FastQCFastQC Report
Fri 27 May 2016
SRR2138920_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138920_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7548525
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140700.052.4678761
GTATCAA261300.038.4911
ATCAACG322600.030.6443043
CAACGCA326050.030.3463955
TCAACGC327700.030.2067014
TATCAAC332000.030.0355682
AACGCAG336600.029.5410756
ACGCAGA377700.026.235547
CGCAGAG381050.025.9823448
GCAGAGT438150.022.4884749
TACATGG292850.022.4073182
TGGTATC57250.021.7537562
GTACATG309250.021.7328341
ACATGGG292850.021.5566333
GTGGTAT61200.021.4724561
GAGTACT281150.020.7601512-13
CAGAGTA423650.020.27232710-11
AGAGTAC411500.019.4210-11
GTACTTT306750.019.04784414-15
CATGGGG183500.018.5820374
Produced by FastQC (version 0.11.5)