##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138922_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9481611
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72443923295313	33.0	33.0	33.0	33.0	33.0
2	32.7181312331839	33.0	33.0	33.0	33.0	33.0
3	32.71703658798067	33.0	33.0	33.0	33.0	33.0
4	36.646753911334265	37.0	37.0	37.0	37.0	37.0
5	36.66385364259302	37.0	37.0	37.0	37.0	37.0
6	36.58438486877388	37.0	37.0	37.0	37.0	37.0
7	36.63400143709756	37.0	37.0	37.0	37.0	37.0
8	36.63850921536435	37.0	37.0	37.0	37.0	37.0
9	36.64819005968501	37.0	37.0	37.0	37.0	37.0
10-11	36.60872213593238	37.0	37.0	37.0	37.0	37.0
12-13	36.580076160053395	37.0	37.0	37.0	37.0	37.0
14-15	38.69013952375815	40.0	37.0	40.0	37.0	40.0
16-17	38.640243730733104	40.0	37.0	40.0	37.0	40.0
18-19	38.67260748199858	40.0	37.0	40.0	37.0	40.0
20-21	38.697339952264784	40.0	37.0	40.0	37.0	40.0
22-23	38.546595673690376	40.0	37.0	40.0	37.0	40.0
24-25	38.697839291397436	40.0	37.0	40.0	37.0	40.0
26-27	38.48789848363991	40.0	37.0	40.0	37.0	40.0
28-29	38.4793497129896	40.0	37.0	40.0	37.0	40.0
30-31	38.46120209870854	40.0	37.0	40.0	37.0	40.0
32-33	38.458172190239395	40.0	37.0	40.0	37.0	40.0
34-35	38.36938805492	40.0	37.0	40.0	37.0	40.0
36-37	33.344628719838695	40.0	37.0	40.0	8.5	40.0
38-39	37.30203138756245	40.0	37.0	40.0	33.0	40.0
40-41	37.87604776113587	40.0	37.0	40.0	35.0	40.0
42-43	38.09053732869032	40.0	37.0	40.0	35.0	40.0
44-45	38.10103050802344	40.0	37.0	40.0	35.0	40.0
46-47	37.99819387088816	40.0	37.0	40.0	33.0	40.0
48-49	37.97946940044919	40.0	37.0	40.0	33.0	40.0
50-51	37.932509285810156	40.0	37.0	40.0	33.0	40.0
52-53	37.862580613417066	40.0	37.0	40.0	33.0	40.0
54-55	37.7779838384796	40.0	37.0	40.0	33.0	40.0
56-57	37.697115109392975	40.0	37.0	40.0	33.0	40.0
58-59	37.55538129793487	40.0	37.0	40.0	33.0	40.0
60-61	37.36752269581099	38.5	37.0	40.0	33.0	40.0
62-63	37.148010129265394	37.0	37.0	40.0	33.0	40.0
64-65	36.94105404435787	37.0	37.0	40.0	33.0	40.0
66-67	36.672036035525494	37.0	37.0	40.0	33.0	40.0
68-69	36.3968368784063	37.0	37.0	40.0	33.0	40.0
70-71	36.13137857387578	37.0	37.0	38.5	33.0	40.0
72-73	35.8758733923503	37.0	37.0	37.0	33.0	40.0
74-75	35.589696077214846	37.0	35.0	37.0	33.0	40.0
76-77	33.74692893176116	35.0	33.0	37.0	27.0	37.0
78-79	35.20536052780745	37.0	33.0	37.0	33.0	37.0
80-81	35.22816634717745	37.0	33.0	37.0	33.0	37.0
82-83	35.30047575609579	37.0	33.0	37.0	33.0	37.0
84-85	35.147610137434185	37.0	33.0	37.0	33.0	37.0
86-87	35.07572536580613	37.0	33.0	37.0	33.0	37.0
88-89	34.99048182207923	37.0	33.0	37.0	33.0	37.0
90-91	34.85361692214603	37.0	33.0	37.0	33.0	37.0
92-93	34.83804360587338	37.0	33.0	37.0	33.0	37.0
94-95	34.79941181477185	37.0	33.0	37.0	33.0	37.0
96-97	34.77659754279901	37.0	33.0	37.0	33.0	37.0
98-99	34.7373736431185	37.0	33.0	37.0	33.0	37.0
100	34.680582889523926	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	11.0
20	43.0
21	253.0
22	1027.0
23	3021.0
24	7089.0
25	14523.0
26	26082.0
27	42224.0
28	63033.0
29	89486.0
30	122727.0
31	164658.0
32	220631.0
33	301359.0
34	424683.0
35	662831.0
36	1282193.0
37	2957154.0
38	2999892.0
