##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138927_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8213920
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71843638603736	33.0	33.0	33.0	33.0	33.0
2	32.717192034984514	33.0	33.0	33.0	33.0	33.0
3	32.717166833862514	33.0	33.0	33.0	33.0	33.0
4	36.64661903208212	37.0	37.0	37.0	37.0	37.0
5	36.6629774821279	37.0	37.0	37.0	37.0	37.0
6	36.58820258780217	37.0	37.0	37.0	37.0	37.0
7	36.63400787930732	37.0	37.0	37.0	37.0	37.0
8	36.64040993825116	37.0	37.0	37.0	37.0	37.0
9	36.64864291349319	37.0	37.0	37.0	37.0	37.0
10-11	36.60946113426963	37.0	37.0	37.0	37.0	37.0
12-13	36.582628878781385	37.0	37.0	37.0	37.0	37.0
14-15	38.699953980559826	40.0	37.0	40.0	37.0	40.0
16-17	38.64824505230146	40.0	37.0	40.0	37.0	40.0
18-19	38.67773407824766	40.0	37.0	40.0	37.0	40.0
20-21	38.69882881718006	40.0	37.0	40.0	37.0	40.0
22-23	38.554114627742024	40.0	37.0	40.0	37.0	40.0
24-25	38.70261616186785	40.0	37.0	40.0	37.0	40.0
26-27	38.49147931682358	40.0	37.0	40.0	37.0	40.0
28-29	38.47851590517445	40.0	37.0	40.0	37.0	40.0
30-31	38.45946146122509	40.0	37.0	40.0	37.0	40.0
32-33	38.46023322525058	40.0	37.0	40.0	37.0	40.0
34-35	38.37643768513048	40.0	37.0	40.0	37.0	40.0
36-37	33.443814070173794	40.0	37.0	40.0	8.5	40.0
38-39	37.31635787523828	40.0	37.0	40.0	33.0	40.0
40-41	37.882433907580136	40.0	37.0	40.0	35.0	40.0
42-43	38.09788093195614	40.0	37.0	40.0	35.0	40.0
44-45	38.10894444316834	40.0	37.0	40.0	37.0	40.0
46-47	38.009270190802226	40.0	37.0	40.0	33.0	40.0
48-49	37.9918498868087	40.0	37.0	40.0	33.0	40.0
50-51	37.9355034020089	40.0	37.0	40.0	33.0	40.0
52-53	37.87571127831198	40.0	37.0	40.0	33.0	40.0
54-55	37.796386174324674	40.0	37.0	40.0	33.0	40.0
56-57	37.71922833873437	40.0	37.0	40.0	33.0	40.0
58-59	37.58809384007293	40.0	37.0	40.0	33.0	40.0
60-61	37.39688618250638	38.5	37.0	40.0	33.0	40.0
62-63	37.18464604163579	37.0	37.0	40.0	33.0	40.0
64-65	36.98875731551982	37.0	37.0	40.0	33.0	40.0
66-67	36.72900293453094	37.0	37.0	40.0	33.0	40.0
68-69	36.460862139580286	37.0	37.0	40.0	33.0	40.0
70-71	36.19707833016629	37.0	37.0	38.5	33.0	40.0
72-73	35.944374262359645	37.0	37.0	37.0	33.0	40.0
74-75	35.66353122669989	37.0	35.0	37.0	33.0	40.0
76-77	33.82613858429083	35.0	33.0	37.0	27.0	37.0
78-79	35.2756788098701	37.0	33.0	37.0	33.0	37.0
80-81	35.29588136927222	37.0	33.0	37.0	33.0	37.0
82-83	35.363067027644746	37.0	33.0	37.0	33.0	37.0
84-85	35.20610605575018	37.0	33.0	37.0	33.0	37.0
86-87	35.13560108010164	37.0	33.0	37.0	33.0	37.0
88-89	35.048425423352484	37.0	33.0	37.0	33.0	37.0
90-91	34.91353566097159	37.0	33.0	37.0	33.0	37.0
92-93	34.89571926829393	37.0	33.0	37.0	33.0	37.0
94-95	34.856605477675686	37.0	33.0	37.0	33.0	37.0
96-97	34.83253656561082	37.0	33.0	37.0	33.0	37.0
98-99	34.7896640896695	37.0	33.0	37.0	33.0	37.0
100	34.73640212244679	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	9.0
20	47.0
21	264.0
22	927.0
23	2669.0
24	6454.0
25	12677.0
26	22767.0
27	35972.0
28	54130.0
29	76450.0
30	104787.0
31	140749.0
32	189016.0
33	257043.0
34	361387.0
35	561811.0
36	1083338.0
37	2531809.0
38	2676271.0
39	95340.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.0271831232834	20.464613728889493	14.581673549291935	23.926529598535172
