##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138926_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9297076
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71774458980436	33.0	33.0	33.0	33.0	33.0
2	32.712686763020976	33.0	33.0	33.0	33.0	33.0
3	32.710376144069386	33.0	33.0	33.0	33.0	33.0
4	36.63922054633091	37.0	37.0	37.0	37.0	37.0
5	36.657535229355986	37.0	37.0	37.0	37.0	37.0
6	36.58457013796596	37.0	37.0	37.0	37.0	37.0
7	36.631180706708214	37.0	37.0	37.0	37.0	37.0
8	36.63558413419445	37.0	37.0	37.0	37.0	37.0
9	36.643218792661266	37.0	37.0	37.0	37.0	37.0
10-11	36.60352023582468	37.0	37.0	37.0	37.0	37.0
12-13	36.57707659913719	37.0	37.0	37.0	37.0	37.0
14-15	38.68701686422699	40.0	37.0	40.0	37.0	40.0
16-17	38.63719404896766	40.0	37.0	40.0	37.0	40.0
18-19	38.67087426197226	40.0	37.0	40.0	37.0	40.0
20-21	38.69418289492532	40.0	37.0	40.0	37.0	40.0
22-23	38.552654921851584	40.0	37.0	40.0	37.0	40.0
24-25	38.70179597199275	40.0	37.0	40.0	37.0	40.0
26-27	38.49678750399832	40.0	37.0	40.0	37.0	40.0
28-29	38.48478575763811	40.0	37.0	40.0	37.0	40.0
30-31	38.46511781034276	40.0	37.0	40.0	37.0	40.0
32-33	38.46023065641082	40.0	37.0	40.0	37.0	40.0
34-35	38.37404339217781	40.0	37.0	40.0	37.0	40.0
36-37	33.46856584258849	40.0	37.0	40.0	8.5	40.0
38-39	37.33861836990627	40.0	37.0	40.0	33.0	40.0
40-41	37.893712422649784	40.0	37.0	40.0	35.0	40.0
42-43	38.10373001425192	40.0	37.0	40.0	35.0	40.0
44-45	38.11128451271328	40.0	37.0	40.0	37.0	40.0
46-47	38.01137905398984	40.0	37.0	40.0	33.0	40.0
48-49	37.993105165967584	40.0	37.0	40.0	33.0	40.0
50-51	37.95334834526875	40.0	37.0	40.0	33.0	40.0
52-53	37.88755697915457	40.0	37.0	40.0	33.0	40.0
54-55	37.805812418426044	40.0	37.0	40.0	33.0	40.0
56-57	37.732956020359566	40.0	37.0	40.0	33.0	40.0
58-59	37.59400232103585	40.0	37.0	40.0	33.0	40.0
60-61	37.40819362526891	38.5	37.0	40.0	33.0	40.0
62-63	37.18573316177563	37.0	37.0	40.0	33.0	40.0
64-65	36.9791157515253	37.0	37.0	40.0	33.0	40.0
66-67	36.7135445439383	37.0	37.0	40.0	33.0	40.0
68-69	36.43969410358647	37.0	37.0	40.0	33.0	40.0
70-71	36.17211255192018	37.0	37.0	38.5	33.0	40.0
72-73	35.91107425042422	37.0	37.0	37.0	33.0	40.0
74-75	35.62641298074756	37.0	35.0	37.0	33.0	40.0
76-77	33.7836587343167	35.0	33.0	37.0	27.0	37.0
78-79	35.243069691809445	37.0	33.0	37.0	33.0	37.0
80-81	35.26845410938715	37.0	33.0	37.0	33.0	37.0
82-83	35.33576328549243	37.0	33.0	37.0	33.0	37.0
84-85	35.1823315208552	37.0	33.0	37.0	33.0	37.0
86-87	35.113077829460394	37.0	33.0	37.0	33.0	37.0
88-89	35.02574378447861	37.0	33.0	37.0	33.0	37.0
90-91	34.88970462146664	37.0	33.0	37.0	33.0	37.0
92-93	34.8688002827736	37.0	33.0	37.0	33.0	37.0
94-95	34.83141209064429	37.0	33.0	37.0	33.0	37.0
96-97	34.804252154548706	37.0	33.0	37.0	33.0	37.0
98-99	34.764116680484854	37.0	33.0	37.0	33.0	37.0
100	34.709628007445374	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	12.0
20	48.0
21	226.0
22	938.0
23	2867.0
24	6946.0
25	13860.0
26	24980.0
27	40631.0
28	60463.0
29	86049.0
30	119264.0
31	159644.0
32	214969.0
33	291500.0
34	410562.0
35	638432.0
36	1236547.0
37	2883324.0
38	3003217.0
