##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138917_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8550608
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69807573917551	33.0	33.0	33.0	33.0	33.0
2	32.6956078444948	33.0	33.0	33.0	33.0	33.0
3	32.70358821267447	33.0	33.0	33.0	33.0	33.0
4	36.6308690563291	37.0	37.0	37.0	37.0	37.0
5	36.646921131222484	37.0	37.0	37.0	37.0	37.0
6	36.59140402647391	37.0	37.0	37.0	37.0	37.0
7	36.5670430687502	37.0	37.0	37.0	37.0	37.0
8	36.56790101943628	37.0	37.0	37.0	37.0	37.0
9	36.60707893520554	37.0	37.0	37.0	37.0	37.0
10-11	36.586231119471265	37.0	37.0	37.0	37.0	37.0
12-13	36.575001450189276	37.0	37.0	37.0	37.0	37.0
14-15	38.66707367476091	40.0	37.0	40.0	37.0	40.0
16-17	38.60717436701577	40.0	37.0	40.0	37.0	40.0
18-19	38.64031663011566	40.0	37.0	40.0	37.0	40.0
20-21	38.65840778725972	40.0	37.0	40.0	37.0	40.0
22-23	38.65601686476654	40.0	37.0	40.0	37.0	40.0
24-25	38.650301776917324	40.0	37.0	40.0	37.0	40.0
26-27	38.562223847295925	40.0	37.0	40.0	37.0	40.0
28-29	38.445058679631444	40.0	37.0	40.0	37.0	40.0
30-31	38.29582846019382	40.0	37.0	40.0	37.0	40.0
32-33	38.17808169975406	40.0	37.0	40.0	37.0	40.0
34-35	38.15368344690058	40.0	37.0	40.0	37.0	40.0
36-37	38.136471103511425	40.0	37.0	40.0	37.0	40.0
38-39	38.09203970434695	40.0	37.0	40.0	37.0	40.0
40-41	38.060015931253915	40.0	37.0	40.0	37.0	40.0
42-43	37.998418889734225	40.0	37.0	40.0	33.0	40.0
44-45	37.893365813329815	40.0	37.0	40.0	33.0	40.0
46-47	37.78041817522721	40.0	37.0	40.0	33.0	40.0
48-49	37.71058031511474	40.0	37.0	40.0	33.0	40.0
50-51	37.682203805773725	40.0	37.0	40.0	33.0	40.0
52-53	37.619009606582665	40.0	37.0	40.0	33.0	40.0
54-55	37.50188578750759	40.0	37.0	40.0	33.0	40.0
56-57	37.31713492838995	38.5	37.0	40.0	33.0	40.0
58-59	37.12107375026243	37.0	37.0	40.0	33.0	40.0
60-61	36.93924690490128	37.0	37.0	40.0	33.0	40.0
62-63	36.78896940964475	37.0	37.0	40.0	33.0	40.0
64-65	36.60994204282888	37.0	37.0	40.0	33.0	40.0
66-67	36.396941171640904	37.0	37.0	40.0	33.0	40.0
68-69	36.193488612643684	37.0	37.0	40.0	33.0	40.0
70-71	35.97064658087943	37.0	37.0	37.0	33.0	40.0
72-73	35.68506049237338	37.0	33.0	37.0	33.0	40.0
74-75	35.33631021347453	37.0	33.0	37.0	33.0	40.0
76-77	33.22523475403702	35.0	33.0	37.0	27.0	37.0
78-79	34.902358935431465	37.0	33.0	37.0	33.0	37.0
80-81	35.133464051027055	37.0	33.0	37.0	33.0	37.0
82-83	34.94987997095789	37.0	33.0	37.0	33.0	37.0
84-85	34.81075208201635	37.0	33.0	37.0	33.0	37.0
86-87	34.712400495768804	37.0	33.0	37.0	33.0	37.0
88-89	34.60890054573059	37.0	33.0	37.0	33.0	37.0
90-91	34.538979875858125	37.0	33.0	37.0	33.0	37.0
92-93	34.495833752359644	37.0	33.0	37.0	33.0	37.0
94-95	34.46009372979095	37.0	33.0	37.0	33.0	37.0
96-97	34.478662691584276	37.0	33.0	37.0	33.0	37.0
98-99	34.51112081470495	37.0	33.0	37.0	33.0	37.0
100	34.46649081282395	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	2.0
18	9.0
19	4.0
20	51.0
21	191.0
22	818.0
23	2537.0
24	6083.0
25	12622.0
26	23019.0
27	37475.0
28	57532.0
29	82295.0
30	114708.0
31	155082.0
32	208940.0
33	285461.0
34	403532.0
35	609975.0
36	1132427.0
37	2691010.0
38	2655161.0
39	71672.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.265836534665134	20.403168991023797	14.352839002793718	22.978155471517347
2	14.790747686646824	20.710284011387916	42.227026898062995	22.271941403902265
3	17.995112386344154	26.59186016784331	30.73178307846215	24.681244367350384
4	12.178420528692229	17.85851953451731	39.208510084896886	30.754549851893575
