##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138929_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11027806
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.719259207135124	33.0	33.0	33.0	33.0	33.0
2	32.712092958472425	33.0	33.0	33.0	33.0	33.0
3	32.71204852533677	33.0	33.0	33.0	33.0	33.0
4	36.63973377841431	37.0	37.0	37.0	37.0	37.0
5	36.65718403098495	37.0	37.0	37.0	37.0	37.0
6	36.58379672257564	37.0	37.0	37.0	37.0	37.0
7	36.63076744367828	37.0	37.0	37.0	37.0	37.0
8	36.63478138806577	37.0	37.0	37.0	37.0	37.0
9	36.64334800594062	37.0	37.0	37.0	37.0	37.0
10-11	36.60300457770113	37.0	37.0	37.0	37.0	37.0
12-13	36.57618038438471	37.0	37.0	37.0	37.0	37.0
14-15	38.689020009963905	40.0	37.0	40.0	37.0	40.0
16-17	38.63864371571281	40.0	37.0	40.0	37.0	40.0
18-19	38.663824381749194	40.0	37.0	40.0	37.0	40.0
20-21	38.68746116467864	40.0	37.0	40.0	37.0	40.0
22-23	38.545725303221296	40.0	37.0	40.0	37.0	40.0
24-25	38.69577047628141	40.0	37.0	40.0	37.0	40.0
26-27	38.48912091547392	40.0	37.0	40.0	37.0	40.0
28-29	38.47881913240378	40.0	37.0	40.0	37.0	40.0
30-31	38.46280885406517	40.0	37.0	40.0	37.0	40.0
32-33	38.45772118234964	40.0	37.0	40.0	37.0	40.0
34-35	38.368627484264756	40.0	37.0	40.0	37.0	40.0
36-37	33.425322276314944	40.0	37.0	40.0	8.5	40.0
38-39	37.31807166224456	40.0	37.0	40.0	33.0	40.0
40-41	37.876878428241604	40.0	37.0	40.0	35.0	40.0
42-43	38.08469864854209	40.0	37.0	40.0	35.0	40.0
44-45	38.091694569368585	40.0	37.0	40.0	35.0	40.0
46-47	37.98744750765246	40.0	37.0	40.0	33.0	40.0
48-49	37.96419674034961	40.0	37.0	40.0	33.0	40.0
50-51	37.91793484176236	40.0	37.0	40.0	33.0	40.0
52-53	37.84390384337604	40.0	37.0	40.0	33.0	40.0
54-55	37.75818011511659	40.0	37.0	40.0	33.0	40.0
56-57	37.67746032954906	40.0	37.0	40.0	33.0	40.0
58-59	37.53202159835746	40.0	37.0	40.0	33.0	40.0
60-61	37.33626449880053	37.0	37.0	40.0	33.0	40.0
62-63	37.11288438179452	37.0	37.0	40.0	33.0	40.0
64-65	36.901694663960306	37.0	37.0	40.0	33.0	40.0
66-67	36.63154510778004	37.0	37.0	40.0	33.0	40.0
68-69	36.35326673961272	37.0	37.0	40.0	33.0	40.0
70-71	36.08598912870391	37.0	37.0	38.5	33.0	40.0
72-73	35.82852382566384	37.0	37.0	37.0	33.0	40.0
74-75	35.545159382858984	37.0	33.0	37.0	33.0	40.0
76-77	33.698206063547204	35.0	33.0	37.0	27.0	37.0
78-79	35.17233558870147	37.0	33.0	37.0	33.0	37.0
80-81	35.20545109649136	37.0	33.0	37.0	33.0	37.0
82-83	35.28177193747089	37.0	33.0	37.0	33.0	37.0
84-85	35.13008868537565	37.0	33.0	37.0	33.0	37.0
86-87	35.063081629936676	37.0	33.0	37.0	33.0	37.0
88-89	34.9781713638605	37.0	33.0	37.0	33.0	37.0
90-91	34.84337401582498	37.0	33.0	37.0	33.0	37.0
92-93	34.82764469810556	37.0	33.0	37.0	33.0	37.0
94-95	34.7890343729794	37.0	33.0	37.0	33.0	37.0
96-97	34.76702014324445	37.0	33.0	37.0	33.0	37.0
98-99	34.72561008699759	37.0	33.0	37.0	33.0	37.0
100	34.67140966821296	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	10.0
20	62.0
21	316.0
22	1253.0
23	3653.0
24	8712.0
25	17737.0
26	31246.0
27	50378.0
28	74212.0
29	105407.0
30	143012.0
31	192053.0
32	256861.0
33	349580.0
34	492968.0
35	769422.0
36	1498923.0
37	3452009.0
38	3463579.0
