##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138919_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11850835
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67882896015344	33.0	33.0	33.0	33.0	33.0
2	32.682993898742154	33.0	33.0	33.0	33.0	33.0
3	32.69479585193786	33.0	33.0	33.0	33.0	33.0
4	36.6173793660953	37.0	37.0	37.0	37.0	37.0
5	36.62692249111561	37.0	37.0	37.0	37.0	37.0
6	36.57052384916337	37.0	37.0	37.0	37.0	37.0
7	36.54506589620056	37.0	37.0	37.0	37.0	37.0
8	36.54221116064817	37.0	37.0	37.0	37.0	37.0
9	36.58355736114797	37.0	37.0	37.0	37.0	37.0
10-11	36.564039706906726	37.0	37.0	37.0	37.0	37.0
12-13	36.55516547990078	37.0	37.0	37.0	37.0	37.0
14-15	38.643959813802155	40.0	37.0	40.0	37.0	40.0
16-17	38.581345322924506	40.0	37.0	40.0	37.0	40.0
18-19	38.59959859368559	40.0	37.0	40.0	37.0	40.0
20-21	38.61259016405332	40.0	37.0	40.0	37.0	40.0
22-23	38.60969446677838	40.0	37.0	40.0	37.0	40.0
24-25	38.6122929927427	40.0	37.0	40.0	37.0	40.0
26-27	38.527664475163434	40.0	37.0	40.0	37.0	40.0
28-29	38.41367885669558	40.0	37.0	40.0	37.0	40.0
30-31	38.28202832931835	40.0	37.0	40.0	37.0	40.0
32-33	38.171241063398654	40.0	37.0	40.0	37.0	40.0
34-35	38.146498254207636	40.0	37.0	40.0	37.0	40.0
36-37	38.128031072670574	40.0	37.0	40.0	37.0	40.0
38-39	38.084839782671395	40.0	37.0	40.0	37.0	40.0
40-41	38.056941748553	40.0	37.0	40.0	37.0	40.0
42-43	38.00093345361197	40.0	37.0	40.0	33.0	40.0
44-45	37.90347146105435	40.0	37.0	40.0	33.0	40.0
46-47	37.79836189082158	40.0	37.0	40.0	33.0	40.0
48-49	37.73910307385143	40.0	37.0	40.0	33.0	40.0
50-51	37.718395939573846	40.0	37.0	40.0	33.0	40.0
52-53	37.658828521953154	40.0	37.0	40.0	33.0	40.0
54-55	37.547460160023704	40.0	37.0	40.0	33.0	40.0
56-57	37.37507979551198	40.0	37.0	40.0	33.0	40.0
58-59	37.190669139503626	38.5	37.0	40.0	33.0	40.0
60-61	37.02345421861655	37.0	37.0	40.0	33.0	40.0
62-63	36.88549701932457	37.0	37.0	40.0	33.0	40.0
64-65	36.718002090570636	37.0	37.0	40.0	33.0	40.0
66-67	36.50795835451278	37.0	37.0	40.0	33.0	40.0
68-69	36.30372314802999	37.0	37.0	40.0	33.0	40.0
70-71	36.076081659782965	37.0	37.0	38.5	33.0	40.0
72-73	35.782343614605765	37.0	35.0	37.0	33.0	40.0
74-75	35.41595169581591	37.0	33.0	37.0	33.0	40.0
76-77	33.34736434162498	35.0	33.0	37.0	27.0	37.0
78-79	34.971664109376874	37.0	33.0	37.0	33.0	37.0
80-81	35.17204197612676	37.0	33.0	37.0	33.0	37.0
82-83	34.96379063938045	37.0	33.0	37.0	33.0	37.0
84-85	34.80468714662806	37.0	33.0	37.0	33.0	37.0
86-87	34.69455710508245	37.0	33.0	37.0	33.0	37.0
88-89	34.57832110935858	37.0	33.0	37.0	33.0	37.0
90-91	34.501458649394074	37.0	33.0	37.0	33.0	37.0
92-93	34.457424782735785	37.0	33.0	37.0	33.0	37.0
94-95	34.408992176472736	37.0	33.0	37.0	33.0	37.0
96-97	34.42316103488774	37.0	33.0	37.0	33.0	37.0
98-99	34.472939989218844	37.0	33.0	37.0	33.0	37.0
100	34.45529687996397	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	2.0
18	5.0
19	17.0
20	75.0
21	333.0
22	1292.0
23	3831.0
24	9479.0
25	19366.0
26	34827.0
27	56622.0
28	85103.0
29	121373.0
30	167220.0
31	225573.0
32	301330.0
33	406681.0
34	567522.0
35	847654.0
36	1516110.0
37	3599000.0
38	3801720.0
