FastQCFastQC Report
Fri 27 May 2016
SRR2138916_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138916_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8190889
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138350.052.0738641
GTATCAA246800.038.3288731
ATCAACG314700.029.6073423
TCAACGC319900.029.1660754
TATCAAC324700.028.9741952
CAACGCA322200.028.9044465
AACGCAG328900.028.4614776
ACGCAGA367600.025.4651287
CGCAGAG372200.025.1735328
CGCTACG2252.592289E-622.8624394
TACATGG269900.022.8021972
GCAGAGT419050.022.2769559
ACATGGG270750.022.2216843
GTACATG285500.022.0537131
GAGTACT289750.020.65356612-13
CATGGGG187850.020.641754
GTGGTAT59750.019.6638361
CAGAGTA418800.019.65426610-11
AGAGTAC398500.019.28384410-11
TGGTATC56950.019.1947982
Produced by FastQC (version 0.11.5)