39	98689.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.32972761696298	20.418914043193716	14.42058738752307	23.830770952320233
2	14.326753122438793	21.23529429756188	41.970779016350704	22.467173563648622
3	17.728698240298012	26.831560834409558	30.685450039999218	24.754290885293205
4	12.305944877670488	18.469064678993146	39.5309808892043	29.694009554132066
5	13.189265816803331	37.92917008587937	35.68635992549105	13.19520417182625
6	29.09331547138983	38.539821977509945	19.14425723645486	13.222605314645369
7	25.853423010077087	33.04251777466931	23.66819309503417	17.435866120219444
8	24.149514254486924	36.74151998009621	21.492782186487087	17.616183578929785
9	25.66621853606945	16.88816383629322	21.47050749076291	35.97511013687442
10-11	24.002076229450882	26.8876248983427	29.15113265034813	19.95916622185829
12-13	24.97423169965526	24.50723827417092	29.13684182993797	21.381688196235853
14-15	22.129984029085353	25.692791024647605	26.932875647397896	25.244349298869146
16-17	21.51468774662871	28.91989030134225	27.457443677029147	22.107978274999894
18-19	21.595343871415945	28.05097150684625	29.29616602073213	21.057518601005672
20-21	22.464988697610927	27.165305976621074	29.12927702415334	21.24042830161466
22-23	22.270344332208158	27.175018201369777	28.890400725470606	21.66423674095146
24-25	22.00624988493981	27.304910797955152	29.014268729649512	21.67457058745553
26-27	21.924802830187183	27.37978593067161	28.89633996029419	21.79907127884702
28-29	21.85438738804093	27.501550589641464	28.917658049849337	21.72640397246827
30-31	22.119675131917838	27.359884973621725	28.85264201873621	21.667797875724236
32-33	21.981263423319476	27.482566230533873	28.825830040576257	21.710340305570398
34-35	22.086812128887882	27.45209235903488	28.811569688463546	21.649525823613693
36-37	22.293218919148046	27.411682719042002	28.543716439806825	21.75138192200313
38-39	21.969815281047374	27.52768507463979	28.78097591578354	21.721523728529295
40-41	22.18446780794968	27.41213057816458	28.59178624687835	21.811615367007388
42-43	22.017533966713206	27.568455193494568	28.45225625915253	21.9617545806397
44-45	22.158379931534146	27.642272443277243	28.272609916932506	21.926737708256105
46-47	22.20306173845598	27.5991670169594	27.970553151464017	22.22721809312061
48-49	22.126146150419228	27.811487619274207	27.895708965769266	22.1666572645373
50-51	22.13250173173059	27.843028706390292	27.826192668589883	22.198276893289233
52-53	22.272058751502584	27.81520928403603	27.683589349610905	22.229142614850485
54-55	22.25380214236562	27.723773273916585	27.713834946167204	22.308589637550593
56-57	22.310412101106593	27.79304023594818	27.663380502490554	22.23316716045467
58-59	22.14692894869045	27.982568314922457	27.742473372904687	22.128029363482405
60-61	22.309871873769783	27.89055717262269	27.659725935202335	22.1398450184052
62-63	22.320683621804253	28.021952238841248	27.49929119610527	22.158072943249227
64-65	22.233121064691083	27.944737809710528	27.51995925114523	22.302181874453154
66-67	22.353960762259394	27.978715277732825	27.331189332144373	22.33613462786341
68-69	22.22559348643265	27.902018953983372	27.512750708840944	22.359636850743033