5	12.950880218108466	38.111512070252786	35.63511507017981	13.302492641458945
6	29.637026981005327	38.02740109241354	18.811387447535893	13.524184479045232
7	26.225737397855216	33.1248491335353	23.376173951606717	17.273239517002768
8	24.84816284409249	36.240054508404555	21.032668086292812	17.879114561210148
9	25.99340304221641	16.22525556077416	21.45101260635501	36.33032879065442
10-11	24.27860935655649	26.73186908572413	28.832153708925556	20.15736784879382
12-13	25.30564912280702	24.394345029239766	29.047730994152047	21.252274853801172
14-15	22.33423050150352	25.492649177695903	27.37798294577415	24.795137375026428
16-17	21.26035949724277	28.45644426688722	28.09178598761632	22.19141024825369
18-19	21.0791443134804	27.399934601141812	30.44960077692721	21.07132030845058
20-21	22.060218552329115	26.64067749233804	30.45013335703605	20.848970598296795
22-23	21.792867764275	26.578001568992203	30.255694029910973	21.373436636821815
24-25	21.54688854699419	26.647294020174332	30.54049285950019	21.265324573331284
26-27	21.415509285807573	26.968708001106627	30.21364987732988	21.402132835755918
28-29	21.1575406967662	27.29547045524966	30.234927260246415	21.312061587737723
30-31	21.643331612788074	26.940232385602748	30.08063890531808	21.3357970962911
32-33	21.42947203253534	27.16059992265638	29.95761384068661	21.452314204121667
34-35	21.59295813474894	27.231368201632506	29.992926882123054	21.1827467814955
36-37	21.599806198967677	27.131581838734682	29.91385863956058	21.35475332273706
38-39	21.449328468182944	27.25658354092857	29.983813503707797	21.310274487180685
40-41	21.696494438918744	27.099498005045756	29.829334921213263	21.374672634822232
42-43	21.772986162749667	27.310308764485956	29.29014234631867	21.626562726445705
44-45	21.697105876907038	27.708854217114304	28.975518815332517	21.618521090646137
46-47	21.933201316879526	27.58366773737554	28.490941063789816	21.992189881955117
48-49	22.089269213112367	27.720801915130377	28.303926747469703	21.886002124287554
50-51	22.01644966627006	27.714532637291523	28.18858323952179	22.080434456916624
52-53	22.073208663226193	27.804280078602734	27.925019838569465	22.19749141960161
54-55	22.10495481788098	27.69575961670639	27.973639090617425	22.225646474795205
56-57	22.24671512063722	27.694863328794312	27.844258552873374	22.214162997695095
58-59	22.17982972432685	27.70287812937362	28.042108419135893	22.07518372716364
60-61	22.249455636741757	27.848513830312786	27.892522929988495	22.00950760295696
62-63	22.246448539048362	27.67153399627184	27.77229176433378	22.309725700346018
64-65	22.17009185224344	27.696073172845786	27.75228472049988	22.38155025441089
66-67	22.239123474312574	27.779572119227026	27.569477511990815	22.41182689446959
68-69	22.203124488002977	27.71803427862744	27.751286540228676	22.327554693140904
70-71	22.365220628729567	27.31946076176037	27.636014052572726	22.67930455693733
72-73	22.37920533459083	27.51525471637743	27.649967781245095	22.455572167786652
74-75	22.300467213611842	27.63279587858497	27.600463497563776	22.466273410239413
76-77	22.41307256962837	27.449420112074492	27.55682483481558	22.580682483481556
78-79	22.370970575624067	27.4397334706222	27.596461282437467	22.592834671316265
80-81	22.222383852122736	27.476102270192083	27.66467705778201	22.636836819903166
82-83	22.601546273203592	27.324915833359007	27.725254577669382	22.34828331576802
84-85	22.81490213875914	27.35600988019082	27.387319630656375	22.441768350393662
86-87	22.42188249018751	27.454966476893905	27.623720058461426	22.49943097445716
