##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138921_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9608838
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.681764225809616	33.0	33.0	33.0	33.0	33.0
2	32.6865070469499	33.0	33.0	33.0	33.0	33.0
3	32.69819763846576	33.0	33.0	33.0	33.0	33.0
4	36.621406043061604	37.0	37.0	37.0	37.0	37.0
5	36.63297799380112	37.0	37.0	37.0	37.0	37.0
6	36.56794713367007	37.0	37.0	37.0	37.0	37.0
7	36.542476207841155	37.0	37.0	37.0	37.0	37.0
8	36.538120842499374	37.0	37.0	37.0	37.0	37.0
9	36.583921177565905	37.0	37.0	37.0	37.0	37.0
10-11	36.56166432403169	37.0	37.0	37.0	37.0	37.0
12-13	36.553660806853024	37.0	37.0	37.0	37.0	37.0
14-15	38.64163476374563	40.0	37.0	40.0	37.0	40.0
16-17	38.578374877378515	40.0	37.0	40.0	37.0	40.0
18-19	38.59753161620583	40.0	37.0	40.0	37.0	40.0
20-21	38.61404992853541	40.0	37.0	40.0	37.0	40.0
22-23	38.610138492162264	40.0	37.0	40.0	37.0	40.0
24-25	38.61251462745767	40.0	37.0	40.0	37.0	40.0
26-27	38.531019923628264	40.0	37.0	40.0	37.0	40.0
28-29	38.41611019727391	40.0	37.0	40.0	37.0	40.0
30-31	38.287186663499135	40.0	37.0	40.0	37.0	40.0
32-33	38.17905232429092	40.0	37.0	40.0	37.0	40.0
34-35	38.157286275432305	40.0	37.0	40.0	37.0	40.0
36-37	38.14274278708298	40.0	37.0	40.0	37.0	40.0
38-39	38.103400716276596	40.0	37.0	40.0	37.0	40.0
40-41	38.07966737128146	40.0	37.0	40.0	37.0	40.0
42-43	38.02920962759359	40.0	37.0	40.0	33.0	40.0
44-45	37.943104310500544	40.0	37.0	40.0	33.0	40.0
46-47	37.84577377246844	40.0	37.0	40.0	33.0	40.0
48-49	37.793308887914996	40.0	37.0	40.0	33.0	40.0
50-51	37.779643029109444	40.0	37.0	40.0	33.0	40.0
52-53	37.72929093266745	40.0	37.0	40.0	33.0	40.0
54-55	37.62256879631609	40.0	37.0	40.0	33.0	40.0
56-57	37.45824371481636	40.0	37.0	40.0	33.0	40.0
58-59	37.282686637200186	40.0	37.0	40.0	33.0	40.0
60-61	37.12579101712736	37.0	37.0	40.0	33.0	40.0
62-63	36.997544727793326	37.0	37.0	40.0	33.0	40.0
64-65	36.842729715290034	37.0	37.0	40.0	33.0	40.0
66-67	36.644946877027515	37.0	37.0	40.0	33.0	40.0
68-69	36.44950546000995	37.0	37.0	40.0	33.0	40.0
70-71	36.23172336668247	37.0	37.0	40.0	33.0	40.0
72-73	35.94109976113327	37.0	37.0	37.0	33.0	40.0
74-75	35.57512296333152	37.0	33.0	37.0	33.0	40.0
76-77	33.54354573929888	35.0	33.0	37.0	27.0	37.0
78-79	35.12477691291192	37.0	33.0	37.0	33.0	37.0
80-81	35.30179352127704	37.0	33.0	37.0	33.0	37.0
82-83	35.07704605044967	37.0	33.0	37.0	33.0	37.0
84-85	34.90252194940285	37.0	33.0	37.0	33.0	37.0
86-87	34.77958690210038	37.0	33.0	37.0	33.0	37.0
88-89	34.65539543862756	37.0	33.0	37.0	33.0	37.0
90-91	34.57246075812457	37.0	33.0	37.0	33.0	37.0
92-93	34.51802484130776	37.0	33.0	37.0	33.0	37.0
94-95	34.46608108871338	37.0	33.0	37.0	33.0	37.0
96-97	34.47840223497655	37.0	33.0	37.0	33.0	37.0
98-99	34.509763616910625	37.0	33.0	37.0	33.0	37.0
100	34.4524740149941	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	28.0
20	70.0
21	272.0
22	966.0
23	2918.0
24	7137.0
25	14744.0
26	26705.0
27	43141.0
28	66085.0
29	93896.0
30	130553.0
31	175378.0
32	235552.0
33	320799.0
34	448677.0