39	102595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.45590506090302	20.342137678556142	14.550112314882657	23.651844945658183
2	14.365914616595582	21.180465772249253	42.040734097473226	22.412885513681935
3	17.735655199890545	26.767967947984317	30.752839014517548	24.743537837607597
4	12.22021784342731	18.460020316791777	39.545248537532004	29.774513302248906
5	13.107830620067023	37.95175402692509	35.686665475503695	13.253749877504198
6	29.111518503236933	38.49191939487211	19.170661829590294	13.225900272300667
7	25.916718331656103	33.088564619671814	23.587986158228674	17.406730890443402
8	24.1765475510795	36.74768281984572	21.418217942931737	17.65755168614304
9	25.623927350921942	17.00251778085927	21.407924383967604	35.96563048425118
10-11	23.902493644238255	26.934371623938535	29.230453746963025	19.932680984860184
12-13	24.938469901719635	24.592404106409372	29.156021742750088	21.313104249120908
14-15	22.063168032615845	25.789619230820527	26.992852376381567	25.15436036018206
16-17	21.430135668461784	28.995971421552326	27.56975956741668	22.00413334256921
18-19	21.507052324838476	28.107122067196183	29.363264320954247	21.0225612870111
20-21	22.387597661339296	27.22559160972584	29.27505250563376	21.111758223301106
22-23	22.14116298249858	27.173558411220732	29.065838487669023	21.619440118611664
24-25	21.862485758433007	27.35062446100722	29.137604563455593	21.64928521710418
26-27	21.740834739911662	27.459881027050876	29.058002703989384	21.741281529048077
28-29	21.70183127067801	27.545112570483845	29.15407208743822	21.598984071399922
30-31	21.99321707302511	27.382188536202985	29.016776534306484	21.607817856465424
32-33	21.834810178780728	27.552933085109732	28.967665021498483	21.644591714611057
34-35	21.980699704452995	27.58821215386457	28.897113442549976	21.533974699132454
36-37	22.106369609887526	27.51351389335399	28.70767886620909	21.672437630549393
38-39	21.81432932292061	27.666677694783015	28.875119029953783	21.643873952342595
40-41	22.084554359373843	27.541944395933115	28.69046147825601	21.68303976643703
42-43	21.953894057314226	27.605892161421792	28.56720288408134	21.873010897182642
44-45	22.000089079667845	27.770335913859963	28.349437267035793	21.880137739436403
46-47	22.138821153841942	27.697271236156663	28.030546579089737	22.133361030911654
48-49	22.07281962863399	27.821159486503795	28.06543301030192	22.04058787456029
50-51	22.05365390998219	27.961291627861364	27.91451403538998	22.070540426766467
52-53	22.228572098650638	27.89422730702852	27.78417747218662	22.09302312213422
54-55	22.195027106424885	27.791671186430083	27.812717751782273	22.20058395536276
56-57	22.18454713588402	27.930789720642764	27.72298507532328	22.16167806814994
58-59	22.13193755369105	27.96481729508868	27.835591357981652	22.067653793238616
60-61	22.280332428487483	27.962551457910685	27.72556642143529	22.031549692166546
62-63	22.21321834274998	28.076059820586025	27.684514495263805	22.026207341400195
64-65	22.187330815188073	27.946379555644867	27.704177190126266	22.16211243904079
66-67	22.266575006135596	27.976165104563805	27.54123297015216	22.21602691914844
68-69	22.18975906751145	27.932652107355267	27.682047034853767	22.195541790279513
