##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138914_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7684965
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.781669272403974	33.0	33.0	33.0	33.0	33.0
2	32.82877007247268	33.0	33.0	33.0	33.0	33.0
3	33.512260628382826	33.0	33.0	33.0	33.0	37.0
4	36.6496503237165	37.0	37.0	37.0	37.0	37.0
5	36.65156353997708	37.0	37.0	37.0	37.0	37.0
6	36.5977671986795	37.0	37.0	37.0	37.0	37.0
7	36.585026477023646	37.0	37.0	37.0	37.0	37.0
8	36.575324676169636	37.0	37.0	37.0	37.0	37.0
9	36.61616142689004	37.0	37.0	37.0	37.0	37.0
10-11	36.61323877206988	37.0	37.0	37.0	37.0	37.0
12-13	36.84654471425699	37.0	37.0	37.0	37.0	38.5
14-15	38.710548258840475	40.0	37.0	40.0	37.0	40.0
16-17	38.64826385025826	40.0	37.0	40.0	37.0	40.0
18-19	38.6772570206891	40.0	37.0	40.0	37.0	40.0
20-21	38.6937589652187	40.0	37.0	40.0	37.0	40.0
22-23	38.6924866123084	40.0	37.0	40.0	37.0	40.0
24-25	38.67950258179359	40.0	37.0	40.0	37.0	40.0
26-27	38.59238763557873	40.0	37.0	40.0	37.0	40.0
28-29	38.483373986051234	40.0	37.0	40.0	37.0	40.0
30-31	38.35575810916294	40.0	37.0	40.0	37.0	40.0
32-33	38.251657283432706	40.0	37.0	40.0	37.0	40.0
34-35	38.23555086249554	40.0	37.0	40.0	37.0	40.0
36-37	38.21044161963526	40.0	37.0	40.0	37.0	40.0
38-39	38.16710837701224	40.0	37.0	40.0	37.0	40.0
40-41	38.13548872481363	40.0	37.0	40.0	37.0	40.0
42-43	38.07356157491117	40.0	37.0	40.0	37.0	40.0
44-45	37.97273599484397	40.0	37.0	40.0	33.0	40.0
46-47	37.8704809215112	40.0	37.0	40.0	33.0	40.0
48-49	37.80565382719586	40.0	37.0	40.0	33.0	40.0
50-51	37.77068849853693	40.0	37.0	40.0	33.0	40.0
52-53	37.70697739964913	40.0	37.0	40.0	33.0	40.0
54-55	37.59094254312498	40.0	37.0	40.0	33.0	40.0
56-57	37.41614960371169	40.0	37.0	40.0	33.0	40.0
58-59	37.237033742962026	38.5	37.0	40.0	33.0	40.0
60-61	37.065799920329184	37.0	37.0	40.0	33.0	40.0
62-63	36.92172026738522	37.0	37.0	40.0	33.0	40.0
64-65	36.74410540300596	37.0	37.0	40.0	33.0	40.0
66-67	36.532678039830074	37.0	37.0	40.0	33.0	40.0
68-69	36.322459305742925	37.0	37.0	40.0	33.0	40.0
70-71	36.084212106186435	37.0	37.0	38.5	33.0	40.0
72-73	35.78154383459088	37.0	35.0	37.0	33.0	40.0
74-75	35.16964182988805	37.0	33.0	37.0	30.0	40.0
76-77	33.614672470685846	35.0	33.0	37.0	27.0	37.0
78-79	35.0446882213316	37.0	33.0	37.0	33.0	37.0
80-81	35.18302178229982	37.0	33.0	37.0	33.0	37.0
82-83	34.99304002963337	37.0	33.0	37.0	33.0	37.0
84-85	34.85063820833625	37.0	33.0	37.0	33.0	37.0
86-87	34.750243640008904	37.0	33.0	37.0	33.0	37.0
88-89	34.64564358976196	37.0	33.0	37.0	33.0	37.0
90-91	34.58526529052081	37.0	33.0	37.0	33.0	37.0
92-93	34.548198340177876	37.0	33.0	37.0	33.0	37.0
94-95	34.50468068111867	37.0	33.0	37.0	33.0	37.0
96-97	34.51649022086396	37.0	33.0	37.0	33.0	37.0
98-99	34.53445636888728	37.0	33.0	37.0	33.0	37.0
100	34.45964467968954	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	10.0
20	58.0
21	236.0
22	792.0
23	2399.0
24	5894.0
25	11933.0
26	21380.0
27	34625.0
28	52107.0
