##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138916_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8190889
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.708238507444065	33.0	33.0	33.0	33.0	33.0
2	32.709425924341055	33.0	33.0	33.0	33.0	33.0
3	32.71940103204915	33.0	33.0	33.0	33.0	33.0
4	36.6485199591888	37.0	37.0	37.0	37.0	37.0
5	36.66257215791839	37.0	37.0	37.0	37.0	37.0
6	36.605323915389405	37.0	37.0	37.0	37.0	37.0
7	36.58192242624702	37.0	37.0	37.0	37.0	37.0
8	36.5802859738424	37.0	37.0	37.0	37.0	37.0
9	36.61803950706694	37.0	37.0	37.0	37.0	37.0
10-11	36.59850878213586	37.0	37.0	37.0	37.0	37.0
12-13	36.58771642492042	37.0	37.0	37.0	37.0	37.0
14-15	38.706654857610694	40.0	37.0	40.0	37.0	40.0
16-17	38.6473908387722	40.0	37.0	40.0	37.0	40.0
18-19	38.67763456445326	40.0	37.0	40.0	37.0	40.0
20-21	38.69236803254742	40.0	37.0	40.0	37.0	40.0
22-23	38.68759305902439	40.0	37.0	40.0	37.0	40.0
24-25	38.68788765481921	40.0	37.0	40.0	37.0	40.0
26-27	38.60486814845859	40.0	37.0	40.0	37.0	40.0
28-29	38.4886467662188	40.0	37.0	40.0	37.0	40.0
30-31	38.351002957689715	40.0	37.0	40.0	37.0	40.0
32-33	38.23561341604079	40.0	37.0	40.0	37.0	40.0
34-35	38.21194346441504	40.0	37.0	40.0	37.0	40.0
36-37	38.1999057084066	40.0	37.0	40.0	37.0	40.0
38-39	38.15223798512297	40.0	37.0	40.0	37.0	40.0
40-41	38.125494348648665	40.0	37.0	40.0	37.0	40.0
42-43	38.06666391923664	40.0	37.0	40.0	35.0	40.0
44-45	37.962625050468105	40.0	37.0	40.0	33.0	40.0
46-47	37.858826677877175	40.0	37.0	40.0	33.0	40.0
48-49	37.787334908277884	40.0	37.0	40.0	33.0	40.0
50-51	37.75850601346843	40.0	37.0	40.0	33.0	40.0
52-53	37.69007576923207	40.0	37.0	40.0	33.0	40.0
54-55	37.56823345797595	40.0	37.0	40.0	33.0	40.0
56-57	37.387333773273454	40.0	37.0	40.0	33.0	40.0
58-59	37.1898437209053	37.0	37.0	40.0	33.0	40.0
60-61	37.01193776364158	37.0	37.0	40.0	33.0	40.0
62-63	36.86403145371957	37.0	37.0	40.0	33.0	40.0
64-65	36.6845846676556	37.0	37.0	40.0	33.0	40.0
66-67	36.47113059736802	37.0	37.0	40.0	33.0	40.0
68-69	36.26271498336651	37.0	37.0	40.0	33.0	40.0
70-71	36.0424039908292	37.0	37.0	38.5	33.0	40.0
72-73	35.76008293347269	37.0	35.0	37.0	33.0	40.0
74-75	35.41136604289158	37.0	33.0	37.0	33.0	40.0
76-77	33.305084180836424	35.0	33.0	37.0	27.0	37.0
78-79	34.98039877542045	37.0	33.0	37.0	33.0	37.0
80-81	35.203564561912216	37.0	33.0	37.0	33.0	37.0
82-83	35.00825476874927	37.0	33.0	37.0	33.0	37.0
84-85	34.863227729027294	37.0	33.0	37.0	33.0	37.0
86-87	34.766386203178314	37.0	33.0	37.0	33.0	37.0
88-89	34.66037027672867	37.0	33.0	37.0	33.0	37.0
90-91	34.589754881774354	37.0	33.0	37.0	33.0	37.0
92-93	34.55097297664814	37.0	33.0	37.0	33.0	37.0
94-95	34.50566345347718	37.0	33.0	37.0	33.0	37.0
96-97	34.51758165094811	37.0	33.0	37.0	33.0	37.0
98-99	34.54700509840467	37.0	33.0	37.0	33.0	37.0
100	34.5009785651194	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	5.0
19	9.0
20	46.0
21	198.0
22	781.0
23	2415.0
24	5857.0
25	12011.0
26	21642.0
27	35800.0
28	54003.0
29	77571.0
30	106080.0
31	142684.0
32	192716.0
33	264140.0
34	374045.0
35	565857.0
36	1049828.0
37	2569152.0
38	2642055.0
39	73992.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.72593984925688	20.62683557631358	14.558384878416113	23.088839696013423
2	14.586403872468729	20.69214957762079	42.70532689093933	22.01611965897115
3	17.875968700753084	26.739583643638255	30.610125813283766	24.77432184232489
4	12.189435382341939	17.7030866494573	39.54475998206788	30.56271798613288
5	12.89499833290379	38.124335465906086	35.79903231505151	13.181633886138611
