FastQCFastQC Report
Fri 27 May 2016
SRR2138927_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138927_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8213920
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106000.049.310681
CTTATAC70150.037.0142631
GTATCAA193050.036.385491
TCAACGC227000.030.1607044
ATCAACG229050.029.8728473
CAACGCA233150.029.3053635
TATCAAC240100.028.8150622
AACGCAG239650.028.5961196
TATACAC107850.027.8006213
TTATACA108650.025.4551942
ACGCAGA274100.025.0020437
CGCAGAG279100.024.5692848
TACACAT135650.021.101345
GCAGAGT327500.020.8995579
ACACATC128100.020.7588376
ACATCTC129750.020.6577688
GTACATG234500.020.071541
GAGTACT197050.019.85644512-13
TACATGG236600.019.4108052
GTGGTAT53300.019.2800221
Produced by FastQC (version 0.11.5)