##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138925_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6583224
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73043025727212	33.0	33.0	33.0	33.0	33.0
2	32.725167182523336	33.0	33.0	33.0	33.0	33.0
3	32.724446562960644	33.0	33.0	33.0	33.0	33.0
4	36.65548551894938	37.0	37.0	37.0	37.0	37.0
5	36.67375832874592	37.0	37.0	37.0	37.0	37.0
6	36.587932447688246	37.0	37.0	37.0	37.0	37.0
7	36.64172478408755	37.0	37.0	37.0	37.0	37.0
8	36.64617883274213	37.0	37.0	37.0	37.0	37.0
9	36.65512399395798	37.0	37.0	37.0	37.0	37.0
10-11	36.617464634349375	37.0	37.0	37.0	37.0	37.0
12-13	36.592406167555595	37.0	37.0	37.0	37.0	37.0
14-15	38.712438085047694	40.0	37.0	40.0	37.0	40.0
16-17	38.66788757301893	40.0	37.0	40.0	37.0	40.0
18-19	38.69864248884741	40.0	37.0	40.0	37.0	40.0
20-21	38.72071208358209	40.0	37.0	40.0	37.0	40.0
22-23	38.542065181000794	40.0	37.0	40.0	37.0	40.0
24-25	38.718258499670014	40.0	37.0	40.0	37.0	40.0
26-27	38.528966243149355	40.0	37.0	40.0	37.0	40.0
28-29	38.51628821922865	40.0	37.0	40.0	37.0	40.0
30-31	38.49462020219964	40.0	37.0	40.0	37.0	40.0
32-33	38.48803889781015	40.0	37.0	40.0	37.0	40.0
34-35	38.395507916191136	40.0	37.0	40.0	37.0	40.0
36-37	33.177340768157094	40.0	37.0	40.0	8.5	40.0
38-39	37.297830112033694	40.0	37.0	40.0	33.0	40.0
40-41	37.904680484561965	40.0	37.0	40.0	35.0	40.0
42-43	38.12230253519519	40.0	37.0	40.0	37.0	40.0
44-45	38.129888849845706	40.0	37.0	40.0	37.0	40.0
46-47	38.02636078261621	40.0	37.0	40.0	33.0	40.0
48-49	38.006494957301285	40.0	37.0	40.0	33.0	40.0
50-51	37.95984866948038	40.0	37.0	40.0	33.0	40.0
52-53	37.88553204360057	40.0	37.0	40.0	33.0	40.0
54-55	37.79711374384465	40.0	37.0	40.0	33.0	40.0
56-57	37.72155167534817	40.0	37.0	40.0	33.0	40.0
58-59	37.57899496156745	40.0	37.0	40.0	33.0	40.0
60-61	37.38665940026144	38.5	37.0	40.0	33.0	40.0
62-63	37.168526590139464	37.0	37.0	40.0	33.0	40.0
64-65	36.96205449539496	37.0	37.0	40.0	33.0	40.0
66-67	36.69487071816994	37.0	37.0	40.0	33.0	40.0
68-69	36.42327898791185	37.0	37.0	40.0	33.0	40.0
70-71	36.15837067583065	37.0	37.0	38.5	33.0	40.0
72-73	35.90190904109299	37.0	37.0	37.0	33.0	40.0
74-75	35.62087755764193	37.0	35.0	37.0	33.0	40.0
76-77	33.80578859119572	35.0	33.0	37.0	27.0	37.0
78-79	35.23791826398167	37.0	33.0	37.0	33.0	37.0
80-81	35.26849180072364	37.0	33.0	37.0	33.0	37.0
82-83	35.33295549289888	37.0	33.0	37.0	33.0	37.0
84-85	35.191579261595464	37.0	33.0	37.0	33.0	37.0
86-87	35.10730631429646	37.0	33.0	37.0	33.0	37.0
88-89	35.013528338921354	37.0	33.0	37.0	33.0	37.0
90-91	34.87077756086818	37.0	33.0	37.0	33.0	37.0
92-93	34.864620980882364	37.0	33.0	37.0	33.0	37.0
94-95	34.82984718436602	37.0	33.0	37.0	33.0	37.0
96-97	34.80676581938602	37.0	33.0	37.0	33.0	37.0
98-99	34.76496764460522	37.0	33.0	37.0	33.0	37.0
100	34.709934686404964	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	4.0
20	26.0
21	175.0
22	645.0
23	1947.0
24	4599.0
25	9446.0
26	17292.0
27	28101.0
28	42125.0
29	59830.0
30	82689.0
31	111764.0
32	149986.0
33	205574.0
34	292917.0
35	458329.0
36	895172.0
37	2072963.0
38	2081536.0