6	29.480524031030086	38.36257578389843	18.68594483455947	13.470955350512014
7	26.073421334363093	33.0286614798467	23.622271037978905	17.275646147811305
8	24.340398215627143	36.86393503806485	21.107269308618392	17.68839743768961
9	25.68647920878918	16.789496231727714	21.731121005302352	35.79290355418075
10-11	24.24733861159927	26.96357151660483	28.9376135141449	19.851476357650995
12-13	25.027621017540092	24.480176051707865	29.196169478583318	21.296033452168725
14-15	22.09022732453095	25.722183513901847	27.31213913410376	24.87545002746344
16-17	21.189146867940707	28.68706925463158	28.069651535993223	22.05413234143449
18-19	21.086923531743622	27.757438783506892	30.203534683475752	20.952103001273734
20-21	22.1193126239518	27.00472878699752	30.137318201996315	20.738640387054367
22-23	21.75727558352898	26.73796177678231	30.10204156387866	21.40272107581005
24-25	21.39186994491035	26.88870806956895	30.36674652107007	21.352675464450623
26-27	21.33650367046394	27.14017148967508	30.147657175302424	21.37566766455856
28-29	21.255049013511602	27.421496032748806	30.099200377428943	21.22425457631065
30-31	21.64827301873777	27.16962258542452	29.933925802027538	21.248178593810174
32-33	21.450609959867432	27.424494663809252	29.81748112174977	21.30741425457355
34-35	21.45145450227033	27.416644281321595	29.92706568674908	21.20483552965899
36-37	21.61853762870679	27.222456333686107	29.87951447339609	21.279491564211014
38-39	21.3989986239488	27.514123234960213	29.83288182752308	21.2539963135679
40-41	21.637924186843726	27.4515865088855	29.659781945310304	21.25070735896047
42-43	21.639409506627956	27.55207600676496	29.332095419616817	21.476419066990267
44-45	21.649337334938675	27.829108463044022	29.006517003021422	21.515037198995877
46-47	21.808206446784503	27.59938725863495	28.65911781608353	21.93328847849702
48-49	21.92736235460353	27.79083101608808	28.50582170715363	21.775984922154763
50-51	21.69660378452523	27.96836547762928	28.3357208477117	21.999309890133784
52-53	21.912194657161738	27.90203178118082	28.104414582235325	22.081358979422117
54-55	22.04344137647101	27.724041941547505	28.14465895513661	22.087857726844877
56-57	22.00322653047926	27.82623204822814	28.08621721224344	22.08432420904916
58-59	21.899758199444133	27.798626865292086	28.30685041530994	21.994764519953844
60-61	21.95663751966874	27.952584710859117	28.075766679894187	22.015011089577957
62-63	22.092143615341143	27.860839265646586	27.993127792019152	22.05388932699312
64-65	21.91441457294867	27.948522747085075	27.941003284468785	22.19605939549747
66-67	22.114044570461687	28.005385488147994	27.62316421202668	22.257405729363644
68-69	21.986105083202595	27.926789820225835	27.91249741751306	22.174607679058514
70-71	22.084924973091617	27.45719511883565	27.99255812377746	22.465321784295277
72-73	22.235365721902113	27.559714639344236	27.910873497162687	22.294046141590968
74-75	21.930766267042905	27.84877875850319	27.874431444291385	22.34602353016252
76-77	22.15858492713685	27.78756338518044	27.649691675062975	22.40416001261973
78-79	22.22838711862597	27.70882374637725	27.662775988745892	22.400013146250895
80-81	22.140312309805235	27.565920370186568	27.73685177949725	22.556915540510946
82-83	22.32650349800071	27.50333702274656	27.846133127386764	22.324026351865967
84-85	22.570343861704398	27.441004003680273	27.632243923509343	22.356408211105986
86-87	22.078915151917492	27.687121191631743	27.84164904013544	22.392314616315325
88-89	22.437683467401776	27.47313190624864	27.738793533292856	22.350391093056732
