##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138915_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7056534
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6953903998762	33.0	33.0	33.0	33.0	33.0
2	32.69886051707538	33.0	33.0	33.0	33.0	33.0
3	32.71064732345936	33.0	33.0	33.0	33.0	33.0
4	36.6346499570469	37.0	37.0	37.0	37.0	37.0
5	36.64743796317002	37.0	37.0	37.0	37.0	37.0
6	36.5877043885851	37.0	37.0	37.0	37.0	37.0
7	36.56211703932837	37.0	37.0	37.0	37.0	37.0
8	36.560486493794265	37.0	37.0	37.0	37.0	37.0
9	36.60282158351394	37.0	37.0	37.0	37.0	37.0
10-11	36.58188857305868	37.0	37.0	37.0	37.0	37.0
12-13	36.57032163382193	37.0	37.0	37.0	37.0	37.0
14-15	38.66803709866629	40.0	37.0	40.0	37.0	40.0
16-17	38.60427732651752	40.0	37.0	40.0	37.0	40.0
18-19	38.63168887161884	40.0	37.0	40.0	37.0	40.0
20-21	38.64760089057366	40.0	37.0	40.0	37.0	40.0
22-23	38.6410805628709	40.0	37.0	40.0	37.0	40.0
24-25	38.64112106079946	40.0	37.0	40.0	37.0	40.0
26-27	38.55130770652798	40.0	37.0	40.0	37.0	40.0
28-29	38.42459002173632	40.0	37.0	40.0	37.0	40.0
30-31	38.28693192195614	40.0	37.0	40.0	37.0	40.0
32-33	38.166814711809415	40.0	37.0	40.0	37.0	40.0
34-35	38.13060516002696	40.0	37.0	40.0	37.0	40.0
36-37	38.11106319955095	40.0	37.0	40.0	37.0	40.0
38-39	38.05185694428251	40.0	37.0	40.0	37.0	40.0
40-41	38.03091108535567	40.0	37.0	40.0	37.0	40.0
42-43	37.96740809632472	40.0	37.0	40.0	33.0	40.0
44-45	37.8595191475836	40.0	37.0	40.0	33.0	40.0
46-47	37.74911185842043	40.0	37.0	40.0	33.0	40.0
48-49	37.68161816474786	40.0	37.0	40.0	33.0	40.0
50-51	37.634975258431794	40.0	37.0	40.0	33.0	40.0
52-53	37.574202237338724	40.0	37.0	40.0	33.0	40.0
54-55	37.454983184017976	40.0	37.0	40.0	33.0	40.0
56-57	37.27012158711368	38.5	37.0	40.0	33.0	40.0
58-59	37.079467900565064	37.0	37.0	40.0	33.0	40.0
60-61	36.89925118469844	37.0	37.0	40.0	33.0	40.0
62-63	36.75540817823253	37.0	37.0	40.0	33.0	40.0
64-65	36.59060326277595	37.0	37.0	40.0	33.0	40.0
66-67	36.386765922552854	37.0	37.0	40.0	33.0	40.0
68-69	36.18461735379252	37.0	37.0	40.0	33.0	40.0
70-71	35.96056728260521	37.0	37.0	37.0	33.0	40.0
72-73	35.67148266858257	37.0	33.0	37.0	33.0	40.0
74-75	35.31407854634011	37.0	33.0	37.0	33.0	40.0
76-77	33.21833172286885	35.0	33.0	37.0	27.0	37.0
78-79	34.891195297421305	37.0	33.0	37.0	33.0	37.0
80-81	35.11360355931323	37.0	33.0	37.0	33.0	37.0
82-83	34.923334092678544	37.0	33.0	37.0	33.0	37.0
84-85	34.776747338573884	37.0	33.0	37.0	33.0	37.0
86-87	34.67544438440571	37.0	33.0	37.0	33.0	37.0
88-89	34.56924024164722	37.0	33.0	37.0	33.0	37.0
90-91	34.49618622125656	37.0	33.0	37.0	33.0	37.0
92-93	34.45097261109023	37.0	33.0	37.0	33.0	37.0
94-95	34.407794059766445	37.0	33.0	37.0	33.0	37.0
96-97	34.42450054257481	37.0	33.0	37.0	33.0	37.0
98-99	34.477028304788476	37.0	33.0	37.0	33.0	37.0
100	34.46068456392064	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	2.0
18	2.0
19	8.0
20	43.0
21	187.0
22	851.0
23	2484.0
24	5793.0
25	11608.0
26	21031.0
27	33823.0
28	51196.0
29	72424.0
30	98443.0
31	133359.0
32	178848.0
33	242046.0
34	339011.0
35	516273.0
36	951578.0
37	2227257.0
38	2122351.0
39	47915.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.66102532220851	20.38719297422686	14.518319406994909	23.43346229656972
2	14.62189215900514	20.67365263945232	42.15896262498891	22.545492576553627
3	17.96016762367745	26.38447472911935	30.77430082047513	24.88105682672807
4	12.12351731367231	18.012499906115366	38.89512030908096	30.968862471131363
5	13.059090065900634	37.93901339368781	35.59848724579054	13.403409294621028
6	29.661063065805394	37.952853341314594	18.630123513895064	13.755960078984952
7	26.052265885773384	33.0947034337254	22.94608089467152	17.906949785829703
8	24.590826034424264	36.291003486981005	21.165943507109862	17.952226971484865
