10205502320908544	1.5035361667104863E-4
46-47	1.2028289333683891E-4	6.61555913352614E-4	0.0	0.1221322428218928	1.5536540389341692E-4
48-49	1.2028289333683891E-4	6.665677005749823E-4	0.0	0.14105675137355547	1.603771911157852E-4
50-51	1.5536540389341692E-4	7.617916577999798E-4	0.0	0.15815696937627605	1.603771911157852E-4
52-53	1.603771911157852E-4	7.617916577999798E-4	0.0	0.20067697217084862	1.8042434000525837E-4
54-55	1.603771911157852E-4	7.617916577999798E-4	0.0	0.2527694885601446	2.0047148889473153E-4
56-57	1.603771911157852E-4	8.018859555789261E-4	0.0	0.3069970263061695	2.0047148889473153E-4
58-59	1.603771911157852E-4	8.16921317246031E-4	0.0	0.3406662128660396	2.0047148889473153E-4
60-61	1.704007655605218E-4	8.369684661355041E-4	0.0	0.36536931208509293	2.0047148889473153E-4
62-63	1.9044791444999495E-4	8.52003827802609E-4	0.0	0.3837174651061832	2.0047148889473153E-4
64-65	1.9044791444999495E-4	9.321924233605016E-4	0.0	0.3900924584530357	2.0548327611709982E-4
66-67	1.9044791444999495E-4	9.422159978052381E-4	0.0	0.39552523580208293	2.104950633394681E-4
68-69	1.9044791444999495E-4	9.622631466947113E-4	0.0	0.4014842508094788	2.1550685056183637E-4
70-71	1.9545970167236324E-4	9.823102955841844E-4	0.0	0.40629055475573	2.505893611184144E-4
72-73	2.3054221222894124E-4	9.92333870028921E-4	0.0	0.4103200316825141	2.906836588973607E-4
74-75	2.3054221222894124E-4	0.0010424517422526038	0.0	0.4134173161859377	2.906836588973607E-4
76-77	2.4056578667367784E-4	0.0011126167633657598	0.0	0.41656471856158495	2.9569544611972895E-4
78-79	2.505893611184144E-4	0.001132663912255233	0.0	0.41852933915275337	3.0070723334209726E-4
80-81	2.505893611184144E-4	0.0011376756994776014	0.0	0.41916583612999414	3.057190205644656E-4
82-83	2.505893611184144E-4	0.0011627346355894427	0.0	0.4195667791077836	3.1073080778683384E-4
84-85	2.6562472278551926E-4	0.001167746422811811	0.0	0.4197772741711231	3.1073080778683384E-4
86-87	2.806600844526241E-4	0.001182781784478916	0.0	0.4199476749366836	3.1073080778683384E-4
88	2.906836588973607E-4	0.0012629703800368086	0.0	0.41996772208557304	3.207543822315704E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11750	0.0	53.256046	1
GTATCAA	22300	0.0	38.26142	1
TCAACGC	26985	0.0	30.750147	4
ATCAACG	27345	0.0	30.361197	3
CAACGCA	27735	0.0	29.882952	5
TATCAAC	28560	0.0	29.308214	2
AACGCAG	28555	0.0	29.066648	6
ACGCAGA	32560	0.0	25.491343	7
CGCAGAG	33525	0.0	24.732182	8
GCAGAGT	39020	0.0	21.20561	9
GTACATG	28420	0.0	21.028997	1
TACATGG	28165	0.0	20.962278	2
ACATGGG	28520	0.0	20.088972	3
GAGTACT	23785	0.0	19.71285	12-13
GTGGTAT	5900	0.0	18.988417	1
AGAGTAC	35065	0.0	18.842703	10-11
CAGAGTA	38015	0.0	18.8312	10-11
CATGGGG	21470	0.0	18.11421	4
GTACTTT	26375	0.0	17.777601	14-15
TGGTATC	5595	0.0	17.739477	2
>>END_MODULE