##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138928_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11480989
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71705373117246	33.0	33.0	33.0	33.0	33.0
2	32.711492450693925	33.0	33.0	33.0	33.0	33.0
3	32.71140482758062	33.0	33.0	33.0	33.0	33.0
4	36.640357202676526	37.0	37.0	37.0	37.0	37.0
5	36.65652096696547	37.0	37.0	37.0	37.0	37.0
6	36.58391197831476	37.0	37.0	37.0	37.0	37.0
7	36.62954088711347	37.0	37.0	37.0	37.0	37.0
8	36.6334558808479	37.0	37.0	37.0	37.0	37.0
9	36.64086935367676	37.0	37.0	37.0	37.0	37.0
10-11	36.601843403908845	37.0	37.0	37.0	37.0	37.0
12-13	36.57372282997571	37.0	37.0	37.0	37.0	37.0
14-15	38.68704155190811	40.0	37.0	40.0	37.0	40.0
16-17	38.6364486108296	40.0	37.0	40.0	37.0	40.0
18-19	38.66506343660812	40.0	37.0	40.0	37.0	40.0
20-21	38.68779295403239	40.0	37.0	40.0	37.0	40.0
22-23	38.54705006969003	40.0	37.0	40.0	37.0	40.0
24-25	38.69307033127559	40.0	37.0	40.0	37.0	40.0
26-27	38.48680052878677	40.0	37.0	40.0	37.0	40.0
28-29	38.47882379640687	40.0	37.0	40.0	37.0	40.0
30-31	38.460610489448285	40.0	37.0	40.0	37.0	40.0
32-33	38.459691472987515	40.0	37.0	40.0	37.0	40.0
34-35	38.37455910680106	40.0	37.0	40.0	37.0	40.0
36-37	33.45719031084542	40.0	37.0	40.0	8.5	40.0
38-39	37.328895256924014	40.0	37.0	40.0	33.0	40.0
40-41	37.88813713358289	40.0	37.0	40.0	35.0	40.0
42-43	38.093908987464005	40.0	37.0	40.0	35.0	40.0
44-45	38.09914255830803	40.0	37.0	40.0	35.0	40.0
46-47	37.99441387947846	40.0	37.0	40.0	33.0	40.0
48-49	37.97105518690489	40.0	37.0	40.0	33.0	40.0
50-51	37.927994810861705	40.0	37.0	40.0	33.0	40.0
52-53	37.855152991475286	40.0	37.0	40.0	33.0	40.0
54-55	37.769212823962896	40.0	37.0	40.0	33.0	40.0
56-57	37.68945193775009	40.0	37.0	40.0	33.0	40.0
58-59	37.542645292311875	40.0	37.0	40.0	33.0	40.0
60-61	37.35278644907762	37.0	37.0	40.0	33.0	40.0
62-63	37.12560807730364	37.0	37.0	40.0	33.0	40.0
64-65	36.90967657826705	37.0	37.0	40.0	33.0	40.0
66-67	36.633808814224324	37.0	37.0	40.0	33.0	40.0
68-69	36.35576982256279	37.0	37.0	40.0	33.0	40.0
70-71	36.092294901335734	37.0	37.0	38.5	33.0	40.0
72-73	35.83130917536165	37.0	37.0	37.0	33.0	40.0
74-75	35.54808339945174	37.0	33.0	37.0	33.0	40.0
76-77	33.70534293019947	35.0	33.0	37.0	27.0	37.0
78-79	35.1828290646913	37.0	33.0	37.0	33.0	37.0
80-81	35.21691116403191	37.0	33.0	37.0	33.0	37.0
82-83	35.290059291789845	37.0	33.0	37.0	33.0	37.0
84-85	35.13671536674205	37.0	33.0	37.0	33.0	37.0
86-87	35.06840537111138	37.0	33.0	37.0	33.0	37.0
88-89	34.98302468806557	37.0	33.0	37.0	33.0	37.0
90-91	34.847099535290226	37.0	33.0	37.0	33.0	37.0
92-93	34.82462672416683	37.0	33.0	37.0	33.0	37.0
94-95	34.78425223936718	37.0	33.0	37.0	33.0	37.0
96-97	34.759963526342645	37.0	33.0	37.0	33.0	37.0
98-99	34.71528948750787	37.0	33.0	37.0	33.0	37.0
100	34.65591559353328	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	67.0
21	307.0
22	1273.0
23	3621.0
24	8766.0
25	17707.0
26	31800.0
27	51289.0
28	76645.0
29	108525.0
30	150295.0
31	200431.0
32	267638.0
33	364276.0
34	514575.0
35	802562.0
36	1564059.0
37	3606656.0
38	3591658.0
