FastQCFastQC Report
Fri 27 May 2016
SRR2138919_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138919_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11850835
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161700.052.2207371
GTATCAA295100.037.912881
TCAACGC358350.030.5299684
ATCAACG359450.030.4242523
CAACGCA367200.029.7339945
TATCAAC376200.029.2111932
AACGCAG376000.029.1354226
ACGCAGA433650.025.251937
CGCAGAG439250.024.9098728
GTGGTAT78350.024.5325411
TGGTATC74550.023.9456182
GTACATG310350.021.2853971
GCAGAGT512650.021.2485169
AGACCGA41850.021.0155888-89
TACATGG305750.020.9408472
ACATGGG306750.020.2383423
CGAGACC42450.020.1700986-87
GAGTACT357700.020.06203712-13
GACCGAG42900.020.05466790-91
ACCGAGG45500.019.8814890-91
Produced by FastQC (version 0.11.5)