FastQCFastQC Report
Fri 27 May 2016
SRR2138924_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138924_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10030354
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130550.051.960721
GTATCAA246750.038.8303571
TCAACGC299700.031.1516914
ATCAACG300600.031.0727923
CAACGCA303950.030.6843225
TATCAAC310400.030.364082
AACGCAG315350.029.6133586
ACGCAGA358700.026.0698327
CGCAGAG367350.025.4559678
GCAGAGT424700.021.9687359
GTACATG303600.021.2854421
TACATGG303200.020.8953382
GAGTACT257650.020.09885812-13
ACATGGG311250.019.7981743
GTGGTAT60900.019.570811
CAGAGTA415050.019.25215310-11
AGAGTAC386350.019.0635910-11
CTTATAC59500.019.0368391
CATGGGG235100.018.2108174
GTACTTT286700.017.99663714-15
Produced by FastQC (version 0.11.5)