FastQCFastQC Report
Fri 27 May 2016
SRR2138929_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138929_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11027806
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162250.042.396371
GTATCAA276950.035.7042471
TCAACGC328650.029.5689874
ATCAACG332500.029.2015343
CAACGCA335750.028.9136245
AACGCAG343350.028.2820436
TATCAAC345150.028.2546042
ACGCAGA387600.025.0971647
CGCAGAG395800.024.544968
GTACATG322700.021.4087641
GCAGAGT458600.020.9703929
TACATGG324100.020.685862
GAGTACT254850.020.27893312-13
ACATGGG328800.020.0017813
AGAGTAC388350.019.12620210-11
CAGAGTA438600.018.62308710-11
GTACTTT281850.018.26159714-15
CATGGGG254000.017.8308494
AGTACTT289250.016.25627712-13
ACTTTTT331650.015.5015916-17
Produced by FastQC (version 0.11.5)