##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138923_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7879987
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71154508249823	33.0	33.0	33.0	33.0	33.0
2	32.70261803731402	33.0	33.0	33.0	33.0	33.0
3	32.70470217780816	33.0	33.0	33.0	33.0	33.0
4	36.62935459157483	37.0	37.0	37.0	37.0	37.0
5	36.648108048909215	37.0	37.0	37.0	37.0	37.0
6	36.57191997905581	37.0	37.0	37.0	37.0	37.0
7	36.620810161235035	37.0	37.0	37.0	37.0	37.0
8	36.62618440867986	37.0	37.0	37.0	37.0	37.0
9	36.6334229993019	37.0	37.0	37.0	37.0	37.0
10-11	36.592365507709594	37.0	37.0	37.0	37.0	37.0
12-13	36.56426076591243	37.0	37.0	37.0	37.0	37.0
14-15	38.66921963196132	40.0	37.0	40.0	37.0	40.0
16-17	38.61738299822068	40.0	37.0	40.0	37.0	40.0
18-19	38.64510347542451	40.0	37.0	40.0	37.0	40.0
20-21	38.66755728073191	40.0	37.0	40.0	37.0	40.0
22-23	38.52047993238931	40.0	37.0	40.0	37.0	40.0
24-25	38.67408201975678	40.0	37.0	40.0	37.0	40.0
26-27	38.46279120592735	40.0	37.0	40.0	37.0	40.0
28-29	38.45407713856329	40.0	37.0	40.0	37.0	40.0
30-31	38.43818732748392	40.0	37.0	40.0	37.0	40.0
32-33	38.4377149491225	40.0	37.0	40.0	37.0	40.0
34-35	38.3518139849766	40.0	37.0	40.0	37.0	40.0
36-37	33.30597242558363	40.0	37.0	40.0	8.5	40.0
38-39	37.28064093306547	40.0	37.0	40.0	33.0	40.0
40-41	37.85463546180533	40.0	37.0	40.0	35.0	40.0
42-43	38.067915665714736	40.0	37.0	40.0	35.0	40.0
44-45	38.07720994664917	40.0	37.0	40.0	35.0	40.0
46-47	37.97494610417693	40.0	37.0	40.0	33.0	40.0
48-49	37.955434499245996	40.0	37.0	40.0	33.0	40.0
50-51	37.913534247269055	40.0	37.0	40.0	33.0	40.0
52-53	37.841077805324815	40.0	37.0	40.0	33.0	40.0
54-55	37.75323367413354	40.0	37.0	40.0	33.0	40.0
56-57	37.67622076837787	40.0	37.0	40.0	33.0	40.0
58-59	37.53061339194988	40.0	37.0	40.0	33.0	40.0
60-61	37.3384954625804	37.0	37.0	40.0	33.0	40.0
62-63	37.111762545264206	37.0	37.0	40.0	33.0	40.0
64-65	36.895364853921606	37.0	37.0	40.0	33.0	40.0
66-67	36.62229769167093	37.0	37.0	40.0	33.0	40.0
68-69	36.34489948149114	37.0	37.0	40.0	33.0	40.0
70-71	36.0781618812524	37.0	37.0	38.5	33.0	40.0
72-73	35.81842114676462	37.0	37.0	37.0	33.0	40.0
74-75	35.53747372551754	37.0	33.0	37.0	33.0	40.0
76-77	33.69352890491429	35.0	33.0	37.0	27.0	37.0
78-79	35.167508824766344	37.0	33.0	37.0	33.0	37.0
80-81	35.201113391601055	37.0	33.0	37.0	33.0	37.0
82-83	35.277453159265015	37.0	33.0	37.0	33.0	37.0
84-85	35.126416929226124	37.0	33.0	37.0	33.0	37.0
86-87	35.05960063922703	37.0	33.0	37.0	33.0	37.0
88-89	34.97323964908704	37.0	33.0	37.0	33.0	37.0
90-91	34.83763359135517	37.0	33.0	37.0	33.0	37.0
92-93	34.817011383107584	37.0	33.0	37.0	33.0	37.0
94-95	34.777334767981685	37.0	33.0	37.0	33.0	37.0
96-97	34.75618445795328	37.0	33.0	37.0	33.0	37.0
98-99	34.71332582852978	37.0	33.0	37.0	33.0	37.0
100	34.65677879828699	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	8.0
20	42.0
21	234.0
22	913.0
23	2677.0
24	6234.0
25	12543.0
26	22349.0
27	36205.0
28	54019.0
29	76273.0