70-71	22.258753447747473	27.71207238354275	27.50807467246399	22.521099496245785
72-73	22.3597582296215	27.77687273266331	27.35331765496084	22.51005138275435
74-75	22.220337304503953	27.917589368382334	27.444294442327212	22.417778884786504
76-77	22.369133743196535	27.85317258625496	27.32174457620357	22.45594909434493
78-79	22.28263822644175	27.82680866480239	27.317492280543977	22.573060828211887
80-81	22.4614682205335	27.805744993538738	27.24783443186197	22.484952354065786
82-83	22.309625140315863	27.871577372555684	27.401301324241317	22.417496162887137
84-85	22.497976031399695	27.86994100940704	27.27801095205866	22.354072007134604
86-87	22.348892244564908	27.89588510710242	27.31816518935311	22.43705745897956
88-89	22.3635660623184	27.878286164783223	27.319729332138188	22.438418440760188
90-91	22.384804614265764	27.9323149329956	27.30671784237838	22.376162610360257
92-93	22.48559654820578	27.860445596630562	27.356340557247165	22.297617297916496
94-95	22.281386450151036	27.958749324448807	27.456029517809412	22.30383470759075
96-97	22.323708659503396	28.03195200256749	27.554170305897053	22.090169032032065
98-99	23.517614984960595	29.908882440204426	27.635542560945353	18.937960013889626
100	22.395028856511335	30.89058473393341	21.57751734999985	25.136869059555405
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2001.0
1	1834.0
2	2496.0
3	3554.5
4	4952.0
5	8186.5
6	10950.0
7	12731.0
8	13877.0
9	13858.5
10	12799.5
11	11549.5
12	12308.0
13	16149.5
14	25138.5
15	37176.5
16	47557.5
17	54281.0
18	56042.0
19	53209.0
20	48059.5
21	42941.0
22	40823.0
23	43507.0
24	51295.5
25	63951.5
26	80749.0
27	99460.5
28	118112.5
29	138806.0
30	159730.0
31	181385.0
32	204527.0
33	225862.5
34	246932.0
35	266191.0
36	286382.0
37	305964.5
38	318924.5
39	326858.0
40	336526.0
41	347477.0
42	354417.0
43	362072.5
44	370407.0
45	375717.5
46	378750.0
47	379208.5
48	379633.5
49	377214.0
50	367783.0
51	356477.0
52	342911.5
53	324712.0
54	306419.5
55	285650.5
56	263106.0
57	239262.5
58	213402.5
59	185735.0
60	153816.5
61	124155.0
62	98369.5
63	74920.5
64	55085.0
65	40901.5
66	31035.5
67	23673.5
68	17959.0
69	12985.0
70	9175.0
71	6614.5
72	4684.0
73	3190.5
74	2115.0
75	1410.5
76	928.5
77	571.5
78	365.0
79	241.0
80	171.0
81	115.0
82	78.0
83	54.5
84	43.5
85	33.0
86	19.5
87	14.0
88	10.5
89	7.5
90	6.5
91	5.0
92	3.0
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.001856224643681332
4	0.013868951172959953
5	0.009186202640036592
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.001730158836493502
26-27	0.0368417764427075
28-29	0.08476076332620958
30-31	0.08222547946854294
32-33	0.03765212086658944
34-35	0.0014640180176060896
36-37	7.387860493515869
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	5.630780277493861E-6
56-57	5.667689212262046E-6
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.011497009630812615
74-75	0.03500582010549816
76-77	0.004693993933519056
78-79	0.0
80-81	0.0
82-83	0.003543037216327703
84-85	0.04091773302397635
86-87	0.044737317757253064
88-89	0.012945306372575262
90-91	6.561885602448686E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	130.0
20-21	495.0
22-23	1167.0
24-25	3144.0