39	68100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.95567399802893	20.04414554327788	14.252697462519883	23.747482996173304
2	14.574697746879037	20.865900962810926	41.98091998692434	22.578481303385697
3	18.059491548080544	26.400946663588087	30.449209493370926	25.090352294960443
4	12.354544132823625	18.027450187418637	39.138705286872124	30.479300392885612
5	13.212491000933213	37.86249775355645	35.58150871984695	13.343502525663386
6	29.70231606884408	38.200507836282036	18.805892067473323	13.291284027400557
7	26.25516008569661	32.85350764306364	23.277697978984158	17.613634292255586
8	24.309304984913165	36.915149780715346	21.06859800000729	17.706947234364197
9	25.61112913672693	16.930655861018856	21.252930175245442	36.20528482700877
10-11	24.185011781461483	26.784649891907065	28.936027393265064	20.094310933366387
12-13	25.208446499769714	24.364657802924526	28.949014950729307	21.47788074657645
14-15	22.23147807214216	25.64066633612953	26.78512078580343	25.342734805924877
16-17	21.70384146126579	28.767842321634507	27.32594242577801	22.2023737913217
18-19	21.68199806052475	27.86276146763349	29.185737869469424	21.269502602372334
20-21	22.5600368581216	27.019445757996902	29.13329640027215	21.287220983609345
22-23	22.30012377201188	27.001925890985685	28.913772418248378	21.78417791875406
24-25	22.01181414494526	27.139509499358972	29.032293353502688	21.81638300219308
26-27	21.967896625812458	27.24643993701405	28.880486708224012	21.905176728949478
28-29	21.897675936009687	27.29472274520539	29.01229448765005	21.795306831134877
30-31	22.21110456608712	27.179072237137852	28.854927533638232	21.754895663136796
32-33	21.948827874885872	27.40104984730908	28.798817701592128	21.851304576212918
34-35	22.14446667268862	27.40252650003584	28.762639798359306	21.69036702891623
36-37	22.28727727882472	27.28770115343762	28.5556264783666	21.869395089371054
38-39	21.95572588520984	27.43533335455432	28.738805320773796	21.870135439462043
40-41	22.267412279359082	27.335018466585577	28.573649532842467	21.823919721212878
42-43	22.098564645084906	27.398980821752033	28.47693562228681	22.02551891087625
44-45	22.189888706246943	27.574966662935275	28.232972566394277	22.002172064423508
46-47	22.30304737110566	27.535320380513095	27.894140406065215	22.267491842316023
48-49	22.21990762936683	27.64959088439288	27.863617476907343	22.266884009332948
50-51	22.206631803847007	27.786545319558986	27.76331131147603	22.243511565117977
52-53	22.41797614973036	27.71015641988385	27.63139413698364	22.240473293402147
54-55	22.421713262394423	27.57822554820603	27.655658693687908	22.344402495711645
56-57	22.331901274388915	27.787559331717514	27.61755643560321	22.262982958290365
58-59	22.332968301888293	27.78743907585433	27.663313478537678	22.216279143719703
60-61	22.411837940610006	27.80067335301661	27.59732548227729	22.190163224096096
62-63	22.380259795740823	27.864722550544453	27.557707275046013	22.19731037866871
64-65	22.360863517195806	27.80171597517319	27.524447507223186	22.312973000407816
66-67	22.456174249257867	27.791732611155755	27.385678565375933	22.366414574210445
68-69	22.351490715884236	27.781097667972638	27.45553402628061	22.411877589862517
