FastQCFastQC Report
Fri 27 May 2016
SRR2138917_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138917_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8550608
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165250.049.176681
GTATCAA299650.037.1404721
ATCAACG368200.029.728063
CAACGCA377600.028.9992895
TCAACGC379950.028.8647674
TATCAAC383950.028.808432
AACGCAG382650.028.7416936
ACGCAGA425700.025.6950367
CGCAGAG429950.025.4311398
GTACTAG28700.022.2603341
GCAGAGT496500.021.953769
TACATGG306150.021.53572
GTACATG322850.021.2396551
ACATGGG306400.021.0869663
GTGGTAT78400.020.9697651
TGGTATC76050.020.6656682
GAGTACT330650.020.26916112-13
CAGAGTA479200.019.76415310-11
AGAGTAC467700.019.16179810-11
CATGGGG220400.019.0151944
Produced by FastQC (version 0.11.5)