##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138913_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6730217
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69597919353863	33.0	33.0	33.0	33.0	33.0
2	32.69769904893111	33.0	33.0	33.0	33.0	33.0
3	32.706420164461264	33.0	33.0	33.0	33.0	33.0
4	36.63467240357926	37.0	37.0	37.0	37.0	37.0
5	36.64614870516062	37.0	37.0	37.0	37.0	37.0
6	36.58324360120929	37.0	37.0	37.0	37.0	37.0
7	36.5621503734575	37.0	37.0	37.0	37.0	37.0
8	36.55848585565666	37.0	37.0	37.0	37.0	37.0
9	36.59895513027292	37.0	37.0	37.0	37.0	37.0
10-11	36.57703340026035	37.0	37.0	37.0	37.0	37.0
12-13	36.56837862731618	37.0	37.0	37.0	37.0	37.0
14-15	38.671807536072016	40.0	37.0	40.0	37.0	40.0
16-17	38.60863617027505	40.0	37.0	40.0	37.0	40.0
18-19	38.63191699465263	40.0	37.0	40.0	37.0	40.0
20-21	38.647441132845266	40.0	37.0	40.0	37.0	40.0
22-23	38.64517054916216	40.0	37.0	40.0	37.0	40.0
24-25	38.64207129518395	40.0	37.0	40.0	37.0	40.0
26-27	38.557023158933745	40.0	37.0	40.0	37.0	40.0
28-29	38.44397738702866	40.0	37.0	40.0	37.0	40.0
30-31	38.31311841134572	40.0	37.0	40.0	37.0	40.0
32-33	38.199748248077455	40.0	37.0	40.0	37.0	40.0
34-35	38.17037943772915	40.0	37.0	40.0	37.0	40.0
36-37	38.151297241966816	40.0	37.0	40.0	37.0	40.0
38-39	38.108048218381924	40.0	37.0	40.0	37.0	40.0
40-41	38.0806794914526	40.0	37.0	40.0	37.0	40.0
42-43	38.02142625406749	40.0	37.0	40.0	33.0	40.0
44-45	37.92229243108341	40.0	37.0	40.0	33.0	40.0
46-47	37.81465988865611	40.0	37.0	40.0	33.0	40.0
48-49	37.749443840708665	40.0	37.0	40.0	33.0	40.0
50-51	37.723450222685116	40.0	37.0	40.0	33.0	40.0
52-53	37.659822696265394	40.0	37.0	40.0	33.0	40.0
54-55	37.545450096472365	40.0	37.0	40.0	33.0	40.0
56-57	37.37219525635355	40.0	37.0	40.0	33.0	40.0
58-59	37.18378424340693	37.0	37.0	40.0	33.0	40.0
60-61	37.01076818134991	37.0	37.0	40.0	33.0	40.0
62-63	36.86495891003847	37.0	37.0	40.0	33.0	40.0
64-65	36.69331229567817	37.0	37.0	40.0	33.0	40.0
66-67	36.479327041957504	37.0	37.0	40.0	33.0	40.0
68-69	36.27240166007795	37.0	37.0	40.0	33.0	40.0
70-71	36.04880611971929	37.0	37.0	38.5	33.0	40.0
72-73	35.756651062754926	37.0	35.0	37.0	33.0	40.0
74-75	35.39984396806564	37.0	33.0	37.0	33.0	40.0
76-77	33.33289421714956	35.0	33.0	37.0	27.0	37.0
78-79	34.97106186745733	37.0	33.0	37.0	33.0	37.0
80-81	35.17606667837549	37.0	33.0	37.0	33.0	37.0
82-83	34.972089465543675	37.0	33.0	37.0	33.0	37.0
84-85	34.81738349779262	37.0	33.0	37.0	33.0	37.0
86-87	34.7063286974013	37.0	33.0	37.0	33.0	37.0
88-89	34.589278903741146	37.0	33.0	37.0	33.0	37.0
90-91	34.514315423584534	37.0	33.0	37.0	33.0	37.0
92-93	34.468192473877195	37.0	33.0	37.0	33.0	37.0
94-95	34.422831293168215	37.0	33.0	37.0	33.0	37.0
96-97	34.43499015679155	37.0	33.0	37.0	33.0	37.0
98-99	34.461742245897895	37.0	33.0	37.0	33.0	37.0
100	34.41357873871586	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	30.0
21	146.0
22	723.0
23	2149.0
24	5053.0
25	10544.0
26	18798.0
27	30944.0
28	46715.0
29	66904.0
30	92777.0
31	124107.0