2	14.152852718312328	21.04337514852835	42.25836141574303	22.54541071741629
3	17.592279093924322	26.672807588153084	30.983201219515905	24.751712098406685
4	12.163030122897348	18.639526718633583	39.17424397485434	30.023199183614725
5	13.055898434047075	37.91549547518089	35.81314638751095	13.21545970326109
6	29.129027309737616	38.51852708572764	19.01999289011824	13.332452714416505
7	25.748570718974616	33.02358678925531	23.436824317743536	17.79101817402653
8	24.049832479498217	36.954365272610396	21.419212751037264	17.57658949685412
9	25.22414389231938	17.040730856886846	21.31678906052165	36.41833619027212
10-11	23.791301838829693	26.875663507801388	29.235121598457255	20.09791305491166
12-13	24.83720318678536	24.45211299842219	29.12659241872334	21.584091396069113
14-15	22.018640308159807	25.76290613982118	26.70978655745369	25.50866699456532
16-17	21.572641321074467	29.096235415002823	27.270347166760818	22.06077609716189
18-19	21.692297952743637	28.044776428307067	29.0178380115706	21.245087607378693
20-21	22.3750764572161	27.23791975985616	28.933346352162907	21.453657430764824
22-23	22.106277696966316	27.359400741658053	28.794428834784508	21.73989272659112
24-25	22.04329713182806	27.452631584074943	28.80426039607445	21.699810888022547
26-27	21.97493583152443	27.506534140675576	28.818584104497507	21.699945923302487
28-29	21.746500932688182	27.466524926118346	28.910314643890594	21.876659497302875
30-31	21.990438273668488	27.392366784276444	28.913312989096205	21.703881952958856
32-33	21.635042548927814	27.391548559427708	29.079866980268953	21.893541911375525
34-35	21.81853646272465	27.462233767303047	28.852885351633823	21.86634441833848
36-37	22.05400553648277	27.521509347596584	28.692255401677443	21.732229714243207
38-39	21.838532783933793	27.488886917293755	28.849709751265152	21.8228705475073
40-41	22.007554747754277	27.391073563924483	28.768707325776845	21.832664362544396
42-43	22.025295631054096	27.4655933376058	28.644854762984174	21.864256268355934
44-45	21.938882303542435	27.699318958190915	28.436599989823918	21.92519874844273
46-47	22.218786484422935	27.516143662397685	27.979085334325944	22.285984518853436
48-49	22.065969501148984	27.668783971670823	28.000864950340613	22.264381576839572
50-51	21.9916145087701	27.829293356472697	28.033112667832928	22.145979466924278
52-53	22.12480830387424	27.82884150782995	28.02525063380999	22.021099554485822
54-55	22.093410700530843	27.759704676988328	27.875471423226088	22.27141319925474
56-57	22.257096195040084	27.94435978360994	27.632348332587764	22.166195688762212
58-59	21.995928849245615	28.146079523890222	27.93962657289166	21.9183650539725
60-61	22.248237646400558	28.244255688238244	27.60859693592832	21.898909729432873
62-63	22.122824616849762	28.42018017861757	27.507301471324556	21.949693733208107
64-65	22.116702066342235	28.24489295202386	27.550361369361248	22.088043612272656
66-67	22.235314529403542	28.108922593061443	27.489233611025316	22.16652926650971
68-69	22.167353471581567	27.985048443706432	27.625712223834743	22.221885860877258
70-71	22.178237402603617	27.82776596575757	27.647601573403318	22.346395058235487
72-73	22.233667423881776	27.89360364457253	27.533257292322304	22.339471639223387
74-75	22.122070335299465	28.01498740498235	27.55222237929888	22.310719880419306
