FastQCFastQC Report
Fri 27 May 2016
SRR2138914_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138914_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7684965
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT46000.034.84777594
TATCTCG53550.032.16851894
GGTATCA234300.032.158331
GTATCAA338200.027.1595521
ATCAACG342600.025.768153
TCAACGC352700.025.0178974
CAACGCA358400.024.5843565
AACGCAG360450.024.4820636
ACGCAGA364350.024.2439167
CGCTATC81400.023.02397294
GTACATG299150.022.624641
TATCAAC401700.022.6061552
ACATGGG285700.022.4700533
TACATGG295900.022.4022772
CGCAGAG416200.021.1817598
GAGTACT239500.021.12219212-13
GCAGAGT463050.018.982099
CAGAGTA411950.018.97888610-11
GTACTTT265800.018.92694714-15
AGAGTAC370950.018.73407210-11
Produced by FastQC (version 0.11.5)