29	74678.0
30	101705.0
31	137888.0
32	183065.0
33	247983.0
34	347991.0
35	522720.0
36	948585.0
37	2308498.0
38	2603903.0
39	78510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.45550724169987	0.016994285964125142	24.427789639367198	29.099708832968812
2	15.31551144224433	23.411850527843335	39.06627528219136	22.206362747720974
3	17.245932314546856	24.351005080052467	32.61856925735462	25.784493348046055
4	13.085903850688174	22.751414320223233	37.427540324196706	26.735141504891892
5	17.49106436933211	36.4508539012024	32.03118453124829	14.026897198217195
6	28.05379439643439	36.867329502129095	20.25853341668224	14.820342684754282
7	26.607824498875406	33.00402799492255	21.97613131614783	18.412016190054217
8	23.925710507649807	32.34563393473723	22.097595004686426	21.63106055292654
9	23.857832814050106	20.749043064940416	23.590882545525332	31.802241575484143
10-11	24.87143053540318	26.03451061233633	28.287429291918187	20.8066295603423
12-13	25.195398038898137	24.184806142073015	28.53161748252799	22.088178336500857
14-15	22.383706366912538	26.60233325721067	26.92405886038518	24.08990151549161
16-17	21.116563315512824	28.945577501003584	28.00714121664835	21.930717966835243
18-19	21.24081892370362	28.000693562039647	29.868691659623693	20.88979585463304
20-21	22.020354781383812	27.06579870578889	29.91423699241314	20.999609520414158
22-23	21.86469357295155	26.800870726006565	29.777165915321525	21.55726978572036
24-25	21.59949916091747	27.051475250889872	29.98456978687063	21.364455801322027
26-27	21.425665124142178	27.235659797157812	29.949282941016346	21.38939213768366
28-29	21.35769752128996	27.156530908513677	30.066357503985085	21.419414066211274
30-31	21.55510467174871	27.21892750002789	29.940743273757832	21.28522455446557
32-33	21.3191780620521	27.586286658945415	29.71057055065399	21.383964728348495
34-35	21.57043803987667	27.291761305760502	29.637496772420814	21.500303881942013
36-37	21.496742554497086	27.332336958074787	29.742090513188003	21.428829974240124
38-39	21.506365793660425	27.471639950312348	29.65549942769271	21.366494828334513
40-41	21.683807847140756	27.280086966161626	29.574464775891705	21.46164041080591
42-43	21.47444437017635	27.510079679331916	29.344527499209665	21.670948451282072
44-45	21.60705337332848	27.758603686680623	28.948342277416682	21.686000662574216
46-47	21.825688892632996	27.54394507392275	28.66377658902321	21.966589444421043
48-49	21.73325502205051	27.74553812412791	28.648437791757626	21.872769062063956
50-51	21.783182492745798	27.969973792239212	28.362366081785375	21.884477633229615
52-53	21.886046545873462	27.72859164812555	28.308588066040496	22.076773739960487
54-55	21.87401245912104	27.57441229868574	28.47085757003642	22.080717672156805
56-57	21.83113609106921	27.671315382684252	28.305414257933265	22.192134268313275
58-59	21.834172745164153	27.279354792999438	28.496523177702176	22.389949284134232
60-61	21.788813683645973	27.502871788561055	28.722356720815778	21.98595780697719