30	103852.0
31	140453.0
32	187012.0
33	252539.0
34	357193.0
35	553704.0
36	1073238.0
37	2463776.0
38	2454427.0
39	82295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.789282139678654	20.065997570808175	14.174122368475988	23.97059792103718
2	14.540239723745737	21.063042362887145	41.719485070216486	22.67723284315063
3	18.010883722146172	26.370443261482052	30.495361518081392	25.123311498290384
4	12.498273866336332	18.018639705135755	39.04969995735434	30.433386471173566
5	13.354851253736996	37.74047113686402	35.53766795222534	13.367009657173638
6	29.763665853763467	38.087778571208304	18.85326714371483	13.295288431313402
7	26.307073856847733	32.756589573053866	23.398921851013206	17.537414719085195
8	24.328898004527165	36.78405814628882	21.172230360278512	17.714813488905502
9	25.64771997720301	16.938390888208318	21.28113916939203	36.132749965196645
10-11	24.27990680695285	26.631046472538593	29.0139184239771	20.075128296531453
12-13	25.137687409890397	24.290649210461897	29.054959100820852	21.516704278826857
14-15	22.280944118308824	25.51505478371982	26.69850216757972	25.505498930391635
16-17	21.79246615508376	28.710561324530104	27.271961235469043	22.225011284917095
18-19	21.84247892794747	27.868459681469016	29.039775065618763	21.24928632496475
20-21	22.643895397257232	27.04376531801994	28.87540788565718	21.43693139906565
22-23	22.521807796944195	26.909200725141297	28.655791662499624	21.913199815414888
24-25	22.230765198023768	27.12360543536363	28.684374110238913	21.961255256373686
26-27	22.17690274534964	27.104336293184485	28.694587577059323	22.024173384406552
28-29	22.092974912927957	27.224109973318335	28.709909799034396	21.973005314719313
30-31	22.279589333942127	27.145155208705958	28.61516835809294	21.960087099258974
32-33	22.09687183466059	27.28624279974388	28.605846656073087	22.011038709522442
34-35	22.263719138450977	27.177585689067595	28.557014438832635	22.001680733648794
36-37	22.487176300897165	27.171759512936543	28.299068150877797	22.041996035288495
38-39	22.200804540823153	27.371822580734467	28.51463829849698	21.9127345799454
40-41	22.42845978710556	27.240680422785392	28.308324415100856	22.022535375008196
42-43	22.24303496097286	27.44157650635127	28.12672327002249	22.188665262653377
44-45	22.38667370362368	27.467693821003376	27.999584919239634	22.14604755613331
46-47	22.40919375014347	27.457689616532143	27.746018028182952	22.387098605141436
48-49	22.309689667126907	27.685655026310485	27.62220813328743	22.38244717327518
50-51	22.37715564778224	27.669696365228425	27.595429189230735	22.3577187977586
52-53	22.55970827438631	27.626749766889404	27.36873683309822	22.444805125626065
54-55	22.544889347322147	27.520921143933236	27.449338997341904	22.484850511402712
56-57	22.506544141638305	27.62418090178121	27.44871228800315	22.420562668577336
58-59	22.429366020930793	27.71846912350894	27.441388406674577	22.410776448885695
60-61	22.5679783334923	27.62453953689672	27.4582004739984	22.34928165561258