39	118833.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.684196370190755	20.06538809505	14.426387831222554	23.82402770353669
2	14.469981636599424	21.088061315971995	41.7107184755599	22.73123857186868
3	17.95781588460074	26.315506620768453	30.563489355719874	25.16318813891093
4	12.54922894900016	18.13704677353403	38.9804073791897	30.333316898276113
5	13.411847838272697	37.716396381611474	35.48495167691543	13.386804103200397
6	29.772165098320365	38.10889462571561	18.73298545970212	13.385954816261908
7	26.389886794595828	32.81860125464801	23.257891807055994	17.533620143700162
8	24.48105298245648	36.730145808867164	21.05403114661986	17.7347700620565
9	25.690138715401613	17.02735713796085	21.139206735586978	36.14329741105056
10-11	24.299579069364146	26.73912935549368	28.865705733190755	20.095585841951422
12-13	25.18718988407706	24.34620397249749	28.93978471715285	21.526821426272598
14-15	22.36562111504505	25.536711166607684	26.619588260209987	25.478079458137277
16-17	21.727170019934693	28.796848424817757	27.19212168916807	22.28385986607948
18-19	21.83437332794239	27.930285448405183	29.02346653236929	21.21187469128313
20-21	22.70473658868339	27.0465297046744	28.815468707973135	21.433264998669078
22-23	22.629424889524618	26.901389293153123	28.60898690610704	21.86019891121521
24-25	22.356128503681376	27.136534834756425	28.59566898123282	21.911667680329376
26-27	22.18676685511572	27.075299519441188	28.683039406659443	22.05489421878365
28-29	22.135435629540318	27.182997107153973	28.711486983065477	21.970080280240232
30-31	22.278472035751882	27.083228438081353	28.657537137067706	21.980762389099063
32-33	22.134473296423778	27.258937883881206	28.594634825494055	22.01195399420096
34-35	22.32583855187539	27.138672003350617	28.54558422407063	21.989905220703363
36-37	22.596630073407063	27.070852290489576	28.31660262988421	22.015915006219156
38-39	22.239317227538837	27.342272739859542	28.51090775626502	21.907502276336597
40-41	22.425218840423312	27.19295321252055	28.30985139586304	22.0719765511931
42-43	22.27164100166697	27.44411891646553	28.10947318266519	22.174766899202314
44-45	22.44282701236763	27.39491546174951	28.040609366203554	22.1216481596793
46-47	22.453488498082617	27.402384203668063	27.75505553988722	22.389071758362107
48-49	22.343996463684192	27.702773442407207	27.551388745637475	22.401841348271127
50-51	22.411394212762872	27.57771222721343	27.69646445758585	22.314429102437845
52-53	22.608662826042902	27.5876073299945	27.403512144283937	22.400217699678663
54-55	22.58878271918252	27.468108450429625	27.48130209215726	22.461806738230596
56-57	22.609417087603344	27.566673008025983	27.40816563004535	22.415744274325323
58-59	22.43401696425231	27.71494414827317	27.495511870469453	22.355527017005066
60-61	22.560587881766143	27.635972719837465	27.464497358021596	22.3389420403748
62-63	22.595565130640985	27.753921648235398	27.30613571397446	22.34437750714916
64-65	22.5542928508602	27.63646480176756	27.31756130114794	22.491681046224304
66-67	22.72315817436353	27.645568487873767	27.08273848978961	22.548534847973094
68-69	22.36796442369291	27.669470875140995	27.347787681919833	22.61477701924626
70-71	22.469094422374685	27.483717500081617	27.33845520436864	22.708732873175062