39	85699.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.30235548803101	20.710312817620025	14.788333480299068	23.198998214049897
2	14.304969417695782	20.87585282285081	43.042124968060804	21.777052791392602
3	18.21103747722521	26.11965210182853	30.65188547226758	25.017424948678674
4	12.297741045251241	17.863019778775083	39.603462540825184	30.235776635148493
5	13.0891367570302	37.74822617984302	35.98992813586553	13.172708927261242
6	29.555149489466352	38.317696601125576	18.826833720999407	13.300320188408666
7	26.03819055788052	32.8480651363385	23.640511407002123	17.47323289877886
8	23.58713120214736	37.819934207167684	21.386552086836076	17.206382503848886
9	25.069322119496224	17.264960654671167	21.996770691685438	35.668946534147175
10-11	23.832860743149137	27.20158415981943	29.26357993066221	19.701975166369227
12-13	24.45629038082933	24.695262569194064	29.727906475612127	21.120540574364483
14-15	21.570319728525458	26.15879387401816	27.466288240448876	24.8045981570075
16-17	20.936111252920153	29.270241295233628	28.28768605756472	21.5059613942815
18-19	20.928259485513045	28.241157690576234	30.307720932744402	20.52286189116632
20-21	21.582442990455075	27.51599663728333	30.342235531510703	20.559324840750897
22-23	21.27856332877421	27.338416640765438	30.30538074044118	21.077639290019167
24-25	21.051043844798727	27.488479964765272	30.389550351245752	21.070925839190245
26-27	21.000308980734655	27.71513284968427	30.299273952265494	20.985284217315577
28-29	20.98093983734275	27.801120013413637	30.24160808230804	20.976332066935573
30-31	21.260712680124094	27.643300826784145	30.16583277017916	20.930153722912596
32-33	21.076394590412864	27.841903817147966	30.03511762584874	21.046583966590433
34-35	21.165684706984862	27.85657537206297	30.009373887227024	20.968366033725143
36-37	21.1675782269199	27.782706265491825	30.039476124923898	21.010239382664373
38-39	21.031184286705894	28.001441066160677	30.010057991030433	20.957316656102993
40-41	21.29080434790962	27.904036533439104	29.79634810772685	21.008811010924425
42-43	21.213060705741306	27.985844155571915	29.585394853864642	21.215700284822134
44-45	21.286552308865843	28.144718586432955	29.359341218828632	21.209387885872566
46-47	21.37805142509244	28.16879041603359	29.030889869990688	21.42226828888328
48-49	21.32332035603565	28.316318803414024	28.95207075368125	21.40829008686907
50-51	21.331891899976736	28.451834399168774	28.834662959080244	21.38161074177425
52-53	21.50871759833122	28.320460145036613	28.66775044906725	21.503071807564925
54-55	21.505980538014114	28.22211312197179	28.7259008033467	21.546005536667394
56-57	21.458552935104443	28.35788148384965	28.716872830637037	21.46669275040887
58-59	21.410338927881345	28.37875616297541	28.658272677000635	21.55263223214261
60-61	21.5119301796729	28.29704186495935	28.7422242130849	21.448803742282855
62-63	21.43117074722844	28.41286441497686	28.636797845784322	21.519166992010376
64-65	21.447824257849575	28.335050834303104	28.614725562718142	21.602399345129175
66-67	21.5587882148317	28.358897162511397	28.52899722529511	21.553317397361788
68-69	21.476425891849985	28.442584313568492	28.50913931603397	21.571850478547557