88-89	22.664008568277012	27.195054123038716	27.594591386285156	22.546345922399116
90-91	22.728623950514947	27.2030236858951	27.588023623165835	22.480328740424113
92-93	22.823807153592774	27.153377922940642	27.576655895259655	22.446159028206925
94-95	22.473166034534966	27.17256239654875	27.715869860345983	22.6384017085703
96-97	22.556024517886687	27.304443853140683	27.75622257042274	22.383309058549894
98-99	23.669655629397873	29.235070412251297	27.91347593923852	19.181798019112314
100	22.736178582311506	30.00619517833803	21.620380913526173	25.63724532582429
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2291.0
1	2230.5
2	3981.0
3	6158.5
4	8571.5
5	14921.5
6	20160.0
7	23163.5
8	24324.0
9	22419.5
10	19113.5
11	16346.0
12	18090.5
13	25051.5
14	40076.0
15	60531.0
16	76826.0
17	85021.5
18	85231.5
19	79131.5
20	69251.5
21	58351.0
22	50063.5
23	47655.5
24	51082.0
25	58344.5
26	69261.0
27	82316.0
28	94043.0
29	109088.0
30	124710.5
31	140833.0
32	162293.5
33	183565.5
34	205995.5
35	228056.5
36	251152.5
37	278081.5
38	292879.5
39	298009.0
40	307340.0
41	312701.5
42	315549.5
43	325341.5
44	338764.0
45	354294.5
46	368405.5
47	378443.5
48	380294.0
49	371220.0
50	357736.0
51	344486.5
52	329302.0
53	309098.5
54	286664.5
55	261784.0
56	234355.5
57	200518.5
58	167996.0
59	142275.5
60	114003.0
61	87072.0
62	66820.5
63	51120.5
64	37517.0
65	26236.5
66	18767.5
67	14439.5
68	10879.5
69	7947.5
70	5880.0
71	3730.0
72	2618.5
73	1842.0
74	1209.0
75	870.0
76	545.0
77	338.0
78	208.5
79	125.5
80	87.5
81	67.0
82	55.5
83	35.5
84	21.5
85	15.0
86	13.5
87	11.0
88	8.0
89	7.5
90	5.0
91	2.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0016490055444010531
3	1.637310469618067E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.847537391493096E-5
12-13	0.0071106054680556054
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.1696675266842102E-5
24-25	3.100206573198363E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	6.191519622989525E-4
36-37	0.025201996429203975
38-39	0.11953411173524489
40-41	0.14090958733055692
42-43	0.03105353183195365
44-45	0.002291902142849262
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.007297908956000416
64-65	0.07386869512909244
66-67	0.18130027953620506
68-69	0.18825161122989933
70-71	0.07018507695648274
72-73	0.0022242936669910713
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	131.0
20-21	724.0
22-23	1453.0
24-25	2512.0
26-27	4194.0
28-29	9587.0
30-31	20050.0
32-33	25062.0
34-35	32987.0
36-37	44540.0
38-39	53532.0
40-41	60134.0
42-43	71780.0
44-45	84954.0
46-47	81752.0
48-49	69125.0
50-51	57907.0
52-53	52630.0
54-55	55705.0
56-57	56777.0
58-59	56610.0
60-61	54352.0
62-63	59190.0
64-65	61833.0
66-67	71195.0
68-69	68092.0
70-71	70975.0
72-73	67759.0
74-75	66466.0
76-77	60313.0
78-79	64846.0
80-81	68337.0
82-83	66996.0
84-85	75346.0
86-87	83568.0
88-89	90229.0
90-91	92396.0
92-93	99288.0
94-95	112650.0
96-97	285385.0
98-99	530084.0
100-101	5559162.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.34985883468062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.76561966154355	65.48192954027758
2	8.58218310983272	13.104969378582169
3	2.641687367233553	6.050773727209227
4	1.1926309472347751	3.64228817852986
5	0.6411964527454567	2.4477629326206785
6	0.3476337143010284	1.5925071007835527
7	0.2338922238777643	1.2500346792917774
8	0.14033092123328994	0.8571396821041898
9	0.1090129265723396	0.7490809399458178
>10	0.3379412796317891	3.8903886007656956
>50	0.005300518086698334	0.26866817500132734
>100	0.0020801306731485143	0.3119858551593413