70-71	22.19378348024818	27.757669947456236	27.69789750175032	22.350649070545266
72-73	22.261695934049747	27.857401626449928	27.56449400821473	22.3164084312856
74-75	22.113098678337742	27.947766436442496	27.584135424188595	22.35499946103117
76-77	22.2284777630744	27.862314256259214	27.55028348128142	22.358924499384962
78-79	22.19887769231601	27.868392816775252	27.489365552111817	22.44336393879692
80-81	22.316148827595118	27.89015567469802	27.43105096569989	22.362644532006975
82-83	22.272917690785064	27.892442441583913	27.58644124528536	22.24819862234566
84-85	22.348179678921223	27.861893340471227	27.463449468135554	22.326477512471996
86-87	22.222316820545622	27.919205966505455	27.546465661784154	22.312011551164773
88-89	22.227633655801917	27.88756537229028	27.530303244105152	22.354497727802652
90-91	22.317210102535938	27.905105034650656	27.52431377477109	22.25337108804231
92-93	22.321289134656226	27.8348112533726	27.58211988041981	22.26177973155136
94-95	22.152191715919393	27.9504624405019	27.560554447492635	22.33679139608607
96-97	22.226577299996922	28.074284344441438	27.624347558612495	22.074790796949145
98-99	23.36095877904065	29.91385550438438	27.82690506855155	18.898280648023416
100	22.240623403902475	31.0098970762844	21.667299924961718	25.082179594851407
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2693.0
1	2301.5
2	2790.5
3	3858.5
4	5132.0
5	8113.5
6	10593.0
7	12146.5
8	13349.0
9	13589.5
10	12720.5
11	11621.5
12	12590.5
13	16671.0
14	25613.5
15	37704.5
16	48466.0
17	55731.5
18	57696.0
19	54779.0
20	49913.5
21	45006.5
22	42605.5
23	44848.0
24	51644.5
25	63761.5
26	80026.5
27	98227.5
28	118036.5
29	140154.5
30	160731.5
31	180937.5
32	203017.5
33	223899.0
34	245177.5
35	265958.5
36	288009.5
37	307302.5
38	319360.0
39	330149.0
40	341900.0
41	350813.5
42	356802.0
43	363417.0
44	369301.5
45	374964.0
46	377461.5
47	377094.0
48	378115.0
49	373378.0
50	363831.0
51	352833.5
52	337001.5
53	316993.0
54	296315.5
55	274555.0
56	250475.5
57	226440.0
58	202543.0
59	176011.5
60	146896.0
61	118890.5
62	93832.5
63	72116.0
64	53709.5
65	40061.5
66	30392.5
67	23250.0
68	17702.5
69	12818.0
70	9040.5
71	6444.0
72	4528.5
73	3128.0
74	2203.5
75	1504.0
76	1002.5
77	661.0
78	419.0
79	269.0
80	191.0
81	141.0
82	101.5
83	72.5
84	50.0
85	31.5
86	24.0
87	19.5
88	13.0
89	9.0
90	5.5
91	4.0
92	4.5
93	4.0
94	3.0
95	3.0
96	3.0
97	2.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.002022141154917955
4	0.014165744154398651
5	0.009024342707320021
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.001586928784983845
26-27	0.036999824363835476
28-29	0.08419285250649373
30-31	0.08277238784274087
32-33	0.03773887044807269
34-35	0.0014721925558583413
36-37	7.213737231128607
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.15767672803511E-5
56-57	2.9162281388602855E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.010859026952640991
74-75	0.03325783992278649
76-77	0.004268130421889155
78-79	0.0
80-81	0.0
82-83	0.003554462650684759
84-85	0.039739672228026185
86-87	0.04346159612495691
88-89	0.012933355594381751
90-91	6.796445241652369E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	132.0