62-63	21.534206484685022	27.763801533005633	28.611626382831528	22.090365599477813
64-65	21.56167618893129	27.774672331356427	28.43484026962681	22.22881121008547
66-67	21.538553968507713	28.13394182787123	28.13194625337611	22.195557950244947
68-69	21.561027800282538	28.299757433790212	27.929821486853655	22.209393279073588
70-71	21.71984384333441	27.627157630967382	27.898045310160462	22.754953215537746
72-73	21.770226791884575	27.53010706360065	27.878845979104362	22.820820165410417
74-75	21.849360990243003	27.64656102625014	27.89867673775348	22.605401245753377
76-77	22.216281854332735	27.338837544974776	27.52841205200629	22.916468548686197
78-79	22.140652668333015	27.483958540642746	27.38349935168693	22.99188943933731
80-81	22.13724393922288	27.780104758325198	27.247697768853097	22.83495353359883
82-83	22.244885973557484	27.4495653218301	27.383209223973786	22.92233948063863
84-85	22.273434172534134	27.7401093646349	27.384009714111574	22.602446748719395
86-87	22.493284314167838	27.739814327840236	27.38556763655292	22.381333721439006
88-89	22.464699804038048	27.59695636519653	27.501320813372836	22.437023017392587
90-91	22.33678174170003	27.431371423484062	27.542341321742807	22.6895055130731
92-93	22.328907486696657	27.324603111265382	27.661813908036365	22.6846754940016
94-95	22.034317131682606	27.348532015946383	27.99351808125311	22.6236327711179
96-97	22.0924893790356	27.51826347492536	28.13343973397063	22.255807412068414
98-99	23.209068826090906	29.57606777670008	27.815293507603894	19.39956988960512
100	22.33253423844675	29.93161804846787	22.101041988084784	25.634805725000593
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2273.0
1	1956.5
2	2482.5
3	3550.5
4	4752.5
5	7771.5
6	10434.5
7	12670.5
8	14703.5
9	15087.5
10	13963.0
11	12432.5
12	13014.0
13	16290.5
14	24870.0
15	38257.0
16	50260.0
17	57154.5
18	57980.0
19	53688.5
20	46769.5
21	39293.0
22	33669.5
23	32252.5
24	35203.5
25	42299.5
26	52485.5
27	64071.0
28	76603.5
29	91649.0
30	107884.5
31	124642.5
32	144003.5
33	164341.5
34	183825.5
35	202738.5
36	223758.5
37	243502.5
38	257742.0
39	269974.0
40	282683.5
41	293891.5
42	301456.5
43	310462.0
44	320956.5
45	333597.5
46	342886.5
47	341803.0
48	336819.5
49	327255.5
50	312978.0
51	296310.0
52	275763.0
53	252615.0
54	229316.0
55	206623.5
56	184518.5
57	161446.0
58	139678.5
59	119954.0
60	99666.0
61	80656.5
62	63374.0
63	48410.0
64	36182.5
65	26289.5
66	19549.5
67	14790.0
68	11134.5
69	8195.0
70	5885.0
71	4207.0
72	2965.5
73	2047.5
74	1360.5
75	923.0
76	659.5
77	453.5
78	300.0
79	208.0
80	151.5
81	113.0
82	84.5
83	64.0
84	46.5
85	31.0
86	21.5
87	19.0
88	15.0
89	11.5
90	8.5
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.7736020918768007E-4
2	0.0014183538897054183
3	6.506210503235863E-5
4	1.3012421006471727E-5
5	1.3012421006471727E-5
6	1.3012421006471727E-5
7	0.0
8	2.6024842012943453E-5
9	1.6916147308413247E-4
10-11	0.0014508849422215976
12-13	0.005966195031467287
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.857423288255905E-5