35	668108.0
36	1179523.0
37	2782018.0
38	3333004.0
39	79258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.776439357183456	21.355266890752034	14.608238790163805	23.2600549619007
2	13.901571543719992	21.26783994305164	43.45771880810162	21.372869705126757
3	17.808546502957334	26.69361343990349	31.0910633242233	24.406776732915876
4	11.68362917555692	18.230279249166237	40.63090667154551	29.455184903731336
5	12.398398224634446	38.274170092158904	36.740654801340185	12.586776881866465
6	28.57724315885022	39.326909247507345	19.29208297611012	12.803764617532318
7	25.178892598668018	33.24639253986798	24.410922527781196	17.163792333682803
8	22.923063121680272	38.53848925333116	21.819828786789827	16.71861883819875
9	24.669996517789144	17.167216264859498	22.522431952750168	35.64035526460119
10-11	23.30718517014501	27.623682293156726	29.8664364340602	19.20269610263806
12-13	23.93886140649052	25.15952569531971	30.209207372475717	20.69240552571405
14-15	20.95230973818062	26.718761415272063	27.974542811524138	24.354386035023172
16-17	20.365761187773174	29.75304089838959	28.828667940910236	21.052529972927008
18-19	20.17443732530406	28.84314940058309	30.845014766613822	20.137398507499032
20-21	20.907907450932314	28.1065810960079	30.89162046104686	20.093890992012927
22-23	20.615242967380137	28.005877319337458	30.74812327903169	20.630756434250717
24-25	20.424524832655557	28.093161047450447	30.86557629725681	20.61673782263719
26-27	20.469672228197826	28.31301193354404	30.688666824743404	20.528649013514727
28-29	20.337813124470554	28.43108955149004	30.709702001394994	20.521395322644413
30-31	20.64015992472707	28.282185453669122	30.62368484498661	20.453969776617196
32-33	20.4164941842644	28.43872604680576	30.540358582945547	20.604421185984297
34-35	20.55437632990287	28.52040415427345	30.53863488973658	20.386584626087103
36-37	20.548934787250584	28.466362744561412	30.453886700602762	20.530815767585242
38-39	20.426102313885046	28.6430690696357	30.47045251761337	20.460376098865883
40-41	20.71516930036498	28.49598151797126	30.292165930279097	20.496683251384667
42-43	20.555518334216494	28.624178561555887	30.09600113523303	20.724301968994588
44-45	20.688181959428796	28.803939825502926	29.86484492976625	20.643033285302028
46-47	20.727218889087776	28.81936806632301	29.550939729309704	20.90247331527951
48-49	20.72658740230187	28.901208848075022	29.52917591850993	20.843027831113172
50-51	20.712639676737094	29.03931107516322	29.35075447168301	20.897294776416672
52-53	20.824118408637798	28.982678943386485	29.252925083670505	20.940277564305212
54-55	20.860989425293894	28.933583834060784	29.241068552301897	20.96435818834343
56-57	20.864411481364506	28.997603273035498	29.172802416546073	20.965182829053923
58-59	20.82347855664445	29.060831331856114	29.220116243437676	20.89557386806176
60-61	20.9230557858292	29.02409457638996	29.20182302156209	20.851026616218753
62-63	20.854774676416866	29.054393874072964	29.180678394512228	20.91015305499794
64-65	20.81763873776528	29.079097672935696	29.085892872984843	21.017370716314183