70-71	21.527571631089746	28.164564767451814	28.499388427890615	21.80847517356782
72-73	21.6152824114815	28.239548143473993	28.35551401584744	21.789655429197072
74-75	21.398832098136563	28.416760142147822	28.449263852108537	21.73514390760708
76-77	21.62031616240682	28.22911981903254	28.340058766797295	21.810505251763345
78-79	21.65142986280002	28.238024974942892	28.20781895172106	21.90272621053603
80-81	21.712143388678744	28.290333298165493	28.21161327281032	21.785910040345442
82-83	21.79762532731384	28.15413692302206	28.251334292201076	21.796903457463024
84-85	21.78979522020821	28.11115570724069	28.23865302977263	21.860396042778476
86-87	21.664606523853234	28.195992552451145	28.268722828654326	21.8706780950413
88-89	21.84118584969255	28.18957752477208	28.224249370929865	21.7449872546055
90-91	21.890355028945407	28.13844565886827	28.246903164703035	21.72429614748328
92-93	21.84912790780734	27.96922278464582	28.194797121367166	21.98685218617967
94-95	21.819152170122603	28.120815716113334	28.234201655718415	21.82583045804565
96-97	22.007199083609063	28.119332423662456	28.31889180238064	21.55457669034784
98-99	22.0495998743427	28.92426617964198	27.446346511877056	21.57978743413826
100	20.290411046185948	28.544011206891213	28.482883916111145	22.682693830811694
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2911.0
1	2576.0
2	3600.0
3	5357.5
4	7333.5
5	12167.5
6	16427.0
7	19756.5
8	22313.0
9	22284.0
10	20263.5
11	18007.0
12	19075.0
13	24448.0
14	37217.5
15	55397.5
16	70421.0
17	79069.5
18	81163.5
19	76784.0
20	68826.5
21	60453.5
22	54698.0
23	55550.0
24	62670.0
25	76000.5
26	95899.0
27	118423.0
28	141834.5
29	167943.5
30	193464.0
31	218769.0
32	248914.5
33	280097.5
34	310875.0
35	338720.5
36	368966.0
37	398815.0
38	417861.0
39	432102.0
40	451734.0
41	471578.5
42	483136.5
43	492487.0
44	499687.5
45	505580.0
46	507398.5
47	501702.5
48	491785.5
49	473063.0
50	447300.5
51	417900.5
52	382575.0
53	344846.0
54	306890.5
55	269339.0
56	233647.5
57	199504.5
58	170752.0
59	146617.0
60	120818.5
61	96831.5
62	75781.0
63	57457.5
64	42970.5
65	31329.0
66	23466.5
67	18346.0
68	13907.0
69	10067.5
70	7404.0
71	5421.0
72	3865.0
73	2771.0
74	1988.5
75	1406.0
76	935.5
77	607.5
78	442.5
79	325.5
80	229.0
81	153.0
82	102.5
83	66.5
84	46.5
85	39.0
86	34.5
87	28.5
88	20.5
89	13.5
90	9.5
91	6.5
92	4.5
93	3.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001611700778890264
3	9.2820463705722E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	8.860135171909828E-5
12-13	0.007767385167374281
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.219586078015842E-6
24-25	2.194568965131591E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	5.594295107373595E-4
36-37	0.026025265990530542
38-39	0.12465787780278177
40-41	0.14629002959736262
42-43	0.032601217950368314
44-45	0.002335659117421398
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.007280230757965416
64-65	0.07378390358307609
66-67	0.17934500947961338
68-69	0.18689563381319163
70-71	0.07046334968378148
72-73	0.002439143147916649
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	164.0
20-21	799.0
22-23	1652.0
24-25	3383.0
26-27	4483.0
28-29	7654.0
30-31	14368.0
32-33	15774.0