90-91	22.48869918068296	27.43344156538497	27.862736747429285	22.215122506502784
92-93	22.562774381622695	27.434628540898398	27.634963459390804	22.367633618088107
94-95	22.344433495121756	27.36763476244256	27.77820817293693	22.509723569498753
96-97	22.248846356500575	27.569445929868188	27.995878039247074	22.185829674384166
98-99	23.384217092078984	29.48420989682844	28.125548702949764	19.00602430814281
100	22.640245681454623	30.237016061645328	21.85058649294383	25.272151763956224
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2755.0
1	2416.5
2	3503.0
3	5238.5
4	7077.0
5	11944.5
6	16132.0
7	18971.5
8	20737.0
9	19562.5
10	16384.5
11	13507.0
12	14573.5
13	20088.0
14	32500.0
15	49729.5
16	63696.5
17	71106.0
18	71633.0
19	66593.5
20	58661.5
21	50119.5
22	44553.5
23	44643.5
24	49316.0
25	58692.5
26	74430.5
27	91890.0
28	103160.0
29	115480.0
30	129354.5
31	140937.0
32	159593.0
33	181812.5
34	205146.0
35	226429.5
36	250534.5
37	274476.5
38	282487.0
39	288863.0
40	300677.5
41	305638.0
42	308172.0
43	317807.5
44	327185.0
45	331853.5
46	334440.5
47	337315.5
48	337028.5
49	329941.0
50	320200.0
51	308320.0
52	292496.5
53	273383.5
54	251773.5
55	229633.5
56	207808.5
57	185022.0
58	162682.0
59	143268.0
60	119318.0
61	91579.0
62	68729.0
63	50389.5
64	36316.0
65	26148.0
66	19109.5
67	14232.0
68	10741.0
69	7925.0
70	5920.5
71	4389.5
72	3174.0
73	2250.5
74	1590.0
75	1020.5
76	672.0
77	428.0
78	252.5
79	148.5
80	90.5
81	55.0
82	40.5
83	30.0
84	19.5
85	12.5
86	10.5
87	13.5
88	13.5
89	10.0
90	6.5
91	4.0
92	3.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.220868699356077E-5
2	0.001770259614066312
3	2.5638242686477626E-4
4	1.220868699356077E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.4939091471023474E-5
12-13	0.0069345342123425194
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.930876379664261E-4
26-27	0.0
28-29	1.2222438355673811E-5
30-31	1.2237547438852648E-5
32-33	6.131790198836174E-6
34-35	5.902441132833393E-4
36-37	0.02418703787023753
38-39	0.11784986263452739
40-41	0.13953280282662192
42-43	0.0304138731901363
44-45	0.0021796366842583353
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.007003059255885675
64-65	0.07215774796946853
66-67	0.17763345968440603
68-69	0.18494859092257898
70-71	0.06902828022734517
72-73	0.0021971436852201223
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	117.0
20-21	517.0
22-23	1181.0
24-25	2195.0
26-27	3594.0
28-29	7729.0
30-31	16682.0
32-33	20536.0
34-35	26652.0
36-37	35700.0
38-39	40090.0
40-41	47581.0
42-43	57717.0
44-45	66949.0
46-47	68882.0
48-49	56173.0
50-51	45840.0
52-53	41919.0
54-55	42413.0
56-57	45357.0
58-59	51636.0
60-61	51187.0
62-63	53803.0
64-65	56853.0
66-67	65321.0
68-69	61136.0
70-71	62472.0
72-73	62397.0
74-75	60690.0
76-77	55007.0
78-79	59806.0
80-81	64905.0
82-83	61033.0
84-85	67464.0
86-87	76633.0
88-89	84509.0
90-91	86405.0
92-93	93689.0
94-95	104929.0
96-97	275771.0
98-99	513150.0
100-101	5494269.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.48124873684249
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.35859275989658	65.28331764696492
2	8.972250687640255	13.724178731414405
3	2.790144765133388	6.401812673818967
4	1.1877142237201412	3.6335146789050365
5	0.5981153282886718	2.287230359808205
6	0.3454350981327708	1.585158459763682
7	0.20020153827077272	1.0718164552189822
8	0.13877711807725077	0.8491077829318701
9	0.09672007547950758	0.6657544935534958
>10	0.3039695696003529	3.4563513105062635
>50	0.0049498693382579026	0.2585575619652127