66-67	20.923847535279428	29.11752407214212	28.924546880885167	21.034081511693287
68-69	20.858836998604854	29.068479261051255	29.046187838708114	21.026495901635776
70-71	20.945157154700105	28.811711573226166	29.073378511866128	21.1697527602076
72-73	20.99135382544228	28.890172714095108	29.007775651662854	21.110697808799763
74-75	20.879203221895047	29.056544874152866	28.940480358062054	21.123771545890026
76-77	20.993417959399196	29.01853615541113	28.82586851356547	21.1621773716242
78-79	21.050305964360565	28.977186833607462	28.799527201953897	21.172980000078077
80-81	20.947092184566262	29.01496693688866	28.822512503608483	21.215428374936597
82-83	21.086492747373782	28.963384072733817	28.902641666632118	21.047481513260283
84-85	21.2006523328692	28.959939070699114	28.789610851206643	21.049797745225042
86-87	21.014881111608368	29.01689366938866	28.837453412641235	21.13077180636174
88-89	21.11360751234886	28.98555333417509	28.823889948456145	21.07694920501991
90-91	21.164323981171123	28.99854426128362	28.810650270240757	21.0264814873045
92-93	21.218736398974052	28.97007247928932	28.782879485302782	21.028311636433845
94-95	21.001564237294332	29.06769746292325	28.847877275033305	21.082861024749118
96-97	21.072314858008927	29.180404776342368	28.952217441422373	20.795062924226336
98-99	22.182996624925316	31.041689655980605	29.15984170481557	17.61547201427851
100	21.229921864725256	31.99709737627835	23.094294815747435	23.678685943248958
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3342.0
1	2807.5
2	3337.0
3	4825.5
4	6524.0
5	10507.0
6	14146.5
7	16891.5
8	18818.5
9	18692.0
10	16915.5
11	14735.0
12	15104.0
13	18902.5
14	28660.0
15	42821.0
16	54558.0
17	61876.5
18	64852.5
19	63451.0
20	59608.0
21	55646.5
22	54180.0
23	57555.0
24	66634.5
25	81239.5
26	100397.5
27	122505.0
28	145572.0
29	170948.5
30	196487.5
31	220975.5
32	246567.5
33	271858.5
34	298578.5
35	323114.5
36	348487.0
37	372178.0
38	384470.5
39	391366.0
40	401559.5
41	409423.5
42	410227.0
43	413134.5
44	415898.5
45	415329.5
46	410479.0
47	401252.0
48	389744.0
49	370638.5
50	347075.5
51	320425.5
52	287367.0
53	254750.0
54	222963.0
55	191206.0
56	163207.5
57	137276.0
58	115405.0
59	97496.5
60	78140.0
61	60414.0
62	45974.5
63	33820.0
64	24751.0
65	17972.0
66	13335.0
67	9987.5
68	7545.0
69	5464.0
70	3859.0
71	2882.0
72	2141.5
73	1467.0
74	1004.5
75	701.5
76	486.0
77	342.0
78	258.5
79	185.0
80	120.0
81	79.5
82	52.5
83	35.5
84	29.5
85	23.5
86	16.5
87	13.0
88	9.0
89	5.5
90	4.0
91	3.5
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0013841423905783406
3	1.977346272254772E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.203542821723084E-5
12-13	0.00695193320982204
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.5612342618479207E-5
24-25	3.383286958011743E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	5.762224428164705E-4
36-37	0.02504802755587918
38-39	0.12164251088312251
40-41	0.143400545469018
42-43	0.03166728721161284
44-45	0.002253349396828381
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.007169642257576045