>500	3.154802470896857E-4	0.17371987130346156
>1k	1.7526678682112403E-4	0.17875133842533875
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.3390149565972385E-5	0.0	0.0	0.0	0.0
3	2.3390149565972385E-5	0.0	0.0	0.0	0.0
4	2.3390149565972385E-5	0.0	0.0	0.0	0.0
5	4.678029913194477E-5	0.0	0.0	0.0	0.0
6	9.356059826388954E-5	0.0	0.0	0.0	0.0
7	1.520359721788205E-4	0.0	0.0	0.0	0.0
8	1.520359721788205E-4	0.0	0.0	0.0	0.0
9	1.520359721788205E-4	0.0	0.0	1.1695074782986192E-5	0.0
10-11	1.9296873391927216E-4	0.0	0.0	1.1695074782986192E-5	0.0
12-13	2.572916452256962E-4	0.0	0.0	1.1695074782986192E-5	0.0
14-15	4.3271776697048907E-4	0.0	0.0	1.1695074782986192E-5	0.0
16-17	4.794980661024338E-4	0.0	0.0	2.3390149565972385E-5	0.0
18-19	6.315340382812545E-4	0.0	0.0	4.0932761740451675E-5	0.0
20-21	7.017044869791715E-4	0.0	0.0	8.771306087239644E-5	0.0
22-23	7.484847861111163E-4	0.0	0.0	5.145832904513924E-4	0.0
24-25	9.706912069878539E-4	0.0	0.0	0.00164315800700956	0.0
26-27	9.882338191623333E-4	0.0	0.0	0.003964630351432319	0.0
28-29	0.0012513730017795226	0.0	0.0	0.011291594702973168	0.0
30-31	0.0013566286748263982	0.0	0.0	0.013326537715212767	0.0
32-33	0.0013917138991753567	0.0	0.0	0.017297015604036577	0.0
34-35	0.001532054796571191	0.0	0.0	0.022010130741580015	0.0
36-37	0.0017367186052734496	0.0	0.0	0.02957099658878059	0.0
38-39	0.0018653644278862976	0.0	0.0	0.04933567297202725	0.0
40-41	0.0018712119652777909	0.0	0.0	0.07078444012402393	0.0
42-43	0.0020466380870225835	0.0	0.0	0.08360224208617681	0.0
44-45	0.0022746920452908145	0.0	0.0	0.09854270012144166	0.0
46-47	0.0027717327235677273	0.0	0.0	0.11312061083843394	0.0
48-49	0.0029237686957465482	0.0	0.0	0.1277102166302092	0.0
50-51	0.0029237686957465482	0.0	0.0	0.14222965197328657	0.0
52-53	0.0029296162331380413	0.0	0.0	0.17589392473611234	0.0
54-55	0.0029588539200955067	0.0	0.0	0.21443504368344332	0.0
56-57	0.0031108898922743273	0.0	0.0	0.2514148701472457	0.0
58-59	0.0031108898922743273	0.0	0.0	0.27624351391152535	0.0
60-61	0.0031401275792317927	0.0	0.0	0.29665726694522776	0.0
62-63	0.0031869078783637373	0.0	0.0	0.31165620035440755	0.0
64-65	0.0032453832522786686	0.0	0.0	0.31641024825369146	0.0
66-67	0.0034851322853298853	0.0	0.0	0.3215794713077713	0.0
68-69	0.0035669978088107884	0.0	0.0	0.3270176810818599	0.0
70-71	0.0036196256453342266	0.0	0.0	0.33181266174288426	0.0
72-73	0.003707338706206623	0.0	0.0	0.33582991993084	0.0
74-75	0.0037775091549045402	0.0	0.0	0.3387010607900631	0.0
76-77	0.0037892042296875264	0.0	0.0	0.34164237209798415	0.0
78-79	0.003818441916644992	0.0	0.0	0.3433381579415171	0.0
80-81	0.004309635057530412	0.0	0.0	0.34396969197979843	0.0
82-83	0.004432433342751767	0.0	0.0	0.3444257998963348	0.0
84-85	0.004572774240147601	0.0	0.0	0.3446129210928626	0.0
86-87	0.004713115137543435	0.0	0.0	0.34475910952765	0.0
88	0.004736505287109408	0.0	0.0	0.3447941947519989	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16525	0.0	49.17668	1
GTATCAA	29965	0.0	37.140472	1
ATCAACG	36820	0.0	29.72806	3
CAACGCA	37760	0.0	28.999289	5
TCAACGC	37995	0.0	28.864767	4
TATCAAC	38395	0.0	28.80843	2
AACGCAG	38265	0.0	28.741693	6
ACGCAGA	42570	0.0	25.695036	7
CGCAGAG	42995	0.0	25.431139	8
GTACTAG	2870	0.0	22.260334	1
GCAGAGT	49650	0.0	21.95376	9
TACATGG	30615	0.0	21.5357	2
GTACATG	32285	0.0	21.239655	1
ACATGGG	30640	0.0	21.086966	3
GTGGTAT	7840	0.0	20.969765	1
TGGTATC	7605	0.0	20.665668	2
GAGTACT	33065	0.0	20.269161	12-13
CAGAGTA	47920	0.0	19.764153	10-11
AGAGTAC	46770	0.0	19.161798	10-11
CATGGGG	22040	0.0	19.015194	4
>>END_MODULE