62-63	22.580329474772206	27.762257090122745	27.33052339268917	22.326890042415883
64-65	22.559299301961694	27.640470659472182	27.320284138656948	22.479945899909172
66-67	22.625962935114877	27.67646164630427	27.17271902131553	22.524856397265324
68-69	22.438643404317034	27.67685161792304	27.300304034981	22.584200942778924
70-71	22.488943023916043	27.52103554139994	27.326137832217285	22.66388360246673
72-73	22.55682169665836	27.591194920208217	27.204419743894686	22.647563639238736
74-75	22.423343588307468	27.67945724703794	27.28257364845334	22.61462551620125
76-77	22.59288198219721	27.612271306362047	27.224723130936475	22.570123580504273
78-79	22.479032051690947	27.585849107708434	27.17464583587377	22.760473004726848
80-81	22.656911404279345	27.62407674077719	27.081494374245235	22.63751748069823
82-83	22.481588286095988	27.674275887091053	27.28657581711529	22.55756000969767
84-85	22.627778713908704	27.63061791008096	27.166672453579743	22.574930922430596
86-87	22.542035586723948	27.621119755201	27.246180696854683	22.590663961220375
88-89	22.53718978468144	27.609892114695082	27.2191174360233	22.633800664600177
90-91	22.582199727861283	27.640965509999987	27.190310559521606	22.58652420261712
92-93	22.643483519904166	27.584661168751474	27.278074832620934	22.49378047872343
94-95	22.393397046587502	27.698383868303782	27.329958467918186	22.578260617190526
96-97	22.498854517457655	27.74022375509625	27.373711071328717	22.387210656117375
98-99	23.72264538946966	29.683832837343637	27.421201403697932	19.172320369488773
100	22.6273413779474	30.682385498214714	21.321484690685647	25.36878843315224
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2025.0
1	1767.0
2	2175.0
3	3028.5
4	3971.0
5	6273.5
6	8304.0
7	9679.5
8	10670.5
9	10729.5
10	9938.0
11	9119.0
12	9820.5
13	12650.5
14	19165.0
15	28045.0
16	35976.0
17	41572.0
18	43318.5
19	41221.0
20	37626.0
21	33939.0
22	32696.5
23	35247.5
24	40904.0
25	50308.5
26	63582.0
27	77530.0
28	91816.5
29	108317.5
30	124574.0
31	141749.5
32	160855.0
33	179447.5
34	196369.0
35	211665.0
36	229421.5
37	247610.5
38	260783.0
39	269601.0
40	279056.0
41	289048.0
42	296365.0
43	303887.0
44	312535.5
45	317785.5
46	317763.0
47	318350.0
48	319640.5
49	318102.0
50	312682.5
51	304333.0
52	294557.5
53	281117.5
54	264983.0
55	246750.5
56	228688.0
57	209450.5
58	188239.5
59	164563.0
60	137274.0
61	111955.5
62	88949.0
63	68664.0
64	52372.0
65	39930.0
66	30541.0
67	23576.5
68	18244.5
69	13607.5
70	10002.0
71	7392.5
72	5321.0
73	3681.0
74	2536.0
75	1698.5
76	1121.5
77	732.0
78	456.5
79	316.0
80	225.0
81	152.5
82	105.5
83	69.0
84	43.5
85	32.5
86	28.5
87	23.0
88	16.0
89	10.0
90	7.0
91	5.0
92	3.5
93	2.5
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.0018527949348139787
4	0.01404824652629503
5	0.008413719464257998
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0015422816514751925
26-27	0.03533094653161209
28-29	0.08249045852740906
30-31	0.07945288199959577
32-33	0.036413725140005265
34-35	0.0013535583900827586
36-37	7.427002546553949