64-65	0.07437153566075697
66-67	0.18215919348668969
68-69	0.1890753684764463
70-71	0.06972374927405152
72-73	0.002235076394911178
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	132.0
20-21	628.0
22-23	1424.0
24-25	2800.0
26-27	4372.0
28-29	7752.0
30-31	17982.0
32-33	22102.0
34-35	30104.0
36-37	43096.0
38-39	49608.0
40-41	53224.0
42-43	60893.0
44-45	70444.0
46-47	69795.0
48-49	60766.0
50-51	51906.0
52-53	47254.0
54-55	50621.0
56-57	51133.0
58-59	50471.0
60-61	48251.0
62-63	52352.0
64-65	55439.0
66-67	63504.0
68-69	62561.0
70-71	64121.0
72-73	60440.0
74-75	60168.0
76-77	55526.0
78-79	60445.0
80-81	63967.0
82-83	62250.0
84-85	69167.0
86-87	76933.0
88-89	82711.0
90-91	88431.0
92-93	94533.0
94-95	115046.0
96-97	313282.0
98-99	614437.0
100-101	6698767.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.65891589078596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.89858926262045	70.09146002361659
2	8.596359303169532	13.867460440026532
3	2.3734091326013087	5.743098228027367
4	0.9433760800253871	3.0436676756858825
5	0.4719788501665007	1.9034651188904825
6	0.23531518168984192	1.1388160468647586
7	0.14774222267735881	0.8341709258715598
8	0.09328106548616151	0.6019159692200958
9	0.055307593639525816	0.4014945489143054
>10	0.18202897458364348	2.1295069813766982
>50	0.00199436042082833	0.11181718016524567
>100	5.243322166255705E-4	0.0774427291281276
>500	9.364070272561781E-5	0.0556841322123207
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0407085643446169E-5	0.0	0.0	0.0
3	0.0	1.0407085643446169E-5	0.0	0.0	0.0
4	0.0	6.244251386067701E-5	0.0	1.0407085643446169E-5	0.0
5	0.0	6.244251386067701E-5	0.0	1.0407085643446169E-5	0.0
6	0.0	6.244251386067701E-5	0.0	1.0407085643446169E-5	0.0
7	0.0	7.284959950412318E-5	0.0	1.0407085643446169E-5	0.0
8	0.0	1.1447794207790785E-4	0.0	1.0407085643446169E-5	1.0407085643446169E-5
9	0.0	2.601771410861542E-4	0.0	1.0407085643446169E-5	1.0407085643446169E-5
10-11	0.0	2.705842267296004E-4	0.0	3.642479975206159E-5	3.122125693033851E-5
12-13	0.0	2.705842267296004E-4	0.0	4.1628342573784676E-5	3.122125693033851E-5
14-15	0.0	3.8506216880750826E-4	0.0	5.7238971038953926E-5	3.122125693033851E-5
16-17	0.0	4.058763400944006E-4	0.0	8.325668514756935E-5	7.284959950412318E-5
18-19	0.0	4.839294824202469E-4	0.0	9.366377079101551E-5	9.366377079101551E-5
20-21	0.0	5.775932532112623E-4	0.0	1.2488502772135403E-4	1.1447794207790785E-4
22-23	0.0	6.400357670719393E-4	0.0	1.5610628465169253E-4	1.1447794207790785E-4
24-25	0.0	7.336995378629549E-4	0.0	1.9253108440375413E-4	1.2488502772135403E-4
26-27	0.0	7.701243376150165E-4	0.0	2.289558841558157E-4	1.4569919900824635E-4
28-29	0.0	7.701243376150165E-4	0.0	5.880003388547086E-4	1.7171691311686178E-4
30-31	0.0	9.522483363753245E-4	0.0	0.0019253108440375412	1.7692045593858488E-4
32-33	0.0	9.7826605048394E-4	0.0	0.004906940880884869	1.8212399876030796E-4
34-35	0.0	0.001108354621027017	0.0	0.008820005082820628	1.8732754158203103E-4
36-37	0.0	0.001238443191570094	0.0	0.015569000122595468	1.8732754158203103E-4