39	116411.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.79815096493355	20.05783380665202	14.20559991715487	23.938415311259558
2	14.538322491345967	21.05642772460814	41.77695907962109	22.628290704424796
3	18.00880810071044	26.44658263973878	30.515373460133034	25.029235799417748
4	12.396270358217565	18.21482533515444	39.133840486257355	30.255063820370644
5	13.331239034632288	37.76430469797026	35.518541163761405	13.385915103636044
6	29.588813949030296	38.04479331609569	18.999790166783857	13.366602568090153
7	26.14412150522053	32.83887112268751	23.387707400728665	17.629299971363295
8	24.28897461562164	36.536333700465896	21.264964218630613	17.90972746528185
9	25.688618388825486	16.829820909072936	21.28426996267435	36.197290739427224
10-11	24.14307070690217	26.659042605573585	29.04098059033683	20.15690609718742
12-13	25.112873766549754	24.32600827399394	28.96472788875684	21.596390070699467
14-15	22.2430146123354	25.528582022570944	26.757366787192304	25.47103657790135
16-17	21.798778469624875	28.66731152143953	27.26966723934026	22.26424276959533
18-19	21.822727022945454	27.85401738115451	28.984124312669262	21.33913128323077
20-21	22.665738792681967	26.989919935048825	28.84777176258741	21.496569509681805
22-23	22.43421687177502	26.931671214343233	28.658579083432727	21.975532830449016
24-25	22.170071236326695	27.08710263862823	28.737253868928114	22.005572256116963
26-27	22.09897572195989	27.16977835146062	28.704207491644386	22.027038434935108
28-29	22.036682925251323	27.25303852925194	28.729504712951147	21.980773832545587
30-31	22.279726676839264	27.085451550842176	28.62962699877952	22.00519477353903
32-33	22.11311902167884	27.216488385314324	28.597275531351173	22.073117061655662
34-35	22.305040996890614	27.332220223447322	28.50779860654243	21.854940173119633
36-37	22.43947299216222	27.265926046376265	28.279578199311516	22.015022762150004
38-39	22.12035624539458	27.405589288068242	28.49310259389769	21.980951872639487
40-41	22.424109125222024	27.257146425399643	28.322713143872114	21.996031305506218
42-43	22.23606578490767	27.344405557149447	28.16750325171468	22.2520254062282
44-45	22.307356266642994	27.455472334752752	27.999249555190854	22.2379218434134
46-47	22.469997999323088	27.41423639720484	27.724523284254126	22.391242319217948
48-49	22.369013919829705	27.53912407562086	27.693532096338064	22.39832990821137
50-51	22.357493397809698	27.683737747933368	27.59942301830991	22.35934583594702
52-53	22.500871784419544	27.615227683043337	27.493660847364797	22.39023968517232
54-55	22.496495227959162	27.474384614913504	27.54842690393381	22.48069325319352
56-57	22.488439640639037	27.60497273251492	27.43563175559609	22.470955871249952
58-59	22.440820295993927	27.662649086387788	27.53597120351187	22.360559414106408
60-61	22.562278623347463	27.651651624793143	27.47422981359207	22.311839938267326
62-63	22.503423896968485	27.750937754264026	27.41425038033843	22.331387968429055
64-65	22.425228174834924	27.749628039839962	27.370738010632596	22.45440577469252
66-67	22.53902577999913	27.70625062778202	27.24705765499207	22.507665937226783