76-77	22.249930060415956	27.879102514459834	27.56099114464488	22.309976280479322
78-79	22.188948813082053	27.896121684361606	27.460995543024296	22.453933959532044
80-81	22.3126441803276	27.930558819321938	27.43117865912341	22.325618341227052
82-83	22.219675859588612	27.94371918079174	27.603836703431078	22.23276825618857
84-85	22.34077902732159	27.879428666603022	27.508687534547477	22.27110477152791
86-87	22.205120508522143	27.923200398850394	27.571298521863696	22.300380570763764
88-89	22.24674905755733	27.877983133291913	27.51621273705637	22.35905507209439
90-91	22.26925368779233	27.93633470546977	27.5013783121167	22.293033294621207
92-93	22.2753519369461	27.901535561852995	27.563146171015756	22.259966330185144
94-95	22.12649061859645	27.977759542430867	27.58382685022313	22.31192298874955
96-97	22.205759189780526	28.06672452762629	27.664716736693627	22.062799545899555
98-99	23.350167365524673	29.955609750579637	27.829502438644553	18.864720445251134
100	22.237642555172027	31.016640551487924	21.66959838706093	25.076118506279123
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2077.0
1	1761.0
2	2149.5
3	3058.5
4	4135.5
5	6554.5
6	8602.5
7	10012.0
8	11084.5
9	11190.5
10	10442.0
11	9630.5
12	10334.5
13	13401.0
14	20560.5
15	30272.0
16	38819.0
17	44762.5
18	46770.0
19	45080.5
20	41753.0
21	38559.5
22	37479.0
23	40098.5
24	46581.5
25	57115.0
26	71510.5
27	87619.0
28	104693.5
29	123726.5
30	142747.0
31	161819.5
32	182540.5
33	202050.0
34	219305.0
35	234083.0
36	250075.0
37	266849.5
38	279104.0
39	287743.5
40	296010.5
41	304439.0
42	309959.5
43	315708.0
44	322601.0
45	327290.5
46	329523.5
47	331953.5
48	342908.5
49	345124.5
50	333146.5
51	322479.5
52	309080.5
53	289635.5
54	267612.5
55	245116.5
56	224380.5
57	203347.5
58	179890.5
59	155282.5
60	129326.0
61	104999.5
62	83270.5
63	64152.5
64	47977.5
65	36144.0
66	27786.0
67	21354.5
68	16006.5
69	11526.0
70	8258.0
71	5977.0
72	4110.5
73	2821.5
74	1923.5
75	1280.0
76	847.5
77	522.0
78	334.0
79	234.5
80	161.0
81	109.5
82	77.0
83	56.5
84	43.0
85	34.0
86	27.5
87	19.5
88	11.0
89	6.0
90	5.0
91	5.0
92	4.5
93	3.5
94	2.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.00205748290706508
4	0.013769308685743225
5	0.009423028225256636
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0016805341175825882
26-27	0.0367769033737723
28-29	0.08536173589500604
30-31	0.08305305367584179
32-33	0.037393073927616566
34-35	0.0013477202056890578
36-37	7.248204812216316
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.617971866358034E-5
56-57	4.619261343157139E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.011110267093660618
74-75	0.03320423914612271
76-77	0.0044482051997496875
78-79	0.0
80-81	0.0
82-83	0.0033119770223054225
84-85	0.03969953389778734
86-87	0.043514450786548864
88-89	0.01229080393942064
90-91	1.5471439915309338E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	111.0
20-21	410.0
22-23	950.0
24-25	2672.0
26-27	3497.0
28-29	5929.0
30-31	14186.0
32-33	18456.0
34-35	28844.0
36-37	35542.0
38-39	43599.0
40-41	47435.0
42-43	55710.0
44-45	67708.0
46-47	67242.0
48-49	60246.0
50-51	54305.0
52-53	53189.0
54-55	60215.0
56-57	65476.0
58-59	65836.0
60-61	66648.0
62-63	67102.0
64-65	68959.0
66-67	75138.0
68-69	64421.0
70-71	62108.0
72-73	59251.0
74-75	59928.0