32	166285.0
33	226853.0
34	316648.0
35	477922.0
36	864911.0
37	2057504.0
38	2170615.0
39	50584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.831459520547405	20.223701553753763	14.566781427701365	23.378057497997464
2	14.406578314509726	20.839991839661533	42.7231162389101	22.030313606918636
3	18.300898709623258	26.145696899277215	30.498729236330842	25.05467515476869
4	12.323480803070689	17.926762242584452	39.39063183252486	30.359125121819996
5	13.09864154454455	37.74478594078022	35.78056992813159	13.376002586543642
6	29.467355955981805	38.152484533559615	18.915660520307146	13.464498990151434
7	26.122931846031115	32.92657577014233	23.333868729641257	17.616623654185297
8	23.82119328396098	37.35622491815643	21.210995128388877	17.611586669493718
9	25.31326998817423	17.259621792283962	21.581666683258504	35.8454415362833
10-11	23.99743871366905	27.07895382790319	29.000899228731576	19.92270822969618
12-13	24.836914035073903	24.548863974246824	29.384635319112956	21.229586671566317
14-15	21.87924995583352	25.92186106332084	27.30297552070015	24.89591346014549
16-17	21.11840524607156	29.12166873668412	27.96572532505267	21.794200692191648
18-19	21.112016150445076	28.025626811141453	30.066905717898845	20.79545132051463
20-21	21.934966644846686	27.219830344991237	30.08061060885032	20.764592401311756
22-23	21.590775436043973	27.041966871141664	30.03358119435284	21.333676498461514
24-25	21.36272686569105	27.198846651890634	30.107245768301134	21.33118071411718
26-27	21.30046708140246	27.373082840584615	30.013509255211336	21.312940822801583
28-29	21.223019094668313	27.476570008912287	30.04607272882242	21.254338167596977
30-31	21.55733006711632	27.30779874081795	29.933309524612504	21.201561667453216
32-33	21.29991235282255	27.559600378989018	29.83859852819566	21.30188873999277
34-35	21.41362431086835	27.605281868103404	29.774396824557915	21.206696996470328
36-37	21.46339703339171	27.51226960131916	29.724173451272733	21.300159914016398
38-39	21.278669921086404	27.69514113001445	29.761817318820068	21.26437163007908
40-41	21.575845572178885	27.62591282563505	29.53328693630606	21.26495466588001
42-43	21.509338104530194	27.683832490675293	29.324469349236782	21.482360055557727
44-45	21.54233411294	27.866387552770544	29.131294772222464	21.459983562066988
46-47	21.630276528812733	27.827102439358818	28.795370963849305	21.74725006797914
48-49	21.612128035574873	27.932196440179336	28.73947685783692	21.716198666408868
50-51	21.627814714974683	28.10817765825137	28.549835240300748	21.714172386473198
52-53	21.810835869174213	28.036439317477207	28.37433637760126	21.778388435747313
54-55	21.83682567918779	27.89705762206549	28.423296028619045	21.84282067012768
56-57	21.811759702157488	28.025334809588102	28.361659409191642	21.801246079062764
58-59	21.75805358296191	28.08392655095231	28.383603681473407	21.77441618461237
60-61	21.838814308703977	27.98439348204045	28.393577588588524	21.783214620667046
62-63	21.770828590024173	28.07296269698567	28.321694752314137	21.834513960676023