24-25	1.8878426190235947E-4
26-27	0.0
28-29	1.3032739283699796E-5
30-31	1.3046824204024673E-5
32-33	7.841042722770722E-5
34-35	0.0023639432774102496
36-37	0.03674127301188999
38-39	0.12714280634706582
40-41	0.1305631446939463
42-43	0.026045370224542175
44-45	0.0017516791549578839
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.3770818465152772E-5
60-61	5.190890499341518E-4
62-63	0.013796721158027547
64-65	0.08529324838078126
66-67	0.18098606537268672
68-69	0.1713517656212725
70-71	0.058980393509139445
72-73	0.001869451424695888
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	377.0
20-21	1559.0
22-23	1657.0
24-25	2573.0
26-27	4404.0
28-29	6863.0
30-31	11946.0
32-33	15057.0
34-35	20798.0
36-37	21976.0
38-39	24620.0
40-41	35255.0
42-43	45484.0
44-45	58485.0
46-47	57113.0
48-49	48565.0
50-51	24866.0
52-53	12677.0
54-55	12047.0
56-57	12470.0
58-59	30886.0
60-61	44466.0
62-63	46374.0
64-65	50064.0
66-67	60439.0
68-69	58754.0
70-71	60770.0
72-73	57700.0
74-75	55775.0
76-77	54374.0
78-79	57492.0
80-81	59454.0
82-83	60110.0
84-85	68935.0
86-87	74764.0
88-89	80924.0
90-91	82958.0
92-93	90753.0
94-95	117030.0
96-97	392204.0
98-99	1448847.0
100-101	4213100.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.53367396953743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.12563044414917	73.61457681881498
2	8.383563716016974	14.006197563132108
3	2.0169024558563216	5.054378165275839
4	0.721406987951626	2.410471045235886
5	0.30198366676410454	1.261290258179907
6	0.15901433673600124	0.7969831056832419
7	0.08732667910222901	0.5106302838677609
8	0.05484660760598484	0.3665230910474791
9	0.034765523553508614	0.2613682718909156
>10	0.11099590669443968	1.4212831275063242
>50	0.0027292705818387566	0.14970182053518902
>100	8.085897138663554E-4	0.13214826951041214
>500	2.5815273914620772E-5	0.014448179319942179
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.204968402588691E-5	0.0	0.0	0.0
3	0.0	5.204968402588691E-5	0.0	0.0	0.0
4	0.0	5.204968402588691E-5	0.0	0.0	0.0
5	0.0	6.506210503235863E-5	0.0	0.0	0.0
6	0.0	6.506210503235863E-5	0.0	0.0	0.0
7	0.0	6.506210503235863E-5	0.0	2.6024842012943456E-5	0.0
8	0.0	7.807452603883036E-5	0.0	5.204968402588691E-5	0.0
9	0.0	1.43136631071189E-4	0.0	7.807452603883036E-5	0.0
10-11	0.0	1.43136631071189E-4	0.0	2.667546306326704E-4	0.0
12-13	0.0	1.43136631071189E-4	0.0	5.985713662976994E-4	0.0
14-15	0.0	2.1470494660678351E-4	0.0	0.001476909784234541	6.506210503235864E-6
16-17	0.0	2.342235781164911E-4	0.0	0.0028432139899140724	1.3012421006471728E-5
18-19	0.0	2.342235781164911E-4	0.0	0.005933663978951108	1.3012421006471728E-5
20-21	0.0	2.342235781164911E-4	0.0	0.013578461320253248	1.3012421006471728E-5
22-23	0.0	2.7326084113590626E-4	0.0	0.027827062322339786	2.6024842012943453E-5
24-25	0.0	3.2531052516179316E-4	0.0	0.03987656417433261	3.903726301941518E-5
26-27	0.0	3.5133536717473667E-4	0.0	0.051639792764183054	3.903726301941518E-5
28-29	0.0	3.5133536717473667E-4	0.0	0.06769061407566593	3.903726301941518E-5