20-21	514.0
22-23	1091.0
24-25	2523.0
26-27	3790.0
28-29	6863.0
30-31	16325.0
32-33	21268.0
34-35	32983.0
36-37	40857.0
38-39	50098.0
40-41	55476.0
42-43	65204.0
44-45	78824.0
46-47	78222.0
48-49	68698.0
50-51	61251.0
52-53	59419.0
54-55	63930.0
56-57	67439.0
58-59	67202.0
60-61	67097.0
62-63	65752.0
64-65	68674.0
66-67	73481.0
68-69	71318.0
70-71	70714.0
72-73	67984.0
74-75	69128.0
76-77	68330.0
78-79	73352.0
80-81	74525.0
82-83	72242.0
84-85	73015.0
86-87	76919.0
88-89	83801.0
90-91	88093.0
92-93	93263.0
94-95	114442.0
96-97	317057.0
98-99	572941.0
100-101	6192839.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.70716381702583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.32927889887269	80.10232025674269
2	6.285303223083651	11.025322388533565
3	1.407427710264216	3.703244782342955
4	0.4636010306524509	1.6264452616470613
5	0.2044878143699969	0.8967523116767444
6	0.10132614103391294	0.533221707036508
7	0.06340292329925962	0.3892623406200541
8	0.03657816652996642	0.2566533795176173
9	0.02484651909093093	0.19612959481720255
>10	0.08169226975888322	1.1010775495410126
>50	0.0015631717731041676	0.09072049348362685
>100	4.740251090606146E-4	0.06676494886462091
>500	1.8106161833408737E-5	0.012084985176374947
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.151213994593569E-5	0.0	0.0	0.0
3	0.0	2.151213994593569E-5	0.0	0.0	0.0
4	0.0	8.604855978374276E-5	0.0	2.151213994593569E-5	0.0
5	0.0	8.604855978374276E-5	0.0	2.151213994593569E-5	0.0
6	0.0	8.604855978374276E-5	0.0	2.151213994593569E-5	0.0
7	2.151213994593569E-5	9.680462975671061E-5	0.0	4.302427989187138E-5	0.0
8	3.226820991890353E-5	1.0756069972967845E-4	0.0	5.3780349864839225E-5	0.0
9	4.302427989187138E-5	2.0436532948638905E-4	0.0	5.3780349864839225E-5	1.0756069972967845E-5
10-11	4.302427989187138E-5	2.0436532948638905E-4	0.0	5.3780349864839225E-5	1.0756069972967845E-5
12-13	4.302427989187138E-5	2.2587746943232475E-4	0.0	5.3780349864839225E-5	1.6134104959451766E-5
14-15	6.453641983780706E-5	2.689017493241961E-4	0.0	5.9158384851323144E-5	2.151213994593569E-5
16-17	6.991445482429099E-5	2.9579192425661574E-4	0.0	6.991445482429099E-5	2.151213994593569E-5
18-19	8.604855978374276E-5	3.119260292160675E-4	0.0	8.067052479725884E-5	3.226820991890353E-5
20-21	1.0218266474319452E-4	3.388162041484871E-4	0.0	8.604855978374276E-5	4.302427989187138E-5
22-23	1.1831676970264629E-4	4.1948672894574594E-4	0.0	1.0218266474319452E-4	4.302427989187138E-5
24-25	1.5058497962154983E-4	4.356208339051977E-4	0.0	1.1831676970264629E-4	4.302427989187138E-5
26-27	1.720971195674855E-4	4.4099886889168165E-4	0.0	1.5058497962154983E-4	4.302427989187138E-5
28-29	1.720971195674855E-4	4.4099886889168165E-4	0.0	3.549503091079389E-4	4.302427989187138E-5
30-31	1.8285318954045337E-4	4.947792187565209E-4	0.0	7.905711430131366E-4	4.302427989187138E-5
32-33	1.936092595134212E-4	4.947792187565209E-4	0.0	0.0017747515455396943	4.302427989187138E-5
34-35	2.3125550441880865E-4	5.378034986483922E-4	0.0	0.0031622845720525464	4.302427989187138E-5
36-37	2.5814567935122826E-4	5.808277785402636E-4	0.0	0.005189803761956985	4.302427989187138E-5