68-69	22.43599410383738	27.6691002007853	27.34758605439897	22.54731964097835
70-71	22.482105149572583	27.45070728683715	27.356683013776458	22.71050454981381
72-73	22.515169628443083	27.494091037200818	27.285352604153502	22.705386730202598
74-75	22.39754374613484	27.615824127148997	27.291855364405066	22.6947767623111
76-77	22.537059056783825	27.54388616197171	27.218814497950806	22.700240283293656
78-79	22.446749739448677	27.576060892652855	27.197909159350242	22.77928020854823
80-81	22.62559751740423	27.549226390280353	27.110535811125917	22.714640281189496
82-83	22.53707226826146	27.541227813429675	27.29395376368982	22.627746154619043
84-85	22.619524103896648	27.587939421378543	27.180865114242525	22.611671360482283
86-87	22.487291260498925	27.613340707368337	27.252297351205346	22.647070680927392
88-89	22.483758440000237	27.57673590417655	27.236776196239166	22.702729459584045
90-91	22.493739290742344	27.601543874005934	27.24348671753366	22.66123011771806
92-93	22.559186094053278	27.554172844318455	27.305304403521767	22.581336658106505
94-95	22.421730580470587	27.634042922830464	27.339699631021556	22.604526865677393
96-97	22.459883341033667	27.696740965780357	27.436056556274497	22.40731913691148
98-99	23.635477595444762	29.631509213239145	27.559716396398727	19.17329679491737
100	22.511488812462375	30.687131500505828	21.376858516243104	25.424521170788694
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3153.0
1	2663.0
2	3112.5
3	4279.5
4	5639.5
5	8853.0
6	11563.5
7	13508.0
8	15075.0
9	15222.5
10	14110.0
11	12961.5
12	13969.5
13	18140.0
14	27515.0
15	40165.5
16	51396.0
17	59145.5
18	61546.5
19	58761.0
20	53757.5
21	48801.5
22	46141.5
23	48620.0
24	56567.0
25	69746.5
26	88063.5
27	108499.5
28	130337.0
29	154348.0
30	177549.0
31	200612.5
32	224061.5
33	247273.0
34	270339.0
35	291517.0
36	315081.0
37	337612.5
38	355184.0
39	371477.5
40	388826.0
41	403909.0
42	412935.5
43	421062.0
44	429685.0
45	439557.5
46	451064.5
47	460425.5
48	463091.0
49	456408.0
50	446427.5
51	432728.5
52	412979.0
53	390999.5
54	368281.5
55	343745.0
56	318459.5
57	290749.0
58	259448.5
59	225606.0
60	188634.0
61	153515.5
62	122576.0
63	95799.0
64	73387.0
65	57012.5
66	44946.5
67	34159.0
68	25245.5
69	18743.0
70	13462.0
71	9094.0
72	6163.0
73	4148.5
74	2803.0
75	1892.0
76	1222.0
77	759.0
78	502.5
79	365.0
80	263.5
81	181.0
82	139.5
83	103.0
84	69.0
85	48.5
86	35.0
87	26.5
88	21.0
89	15.0
90	8.5
91	3.0
92	3.0
93	4.5
94	4.5
95	3.5
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.002085636979830802
4	0.014019107699210522
5	0.009466978291058076
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0014876302861388823
26-27	0.036694970066922045
28-29	0.08445102859437201
30-31	0.0820343724656768
32-33	0.03746957673854443
34-35	0.0012959596222788783
36-37	7.271631163638885
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	4.374583533571794E-5
56-57	7.342003031561998E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.011416527894400563
74-75	0.03452065425250223
76-77	0.004684866505472556
78-79	0.0
80-81	0.0
82-83	0.003238053208148995
84-85	0.0404605446297998
86-87	0.04445497020982668
88-89	0.012379150024274607
