##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138920_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7548525
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69379673512375	33.0	33.0	33.0	33.0	33.0
2	32.69318191302274	33.0	33.0	33.0	33.0	33.0
3	32.70030012485883	33.0	33.0	33.0	33.0	33.0
4	36.629260948330966	37.0	37.0	37.0	37.0	37.0
5	36.643484786762976	37.0	37.0	37.0	37.0	37.0
6	36.58718835799047	37.0	37.0	37.0	37.0	37.0
7	36.562916331336254	37.0	37.0	37.0	37.0	37.0
8	36.56276279140627	37.0	37.0	37.0	37.0	37.0
9	36.601312574310874	37.0	37.0	37.0	37.0	37.0
10-11	36.58110061237129	37.0	37.0	37.0	37.0	37.0
12-13	36.57136785001043	37.0	37.0	37.0	37.0	37.0
14-15	38.65886527500406	40.0	37.0	40.0	37.0	40.0
16-17	38.597802418353254	40.0	37.0	40.0	37.0	40.0
18-19	38.63077024981702	40.0	37.0	40.0	37.0	40.0
20-21	38.648559711299896	40.0	37.0	40.0	37.0	40.0
22-23	38.64284230270242	40.0	37.0	40.0	37.0	40.0
24-25	38.641851217102555	40.0	37.0	40.0	37.0	40.0
26-27	38.55481238929957	40.0	37.0	40.0	37.0	40.0
28-29	38.44272908991358	40.0	37.0	40.0	37.0	40.0
30-31	38.2949111322552	40.0	37.0	40.0	37.0	40.0
32-33	38.18022035466038	40.0	37.0	40.0	37.0	40.0
34-35	38.157840823969366	40.0	37.0	40.0	37.0	40.0
36-37	38.140883195851444	40.0	37.0	40.0	37.0	40.0
38-39	38.093757985581874	40.0	37.0	40.0	37.0	40.0
40-41	38.06390108343035	40.0	37.0	40.0	37.0	40.0
42-43	38.0060767412636	40.0	37.0	40.0	33.0	40.0
44-45	37.907863530547324	40.0	37.0	40.0	33.0	40.0
46-47	37.79456088066596	40.0	37.0	40.0	33.0	40.0
48-49	37.726190607008085	40.0	37.0	40.0	33.0	40.0
50-51	37.70464634115601	40.0	37.0	40.0	33.0	40.0
52-53	37.64561476285032	40.0	37.0	40.0	33.0	40.0
54-55	37.530013654706465	40.0	37.0	40.0	33.0	40.0
56-57	37.34903056510482	40.0	37.0	40.0	33.0	40.0
58-59	37.159251705137585	37.0	37.0	40.0	33.0	40.0
60-61	36.98382411105186	37.0	37.0	40.0	33.0	40.0
62-63	36.84239968913654	37.0	37.0	40.0	33.0	40.0
64-65	36.67307504295671	37.0	37.0	40.0	33.0	40.0
66-67	36.460228284828254	37.0	37.0	40.0	33.0	40.0
68-69	36.26018480576072	37.0	37.0	40.0	33.0	40.0
70-71	36.03811709285249	37.0	37.0	38.5	33.0	40.0
72-73	35.75099376604658	37.0	35.0	37.0	33.0	40.0
74-75	35.38645422025787	37.0	33.0	37.0	33.0	40.0
76-77	33.29584715915559	35.0	33.0	37.0	27.0	37.0
78-79	34.939783482781806	37.0	33.0	37.0	33.0	37.0
80-81	35.16003056794429	37.0	33.0	37.0	33.0	37.0
82-83	34.96010136754626	37.0	33.0	37.0	33.0	37.0
84-85	34.80828574334224	37.0	33.0	37.0	33.0	37.0
86-87	34.71492981891143	37.0	33.0	37.0	33.0	37.0
88-89	34.61380390912761	37.0	33.0	37.0	33.0	37.0
90-91	34.54198165908713	37.0	33.0	37.0	33.0	37.0
92-93	34.49950621517266	37.0	33.0	37.0	33.0	37.0
94-95	34.45820002336113	37.0	33.0	37.0	33.0	37.0
96-97	34.47297424527257	37.0	33.0	37.0	33.0	37.0
98-99	34.50840536037663	37.0	33.0	37.0	33.0	37.0
100	34.46119870932553	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	1.0
19	17.0
20	45.0
21	232.0
22	843.0
23	2309.0
24	5745.0
25	11508.0
26	20843.0
27	34261.0
28	51845.0
29	73823.0
30	102366.0
31	137688.0
32	185087.0
33	251591.0
34	352968.0
35	530145.0
36	972482.0
37	2320818.0
38	2430897.0
39	63007.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.807391749698354	20.792329732276137	14.50080836995418	22.899470148071334
2	14.550379610198238	21.021286494980856	42.68603377059591	21.742300124224982
3	17.848591981709784	27.10658265722923	30.819709354830888	24.2251160062301
4	11.998001198644927	18.090490273330257	39.85267848389963	30.058830044125184
5	12.749419621637289	38.42347192643224	35.6999593562927	13.127149095637769