>100	0.0025919103831889057	0.3613625163786556
>500	2.1482243037496726E-4	0.12448377813061623
>1k	3.2223360854530025E-4	0.29735355063964153
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2208686993560772E-5	0.0	0.0	0.0
3	0.0	1.2208686993560772E-5	0.0	0.0	0.0
4	0.0	3.6626060980682314E-5	0.0	0.0	0.0
5	0.0	3.6626060980682314E-5	0.0	0.0	0.0
6	0.0	3.6626060980682314E-5	0.0	0.0	0.0
7	0.0	3.6626060980682314E-5	0.0	0.0	0.0
8	0.0	4.883474797424309E-5	0.0	0.0	0.0
9	0.0	6.104343496780386E-5	0.0	0.0	0.0
10-11	0.0	6.104343496780386E-5	0.0	0.0	0.0
12-13	0.0	6.104343496780386E-5	0.0	0.0	0.0
14-15	0.0	9.15651524517058E-5	0.0	1.831303049034116E-5	0.0
16-17	0.0	9.766949594848618E-5	0.0	6.104343496780386E-5	0.0
18-19	0.0	1.0377383944526656E-4	0.0	1.1598252643882733E-4	0.0
20-21	0.0	1.0987818294204695E-4	0.0	1.770259614066312E-4	0.0
22-23	0.0	1.342955569291685E-4	0.0	4.2119970127784664E-4	0.0
24-25	0.0	1.5260858741950964E-4	0.0	0.0011048861729172498	0.0
26-27	0.0	1.5871293091629003E-4	0.0	0.00284462406949966	0.0
28-29	0.0	1.5871293091629003E-4	0.0	0.009205349993144821	0.0
30-31	0.0	1.831303049034116E-4	0.0	0.011756965574799024	0.0
32-33	0.0	1.9533899189697236E-4	0.0	0.01659160562424909	0.0
34-35	0.0	2.136520223873135E-4	0.0	0.022482297098642162	0.0
36-37	0.0	2.3196505287765467E-4	0.0	0.03167543840479342	0.0
38-39	0.0	2.3196505287765467E-4	0.0	0.054591143891706995	0.0
40-41	0.0	2.563824268647762E-4	0.0	0.07886811797840258	0.0
42-43	0.0	2.68591113858337E-4	0.0	0.09406182894188897	0.0
44-45	0.0	2.991128313422389E-4	0.0	0.11242369418020437	0.0
46-47	0.0	3.1742586183258005E-4	0.0	0.13070620295306162	0.0
48-49	0.0	3.1742586183258005E-4	0.0	0.14908027687837058	0.0
50-51	0.0	3.7846929680038395E-4	0.0	0.16565967381562613	0.0
52-53	0.0	3.7846929680038395E-4	0.0	0.2197014267926229	0.0
54-55	0.0	3.8457364029716433E-4	0.0	0.28771602203374996	0.0
56-57	0.0	3.906779837939447E-4	0.0	0.34439485140135584	0.0
58-59	0.0	3.906779837939447E-4	0.0	0.365930975257997	0.0
60-61	0.0	3.906779837939447E-4	0.0	0.38341991937627284	0.0
62-63	0.0	4.2119970127784664E-4	0.0	0.39567744111780784	0.0
64-65	0.0	5.249735407231132E-4	0.0	0.4005731246022257	0.0
66-67	0.0	5.55495258207015E-4	0.0	0.40539555596468224	0.0
68-69	0.0	5.799126321941366E-4	0.0	0.410517100158481	0.0
70-71	0.0	5.86016975690917E-4	0.0	0.414436088683414	0.0
72-73	0.0	5.86016975690917E-4	0.0	0.41755540821026876	0.0
74-75	0.0	5.921213191876975E-4	0.0	0.42014975419640044	0.0
76-77	0.0	6.226430366715993E-4	0.0	0.42259759593860935	0.0
78-79	0.0	6.531647541555014E-4	0.0	0.4243129164612046	0.0
80-81	0.0	6.592690976522817E-4	0.0	0.42481347262794067	0.0
82-83	0.0	6.592690976522817E-4	0.0	0.4253201331381734	0.0
84-85	0.0	6.653734411490621E-4	0.0	0.42552157647356714	0.0
86-87	0.0	6.836864716394033E-4	0.0	0.4256558720304964	0.0
88	0.0	7.203125326200856E-4	0.0	0.4256680807174899	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13835	0.0	52.073864	1
GTATCAA	24680	0.0	38.328873	1
ATCAACG	31470	0.0	29.607342	3
TCAACGC	31990	0.0	29.166075	4
TATCAAC	32470	0.0	28.974195	2
CAACGCA	32220	0.0	28.904446	5
AACGCAG	32890	0.0	28.461477	6
ACGCAGA	36760	0.0	25.465128	7
CGCAGAG	37220	0.0	25.173532	8
CGCTACG	225	2.592289E-6	22.86243	94
TACATGG	26990	0.0	22.802197	2
GCAGAGT	41905	0.0	22.276955	9
ACATGGG	27075	0.0	22.221684	3
GTACATG	28550	0.0	22.053713	1
GAGTACT	28975	0.0	20.653566	12-13
CATGGGG	18785	0.0	20.64175	4
GTGGTAT	5975	0.0	19.663836	1
CAGAGTA	41880	0.0	19.654266	10-11
AGAGTAC	39850	0.0	19.283844	10-11
TGGTATC	5695	0.0	19.194798	2
>>END_MODULE