30-31	0.0	4.2940989321356703E-4	0.0	0.06956440270059785	3.903726301941518E-5
32-33	0.0	4.359161037168029E-4	0.0	0.07247918500604753	3.903726301941518E-5
34-35	0.0	4.944719982459256E-4	0.0	0.07698148267428674	3.903726301941518E-5
36-37	0.0	5.465216822718125E-4	0.0	0.08618126432586226	3.903726301941518E-5
38-39	0.0	5.465216822718125E-4	0.0	0.14810086968515795	3.903726301941518E-5
40-41	0.0	6.376086293171146E-4	0.0	0.19726179624760815	3.903726301941518E-5
42-43	0.0	7.221893658591809E-4	0.0	0.21199836303743738	3.903726301941518E-5
44-45	0.0	8.067701024012472E-4	0.0	0.23241485159659153	4.554347352265104E-5
46-47	0.0	8.067701024012472E-4	0.0	0.25830956939947025	5.204968402588691E-5
48-49	0.0	8.262887339109547E-4	0.0	0.2847638213056273	6.506210503235863E-5
50-51	0.0	9.499067334724361E-4	0.0	0.3321550586111973	6.506210503235863E-5
52-53	0.0	9.499067334724361E-4	0.0	0.6210633360073858	6.506210503235863E-5
54-55	0.0	9.95450206995087E-4	0.0	1.0107202830461817	6.506210503235863E-5
56-57	0.0	0.0010409936805177381	0.0	1.4504295074863711	6.506210503235863E-5
58-59	0.0	0.0010409936805177381	0.0	1.910236416171056	6.506210503235863E-5
60-61	0.0	0.001047499891020974	0.0	2.0238738888205736	6.506210503235863E-5
62-63	0.0	0.00105400610152421	0.0	2.0286689659614585	6.506210503235863E-5
64-65	0.0	0.0011841303115889272	0.0	2.0331322263666785	6.506210503235863E-5
66-67	0.0	0.0011841303115889272	0.0	2.037426325298814	6.506210503235863E-5
68-69	0.0	0.001190636522092163	0.0	2.0416813869679302	6.506210503235863E-5
70-71	0.0	0.0012036489430986346	0.0	2.04525980274471	9.10869470453021E-5
72-73	0.0	0.0012101551536018707	0.0	2.0485389328383405	9.10869470453021E-5
74-75	0.0	0.0012296737851115783	0.0	2.0510503300925897	9.10869470453021E-5
76-77	0.0	0.0012752172586342292	0.0	2.0536463080833807	9.10869470453021E-5
78-79	0.0	0.0013012421006471727	0.0	2.0552208110251637	9.10869470453021E-5
80-81	0.0	0.0013012421006471727	0.0	2.055799863759952	9.759315754853796E-5
82-83	0.0	0.0013142545216536445	0.0	2.056489522073295	1.0409936805177383E-4
84-85	0.0	0.0013272669426601163	0.0	2.0568603760719792	1.0409936805177383E-4
86-87	0.0	0.0013272669426601163	0.0	2.0570230313345603	1.0409936805177383E-4
88	0.0	0.0013923290476924748	0.0	2.0570295375450636	1.0409936805177383E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	4600	0.0	34.847775	94
TATCTCG	5355	0.0	32.168518	94
GGTATCA	23430	0.0	32.15833	1
GTATCAA	33820	0.0	27.159552	1
ATCAACG	34260	0.0	25.76815	3
TCAACGC	35270	0.0	25.017897	4
CAACGCA	35840	0.0	24.584356	5
AACGCAG	36045	0.0	24.482063	6
ACGCAGA	36435	0.0	24.243916	7
CGCTATC	8140	0.0	23.023972	94
GTACATG	29915	0.0	22.62464	1
TATCAAC	40170	0.0	22.606155	2
ACATGGG	28570	0.0	22.470053	3
TACATGG	29590	0.0	22.402277	2
CGCAGAG	41620	0.0	21.181759	8
GAGTACT	23950	0.0	21.122192	12-13
GCAGAGT	46305	0.0	18.98209	9
CAGAGTA	41195	0.0	18.978886	10-11
GTACTTT	26580	0.0	18.926947	14-15
AGAGTAC	37095	0.0	18.734072	10-11
>>END_MODULE