26-27	3980.0
28-29	6374.0
30-31	14999.0
32-33	19090.0
34-35	30889.0
36-37	35925.0
38-39	43466.0
40-41	51101.0
42-43	59755.0
44-45	72705.0
46-47	72267.0
48-49	64717.0
50-51	56046.0
52-53	51997.0
54-55	55862.0
56-57	62173.0
58-59	63438.0
60-61	65127.0
62-63	64744.0
64-65	67555.0
66-67	72556.0
68-69	71076.0
70-71	69364.0
72-73	67507.0
74-75	68581.0
76-77	67974.0
78-79	73313.0
80-81	74799.0
82-83	72745.0
84-85	73193.0
86-87	77344.0
88-89	83892.0
90-91	89984.0
92-93	97023.0
94-95	117380.0
96-97	330469.0
98-99	593288.0
100-101	6413977.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.89400621291183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.20270339256795	77.47871565622675
2	6.9085273394345	11.868021804309164
3	1.6381474071559	4.221211307037429
4	0.5746758847447206	1.9744485605869455
5	0.26997383198681657	1.1594567000999616
6	0.1387711798796048	0.7151767552051128
7	0.07943796315161365	0.4776271430340035
8	0.045088461782737545	0.3098262893197677
9	0.034225727510078435	0.2645806366252894
>10	0.10624186308168787	1.3631811783125254
>50	0.0018141391025128322	0.10190643834897863
>100	3.7338522827227506E-4	0.05403499502896184
>500	1.9424373628081093E-5	0.011812535865173612
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.1093461860015137E-5	0.0	0.0	0.0
3	0.0	2.1093461860015137E-5	0.0	1.0546730930007569E-5	0.0
4	0.0	4.2186923720030274E-5	0.0	1.0546730930007569E-5	0.0
5	0.0	6.328038558004542E-5	0.0	3.164019279002271E-5	0.0
6	4.2186923720030274E-5	6.328038558004542E-5	0.0	3.164019279002271E-5	0.0
7	4.2186923720030274E-5	6.328038558004542E-5	0.0	4.2186923720030274E-5	0.0
8	4.2186923720030274E-5	6.328038558004542E-5	0.0	5.2733654650037845E-5	1.0546730930007569E-5
9	4.2186923720030274E-5	1.7929442581012867E-4	0.0	1.371075020900984E-4	2.1093461860015137E-5
10-11	4.2186923720030274E-5	1.7929442581012867E-4	0.0	1.4765423302010597E-4	2.1093461860015137E-5
12-13	4.2186923720030274E-5	1.8456779127513245E-4	0.0	1.687476948801211E-4	2.1093461860015137E-5
14-15	4.2186923720030274E-5	2.6366827325018924E-4	0.0	2.2148134953015896E-4	3.164019279002271E-5
16-17	6.328038558004542E-5	2.742150041801968E-4	0.0	2.2148134953015896E-4	4.2186923720030274E-5
18-19	6.328038558004542E-5	2.9530846604021194E-4	0.0	2.3202808046016653E-4	4.2186923720030274E-5
20-21	6.328038558004542E-5	3.2694865883023464E-4	0.0	2.5839490778518546E-4	4.2186923720030274E-5
22-23	6.328038558004542E-5	3.691355825502649E-4	0.0	2.7948836964520056E-4	4.2186923720030274E-5
24-25	6.328038558004542E-5	4.3768933359531414E-4	0.0	3.1640192790022704E-4	4.2186923720030274E-5
26-27	6.328038558004542E-5	4.5878279545532924E-4	0.0	3.9022904441028005E-4	4.2186923720030274E-5
28-29	6.328038558004542E-5	4.6405616092033307E-4	0.0	6.433505867304617E-4	4.2186923720030274E-5
30-31	6.328038558004542E-5	4.956963537103557E-4	0.0	0.0020987994550715062	4.2186923720030274E-5
32-33	6.328038558004542E-5	4.956963537103557E-4	0.0	0.005542307103718978	4.2186923720030274E-5
34-35	7.382711651005299E-5	5.537033738253973E-4	0.0	0.009924473805137123	4.2186923720030274E-5
36-37	7.382711651005299E-5	6.11710393940439E-4	0.0	0.019448171834933956	4.2186923720030274E-5
