FastQCFastQC Report
Fri 27 May 2016
SRR2138928_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138928_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11480989
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160700.047.1423341
GTATCAA298450.035.760181
TCAACGC322050.032.1323174
ATCAACG326400.031.7570483
CAACGCA329900.031.4285415
AACGCAG340150.030.5288456
TATCAAC346950.030.0113072
ACGCAGA390000.026.5938267
CGCAGAG401700.025.8510998
GCAGAGT467350.022.1649689
GTACATG348750.020.7441481
GAGTACT265450.020.65243312-13
TACATGG346150.020.370072
ACATGGG346600.019.9497973
CAGAGTA462250.019.26812410-11
AGAGTAC429750.018.88446810-11
GTACTTT296150.018.54105414-15
GTGGTAT73650.018.2440741
CATGGGG283650.017.5343764
AGTACTT307700.016.38218712-13
Produced by FastQC (version 0.11.5)