FastQCFastQC Report
Fri 27 May 2016
SRR2138923_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138923_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7879987
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99200.048.2456361
GTATCAA184850.036.5507741
TCAACGC207450.031.4221024
ATCAACG208400.031.360773
CAACGCA214550.030.4364285
TATCAAC219600.030.090062
AACGCAG219650.029.805356
ACGCAGA251450.025.9853217
CGCAGAG257050.025.435738
GCAGAGT301650.021.6186689
GTACATG216050.021.3855511
TACATGG219250.020.6085682
GAGTACT182500.019.9020712-13
ACATGGG219600.019.8021983
GTGGTAT48150.019.0503641
CAGAGTA297300.018.90750110-11
AGAGTAC276800.018.57458510-11
CATGGGG180150.017.5379564
GTACTTT207250.017.48476214-15
TGGTATC52650.016.2929152
Produced by FastQC (version 0.11.5)