70-71	22.398972156281523	27.59766987106167	27.51448945653059	22.488868516126217
72-73	22.44749981796099	27.660238360259186	27.425256885302456	22.467004936477366
74-75	22.276870095298413	27.791809864043195	27.437241732251184	22.49407830840721
76-77	22.449374385730376	27.72460057722975	27.359051422967973	22.4669736140719
78-79	22.39475256106817	27.731556042593898	27.320590494069457	22.553100902268472
80-81	22.442322873705365	27.774089004750103	27.260790069011225	22.522798052533304
82-83	22.423083686478865	27.75427438909132	27.441086651931506	22.381555272498307
84-85	22.54809969183393	27.717408168949774	27.34629376922344	22.388198369992857
86-87	22.38475791744711	27.777793832271573	27.38542303958861	22.452025210692703
88-89	22.42059224543372	27.721226057895453	27.391749001417097	22.466432695253722
90-91	22.41628159998051	27.83970047447017	27.36154752319417	22.382470402355157
92-93	22.46678508594898	27.73362517061928	27.404817163914675	22.39477257951706
94-95	22.330420053004264	27.80745600886871	27.43931175422815	22.422812183898873
96-97	22.412056745905314	27.89454143537478	27.520992912227577	22.172408906492326
98-99	23.54153611312828	29.809797003205237	27.662683621375102	18.985983262291384
100	22.428003683222975	30.882836605028473	21.559599501384366	25.12956021036419
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1880.0
1	1580.0
2	1916.5
3	2728.5
4	3654.0
5	5788.0
6	7592.5
7	8839.0
8	9759.0
9	9846.5
10	9058.0
11	8112.5
12	8769.0
13	11613.5
14	18070.0
15	26807.0
16	34343.0
17	39332.0
18	40863.5
19	38852.0
20	35011.0
21	31325.0
22	29525.5
23	30958.0
24	35715.0
25	43965.5
26	54873.5
27	66935.0
28	79334.5
29	93045.5
30	107060.0
31	120946.0
32	136227.5
33	151200.5
34	165976.0
35	179869.5
36	195287.5
37	208604.0
38	217747.0
39	226843.0
40	235772.5
41	243499.5
42	247966.5
43	252366.0
44	257202.0
45	260334.0
46	262800.5
47	264488.0
48	263796.5
49	261427.5
50	257141.5
51	250309.5
52	241823.5
53	230387.5
54	217728.5
55	204158.0
56	188678.0
57	171568.0
58	153480.0
59	133925.0
60	112386.0
61	91577.0
62	72488.0
63	55834.0
64	41800.0
65	31123.5
66	23702.0
67	18436.0
68	14076.0
69	10157.5
70	7316.5
71	5360.5
72	3777.0
73	2525.5
74	1648.5
75	1071.0
76	693.5
77	432.5
78	285.0
79	188.5
80	129.5
81	95.0
82	68.0
83	46.0
84	33.0
85	28.0
86	24.5
87	19.5
88	14.5
89	8.5
90	4.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.0015797730716743043
4	0.013686303245947579
5	0.009068505036438074
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0016712976806034661
26-27	0.033534127757047866
28-29	0.07744529508998957
30-31	0.07505443977107597
32-33	0.03390964482391533
34-35	0.0014137635922861695
36-37	7.7025751449931015
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	8.158585940830998E-6
56-57	1.6436514374142733E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.011792809277594057
74-75	0.03334367649761909
76-77	0.00472021798501019
78-79	0.0
80-81	0.0
82-83	0.003494888679561525
84-85	0.03560816615174692
86-87	0.038671190792193784
88-89	0.011708181298740364
90-91	1.903247463565896E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	83.0