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	3.405105233415197E-5
56-57	7.546441314378777E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.011045943218142226
74-75	0.03365003841921894
76-77	0.004549983881440079
78-79	0.0
80-81	0.0
82-83	0.0032314314444927376
84-85	0.037862229565855963
86-87	0.04148909231077149
88-89	0.012274055037777822
90-91	1.5957468877350574E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	99.0
20-21	415.0
22-23	909.0
24-25	2282.0
26-27	3293.0
28-29	5517.0
30-31	13425.0
32-33	17474.0
34-35	27063.0
36-37	33277.0
38-39	40931.0
40-41	45233.0
42-43	51981.0
44-45	63666.0
46-47	63082.0
48-49	56389.0
50-51	50944.0
52-53	49139.0
54-55	52942.0
56-57	55401.0
58-59	55959.0
60-61	55109.0
62-63	53292.0
64-65	56263.0
66-67	60252.0
68-69	60681.0
70-71	60087.0
72-73	57697.0
74-75	58728.0
76-77	58149.0
78-79	62311.0
80-81	63015.0
82-83	61430.0
84-85	62135.0
86-87	65601.0
88-89	70732.0
90-91	74967.0
92-93	80393.0
94-95	97213.0
96-97	275042.0
98-99	487442.0
100-101	5270027.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.80521279969612
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.04752600657142	81.74300134699138
2	5.806347437330625	10.312678395222326
3	1.236690429601096	3.2947367030421884
4	0.44520621835704494	1.581465318437814
5	0.18224625398117328	0.8092208683372779
6	0.0916691012424587	0.4884416425796059
7	0.060515022566260325	0.3761834619602613
8	0.03596417529049327	0.2555044991870262
9	0.023687905000594985	0.18932484999211407
>10	0.06936999309040362	0.8884634059926328
>50	6.726374005816558E-4	0.03946772420897306
>100	1.048195677643317E-4	0.02151178404830367
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.269037626584917E-5	0.0
2	0.0	1.269037626584917E-5	0.0	1.269037626584917E-5	0.0
3	0.0	1.269037626584917E-5	0.0	1.269037626584917E-5	0.0
4	0.0	1.269037626584917E-5	0.0	1.269037626584917E-5	0.0
5	0.0	1.269037626584917E-5	0.0	1.269037626584917E-5	0.0
6	0.0	1.269037626584917E-5	0.0	1.269037626584917E-5	1.269037626584917E-5
7	0.0	1.269037626584917E-5	0.0	1.269037626584917E-5	1.269037626584917E-5
8	0.0	1.269037626584917E-5	0.0	1.269037626584917E-5	2.538075253169834E-5
9	0.0	2.538075253169834E-5	0.0	1.269037626584917E-5	5.076150506339668E-5
10-11	0.0	2.538075253169834E-5	0.0	1.269037626584917E-5	5.076150506339668E-5
12-13	0.0	2.538075253169834E-5	0.0	1.269037626584917E-5	5.076150506339668E-5
14-15	0.0	5.076150506339668E-5	0.0	1.269037626584917E-5	8.248744572801961E-5
16-17	0.0	5.076150506339668E-5	0.0	1.269037626584917E-5	1.0152301012679336E-4
18-19	0.0	5.710669319632126E-5	0.0	1.269037626584917E-5	1.0152301012679336E-4
20-21	0.0	6.345188132924584E-5	0.0	1.269037626584917E-5	1.0152301012679336E-4
22-23	0.0	8.883263386094418E-5	0.0	1.269037626584917E-5	1.1421338639264252E-4
24-25	0.0	1.0152301012679335E-4	0.0	3.172594066462292E-5	1.2690376265849169E-4
26-27	0.0	1.1421338639264253E-4	0.0	5.710669319632126E-5	1.3959413892434085E-4
28-29	0.0	1.1421338639264253E-4	0.0	2.030460202535867E-4	1.3959413892434085E-4