76-77	60146.0
78-79	64251.0
80-81	65475.0
82-83	63072.0
84-85	63544.0
86-87	67263.0
88-89	72495.0
90-91	76769.0
92-93	82082.0
94-95	98717.0
96-97	278310.0
98-99	502212.0
100-101	5444471.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.45558360276877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.26232779745362	79.81400138473494
2	6.2516138660542415	10.9347707822987
3	1.401644663627537	3.6774495618375833
4	0.5181635860027685	1.8126519526230234
5	0.22107568998158672	0.9667151743862222
6	0.11924373006574787	0.6257118002322603
7	0.060921592362488654	0.3729553389849997
8	0.0418192564677588	0.29258619841773664
9	0.029218446430077538	0.22997846560577997
>10	0.09282632196551137	1.1902723382329263
>50	9.908767560135412E-4	0.05642377244085188
>100	1.5417283261439169E-4	0.026483230204920175
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2174455071391005E-5
2	0.0	2.434891014278201E-5	0.0	0.0	1.2174455071391005E-5
3	0.0	2.434891014278201E-5	0.0	0.0	1.2174455071391005E-5
4	0.0	8.522118549973703E-5	0.0	0.0	2.434891014278201E-5
5	0.0	8.522118549973703E-5	0.0	1.2174455071391005E-5	2.434891014278201E-5
6	1.9479128114225608E-4	8.522118549973703E-5	0.0	3.6523365214173013E-5	2.434891014278201E-5
7	1.9479128114225608E-4	8.522118549973703E-5	0.0	3.6523365214173013E-5	3.6523365214173013E-5
8	1.9479128114225608E-4	1.0957009564251905E-4	0.0	3.6523365214173013E-5	3.6523365214173013E-5
9	1.9479128114225608E-4	1.7044237099947407E-4	0.0	7.304673042834603E-5	3.6523365214173013E-5
10-11	2.0087850867795158E-4	1.7044237099947407E-4	0.0	9.739564057112804E-5	3.6523365214173013E-5
12-13	2.0696573621364707E-4	1.7044237099947407E-4	0.0	1.2174455071391005E-4	3.6523365214173013E-5
14-15	2.191401912850381E-4	2.191401912850381E-4	0.0	1.5218068839238755E-4	4.869782028556402E-5
16-17	2.313146463564291E-4	2.191401912850381E-4	0.0	1.5826791592808305E-4	4.869782028556402E-5
18-19	2.313146463564291E-4	2.4348910142782008E-4	0.0	1.5826791592808305E-4	6.087227535695502E-5
20-21	2.313146463564291E-4	2.4348910142782008E-4	0.0	1.6435514346377854E-4	7.304673042834603E-5
22-23	2.3740187389212458E-4	2.556635564992111E-4	0.0	2.2522741882073356E-4	9.130841303543253E-5
24-25	2.4348910142782008E-4	2.9827414924907963E-4	0.0	2.4348910142782008E-4	1.0957009564251905E-4
26-27	2.4348910142782008E-4	3.2871028692755714E-4	0.0	2.9827414924907963E-4	1.0957009564251905E-4
28-29	2.4348910142782008E-4	3.2871028692755714E-4	6.0872275356955025E-6	3.956697898202077E-4	1.0957009564251905E-4
30-31	2.4957632896351555E-4	3.8958256228451216E-4	1.2174455071391005E-5	7.852523521047198E-4	1.2783177824960556E-4
32-33	2.617507840349066E-4	3.8958256228451216E-4	1.2174455071391005E-5	0.001600940841887917	1.4609346085669205E-4
34-35	3.043613767847751E-4	3.8958256228451216E-4	1.2174455071391005E-5	0.0028001246664199307	1.4609346085669205E-4
36-37	3.043613767847751E-4	3.8958256228451216E-4	1.2174455071391005E-5	0.004693252430021232	1.4609346085669205E-4
38-39	3.043613767847751E-4	4.626292927128582E-4	1.2174455071391005E-5	0.008625601418080527	1.5826791592808305E-4
40-41	3.043613767847751E-4	5.113271129984223E-4	1.2174455071391005E-5	0.012539688723532734	1.5826791592808305E-4
42-43	3.043613767847751E-4	5.356760231412042E-4	1.2174455071391005E-5	0.01617376356234295	1.5826791592808305E-4
