FastQCFastQC Report
Fri 27 May 2016
SRR2138918_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138918_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9590358
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135450.049.6305851
ATCTCGT53500.045.78890294
GTATCAA240950.036.765531
TATCTCG68800.034.03397494
ATCAACG289900.029.699673
CAACGCA297850.028.965345
TCAACGC302200.028.5772534
AACGCAG306050.028.264626
TATCAAC311250.028.1393852
ACGCAGA340900.025.3111957
CGCAGAG343550.025.1413388
CGCTATC103150.025.10605494
TATGCCG22000.024.40671586-87
TCGTATG25100.022.92006584-85
TACATGG265000.022.0775412
GTACTAG29650.021.6140751
TGCCGTC25000.021.56736688-89
ACATGGG262750.021.5525743
GCAGAGT399850.021.4378059
CTCGTAT25650.020.9227982-83
Produced by FastQC (version 0.11.5)