FastQCFastQC Report
Fri 27 May 2016
SRR2138922_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138922_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9481611
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121750.048.1854861
GTATCAA227350.038.1812021
ATCAACG268000.031.7853853
TCAACGC267750.031.7830494
CAACGCA274100.030.9946825
TATCAAC280650.030.5046082
AACGCAG282150.030.1979986
ACGCAGA324150.026.2458067
CGCAGAG326600.026.009778
GCAGAGT385750.022.0104459
GTACATG275600.021.5031641
TACATGG277400.020.887182
ACATGGG279800.020.037563
GAGTACT229150.019.85607512-13
GTGGTAT59700.019.4964311
AGAGTAC349150.019.19658710-11
CAGAGTA378200.018.83780310-11
CATGGGG219050.018.3346314
GTACTTT259500.017.46841614-15
AGTACTT252850.017.20265412-13
Produced by FastQC (version 0.11.5)