34-35	20292.0
36-37	22167.0
38-39	24393.0
40-41	33714.0
42-43	43821.0
44-45	52061.0
46-47	54655.0
48-49	43847.0
50-51	36899.0
52-53	42549.0
54-55	42764.0
56-57	40301.0
58-59	32309.0
60-61	57623.0
62-63	46497.0
64-65	69051.0
66-67	93943.0
68-69	88449.0
70-71	90734.0
72-73	90614.0
74-75	89394.0
76-77	81967.0
78-79	91196.0
80-81	100411.0
82-83	94624.0
84-85	107287.0
86-87	118589.0
88-89	126075.0
90-91	133118.0
92-93	139114.0
94-95	155674.0
96-97	428481.0
98-99	69793.0
100-101	9040152.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.51511293620092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.71487477445652	69.81857935713887
2	8.723355661643154	14.04723932559693
3	2.4350153396347802	5.881666052162279
4	0.9421449727914539	3.0342763554631147
5	0.470711956755886	1.8949713179310146
6	0.24720926384887357	1.1942449078600288
7	0.1401641364147073	0.7899731891124662
8	0.08457448720061404	0.5447619510782976
9	0.06527651024096769	0.47301710347193315
>10	0.17371151914804675	2.0587243313195427
>50	0.0022345550342912825	0.11978264814616436
>100	6.291147317475497E-4	0.08489533775422665
>500	7.816818558956241E-5	0.04182088798547379
>1k	1.9539913364946135E-5	0.016047234979690645
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.687644794649491E-5	0.0
2	0.0	4.219111986623727E-5	0.0	1.687644794649491E-5	0.0
3	0.0	5.062934383948473E-5	0.0	1.687644794649491E-5	0.0
4	0.0	1.4344980754520673E-4	0.0	3.375289589298982E-5	8.438223973247454E-6
5	0.0	1.518880315184542E-4	0.0	3.375289589298982E-5	8.438223973247454E-6
6	0.0	1.518880315184542E-4	0.0	4.219111986623727E-5	8.438223973247454E-6
7	0.0	1.518880315184542E-4	0.0	5.062934383948473E-5	8.438223973247454E-6
8	8.438223973247454E-6	1.7720270343819656E-4	0.0	5.062934383948473E-5	8.438223973247454E-6
9	8.438223973247454E-6	2.8689961509041346E-4	0.0	5.9067567812732185E-5	8.438223973247454E-6
10-11	1.265733595987118E-5	2.953378390636609E-4	0.0	5.9067567812732185E-5	8.438223973247454E-6
12-13	1.687644794649491E-5	3.122142870101558E-4	0.0	8.860135171909826E-5	8.438223973247454E-6
14-15	1.687644794649491E-5	4.345685346222439E-4	0.0	9.2820463705722E-5	1.265733595987118E-5
16-17	1.687644794649491E-5	4.5566409455536256E-4	0.0	1.0125868767896946E-4	1.687644794649491E-5
18-19	1.687644794649491E-5	5.189507743547185E-4	0.0	1.1391602363884064E-4	1.687644794649491E-5
20-21	1.687644794649491E-5	5.991139021005692E-4	0.0	1.5188803151845417E-4	1.687644794649491E-5
22-23	1.687644794649491E-5	6.877152538196675E-4	0.0	3.2065251098340326E-4	2.531467191974236E-5
24-25	1.687644794649491E-5	7.594401575922709E-4	0.0	7.299063736859048E-4	3.375289589298982E-5
26-27	1.687644794649491E-5	7.763166055387658E-4	0.0	0.0017256168025291045	3.375289589298982E-5
28-29	2.5314671919742365E-5	7.763166055387658E-4	0.0	0.0049616756962695035	3.375289589298982E-5
30-31	2.5314671919742365E-5	8.775752932177353E-4	0.0	0.005366710446985381	3.375289589298982E-5
32-33	2.9533783906366093E-5	8.81794405204359E-4	0.0	0.0064341457796011844	4.219111986623727E-5
34-35	3.375289589298982E-5	0.0010252442127495656	0.0	0.0076408118077755695	4.219111986623727E-5
36-37	3.375289589298982E-5	0.0011729131322813962	0.0	0.009020461427401528	5.062934383948473E-5