6	28.80156056978019	38.60420413259544	19.23263154059899	13.361603757025378
7	25.877797318019084	33.358649537492425	23.69979565544262	17.063757489045873
8	24.2996082016023	36.65554528864911	21.380163674360222	17.66468283538837
9	25.95700484531746	16.545232346716745	21.76531176620598	35.732451041759816
10-11	24.04620904289548	26.96379986944465	29.05677899218026	19.933212095479615
12-13	25.041454909670957	24.724945548201102	29.29650528089709	20.937094261230847
14-15	22.127091054212578	25.759621912890267	27.54595765397876	24.567329378918398
16-17	20.975018298276815	28.691512580272306	28.370039179839768	21.96342994161111
18-19	20.782325023762922	27.70119327948175	30.734507734954843	20.781973961800485
20-21	21.820896805660002	26.889027388885932	30.625069593157328	20.66500621229674
22-23	21.512823250962942	26.92301067769555	30.4605272920372	21.10363877930431
24-25	21.186863262499802	27.038285514151887	30.76203832328061	21.0128129000677
26-27	21.082026947413684	27.2924730816489	30.464357835800293	21.16114213513712
28-29	20.953148417908057	27.48798917555303	30.4748646529671	21.083997753571815
30-31	21.30683930745386	27.284879341957936	30.323904644240436	21.084376706347776
32-33	21.041366083612058	27.495381633686698	30.21090251168331	21.252349771017926
34-35	21.291351519866346	27.54434571051106	30.22958224665186	20.934720522970736
36-37	21.297475747736687	27.414664837547853	30.190102870858095	21.09775654385737
38-39	21.127095297319755	27.570121300742752	30.21981362387863	21.08296977805887
40-41	21.34473946574985	27.460591472183744	30.08751071559092	21.10715834647549
42-43	21.33260794599939	27.614921409831204	29.624189426272746	21.428281217896657
44-45	21.320362424731996	27.93702757135246	29.315519763229375	21.427090240686177
46-47	21.511775263480953	27.905493443879426	28.932923333766713	21.649807958872913
48-49	21.624280180586485	28.0539863600342	28.694102724775234	21.627630734604082
50-51	21.570572857730514	28.0767830869778	28.536035556245494	21.816608499046193
52-53	21.64214170576723	28.051880929089407	28.312821685014182	21.99315568012918
54-55	21.733298424768808	27.982237316074787	28.339139691656662	21.945324567499743
56-57	21.77167580948361	28.028288775888004	28.2523453248219	21.94769008980649
58-59	21.705993965342433	28.0423700136099	28.472031816093317	21.779604204954346
60-61	21.76846923200197	28.24184846088797	28.288120432373304	21.701561874736758
62-63	21.79817359305602	28.060659652327953	28.273358878071814	21.867807876544212
64-65	21.760155101978917	28.095132472511263	28.15056599987176	21.994146425638057
66-67	21.732583895358548	28.228673472338055	27.982705140286978	22.05603749201642
68-69	21.752538998513142	28.148944679053546	28.044045608969626	22.054470713463683
70-71	21.920477165728695	27.729619188822795	28.081408730376197	22.268494915072313
72-73	21.917825134119614	27.924411294463138	28.05531435637829	22.10244921503896
74-75	21.899017893150084	28.04692153223378	27.96123394434784	22.092826630268288
76-77	21.962500849777104	27.934943858835492	27.93074541671362	22.171809874673787
78-79	21.881790400018783	27.97066085466206	27.89131447040456	22.2562342749146
80-81	21.841610862933624	27.92054367745587	27.977128038783196	22.260717420827312
82-83	22.265735553175283	27.735795988445794	28.061503839862006	21.936964618516914
84-85	22.28778567800505	27.867315596135484	27.756176866358214	22.088721859501252
86-87	21.99933524030265	27.9796965675774	27.928174025976777	22.09279416614317
88-89	22.287965286298725	27.702217098681587	27.84906486907689	22.1607527459428
