FastQCFastQC Report
Fri 27 May 2016
SRR2138915_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138915_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7056534
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114400.054.731911
GTATCAA210500.039.4033241
ATCAACG260000.031.226343
TCAACGC265100.030.6751884
CAACGCA266200.030.4990545
TATCAAC271250.030.223062
AACGCAG276450.029.4195336
CTTATAC69900.026.2646261
ACGCAGA310150.026.1663767
CGCAGAG311850.026.0518388
GTACATG221450.023.3673021
TACATGG213500.023.2318042
GCAGAGT353600.022.9262949
TATACAC96350.022.05513
ACATGGG222100.021.9566253
GAGTACT251350.021.19831512-13
TGGTATC48800.021.0102122
GTGGTAT53800.020.7678851
CAGAGTA345800.020.63021910-11
CATGGGG168250.019.9216524
Produced by FastQC (version 0.11.5)