FastQCFastQC Report
Fri 27 May 2016
SRR2138925_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138925_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6583224
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82700.053.559461
GTATCAA158700.039.3744431
TCAACGC192700.031.3899484
ATCAACG195350.030.9863623
CAACGCA197650.030.556855
TATCAAC202050.030.400662
AACGCAG204450.029.6621076
ACGCAGA229900.026.3230487
CGCAGAG237950.025.3968018
GCAGAGT276950.021.743719
GTACATG196000.021.2324731
TACATGG199050.020.4800222
GAGTACT166150.020.27048112-13
ACATGGG202400.019.468983
GTGGTAT39150.019.435481
CAGAGTA268700.019.37463610-11
TGGTATC37750.019.1428242
AGAGTAC250250.019.02842910-11
GTACTTT185750.017.98293314-15
CATGGGG155950.017.8534984
Produced by FastQC (version 0.11.5)