9	25.64508298266543	16.53174207054058	21.166538700160732	36.65663624663326
10-11	24.11409032085449	26.574498634740124	28.88759662678494	20.423814417620438
12-13	25.04265508430606	24.285407759079945	28.955797583821013	21.71613957279298
14-15	22.161077945631664	25.632031249335725	26.92461341502783	25.282277390004783
16-17	21.489523043465812	28.664284760762154	27.63846245196296	22.20772974380907
18-19	21.336494658709217	27.406549731071937	29.886414491873776	21.37054111834507
20-21	22.218143152803687	26.717028411864042	29.811270645948866	21.253557789383404
22-23	21.94000915989141	26.744119220033046	29.69506263711757	21.620808982957968
24-25	21.642221323047302	26.849294621372348	30.007602823995157	21.500881231585193
26-27	21.710219078031344	27.088044615471112	29.788421499136152	21.413314807361395
28-29	21.47811440121018	27.037574449992547	29.85236333993913	21.631947808858143
30-31	21.99077205100017	26.894423745946895	29.756233920973752	21.358570282079185
32-33	21.884212826391057	27.035406904328212	29.788732098927923	21.29164817035281
34-35	21.517498999459896	27.14185561068488	29.987791238367006	21.352854151488216
36-37	21.773606301353198	27.04276966667057	29.671600819109667	21.512023212866566
38-39	21.61224541831026	27.160422382647692	29.7276469032788	21.499685295763253
40-41	21.776030726346065	27.07033783063561	29.641856371915665	21.511775071102658
42-43	21.87435786760151	27.095612249226704	29.39776380734555	21.632266075826234
44-45	21.719656501101518	27.51089488129972	29.047570775575753	21.72187784202301
46-47	22.06781883057241	27.210634266477523	28.458118101239236	22.263428801710823
48-49	22.056253395848167	27.35306662654101	28.496911241896804	22.09376873571402
50-51	21.939229256636526	27.61772330835599	28.34488724773839	22.098160187269094
52-53	22.04276950100724	27.7508096584118	28.277006468413727	21.929414372167226
54-55	22.037918432776127	27.66668848093328	28.166247538761517	22.129145547529074
56-57	22.234006536160912	27.86020343097061	27.859883191556644	22.045906841311837
58-59	22.019873446096376	27.948447675081546	28.1525796663198	21.87909921250228
60-61	22.14271349706503	28.125579547358516	27.853671444036593	21.878035511539863
62-63	21.93663982334522	28.163596553619907	27.819719047274937	22.08004457575993
64-65	21.940233500058785	28.032305284187913	27.88674825255652	22.140712963196783
66-67	22.10502455423837	27.960689179816477	27.762199648016622	22.172086617928528
68-69	22.065820531957968	27.85485676992684	27.868414942303872	22.210907755811327
70-71	22.098826068197713	27.52517087504649	27.860343803547188	22.51565925320861
72-73	22.20454320487142	27.56258790502618	27.85541652833145	22.37745236177095
74-75	22.094793635963185	27.722819082242	27.88216273473882	22.300224547056
76-77	22.129711859181818	27.66123136977006	27.738239408526923	22.4708173625212
78-79	22.195302131676744	27.6310616678063	27.64809956338372	22.525536637133236
80-81	22.23140237131567	27.62296263467344	27.72379752276204	22.42183747124885
82-83	22.445029102336743	27.389307771199416	27.825431400506346	22.340231725957494
84-85	22.56650143822246	27.420023661012195	27.605292127128315	22.40818277363703
86-87	22.21537869164601	27.596614911336044	27.733847328264776	22.45415906875317
88-89	22.524185051346045	27.436009805841387	27.661081384222463	22.378723758590105
90-91	22.559484103378384	27.455149000234556	27.687585429606003	22.29778146678105
92-93	22.55403943303735	27.315721990087244	27.62693647178123	22.503302105094175
94-95	22.39721367842474	27.429859978279662	27.71045627455339	22.462470068742206
96-97	22.55894413871232	27.460170932938823	27.852554249761567	22.128330678587293
98-99	22.592467531101708	28.250223295274857	26.958077955404047	22.199231218219392
100	20.79713440467053	27.884273976659436	28.053250575657394	23.26534104301264
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1943.0