38-39	2.5814567935122826E-4	5.915838485132315E-4	0.0	0.009035098777292989	4.302427989187138E-5
40-41	2.5814567935122826E-4	6.23852058432135E-4	0.0	0.01302560073726406	4.302427989187138E-5
42-43	2.5814567935122826E-4	6.346081284051029E-4	0.0	0.01687627378758655	4.302427989187138E-5
44-45	2.5814567935122826E-4	7.099006182158779E-4	0.0	0.021340042826368207	4.302427989187138E-5
46-47	2.850358542836479E-4	7.744370380536849E-4	0.0	0.026384639643690123	4.302427989187138E-5
48-49	2.904138892701318E-4	7.744370380536849E-4	0.0	0.031439992530985006	4.302427989187138E-5
50-51	2.904138892701318E-4	9.088879127157829E-4	0.0	0.036629796292941996	4.302427989187138E-5
52-53	2.9579192425661574E-4	9.142659477022669E-4	0.0	0.041588344550480175	4.302427989187138E-5
54-55	3.0116995924309967E-4	9.142659477022669E-4	0.0	0.04695024543200464	4.302427989187138E-5
56-57	3.065479942295836E-4	9.142659477022669E-4	0.0	0.052634828412718146	4.84023148783553E-5
58-59	3.119260292160675E-4	9.142659477022669E-4	0.0	0.05830865532345869	5.3780349864839225E-5
60-61	3.226820991890354E-4	9.304000526617186E-4	0.0	0.06417609149371264	5.3780349864839225E-5
62-63	3.2806013417551925E-4	9.465341576211703E-4	0.0	0.06946269988542633	5.3780349864839225E-5
64-65	3.334381691620032E-4	0.001043338787377881	0.0	0.07415234639364032	5.3780349864839225E-5
66-67	3.334381691620032E-4	0.001043338787377881	0.0	0.07912165072115146	5.3780349864839225E-5
68-69	3.334381691620032E-4	0.0010756069972967844	0.0	0.08452657588256782	5.9158384851323144E-5
70-71	3.334381691620032E-4	0.0010917411022562363	0.0	0.08909252758609265	6.453641983780706E-5
72-73	3.4957227412145495E-4	0.0010971191372427201	0.0	0.09279799369178007	6.453641983780706E-5
74-75	3.549503091079389E-4	0.0011508994871075595	0.0	0.09598179040377856	6.453641983780706E-5
76-77	3.549503091079389E-4	0.0011939237669994307	0.0	0.09931079406041211	6.453641983780706E-5
78-79	3.7108441406739066E-4	0.0011939237669994307	0.0	0.10124688665554632	6.453641983780706E-5
80-81	3.764624490538746E-4	0.0012046798369723987	0.0	0.10194065316880274	6.991445482429099E-5
82-83	3.872185190268424E-4	0.0012100578719588825	0.0	0.10234400579278904	7.529248981077492E-5
84-85	3.9797458899981024E-4	0.0012261919769183342	0.0	0.10251610291235652	7.529248981077492E-5
86-87	4.087306589727781E-4	0.00124770411686427	0.0	0.10262366361208622	7.529248981077492E-5
88	4.087306589727781E-4	0.0012907283967561415	0.0	0.10263441968205918	7.529248981077492E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12135	0.0	52.06752	1
GTATCAA	22530	0.0	38.0696	1
TCAACGC	27520	0.0	30.62744	4
ATCAACG	27545	0.0	30.615356	3
CAACGCA	28105	0.0	30.000978	5
TATCAAC	28660	0.0	29.616505	2
AACGCAG	29005	0.0	29.068043	6
ACGCAGA	32950	0.0	25.613533	7
CGCAGAG	34075	0.0	24.780327	8
GTGGTAT	5835	0.0	22.295979	1
GCAGAGT	39315	0.0	21.380556	9
GTACATG	28710	0.0	20.369242	1
TGGTATC	5735	0.0	20.320215	2
TACATGG	28250	0.0	20.205898	2
GAGTACT	22915	0.0	19.801823	12-13
ACATGGG	28715	0.0	19.273624	3
CAGAGTA	38015	0.0	19.14156	10-11
AGAGTAC	35285	0.0	18.929512	10-11
CATGGGG	21500	0.0	17.7981	4
GTACTTT	25825	0.0	17.554636	14-15
>>END_MODULE