72-73	22.560174971639235	27.612851672495992	27.15769085226948	22.669282503595294
74-75	22.42797787121009	27.722328676595854	27.23981179943288	22.60988165276118
76-77	22.592871245395067	27.65782152510217	27.163395160189978	22.585912069312787
78-79	22.45900061733261	27.605732286495567	27.16294202499429	22.77232507117753
80-81	22.682874073657835	27.616209045925416	26.992405192474234	22.70851168794251
82-83	22.473565350472814	27.704559193023925	27.24076881676804	22.58110663973522
84-85	22.649030400378045	27.61179847552327	27.145191593701647	22.59397953039704
86-87	22.520267199427586	27.656884158741672	27.175308733469848	22.64753990836089
88-89	22.61350358305686	27.6160330235517	27.139473247032335	22.630990146359103
90-91	22.645288311423126	27.564695160747622	27.12343642286163	22.666580104967622
92-93	22.726939518963537	27.525422784646615	27.232533926973467	22.51510376941638
94-95	22.36473834869055	27.710164225748347	27.289805501046814	22.63529192451429
96-97	22.486218119266653	27.73563102467376	27.378557982262258	22.39959287379733
98-99	23.709249398573668	29.698219227986282	27.365521219578287	19.227010153861755
100	22.619959875885552	30.72632465709262	21.230352821386735	25.423362645635088
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3253.0
1	2770.0
2	3139.0
3	4210.5
4	5465.5
5	8663.0
6	11441.5
7	13375.0
8	14983.0
9	15364.0
10	14454.5
11	13405.0
12	14519.5
13	18689.0
14	28494.0
15	41846.5
16	53689.5
17	61896.0
18	64111.0
19	60547.0
20	54663.0
21	49033.0
22	47345.5
23	51166.0
24	59935.5
25	74936.5
26	95631.0
27	115717.5
28	133312.0
29	152995.5
30	173745.5
31	199817.0
32	230097.0
33	260687.0
34	286410.5
35	306312.5
36	332808.0
37	359724.0
38	375988.0
39	382752.0
40	392662.5
41	409929.5
42	423566.5
43	437431.0
44	453769.5
45	459063.5
46	457736.5
47	461363.0
48	465952.5
49	467741.5
50	459564.0
51	443915.5
52	427139.0
53	407692.5
54	386396.0
55	361101.5
56	334522.0
57	307651.0
58	278589.0
59	245095.0
60	205632.5
61	167034.5
62	133040.0
63	102696.0
64	77110.5
65	57874.5
66	43480.5
67	32660.0
68	24491.5
69	17647.5
70	12540.0
71	9009.5
72	6183.5
73	4014.0
74	2575.0
75	1654.0
76	1096.5
77	704.5
78	476.0
79	338.0
80	233.0
81	158.0
82	117.5
83	83.5
84	59.5
85	48.5
86	39.0
87	34.0
88	25.0
89	16.5
90	10.5
91	6.0
92	3.5
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.001933631327405679
4	0.012742804648623913
5	0.009337174698103098
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0015725246403938892
26-27	0.03627426007263218
28-29	0.08532727832529763
30-31	0.08302333750762528
32-33	0.03708614041761223
34-35	0.0013405785323609086
36-37	7.228422945379774
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.331031563985581E-5
56-57	5.1644804873015996E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.010722795849903433
74-75	0.03222387746529527
76-77	0.004552951665367696
78-79	0.0
80-81	0.0
82-83	0.0032206627717492843
84-85	0.03864585553169678
86-87	0.04232491343849433
88-89	0.01193682647649217
90-91	3.793395222034467E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	122.0
20-21	591.0
22-23	1160.0
24-25	2986.0
26-27	4537.0
28-29	7910.0
30-31	18517.0