38-39	3.375289589298982E-5	0.0012193233641342573	0.0	0.011754445994733704	5.062934383948473E-5
40-41	6.750579178597963E-5	0.0014007451795590776	0.0	0.01453484079391874	5.062934383948473E-5
42-43	7.59440157592271E-5	0.0014218407394921962	0.0	0.017585258760247698	5.062934383948473E-5
44-45	8.438223973247455E-5	0.0016201390028635113	0.0	0.020956329237560054	5.062934383948473E-5
46-47	9.2820463705722E-5	0.001797341706301708	0.0	0.025053086976571694	5.062934383948473E-5
48-49	9.2820463705722E-5	0.0018015608182883316	0.0	0.03019618448826602	5.062934383948473E-5
50-51	9.2820463705722E-5	0.002008297305632894	0.0	0.03460937562627443	5.062934383948473E-5
52-53	9.2820463705722E-5	0.0020336119775526368	0.0	0.049258132443832016	5.4848455826108454E-5
54-55	9.703957569234573E-5	0.002063145761459003	0.0	0.06705856591539752	5.9067567812732185E-5
56-57	1.0125868767896946E-4	0.002092679545365369	0.0	0.08358904667898928	5.9067567812732185E-5
58-59	1.0547779966559319E-4	0.0021011177693386163	0.0	0.16251597461275935	5.9067567812732185E-5
60-61	1.0969691165221691E-4	0.0021264324412583586	0.0	0.23418181081754996	5.9067567812732185E-5
62-63	1.0969691165221691E-4	0.002164404449137972	0.0	0.23710987453626686	5.9067567812732185E-5
64-65	1.0969691165221691E-4	0.0023880173844290295	0.0	0.23968353284810734	5.9067567812732185E-5
66-67	1.0969691165221691E-4	0.0024048938323755244	0.0	0.24352714386792154	5.9067567812732185E-5
68-69	1.1391602363884064E-4	0.0024555231762150094	0.0	0.24841709466041845	5.9067567812732185E-5
70-71	1.1813513562546437E-4	0.002493495184094623	0.0	0.2522016381124199	5.9067567812732185E-5
72-73	1.1813513562546437E-4	0.0025145907440277415	0.0	0.2555220792458928	5.9067567812732185E-5
74-75	1.1813513562546437E-4	0.002586315647800345	0.0	0.2580113553180008	5.9067567812732185E-5
76-77	1.1813513562546437E-4	0.002721327231372304	0.0	0.26064830030964065	5.9067567812732185E-5
78-79	1.223542476120881E-4	0.0027930521351449073	0.0	0.26234860244025004	5.9067567812732185E-5
80-81	1.2657335959871183E-4	0.002801490359118155	0.0	0.26308694703790914	5.9067567812732185E-5
82-83	1.2657335959871183E-4	0.002801490359118155	0.0	0.2635637066923976	5.9067567812732185E-5
84-85	1.2657335959871183E-4	0.00281836680706465	0.0	0.2638295107475549	5.9067567812732185E-5
86-87	1.2657335959871183E-4	0.002877434374877382	0.0	0.263951864995167	5.9067567812732185E-5
88	1.2657335959871183E-4	0.003004007734476094	0.0	0.2639813987790734	5.9067567812732185E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16170	0.0	52.220737	1
GTATCAA	29510	0.0	37.91288	1
TCAACGC	35835	0.0	30.529968	4
ATCAACG	35945	0.0	30.424252	3
CAACGCA	36720	0.0	29.733994	5
TATCAAC	37620	0.0	29.211193	2
AACGCAG	37600	0.0	29.135422	6
ACGCAGA	43365	0.0	25.25193	7
CGCAGAG	43925	0.0	24.909872	8
GTGGTAT	7835	0.0	24.532541	1
TGGTATC	7455	0.0	23.945618	2
GTACATG	31035	0.0	21.285397	1
GCAGAGT	51265	0.0	21.248516	9
AGACCGA	4185	0.0	21.01558	88-89
TACATGG	30575	0.0	20.940847	2
ACATGGG	30675	0.0	20.238342	3
CGAGACC	4245	0.0	20.17009	86-87
GAGTACT	35770	0.0	20.062037	12-13
GACCGAG	4290	0.0	20.054667	90-91
ACCGAGG	4550	0.0	19.88148	90-91
>>END_MODULE