38-39	7.382711651005299E-5	6.169837594054428E-4	0.0	0.043410344507911154	4.2186923720030274E-5
40-41	7.382711651005299E-5	6.749907795204844E-4	0.0	0.0680000476712238	4.2186923720030274E-5
42-43	7.382711651005299E-5	6.960842413804996E-4	0.0	0.08286566491706948	4.7460289185034056E-5
44-45	7.910048197505676E-5	7.910048197505677E-4	0.0	0.10231911011746844	5.800702011504163E-5
46-47	8.437384744006055E-5	8.648319362606207E-4	0.0	0.12227879840250777	6.855375104504921E-5
48-49	8.964721290506434E-5	8.648319362606207E-4	0.0	0.142080285723597	9.492057837006813E-5
50-51	1.1601404023008327E-4	9.755726110257002E-4	0.0	0.16007300869018987	9.492057837006813E-5
52-53	1.2656077116009084E-4	9.80845976490704E-4	0.0	0.20472259408237692	9.492057837006813E-5
54-55	1.2656077116009084E-4	9.80845976490704E-4	0.0	0.2576197230618299	9.492057837006813E-5
56-57	1.2656077116009084E-4	9.80845976490704E-4	0.0	0.3099051416473424	1.0019394383507191E-4
58-59	1.2656077116009084E-4	9.966660728857153E-4	0.0	0.34370741427801665	1.0546730930007569E-4
60-61	1.371075020900984E-4	0.001028306265675738	0.0	0.36779614772215397	1.0546730930007569E-4
62-63	1.371075020900984E-4	0.0010493997275357533	0.0	0.3860208987692071	1.1601404023008327E-4
64-65	1.371075020900984E-4	0.0011812338641608478	0.0	0.39257569204220677	1.1601404023008327E-4
66-67	1.4765423302010597E-4	0.0011917805950908554	0.0	0.3991199385842764	1.2128740569508705E-4
68-69	1.4765423302010597E-4	0.0012392408842758895	0.0	0.4049733742504307	1.2656077116009084E-4
70-71	1.4765423302010597E-4	0.0012656077116009082	0.0	0.40957174893591397	1.2656077116009084E-4
72-73	1.4765423302010597E-4	0.0012761544425309158	0.0	0.4132789248578116	1.2656077116009084E-4
74-75	1.4765423302010597E-4	0.0012867011734609235	0.0	0.4161687291326337	1.2656077116009084E-4
76-77	1.4765423302010597E-4	0.0013394348281109613	0.0	0.41892669927083065	1.3710750209009842E-4
78-79	1.4765423302010597E-4	0.001371075020900984	0.0	0.420751283721722	1.4765423302010597E-4
80-81	1.4765423302010597E-4	0.0013763483863659877	0.0	0.42134717401926736	1.5292759848510975E-4
82-83	1.4765423302010597E-4	0.0013816217518309915	0.0	0.4217690432564677	1.5820096395011355E-4
84-85	1.4765423302010597E-4	0.0013921684827609992	0.0	0.4220432582606479	1.5820096395011355E-4
86-87	1.4765423302010597E-4	0.001423808675551022	0.0	0.422180365762738	1.5820096395011355E-4
88	1.687476948801211E-4	0.001497635792061075	0.0	0.422185639128203	1.5820096395011355E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12175	0.0	48.185486	1
GTATCAA	22735	0.0	38.181202	1
ATCAACG	26800	0.0	31.785385	3
TCAACGC	26775	0.0	31.783049	4
CAACGCA	27410	0.0	30.994682	5
TATCAAC	28065	0.0	30.504608	2
AACGCAG	28215	0.0	30.197998	6
ACGCAGA	32415	0.0	26.245806	7
CGCAGAG	32660	0.0	26.00977	8
GCAGAGT	38575	0.0	22.010445	9
GTACATG	27560	0.0	21.503164	1
TACATGG	27740	0.0	20.88718	2
ACATGGG	27980	0.0	20.03756	3
GAGTACT	22915	0.0	19.856075	12-13
GTGGTAT	5970	0.0	19.496431	1
AGAGTAC	34915	0.0	19.196587	10-11
CAGAGTA	37820	0.0	18.837803	10-11
CATGGGG	21905	0.0	18.334631	4
GTACTTT	25950	0.0	17.468416	14-15
AGTACTT	25285	0.0	17.202654	12-13
>>END_MODULE