32-33	24464.0
34-35	38273.0
36-37	47051.0
38-39	56791.0
40-41	62360.0
42-43	73968.0
44-45	90083.0
46-47	90264.0
48-49	79609.0
50-51	70641.0
52-53	68864.0
54-55	73602.0
56-57	77659.0
58-59	77984.0
60-61	76378.0
62-63	74597.0
64-65	79407.0
66-67	84569.0
68-69	84629.0
70-71	82987.0
72-73	79096.0
74-75	81259.0
76-77	81334.0
78-79	86396.0
80-81	87474.0
82-83	84867.0
84-85	85632.0
86-87	92255.0
88-89	100023.0
90-91	105843.0
92-93	114022.0
94-95	138127.0
96-97	401449.0
98-99	716838.0
100-101	7776371.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.91658633996013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.02617096955619	79.11684048271404
2	6.355594310336003	11.048131232321571
3	1.451278508630304	3.7842052139608318
4	0.5355221283971675	1.861830212391665
5	0.25366871600628577	1.102400942825358
6	0.13543391739239374	0.7062872264637013
7	0.07104315274595499	0.4322379823654741
8	0.04382278032585808	0.3047141175879641
9	0.029680615064930166	0.23217639677227125
>10	0.09588247533071294	1.2437973535089828
>50	0.001656347148279599	0.0987491524419831
>100	2.1493200741423034E-4	0.0357507951214705
>500	1.5573539144248145E-5	0.013414164699129445
>1k	1.5573519321093468E-5	0.019464726825585775
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.710051024349906E-6
2	0.0	4.3550255121749526E-5	0.0	0.0	8.710051024349906E-6
3	0.0	4.3550255121749526E-5	0.0	0.0	8.710051024349906E-6
4	0.0	7.839045921914916E-5	0.0	0.0	8.710051024349906E-6
5	0.0	7.839045921914916E-5	0.0	0.0	8.710051024349906E-6
6	4.3550255121749526E-5	7.839045921914916E-5	0.0	0.0	8.710051024349906E-6
7	5.2260306146099436E-5	7.839045921914916E-5	0.0	0.0	8.710051024349906E-6
8	6.097035717044934E-5	7.839045921914916E-5	0.0	0.0	8.710051024349906E-6
9	9.581056126784896E-5	1.1323066331654878E-4	0.0	0.0	2.6130153073049718E-5
10-11	9.581056126784896E-5	1.1323066331654878E-4	0.0	2.1775127560874767E-5	2.6130153073049718E-5
12-13	9.581056126784896E-5	1.2194071434089868E-4	0.0	3.919522960957458E-5	2.6130153073049718E-5
14-15	1.1323066331654878E-4	1.4371584190177344E-4	0.0	5.2260306146099436E-5	4.355025512174953E-5
16-17	1.3065076536524859E-4	1.480708674139484E-4	0.0	5.2260306146099436E-5	5.2260306146099436E-5
18-19	1.5242589292612337E-4	1.6984599497482316E-4	0.0	5.2260306146099436E-5	6.968040819479925E-5
20-21	1.6113594395047326E-4	1.8291107151134802E-4	0.0	5.2260306146099436E-5	6.968040819479925E-5
22-23	1.654909694626482E-4	2.0468619907222278E-4	0.0	6.532538268262429E-5	9.145553575567401E-5
24-25	1.6984599497482314E-4	2.3081635214527253E-4	0.0	1.0887563780437383E-4	1.2194071434089868E-4
26-27	1.742010204869981E-4	2.525914797061473E-4	0.0	1.3500579087742355E-4	1.3065076536524859E-4
28-29	1.742010204869981E-4	2.6130153073049717E-4	0.0	2.395264031696224E-4	1.3065076536524859E-4
30-31	1.742010204869981E-4	3.048517858522467E-4	0.0	6.009935206801435E-4	1.3500579087742355E-4
32-33	1.7855604599917307E-4	3.048517858522467E-4	0.0	0.00138054308735946	1.393608163895985E-4
34-35	2.0468619907222278E-4	3.3969198994964633E-4	0.0	0.002591240179744097	1.393608163895985E-4
36-37	2.2646132663309756E-4	3.7453219404704597E-4	0.0	0.00463374714495415	1.4371584190177344E-4
