FastQCFastQC Report
Fri 27 May 2016
SRR2138926_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138926_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9297076
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121350.052.067521
GTATCAA225300.038.06961
TCAACGC275200.030.627444
ATCAACG275450.030.6153563
CAACGCA281050.030.0009785
TATCAAC286600.029.6165052
AACGCAG290050.029.0680436
ACGCAGA329500.025.6135337
CGCAGAG340750.024.7803278
GTGGTAT58350.022.2959791
GCAGAGT393150.021.3805569
GTACATG287100.020.3692421
TGGTATC57350.020.3202152
TACATGG282500.020.2058982
GAGTACT229150.019.80182312-13
ACATGGG287150.019.2736243
CAGAGTA380150.019.1415610-11
AGAGTAC352850.018.92951210-11
CATGGGG215000.017.79814
GTACTTT258250.017.55463614-15
Produced by FastQC (version 0.11.5)