1	1808.0
2	2840.5
3	4167.0
4	5468.0
5	9222.0
6	12716.0
7	15484.0
8	17345.0
9	16628.0
10	13982.0
11	11599.5
12	12461.0
13	16809.0
14	27331.5
15	42123.0
16	53768.5
17	59730.0
18	60047.5
19	55486.5
20	48346.0
21	40632.0
22	34899.5
23	33535.5
24	35939.0
25	41829.0
26	51800.0
27	62712.0
28	72863.0
29	84681.5
30	96041.5
31	106725.0
32	121773.5
33	139610.0
34	158014.0
35	174317.5
36	192677.5
37	213067.0
38	224387.0
39	230199.0
40	241058.0
41	251467.5
42	257518.5
43	263896.0
44	271205.0
45	281065.0
46	288704.5
47	294006.5
48	297467.0
49	298388.5
50	294113.0
51	282071.0
52	266167.5
53	249966.5
54	230495.5
55	205093.0
56	180592.0
57	155633.5
58	133262.5
59	115980.5
60	96329.5
61	76080.0
62	58293.5
63	43275.0
64	32037.0
65	23533.5
66	17220.0
67	13026.5
68	9816.0
69	7217.5
70	5248.0
71	3647.5
72	2498.0
73	1731.0
74	1276.0
75	876.0
76	567.5
77	358.0
78	228.0
79	153.5
80	105.5
81	78.0
82	56.5
83	38.0
84	25.5
85	16.5
86	12.0
87	7.0
88	3.5
89	2.0
90	2.5
91	3.0
92	2.5
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.4171263115858295E-5
2	0.001530496416512696
3	1.8422642050615784E-4
4	1.4171263115858295E-5
5	1.4171263115858295E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	2.834252623171659E-5
12-13	0.007326543030898739
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.7641401716743674E-4
26-27	7.089742815325528E-6
28-29	1.418669118126903E-5
30-31	1.4200556590815576E-5
32-33	1.4221094647428844E-5
34-35	6.481477788331745E-4
36-37	0.024952001655853658
38-39	0.1212081326193232
40-41	0.14228987283138486
42-43	0.031140360729938695
44-45	0.0020910557081841316
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.006831937961761749
64-65	0.07116004675186101
66-67	0.17466903640222703
68-69	0.18247228713300562
70-71	0.06916317490759388
72-73	0.0020522920866300905
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	99.0
20-21	453.0
22-23	988.0
24-25	1878.0
26-27	3053.0
28-29	5721.0
30-31	9864.0
32-33	11124.0
34-35	14220.0
36-37	15543.0
38-39	17227.0
40-41	24103.0
42-43	32492.0
44-45	38923.0
46-47	39520.0
48-49	31613.0
50-51	25488.0
52-53	29487.0
54-55	32702.0
56-57	34646.0
58-59	29415.0
60-61	46399.0
62-63	41088.0
64-65	51371.0
66-67	65845.0
68-69	53385.0
70-71	52824.0
72-73	52107.0
74-75	50584.0
76-77	45679.0
78-79	51322.0
80-81	57805.0
82-83	53804.0
84-85	62092.0
86-87	65776.0
88-89	71686.0
90-91	73943.0
92-93	79775.0
94-95	90943.0
96-97	255920.0
98-99	40811.0
100-101	5294816.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.45230902947637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.85474377087235	65.72195837158641
2	9.647816842404529	14.94491383075405
3	2.7927807421148425	6.489219512695474
4	1.2186888605227266	3.775610649439466
5	0.5719171876025266	2.2148153376729955
6	0.31484725506859373	1.4631388137993055
7	0.181516303546394	0.9841199785314484
8	0.11194835083339898	0.6936526611270926
9	0.07285668319611108	0.5078626507591069
>10	0.2280219830137952	2.5576246370570614
>50	0.003224977766889473	0.17094907942292856
>100	0.0011587381529306014	0.1724727482441467
>500	4.415122206821327E-4	0.2572404686648557
>1k	3.679268418278428E-5	0.046421260245734154
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4171263115858295E-5
2	0.0	0.0	0.0	0.0	1.4171263115858295E-5
3	0.0	0.0	0.0	0.0	1.4171263115858295E-5
4	0.0	0.0	0.0	0.0	1.4171263115858295E-5
5	0.0	0.0	0.0	0.0	1.4171263115858295E-5
6	0.0	0.0	0.0	0.0	2.834252623171659E-5
7	0.0	0.0	0.0	0.0	4.2513789347574884E-5
8	0.0	0.0	0.0	0.0	4.2513789347574884E-5
9	0.0	0.0	0.0	1.4171263115858295E-5	4.2513789347574884E-5
10-11	0.0	0.0	0.0	1.4171263115858295E-5	4.2513789347574884E-5
12-13	0.0	0.0	0.0	1.4171263115858295E-5	4.2513789347574884E-5
14-15	0.0	0.0	0.0	4.2513789347574884E-5	4.959942090550403E-5
16-17	0.0	0.0	0.0	8.502757869514977E-5	5.668505246343318E-5