38-39	2.395264031696224E-4	3.7453219404704597E-4	0.0	0.007782430590256641	1.480708674139484E-4
40-41	2.4388142868179735E-4	4.3985757672967025E-4	0.0	0.011353551510240102	1.480708674139484E-4
42-43	2.4388142868179735E-4	4.485676277540202E-4	0.0	0.01480708674139484	1.5678091843829831E-4
44-45	2.4388142868179735E-4	4.877628573635947E-4	0.0	0.01922743763625242	1.6984599497482314E-4
46-47	2.4388142868179735E-4	5.400231635096941E-4	0.0	0.023930865189401365	1.742010204869981E-4
48-49	2.4823645419397235E-4	5.487332145340441E-4	0.0	0.028255405522991096	1.9162112253569792E-4
50-51	2.525914797061473E-4	6.097035717044934E-4	0.0	0.032784632055653046	2.0033117356004783E-4
52-53	2.525914797061473E-4	6.227686482410183E-4	0.0	0.037270308333193246	2.1775127560874765E-4
54-55	2.5694650521832223E-4	6.314786992653683E-4	0.0	0.041725499432148226	2.3517137765744744E-4
56-57	2.6130153073049717E-4	6.40188750289718E-4	0.0	0.04669893856705202	2.395264031696224E-4
58-59	2.6130153073049717E-4	6.532538268262429E-4	0.0	0.05163753749785842	2.4388142868179735E-4
60-61	2.74366607267022E-4	6.750289543871178E-4	0.0	0.05635403012754389	2.525914797061473E-4
62-63	2.961417348278968E-4	6.880940309236425E-4	0.0	0.06099213229801022	2.6130153073049717E-4
64-65	2.961417348278968E-4	7.839045921914915E-4	0.0	0.06586976087164616	2.6130153073049717E-4
66-67	3.222718879009465E-4	7.839045921914915E-4	0.0	0.0707299693432334	2.700115817548471E-4
68-69	3.222718879009465E-4	7.926146432158415E-4	0.0	0.0759124497027216	2.8307665829137193E-4
70-71	3.2662691341312145E-4	7.926146432158415E-4	0.0	0.08045038628640791	2.874316838035469E-4
72-73	3.3969198994964633E-4	7.926146432158415E-4	0.0	0.08381246598180697	3.222718879009465E-4
74-75	3.3969198994964633E-4	7.926146432158415E-4	0.0	0.08649516169730673	3.222718879009465E-4
76-77	3.4404701546182127E-4	8.013246942401913E-4	0.0	0.08914301720870911	3.222718879009465E-4
78-79	3.484020409739962E-4	8.013246942401913E-4	0.0	0.09073260152065296	3.222718879009465E-4
80-81	3.484020409739962E-4	8.013246942401913E-4	0.0	0.09126826965865048	3.3969198994964633E-4
82-83	3.5711209199834615E-4	8.056797197523663E-4	0.0	0.09169506215884363	3.5711209199834615E-4
84-85	3.875972705835708E-4	8.100347452645412E-4	0.0	0.09195636368957413	3.5711209199834615E-4
86-87	3.9195229609574573E-4	8.36164898337591E-4	0.0	0.0920652393273785	3.6582214302269603E-4
88	4.0937239814444555E-4	8.710051024349906E-4	0.0	0.0920826594294272	3.7453219404704597E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16070	0.0	47.142334	1
GTATCAA	29845	0.0	35.76018	1
TCAACGC	32205	0.0	32.132317	4
ATCAACG	32640	0.0	31.757048	3
CAACGCA	32990	0.0	31.428541	5
AACGCAG	34015	0.0	30.528845	6
TATCAAC	34695	0.0	30.011307	2
ACGCAGA	39000	0.0	26.593826	7
CGCAGAG	40170	0.0	25.851099	8
GCAGAGT	46735	0.0	22.164968	9
GTACATG	34875	0.0	20.744148	1
GAGTACT	26545	0.0	20.652433	12-13
TACATGG	34615	0.0	20.37007	2
ACATGGG	34660	0.0	19.949797	3
CAGAGTA	46225	0.0	19.268124	10-11
AGAGTAC	42975	0.0	18.884468	10-11
GTACTTT	29615	0.0	18.541054	14-15
GTGGTAT	7365	0.0	18.244074	1
CATGGGG	28365	0.0	17.534376	4
AGTACTT	30770	0.0	16.382187	12-13
>>END_MODULE