90-91	22.300656038623025	27.693913413403326	27.90464710259154	22.100783445382106
92-93	22.477377889457976	27.577236125419358	27.849050900194012	22.096335084928654
94-95	22.10099901495379	27.619049095398946	28.03109259864973	22.248859290997533
96-97	22.19963588981688	27.811431822977028	28.08384688399087	21.905085403215224
98-99	23.220196926773067	29.663432131668184	28.27796409277916	18.83840684877959
100	22.34571021634889	30.35456813075406	22.08275585593032	25.216965796966733
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1887.0
1	1885.5
2	3336.0
3	5084.5
4	6907.0
5	11608.5
6	15521.5
7	17879.0
8	19162.5
9	18338.0
10	16167.0
11	13946.5
12	15013.5
13	20312.5
14	32365.5
15	48957.5
16	62613.5
17	70008.5
18	70370.0
19	65290.0
20	57113.0
21	47891.0
22	41174.5
23	39398.0
24	42081.0
25	48553.0
26	59439.5
27	72383.0
28	83832.5
29	99417.0
30	116462.5
31	132769.0
32	154225.0
33	175338.0
34	195667.0
35	215964.0
36	237495.5
37	261554.0
38	275558.5
39	281240.5
40	288693.0
41	292218.0
42	293431.0
43	302373.0
44	315871.0
45	328653.5
46	334798.5
47	334424.0
48	331334.5
49	323440.0
50	308149.5
51	289547.5
52	270739.0
53	248260.0
54	226156.5
55	200628.0
56	172746.5
57	146696.5
58	125269.0
59	106497.0
60	82820.5
61	62095.0
62	47617.5
63	35164.5
64	24930.5
65	17581.5
66	12777.0
67	10044.5
68	7947.5
69	6218.5
70	4656.5
71	2764.0
72	1821.5
73	1294.0
74	858.0
75	581.5
76	389.0
77	232.0
78	128.0
79	92.0
80	71.5
81	61.0
82	51.0
83	36.5
84	29.5
85	25.5
86	19.5
87	16.5
88	14.0
89	13.0
90	11.0
91	7.0
92	3.5
93	1.0
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.3247621224013964E-5
2	0.0016427050317777312
3	1.0598096979211171E-4
4	1.3247621224013964E-5
5	1.3247621224013964E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	3.311905306003491E-5
12-13	0.0074849059915678895
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.624537698356048E-6
24-25	3.5116056246911197E-4
26-27	0.0
28-29	1.3262651359179722E-5
30-31	1.3278969233292233E-5
32-33	1.3307493729176266E-5
34-35	5.804051227756974E-4
36-37	0.025017231563833373
38-39	0.12198726883543681
40-41	0.1448759610285693
42-43	0.03147284548608572
44-45	0.002098305474328124
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.007307488379742942
64-65	0.07558419409815073
66-67	0.18537508055028362
68-69	0.19287228186886018
70-71	0.07248827777610767
72-73	0.00249238583748623
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	115.0
20-21	486.0
22-23	1083.0
24-25	2070.0
26-27	3347.0
28-29	7094.0
30-31	15420.0
32-33	18652.0
34-35	24037.0
36-37	31997.0
38-39	37079.0
40-41	45315.0
42-43	54609.0
44-45	64196.0
46-47	65786.0
48-49	53688.0
50-51	43888.0
52-53	39782.0
54-55	40200.0
56-57	41809.0
58-59	47160.0
60-61	48639.0
62-63	51906.0
64-65	53964.0
66-67	61977.0
68-69	59260.0
70-71	60204.0
72-73	61187.0
74-75	57658.0
76-77	52713.0
78-79	57347.0
80-81	62660.0
82-83	60231.0
84-85	65838.0
86-87	74119.0
88-89	81587.0
90-91	84926.0
92-93	88179.0
94-95	97626.0
96-97	249209.0
98-99	472628.0
100-101	5008854.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.48496251980278
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.63092110571768	65.49477791307005
2	8.741610972885459	13.37203575247682
3	2.686431020401537	6.164147277223413
4	1.1871736291336634	3.6320372211514593
5	0.6124371825252386	2.3421117475588242
6	0.3531458097652283	1.6206206414351332
7	0.207184276209427	1.1092537120399344
8	0.13531446752077267	0.8279617581370694
9	0.1029083894787469	0.70838498810297
>10	0.3349587033680358	3.9032667785380184
>50	0.005667333540936819	0.2841449369755864