30-31	0.0	1.5228451519019002E-4	0.0	0.0010723367944642547	1.3959413892434085E-4
32-33	0.0	1.5228451519019002E-4	0.0	0.0030774162444684233	1.649748914560392E-4
34-35	0.0	2.093912083865113E-4	0.0	0.006338842944791661	1.649748914560392E-4
36-37	0.0	2.6649790158283257E-4	0.0	0.011008901410624154	1.649748914560392E-4
38-39	0.0	2.791882778486817E-4	0.0	0.020869323769188958	1.649748914560392E-4
40-41	0.0	3.1725940664622924E-4	0.0	0.028083802676324213	1.7766526772188836E-4
42-43	0.0	3.236045947791538E-4	0.0	0.03430843223472323	1.9035564398773756E-4
44-45	0.0	3.553305354437767E-4	0.0	0.0415800178350548	2.0304602025358672E-4
46-47	0.0	3.807112879754751E-4	0.0	0.04886429381165223	2.157363965194359E-4
48-49	0.0	3.807112879754751E-4	0.0	0.05598994008492654	2.4111714905113423E-4
50-51	0.0	4.568535455705701E-4	0.0	0.06291888552608019	2.4111714905113423E-4
52-53	0.0	4.568535455705701E-4	0.0	0.06988590209603138	2.5380752531698337E-4
54-55	0.0	4.568535455705701E-4	0.0	0.07634530361534861	2.728430897157571E-4
56-57	0.0	4.6954392183641926E-4	0.0	0.08213211519257582	2.982238422474555E-4
58-59	0.0	4.6954392183641926E-4	0.0	0.08817273429512003	3.1725940664622924E-4
60-61	0.0	4.7588910996934386E-4	0.0	0.09404203331807527	3.299497829120784E-4
62-63	0.0	4.949246743681177E-4	0.0	0.09947985954799163	3.299497829120784E-4
64-65	0.0	5.837573082290618E-4	0.0	0.10428316696461555	3.299497829120784E-4
66-67	0.0	5.837573082290618E-4	0.0	0.1101080496706403	3.299497829120784E-4
68-69	0.0	6.091380607607601E-4	0.0	0.11537455582096773	3.299497829120784E-4
70-71	0.0	6.091380607607601E-4	0.0	0.12004461428680022	3.680209117096259E-4
72-73	0.0	6.154832488936847E-4	0.0	0.1237819300970928	3.9340166424132425E-4
74-75	0.0	6.472091895583076E-4	0.0	0.1265991936281113	3.9340166424132425E-4
76-77	0.0	7.043158827546289E-4	0.0	0.12946721866419322	3.9974685237424885E-4
78-79	0.0	7.360418234192518E-4	0.0	0.13143422698539986	4.0609204050717345E-4
80-81	0.0	7.360418234192518E-4	0.0	0.13203067466989477	4.251276049059472E-4
82-83	0.0	7.360418234192518E-4	0.0	0.1324050407697373	4.4416316930472093E-4
84-85	0.0	7.360418234192518E-4	0.0	0.1325890512255921	4.4416316930472093E-4
86-87	0.0	7.48732199685101E-4	0.0	0.13272230017638353	4.4416316930472093E-4
88	0.0	7.48732199685101E-4	0.0	0.13272864536451645	4.4416316930472093E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9920	0.0	48.245636	1
GTATCAA	18485	0.0	36.550774	1
TCAACGC	20745	0.0	31.422102	4
ATCAACG	20840	0.0	31.36077	3
CAACGCA	21455	0.0	30.436428	5
TATCAAC	21960	0.0	30.09006	2
AACGCAG	21965	0.0	29.80535	6
ACGCAGA	25145	0.0	25.985321	7
CGCAGAG	25705	0.0	25.43573	8
GCAGAGT	30165	0.0	21.618668	9
GTACATG	21605	0.0	21.385551	1
TACATGG	21925	0.0	20.608568	2
GAGTACT	18250	0.0	19.90207	12-13
ACATGGG	21960	0.0	19.802198	3
GTGGTAT	4815	0.0	19.050364	1
CAGAGTA	29730	0.0	18.907501	10-11
AGAGTAC	27680	0.0	18.574585	10-11
CATGGGG	18015	0.0	17.537956	4
GTACTTT	20725	0.0	17.484762	14-15
TGGTATC	5265	0.0	16.292915	2
>>END_MODULE