20-21	343.0
22-23	651.0
24-25	1739.0
26-27	2530.0
28-29	4692.0
30-31	11291.0
32-33	14627.0
34-35	22813.0
36-37	28620.0
38-39	35201.0
40-41	37938.0
42-43	44562.0
44-45	54517.0
46-47	53768.0
48-49	48219.0
50-51	42429.0
52-53	40227.0
54-55	43410.0
56-57	46233.0
58-59	46387.0
60-61	46228.0
62-63	44539.0
64-65	46294.0
66-67	49449.0
68-69	49019.0
70-71	49018.0
72-73	46108.0
74-75	46789.0
76-77	46615.0
78-79	50117.0
80-81	51309.0
82-83	49359.0
84-85	49412.0
86-87	52412.0
88-89	57133.0
90-91	60528.0
92-93	65092.0
94-95	79244.0
96-97	223692.0
98-99	407513.0
100-101	4433074.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.70323380161507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.2373921338531	80.01814333764953
2	6.364427786992072	11.163617964321224
3	1.3643761944749815	3.589806131321914
4	0.5128095047268976	1.7990020755501406
5	0.2122598195820417	0.9307936291746218
6	0.10938069666311194	0.5755824487697067
7	0.06607543244131255	0.40565203699602786
8	0.03595817231452734	0.25229183948638023
9	0.022412567326575875	0.17690891691034105
>10	0.07376091566145739	0.9671288782843055
>50	9.127054171116807E-4	0.05485784616158451
>100	1.836926018435177E-4	0.03155326696443446
>500	5.037794492976481E-5	0.03466162840970443
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5190125689176003E-5	0.0
2	0.0	3.0380251378352006E-5	0.0	1.5190125689176003E-5	0.0
3	0.0	3.0380251378352006E-5	0.0	1.5190125689176003E-5	0.0
4	0.0	6.076050275670401E-5	0.0	1.5190125689176003E-5	0.0
5	0.0	7.595062844588001E-5	0.0	1.5190125689176003E-5	0.0
6	0.0	7.595062844588001E-5	0.0	1.5190125689176003E-5	0.0
7	0.0	7.595062844588001E-5	0.0	1.5190125689176003E-5	0.0
8	0.0	1.0633087982423202E-4	0.0	1.5190125689176003E-5	1.5190125689176003E-5
9	0.0	2.7342226240516807E-4	0.0	3.0380251378352006E-5	1.5190125689176003E-5
10-11	0.0	2.7342226240516807E-4	0.0	3.0380251378352006E-5	1.5190125689176003E-5
12-13	0.0	2.7342226240516807E-4	0.0	3.0380251378352006E-5	1.5190125689176003E-5
14-15	0.0	3.7975314222940006E-4	0.0	3.0380251378352006E-5	1.5190125689176003E-5
16-17	0.0	3.7975314222940006E-4	0.0	4.5570377067528005E-5	1.5190125689176003E-5
18-19	0.0	4.101333936077521E-4	0.0	6.0760502756704006E-5	3.797531422294001E-5
20-21	0.0	4.405136449861041E-4	0.0	7.595062844588001E-5	4.5570377067528005E-5
22-23	7.595062844588002E-6	4.6329883351986804E-4	0.0	7.595062844588001E-5	5.316543991211601E-5
24-25	2.2785188533764006E-5	5.088692105873961E-4	0.0	7.595062844588001E-5	6.835556560129202E-5
26-27	3.0380251378352006E-5	5.392494619657481E-4	0.0	1.2911606835799602E-4	7.595062844588001E-5
28-29	3.0380251378352006E-5	5.468445248103361E-4	0.0	2.2785188533764006E-4	7.595062844588001E-5
30-31	3.0380251378352006E-5	6.076050275670401E-4	0.0	8.202667872155042E-4	7.595062844588001E-5
32-33	4.5570377067528005E-5	6.076050275670401E-4	0.0	0.002103832407950876	1.0633087982423202E-4
34-35	4.5570377067528005E-5	6.759605931683321E-4	0.0	0.003729175856692709	1.2152100551340803E-4
36-37	4.5570377067528005E-5	7.291260330804481E-4	0.0	0.006911507188575081	1.2152100551340803E-4