64-65	21.745615612211793	28.089167783853362	28.270971190263083	21.894245413671758
66-67	21.91763508737432	28.134335089089564	28.03541735724287	21.912612466293247
68-69	21.788194200453923	28.116264393006603	28.169759975053584	21.92578143148589
70-71	21.81918382541026	27.84606522159579	28.206840760065965	22.127910192927985
72-73	21.9565738978601	27.897361815094662	28.071443077303154	22.074621209742084
74-75	21.72411975298385	28.142836839532702	28.0696581155129	22.06338529197054
76-77	21.90557759966376	28.062751425788456	27.942913928424872	22.08875704612291
78-79	21.966784201299703	28.068120429056286	27.837740461603154	22.127354908040857
80-81	21.9269093830807	28.037198872485746	27.865166622641986	22.170725121791563
82-83	21.986638963427488	28.001032794166626	27.968849135998575	22.043479106407318
84-85	22.173897482303513	27.964798050745614	27.843083411849733	22.018221055101144
86-87	21.923057398169203	28.008154144766088	27.96405501840554	22.104733438659174
88-89	22.057853293501005	28.05524403422408	27.875640109831075	22.01126256244384
90-91	22.12536446014005	27.984614883850185	27.89829045348638	21.99173020252339
92-93	22.089392593327993	28.040756332886335	27.881051747132762	21.98879932665291
94-95	21.95373579147805	28.117424694879883	27.86604706410527	22.062792449536797
96-97	22.03316187415619	28.186641929184596	28.012834813178305	21.767361383480914
98-99	23.187551219273534	30.098524477206656	28.120583930296938	18.59334037322287
100	22.135777554184497	31.05631579408641	21.99597831358936	24.811928338139733
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3309.0
1	2633.0
2	2728.0
3	3744.5
4	4796.0
5	7326.5
6	9667.5
7	11739.5
8	13632.0
9	14183.0
10	13186.0
11	11566.5
12	11780.5
13	14659.5
14	22328.0
15	32970.5
16	41592.5
17	46594.0
18	47257.0
19	44281.5
20	39839.5
21	35061.0
22	31908.0
23	32540.5
24	36639.5
25	44552.0
26	56346.5
27	69613.5
28	81819.0
29	95467.5
30	109142.0
31	122680.0
32	139189.5
33	156050.0
34	173724.5
35	190003.0
36	207469.5
37	223729.0
38	232864.0
39	239739.5
40	251347.0
41	262344.0
42	268809.0
43	276577.5
44	281621.5
45	285148.0
46	286169.5
47	284088.5
48	281611.0
49	273984.0
50	263257.5
51	250011.0
52	233050.5
53	215055.5
54	196475.0
55	176631.5
56	156472.5
57	136686.5
58	118993.5
59	103102.0
60	85435.5
61	68278.0
62	53201.0
63	40195.5
64	29840.5
65	22036.0
66	16419.5
67	12366.5
68	9188.0
69	6380.5
70	4330.5
71	3010.5
72	1964.5
73	1198.0
74	784.0
75	516.0
76	361.5
77	254.0
78	184.5
79	148.0
80	108.0
81	72.0
82	55.5
83	39.5
84	23.5
85	15.5
86	8.0
87	4.0
88	2.5
89	1.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0015452696398942263
3	1.7830034306471842E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	7.429180961029934E-5
12-13	0.007191447170276976
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.4859919624180746E-5
24-25	2.452344991731882E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	5.980306402493608E-4
36-37	0.025108251210303204
38-39	0.12066573136915834
40-41	0.1421342910576346