90-91	2.2756900660325607E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	240.0
20-21	940.0
22-23	1475.0
24-25	3434.0
26-27	5383.0
28-29	7990.0
30-31	19203.0
32-33	24345.0
34-35	37738.0
36-37	46864.0
38-39	55567.0
40-41	61887.0
42-43	72918.0
44-45	88404.0
46-47	87104.0
48-49	77668.0
50-51	68408.0
52-53	64492.0
54-55	69752.0
56-57	74504.0
58-59	77228.0
60-61	76243.0
62-63	76296.0
64-65	81449.0
66-67	86035.0
68-69	84459.0
70-71	83921.0
72-73	79721.0
74-75	81265.0
76-77	80194.0
78-79	86807.0
80-81	87560.0
82-83	86177.0
84-85	86476.0
86-87	91403.0
88-89	99576.0
90-91	105613.0
92-93	112617.0
94-95	137280.0
96-97	387259.0
98-99	689947.0
100-101	7381964.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.4331331372709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.62950554408971	78.33392118857337
2	6.58773659493267	11.387974283661743
3	1.5888092285169644	4.119772787343944
4	0.553709973642247	1.9143555148502043
5	0.2580824636470674	1.1153437970400926
6	0.12279269838435829	0.6368014588643994
7	0.08146235447819476	0.4928732571202555
8	0.04820375935313901	0.33331215599094655
9	0.031243614097542276	0.2430435111284147
>10	0.0963298472278467	1.2413888302407539
>50	0.001678344379163309	0.09229517170473735
>100	4.1322525959125553E-4	0.0631224236018485
>500	1.61762857478779E-5	0.010108747841724039
>1k	1.617570585084392E-5	0.015686872037575537
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.067986868829575E-6
2	0.0	2.7203960606488726E-5	0.0	0.0	9.067986868829575E-6
3	0.0	2.7203960606488726E-5	0.0	0.0	9.067986868829575E-6
4	0.0	9.974785555712533E-5	0.0	1.813597373765915E-5	9.067986868829575E-6
5	0.0	9.974785555712533E-5	0.0	2.7203960606488726E-5	9.067986868829575E-6
6	0.0	9.974785555712533E-5	0.0	2.7203960606488726E-5	9.067986868829575E-6
7	0.0	9.974785555712533E-5	0.0	2.7203960606488726E-5	9.067986868829575E-6
8	0.0	1.088158424259549E-4	0.0	3.62719474753183E-5	9.067986868829575E-6
9	0.0	1.6322376363893235E-4	0.0	6.347590808180703E-5	9.067986868829575E-6
10-11	0.0	1.6322376363893235E-4	0.0	1.3601980303244363E-4	9.067986868829575E-6
12-13	0.0	1.6322376363893235E-4	0.0	1.6775775707334714E-4	9.067986868829575E-6
14-15	0.0	2.4483564545839853E-4	0.0	2.4483564545839853E-4	2.2669967172073938E-5
16-17	0.0	2.4483564545839853E-4	0.0	3.1284554697462035E-4	3.173795404090351E-5
18-19	0.0	2.493696388928133E-4	0.0	3.7632145505642737E-4	3.62719474753183E-5
20-21	0.0	2.6750561263047247E-4	0.0	4.715353171791379E-4	3.62719474753183E-5
22-23	0.0	3.0377756010579077E-4	0.0	5.803511596050928E-4	4.080594090973309E-5
24-25	0.0	3.309815207122795E-4	0.0	6.755650217278033E-4	4.5339934344147876E-5
26-27	0.0	3.4911749444993865E-4	0.0	7.617108969816843E-4	5.440792121297745E-5
28-29	0.0	3.581854813187682E-4	0.0	9.974785555712533E-4	5.440792121297745E-5
30-31	0.0	4.352633697038196E-4	0.0	0.0016911795510367157	5.894191464739224E-5
32-33	0.0	4.352633697038196E-4	0.0	0.0037541465636954444	7.25438949506366E-5
34-35	0.0	5.032732712200415E-4	0.0	0.006302250873836554	9.067986868829575E-5
36-37	9.067986868829575E-6	5.712831727362632E-4	0.0	0.01055967070875204	9.974785555712533E-5