44-45	3.1653583185616615E-4	5.539377057482908E-4	1.2174455071391005E-5	0.02053221847790093	1.5826791592808305E-4
46-47	3.2871028692755714E-4	5.843738434267682E-4	1.2174455071391005E-5	0.02506720299199408	1.6435514346377854E-4
48-49	3.2871028692755714E-4	6.026355260338547E-4	1.2174455071391005E-5	0.02932826226698093	1.8261682607086506E-4
50-51	3.2871028692755714E-4	7.000311666049827E-4	1.2174455071391005E-5	0.03392411905643103	1.8261682607086506E-4
52-53	3.2871028692755714E-4	7.061183941406783E-4	1.2174455071391005E-5	0.03786255527202602	1.9479128114225605E-4
54-55	3.2871028692755714E-4	7.061183941406783E-4	1.2174455071391005E-5	0.041983608313691884	2.1305296374934257E-4
56-57	3.4088474199894813E-4	7.426417593548513E-4	1.2174455071391005E-5	0.046634250150963244	2.252274188207336E-4
58-59	3.4088474199894813E-4	7.730778970333287E-4	1.2174455071391005E-5	0.05112053684477083	2.313146463564291E-4
60-61	3.956697898202077E-4	7.791651245690243E-4	1.2174455071391005E-5	0.0556250852211855	2.313146463564291E-4
62-63	4.626292927128582E-4	7.913395796404153E-4	1.2174455071391005E-5	0.059971365681672086	2.313146463564291E-4
64-65	4.626292927128582E-4	8.643863100687613E-4	1.2174455071391005E-5	0.06458548415372928	2.313146463564291E-4
66-67	4.7480374778424917E-4	8.887352202115433E-4	1.2174455071391005E-5	0.06977180201414185	2.3740187389212458E-4
68-69	4.7480374778424917E-4	9.069969028186298E-4	1.2174455071391005E-5	0.0745807117673413	2.5566355649921113E-4
70-71	4.991526579270312E-4	9.130841303543253E-4	1.2174455071391005E-5	0.07906091123361318	3.043613767847751E-4
72-73	5.478504782125952E-4	9.130841303543253E-4	1.2174455071391005E-5	0.08299934744920817	3.2871028692755714E-4
74-75	5.478504782125952E-4	9.130841303543253E-4	1.2174455071391005E-5	0.08609165903734148	3.2871028692755714E-4
76-77	5.600249332839862E-4	9.374330404971074E-4	1.2174455071391005E-5	0.08892221984143989	3.2871028692755714E-4
78-79	5.721993883553772E-4	9.496074955684983E-4	1.2174455071391005E-5	0.09091883047314803	3.2871028692755714E-4
80-81	5.721993883553772E-4	9.556947231041939E-4	1.2174455071391005E-5	0.09155190213686035	3.5305919707033913E-4
82-83	5.721993883553772E-4	9.678691781755849E-4	1.2174455071391005E-5	0.0920267058846446	3.5305919707033913E-4
84-85	5.721993883553772E-4	9.739564057112803E-4	1.2174455071391005E-5	0.0921849738005727	3.5305919707033913E-4
86-87	5.843738434267682E-4	9.861308607826713E-4	1.2174455071391005E-5	0.09225802053100103	3.5305919707033913E-4
88	6.087227535695502E-4	0.0010104797709254534	1.2174455071391005E-5	0.09227019498607242	3.5305919707033913E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10600	0.0	49.31068	1
CTTATAC	7015	0.0	37.014263	1
GTATCAA	19305	0.0	36.38549	1
TCAACGC	22700	0.0	30.160704	4
ATCAACG	22905	0.0	29.872847	3
CAACGCA	23315	0.0	29.305363	5
TATCAAC	24010	0.0	28.815062	2
AACGCAG	23965	0.0	28.596119	6
TATACAC	10785	0.0	27.800621	3
TTATACA	10865	0.0	25.455194	2
ACGCAGA	27410	0.0	25.002043	7
CGCAGAG	27910	0.0	24.569284	8
TACACAT	13565	0.0	21.10134	5
GCAGAGT	32750	0.0	20.899557	9
ACACATC	12810	0.0	20.758837	6
ACATCTC	12975	0.0	20.657768	8
GTACATG	23450	0.0	20.07154	1
GAGTACT	19705	0.0	19.856445	12-13
TACATGG	23660	0.0	19.410805	2
GTGGTAT	5330	0.0	19.280022	1
>>END_MODULE