18-19	0.0	0.0	0.0	9.211321025307892E-5	7.085631557929147E-5
20-21	0.0	0.0	0.0	1.1337010492686636E-4	7.085631557929147E-5
22-23	0.0	0.0	0.0	3.4011031478059907E-4	8.502757869514977E-5
24-25	0.0	0.0	0.0	9.56560260320435E-4	9.919884181100806E-5
26-27	0.0	0.0	0.0	0.0020831756780311694	9.919884181100806E-5
28-29	0.0	0.0	0.0	0.006695921822243045	9.919884181100806E-5
30-31	0.0	0.0	0.0	0.007383228083362172	9.919884181100806E-5
32-33	0.0	7.085631557929147E-6	0.0	0.008502757869514977	1.1337010492686636E-4
34-35	0.0	2.1256894673787442E-5	0.0	0.009735657760594648	1.4171263115858295E-4
36-37	0.0	3.5428157789645736E-5	0.0	0.01115278407218048	1.487982627165121E-4
38-39	0.0	4.2513789347574884E-5	0.0	0.01421377690520587	1.7005515739029953E-4
40-41	0.0	4.959942090550403E-5	0.0	0.017303112264462978	1.7005515739029953E-4
42-43	0.0	7.085631557929147E-5	0.0	0.020555417149552457	1.771407889482287E-4
44-45	0.0	7.085631557929147E-5	0.0	0.02403446224449567	1.8422642050615784E-4
46-47	0.0	9.919884181100806E-5	0.0	0.028413382547295883	1.8422642050615784E-4
48-49	1.4171263115858295E-5	1.3462699960065382E-4	0.0	0.03373469184730067	1.9839768362201612E-4
50-51	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.038234067886585676	1.9839768362201612E-4
52-53	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.052291960897517115	2.267402098537327E-4
54-55	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.07104054199979763	2.5508273608544933E-4
56-57	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.08679898658463206	2.5508273608544933E-4
58-59	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.17634011258218268	2.7633963075923674E-4
60-61	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.2588310351795938	3.117677885488825E-4
62-63	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.26266436185243347	3.259390516647408E-4
64-65	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.2657466115801327	3.259390516647408E-4
66-67	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.27028850140876526	3.471959463385282E-4
68-69	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.2754822693407273	3.542815778964574E-4
70-71	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.2797832476963903	3.6136720945438653E-4
72-73	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.2829080112134371	3.9679536724403225E-4
74-75	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.2856997500472611	3.9679536724403225E-4
76-77	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.28832851935525283	3.9679536724403225E-4
78-79	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.29004324219227173	3.9679536724403225E-4
80-81	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.2908084904005281	4.1096663035989056E-4
82-83	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.29130448460958314	4.2513789347574886E-4
84-85	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.2914745397669734	4.2513789347574886E-4
86-87	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.2916020811350162	4.2513789347574886E-4
88	1.4171263115858295E-5	1.4171263115858295E-4	0.0	0.29163042366124786	4.2513789347574886E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11440	0.0	54.73191	1
GTATCAA	21050	0.0	39.403324	1
ATCAACG	26000	0.0	31.22634	3
TCAACGC	26510	0.0	30.675188	4
CAACGCA	26620	0.0	30.499054	5
TATCAAC	27125	0.0	30.22306	2
AACGCAG	27645	0.0	29.419533	6
CTTATAC	6990	0.0	26.264626	1
ACGCAGA	31015	0.0	26.166376	7
CGCAGAG	31185	0.0	26.051838	8
GTACATG	22145	0.0	23.367302	1
TACATGG	21350	0.0	23.231804	2
GCAGAGT	35360	0.0	22.926294	9
TATACAC	9635	0.0	22.0551	3
ACATGGG	22210	0.0	21.956625	3
GAGTACT	25135	0.0	21.198315	12-13
TGGTATC	4880	0.0	21.010212	2
GTGGTAT	5380	0.0	20.767885	1
CAGAGTA	34580	0.0	20.630219	10-11
CATGGGG	16825	0.0	19.921652	4
>>END_MODULE