38-39	4.5570377067528005E-5	7.443161587696242E-4	0.0	0.012379952436678443	1.2152100551340803E-4
40-41	4.5570377067528005E-5	8.202667872155041E-4	0.0	0.01819777057563285	1.4430619404717202E-4
42-43	4.5570377067528005E-5	8.354569129046801E-4	0.0	0.023613050383824094	1.5190125689176003E-4
44-45	4.5570377067528005E-5	9.417877927289122E-4	0.0	0.029590364842514854	1.5190125689176003E-4
46-47	4.5570377067528005E-5	0.0010329285468639682	0.0	0.03678288935633969	1.5190125689176003E-4
48-49	4.5570377067528005E-5	0.0010329285468639682	0.0	0.04410452993852253	1.5190125689176003E-4
50-51	4.5570377067528005E-5	0.0011924248666003163	0.0	0.05100844206425302	1.5190125689176003E-4
52-53	4.5570377067528005E-5	0.0012000199294449042	0.0	0.05794273444136186	1.5949631973634804E-4
54-55	4.5570377067528005E-5	0.0012000199294449042	0.0	0.06384409827160674	1.74686445425524E-4
56-57	4.5570377067528005E-5	0.0012000199294449042	0.0	0.06854544217240671	1.8987657111470003E-4
58-59	4.5570377067528005E-5	0.0012000199294449042	0.0	0.07347463795854432	1.9747163395928804E-4
60-61	4.5570377067528005E-5	0.0012152100551340802	0.0	0.07841142880752652	2.1266175964846404E-4
62-63	4.5570377067528005E-5	0.0012304001808232563	0.0	0.08325707890237367	2.1266175964846404E-4
64-65	4.5570377067528005E-5	0.0013367310606474882	0.0	0.08778373635774811	2.1266175964846404E-4
66-67	4.5570377067528005E-5	0.0013443261234920762	0.0	0.09234836912734551	2.1266175964846404E-4
68-69	4.5570377067528005E-5	0.0013747063748704282	0.0	0.09700414265107796	2.1266175964846404E-4
70-71	4.5570377067528005E-5	0.0013898965005596042	0.0	0.10143965935231734	2.2025682249305202E-4
72-73	4.5570377067528005E-5	0.0013974915634041923	0.0	0.10544225747141522	2.4304201102681605E-4
74-75	4.5570377067528005E-5	0.0014354668776271323	0.0	0.10815369490693313	2.4304201102681605E-4
76-77	4.5570377067528005E-5	0.0014810372546946603	0.0	0.11081196690253894	2.4304201102681605E-4
78-79	4.5570377067528005E-5	0.0014886323175392483	0.0	0.11226262390585524	2.4304201102681605E-4
80-81	4.5570377067528005E-5	0.0014886323175392483	0.0	0.1127942783049764	2.5063707387140406E-4
82-83	4.5570377067528005E-5	0.0014886323175392483	0.0	0.11324998207565168	2.7342226240516807E-4
84-85	4.5570377067528005E-5	0.0015114175060730122	0.0	0.11339428826969886	2.7342226240516807E-4
86-87	4.5570377067528005E-5	0.0015645829459851282	0.0	0.11347023889814474	2.7342226240516807E-4
88	4.5570377067528005E-5	0.0017164842028768884	0.0	0.11347023889814474	2.7342226240516807E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8270	0.0	53.55946	1
GTATCAA	15870	0.0	39.374443	1
TCAACGC	19270	0.0	31.389948	4
ATCAACG	19535	0.0	30.986362	3
CAACGCA	19765	0.0	30.55685	5
TATCAAC	20205	0.0	30.40066	2
AACGCAG	20445	0.0	29.662107	6
ACGCAGA	22990	0.0	26.323048	7
CGCAGAG	23795	0.0	25.396801	8
GCAGAGT	27695	0.0	21.74371	9
GTACATG	19600	0.0	21.232473	1
TACATGG	19905	0.0	20.480022	2
GAGTACT	16615	0.0	20.270481	12-13
ACATGGG	20240	0.0	19.46898	3
GTGGTAT	3915	0.0	19.43548	1
CAGAGTA	26870	0.0	19.374636	10-11
TGGTATC	3775	0.0	19.142824	2
AGAGTAC	25025	0.0	19.028429	10-11
GTACTTT	18575	0.0	17.982933	14-15
CATGGGG	15595	0.0	17.853498	4
>>END_MODULE