>100	0.001817336864246043	0.2678590353603343
>500	3.125618846026013E-4	0.16656703323145422
>1k	1.172107044709024E-4	0.10683120469898841
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	9.273334856809775E-5	0.0	0.0	0.0	0.0
3	9.273334856809775E-5	0.0	0.0	0.0	0.0
4	9.273334856809775E-5	0.0	0.0	0.0	0.0
5	2.2520956080823736E-4	0.0	0.0	0.0	0.0
6	3.179429093763351E-4	0.0	0.0	0.0	0.0
7	6.623810612006981E-4	0.0	0.0	0.0	0.0
8	7.0212392487274E-4	0.0	0.0	0.0	0.0
9	7.0212392487274E-4	0.0	0.0	1.3247621224013962E-5	0.0
10-11	7.15371546096754E-4	0.0	0.0	1.3247621224013962E-5	0.0
12-13	9.405811069049914E-4	0.0	0.0	1.3247621224013962E-5	0.0
14-15	0.0013048906905653752	0.0	0.0	2.6495242448027925E-5	0.0
16-17	0.00140424784974548	0.0	0.0	3.974286367204189E-5	0.0
18-19	0.0017023193272857943	0.0	0.0	6.623810612006982E-5	0.0
20-21	0.0018546669713619549	0.0	0.0	1.457238334641536E-4	0.0
22-23	0.0019407765093180456	0.0	0.0	4.8353817467650963E-4	0.0
24-25	0.0024243146839945553	0.0	0.0	0.0013048906905653752	0.0
26-27	0.002437562305218569	0.0	0.0	0.002861486184387016	0.0
28-29	0.003212548146823386	0.0	0.0	0.007816096522168239	0.0
30-31	0.003537114866811728	0.0	0.0	0.009995330213518536	0.0
32-33	0.003570233919871763	0.0	0.0	0.01465849288437145	0.0
34-35	0.0038484339655760562	0.0	0.0	0.01978532229806485	0.0
36-37	0.004252486412908482	0.0	0.0	0.027369585448812846	0.0
38-39	0.004636667428404887	0.0	0.0	0.048837355642327475	0.0
40-41	0.004709529345136964	0.0	0.0	0.0717292451174236	0.0
42-43	0.005246058004709529	0.0	0.0	0.08597706174385061	0.0
44-45	0.0058289533385661434	0.0	0.0	0.10224514060693976	0.0
46-47	0.006716543960575079	0.0	0.0	0.11822839561371262	0.0
48-49	0.007094101165459477	0.0	0.0	0.135682136576351	0.0
50-51	0.007100724976071484	0.0	0.0	0.15196346306066416	0.0
52-53	0.007100724976071484	0.0	0.0	0.20534475278282843	0.0
54-55	0.007173586892803561	0.0	0.0	0.2695493490450121	0.0
56-57	0.007590886961360001	0.0	0.0	0.324480610450386	0.0
58-59	0.007630629825032043	0.0	0.0	0.3450607900218917	0.0
60-61	0.007690244120540105	0.0	0.0	0.3601564544066556	0.0
62-63	0.007743234605436161	0.0	0.0	0.3714434276895155	0.0
64-65	0.00785583938584028	0.0	0.0	0.37594099509506823	0.0
66-67	0.0082532680225607	0.0	0.0	0.3805577910916371	0.0
68-69	0.008478477583368936	0.0	0.0	0.3850089918229058	0.0
70-71	0.008743430007849214	0.0	0.0	0.3891025067811261	0.0
72-73	0.00897526337926946	0.0	0.0	0.3925800073524298	0.0
74-75	0.009101115780897593	0.0	0.0	0.39576606025680516	0.0
76-77	0.00917397769762967	0.0	0.0	0.39826986066814374	0.0
78-79	0.009253463424973754	0.0	0.0	0.3997734656770694	0.0
80-81	0.010266906448610821	0.0	0.0	0.40042922292765804	0.0
82-83	0.010419254092686982	0.0	0.0	0.4010982277994708	0.0
84-85	0.01064446365349522	0.0	0.0	0.401383051655787	0.0
86-87	0.010876297024915464	0.0	0.0	0.4014956564361912	0.0
88	0.010916039888587506	0.0	0.0	0.4015089040574152	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14070	0.0	52.467876	1
GTATCAA	26130	0.0	38.491	1
ATCAACG	32260	0.0	30.644304	3
CAACGCA	32605	0.0	30.346395	5
TCAACGC	32770	0.0	30.206701	4
TATCAAC	33200	0.0	30.035568	2
AACGCAG	33660	0.0	29.541075	6
ACGCAGA	37770	0.0	26.23554	7
CGCAGAG	38105	0.0	25.982344	8
GCAGAGT	43815	0.0	22.488474	9
TACATGG	29285	0.0	22.407318	2
TGGTATC	5725	0.0	21.753756	2
GTACATG	30925	0.0	21.732834	1
ACATGGG	29285	0.0	21.556633	3
GTGGTAT	6120	0.0	21.472456	1
GAGTACT	28115	0.0	20.76015	12-13
CAGAGTA	42365	0.0	20.272327	10-11
AGAGTAC	41150	0.0	19.42	10-11
GTACTTT	30675	0.0	19.047844	14-15
CATGGGG	18350	0.0	18.582037	4
>>END_MODULE