38-39	0.0	0.0012592573628569864	0.0	0.03210585921003144	1.8732754158203103E-4
40-41	0.0	0.0013945494762217868	0.0	0.05012572800165847	1.9253108440375413E-4
42-43	0.0	0.0014205671903304022	0.0	0.06147985843865824	2.0814171286892338E-4
44-45	0.0	0.0016339124460210486	0.0	0.07365614864149025	2.1854879851236956E-4
46-47	0.0	0.0018264435304248027	0.0	0.08516118182032001	2.1854879851236956E-4
48-49	1.0407085643446169E-5	0.001842054158889972	0.0	0.09722299408107411	2.2375234133409263E-4
50-51	1.0407085643446169E-5	0.0020606029574023414	0.0	0.10926919571336305	2.3936296979926188E-4
52-53	1.0407085643446169E-5	0.00209182421433268	0.0	0.13736312340784598	2.3936296979926188E-4
54-55	1.0407085643446169E-5	0.0021126383856195723	0.0	0.1694689826178774	2.44566512620985E-4
56-57	1.0407085643446169E-5	0.0021230454712630187	0.0	0.19995654001035296	2.5497359826443113E-4
58-59	1.0407085643446169E-5	0.0021282490140847416	0.0	0.21898069256657257	2.601771410861542E-4
60-61	1.0407085643446169E-5	0.0021594702710150803	0.0	0.2343155332621905	2.8099131237304656E-4
62-63	1.0407085643446169E-5	0.002206302156410588	0.0	0.24657508015017007	2.8099131237304656E-4
64-65	1.0407085643446169E-5	0.0023624084410622803	0.0	0.2503632593243845	2.8099131237304656E-4
66-67	1.0407085643446169E-5	0.002388426155170896	0.0	0.25430234124042883	2.8099131237304656E-4
68-69	1.0407085643446169E-5	0.0024352580405664036	0.0	0.25839752944112493	2.966019408382158E-4
70-71	1.0407085643446169E-5	0.0024612757546750193	0.0	0.26219091215816104	3.018054836599389E-4
72-73	1.0407085643446169E-5	0.0024664792974967422	0.0	0.2651725421950084	3.1221256930338506E-4
74-75	1.0407085643446169E-5	0.0025549395254660344	0.0	0.2675245435504272	3.2261965494683126E-4
76-77	1.0407085643446169E-5	0.0026329926677918806	0.0	0.26963197839322506	3.2782319776855434E-4
78-79	1.0407085643446169E-5	0.002653806839078773	0.0	0.2710785632976641	3.382302834120005E-4
80-81	1.0407085643446169E-5	0.0026642139247222193	0.0	0.2715416786087974	3.4863736905544663E-4
82-83	1.0407085643446169E-5	0.0026694174675439422	0.0	0.27200999746275256	3.642479975206159E-4
84-85	1.0407085643446169E-5	0.002695435181652558	0.0	0.2722233427184432	3.642479975206159E-4
86-87	1.0407085643446169E-5	0.0027474706098697885	0.0	0.27229619231794727	3.8506216880750826E-4
88	1.0407085643446169E-5	0.0028723556375911427	0.0	0.27231180294641244	3.8506216880750826E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13535	0.0	47.1493	1
GTATCAA	24325	0.0	35.265514	1
ATCAACG	28135	0.0	29.609936	3
TCAACGC	28305	0.0	29.447433	4
CAACGCA	28690	0.0	29.007029	5
AACGCAG	29520	0.0	28.313814	6
TATCAAC	30530	0.0	27.65725	2
ACGCAGA	33500	0.0	24.898249	7
CGCAGAG	33660	0.0	24.779898	8
GTACATG	25285	0.0	21.684858	1
TACATGG	25035	0.0	21.329157	2
GCAGAGT	39030	0.0	21.314896	9
ACATGGG	24380	0.0	21.011534	3
GTGGTAT	6890	0.0	19.973595	1
TGGTATC	6475	0.0	19.644636	2
GAGTACT	27920	0.0	19.437592	12-13
AGAGTAC	35960	0.0	19.355309	10-11
CATGGGG	16605	0.0	18.849758	4
CAGAGTA	38905	0.0	18.79974	10-11
AGTACTT	29905	0.0	17.755402	12-13
>>END_MODULE