FastQCFastQC Report
Fri 27 May 2016
SRR2138913_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138913_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6730217
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96250.052.8624341
GTATCAA181450.039.458661
TCAACGC220950.031.7739014
ATCAACG221700.031.6860453
CAACGCA225500.031.1523195
TATCAAC229500.030.7800432
AACGCAG231050.030.4083086
ACGCAGA264850.026.5440547
CGCAGAG265850.026.4605838
GTGGTAT45900.024.938851
TGGTATC45300.023.8279612
GCAGAGT308200.022.6692479
GTACATG204300.021.7941881
TACATGG200800.021.5454752
ACATGGG201050.020.8041273
GAGTACT206500.020.64644212-13
CAGAGTA304100.019.73263410-11
AGAGTAC284550.019.7268410-11
GTACTTT229150.018.5095314-15
AGTACTT220900.018.3153212-13
Produced by FastQC (version 0.11.5)