38-39	9.067986868829575E-6	5.712831727362632E-4	0.0	0.022783317007934307	9.974785555712533E-5
40-41	9.067986868829575E-6	6.347590808180703E-4	0.0	0.03530167288035353	9.974785555712533E-5
42-43	9.067986868829575E-6	6.528950545557294E-4	0.0	0.04332684125926771	9.974785555712533E-5
44-45	9.067986868829575E-6	7.617108969816843E-4	0.0	0.05399079381701129	9.974785555712533E-5
46-47	9.067986868829575E-6	8.387887853667357E-4	0.0	0.06535751535708915	9.974785555712533E-5
48-49	9.067986868829575E-6	8.433227788011505E-4	0.0	0.07651567319918395	1.088158424259549E-4
50-51	9.067986868829575E-6	9.340026474894462E-4	0.0	0.08754687922511513	1.088158424259549E-4
52-53	9.067986868829575E-6	9.476046277926906E-4	0.0	0.11514983125383235	1.1788382929478448E-4
54-55	9.067986868829575E-6	9.566726146615202E-4	0.0	0.14845201302960898	1.2695181616361405E-4
56-57	9.067986868829575E-6	9.793425818335941E-4	0.0	0.1839985215554209	1.3601980303244363E-4
58-59	9.067986868829575E-6	9.793425818335941E-4	0.0	0.2151470564498505	1.450877899012732E-4
60-61	9.067986868829575E-6	9.884105687024236E-4	0.0	0.22815054961975212	1.5415577677010278E-4
62-63	9.067986868829575E-6	0.0010156145293089124	0.0	0.24513942301850433	1.7229175050776193E-4
64-65	9.067986868829575E-6	0.0012241782272919925	0.0	0.25166837356406163	1.7229175050776193E-4
66-67	9.067986868829575E-6	0.0012332462141608222	0.0	0.25690513598081066	1.7229175050776193E-4
68-69	9.067986868829575E-6	0.001260450174767311	0.0	0.26235046209554286	1.7229175050776193E-4
70-71	9.067986868829575E-6	0.0012695181616361405	0.0	0.2671973010769323	1.7229175050776193E-4
72-73	9.067986868829575E-6	0.0012695181616361405	0.0	0.27137310903002826	1.858937308110063E-4
74-75	9.067986868829575E-6	0.0012921881288082144	0.0	0.274365544696742	1.9042772424542108E-4
76-77	9.067986868829575E-6	0.001364732023758851	0.0	0.2773534463700214	1.9042772424542108E-4
78-79	9.067986868829575E-6	0.001405537964668584	0.0	0.27903102394075485	1.9042772424542108E-4
80-81	9.067986868829575E-6	0.001405537964668584	0.0	0.27967485100844175	1.9496171767983587E-4
82-83	9.067986868829575E-6	0.0014146059515374137	0.0	0.28018719226653066	1.9949571111425065E-4
84-85	9.067986868829575E-6	0.0014327419252750729	0.0	0.2804093579448169	1.9949571111425065E-4
86-87	9.067986868829575E-6	0.0014599458858815615	0.0	0.280486435833202	2.0402970454866544E-4
88	9.067986868829575E-6	0.001532489780832198	0.0	0.28050003781350524	2.0856369798308023E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16225	0.0	42.39637	1
GTATCAA	27695	0.0	35.704247	1
TCAACGC	32865	0.0	29.568987	4
ATCAACG	33250	0.0	29.201534	3
CAACGCA	33575	0.0	28.913624	5
AACGCAG	34335	0.0	28.282043	6
TATCAAC	34515	0.0	28.254604	2
ACGCAGA	38760	0.0	25.097164	7
CGCAGAG	39580	0.0	24.54496	8
GTACATG	32270	0.0	21.408764	1
GCAGAGT	45860	0.0	20.970392	9
TACATGG	32410	0.0	20.68586	2
GAGTACT	25485	0.0	20.278933	12-13
ACATGGG	32880	0.0	20.001781	3
AGAGTAC	38835	0.0	19.126202	10-11
CAGAGTA	43860	0.0	18.623087	10-11
GTACTTT	28185	0.0	18.261597	14-15
CATGGGG	25400	0.0	17.830849	4
AGTACTT	28925	0.0	16.256277	12-13
ACTTTTT	33165	0.0	15.50159	16-17
>>END_MODULE