FastQCFastQC Report
Fri 27 May 2016
SRR2138921_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138921_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9608838
Sequences flagged as poor quality0
Sequence length19-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135350.047.14931
GTATCAA243250.035.2655141
ATCAACG281350.029.6099363
TCAACGC283050.029.4474334
CAACGCA286900.029.0070295
AACGCAG295200.028.3138146
TATCAAC305300.027.657252
ACGCAGA335000.024.8982497
CGCAGAG336600.024.7798988
GTACATG252850.021.6848581
TACATGG250350.021.3291572
GCAGAGT390300.021.3148969
ACATGGG243800.021.0115343
GTGGTAT68900.019.9735951
TGGTATC64750.019.6446362
GAGTACT279200.019.43759212-13
AGAGTAC359600.019.35530910-11
CATGGGG166050.018.8497584
CAGAGTA389050.018.7997410-11
AGTACTT299050.017.75540212-13
Produced by FastQC (version 0.11.5)