42-43	0.030904062936835702
44-45	0.002198724387446494
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.006518550005972642
64-65	0.07258883385033518
66-67	0.17906514037214386
68-69	0.18485438405746873
70-71	0.0690794026585179
72-73	0.0019261037726092131
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	75.0
20-21	426.0
22-23	979.0
24-25	1962.0
26-27	2977.0
28-29	5158.0
30-31	11179.0
32-33	14387.0
34-35	19755.0
36-37	27418.0
38-39	31544.0
40-41	34422.0
42-43	41142.0
44-45	49886.0
46-47	49064.0
48-49	42129.0
50-51	34828.0
52-53	32486.0
54-55	34360.0
56-57	35322.0
58-59	35625.0
60-61	33602.0
62-63	36857.0
64-65	39172.0
66-67	45345.0
68-69	43799.0
70-71	44731.0
72-73	43225.0
74-75	42796.0
76-77	38315.0
78-79	42531.0
80-81	44657.0
82-83	44571.0
84-85	48724.0
86-87	54951.0
88-89	60517.0
90-91	65123.0
92-93	68456.0
94-95	83162.0
96-97	229675.0
98-99	436747.0
100-101	4678137.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.3507689363897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.66072755396122	74.78600543636382
2	8.038601081905583	13.561243648632601
3	1.925438161253449	4.872365683235902
4	0.6619814325335102	2.2335457142325743
5	0.3110056864260901	1.3116784396815198
6	0.14221381734232452	0.7197506907722607
7	0.0855155223677718	0.504931004740301
8	0.04398400961132072	0.2968068025296366
9	0.03310175112694428	0.2512942344628879
>10	0.09486448784127213	1.196812358072003
>50	0.0017889472000016803	0.10176216964631
>100	6.667865203334369E-4	0.09221421478380604
>500	8.307143853676547E-5	0.04538290772505414
>1k	2.7690471670978985E-5	0.02620669512132717
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.943344768823947E-5	0.0	0.0	0.0
3	0.0	5.943344768823947E-5	0.0	0.0	0.0
4	0.0	1.0400853345441908E-4	0.0	0.0	0.0
5	0.0	1.0400853345441908E-4	0.0	0.0	0.0
6	0.0	1.0400853345441908E-4	0.0	0.0	0.0
7	0.0	1.0400853345441908E-4	0.0	0.0	0.0
8	0.0	1.0400853345441908E-4	0.0	0.0	0.0
9	0.0	2.5259215267501775E-4	0.0	0.0	0.0
10-11	0.0	2.5259215267501775E-4	0.0	2.9716723844119736E-5	0.0
12-13	0.0	2.5259215267501775E-4	0.0	2.9716723844119736E-5	0.0
14-15	0.0	3.34313143246347E-4	0.0	2.9716723844119736E-5	1.4858361922059868E-5
16-17	0.0	3.4174232420737695E-4	0.0	2.9716723844119736E-5	3.714590480514967E-5
18-19	0.0	3.640298670904668E-4	0.0	2.9716723844119736E-5	4.4575085766179605E-5
20-21	0.0	3.714590480514967E-4	0.0	2.9716723844119736E-5	4.4575085766179605E-5
22-23	0.0	4.3832167670076615E-4	0.0	3.714590480514967E-5	7.429180961029934E-5
24-25	0.0	5.200426672720954E-4	0.0	5.943344768823947E-5	1.0400853345441908E-4
26-27	0.0	5.349010291941553E-4	0.0	1.560128001816286E-4	1.0400853345441908E-4
28-29	0.0	5.349010291941553E-4	0.0	6.389095626485743E-4	1.1143771441544901E-4
30-31	0.0	5.794761149603349E-4	0.0	0.0019835913165949922	1.1886689537647894E-4
32-33	0.0	5.794761149603349E-4	0.0	0.004821538443708428	1.1886689537647894E-4
34-35	0.0	7.132013722588737E-4	0.0	0.008825866981703562	1.3372525729853882E-4
36-37	0.0	8.543558105184425E-4	0.0	0.014940082912631197	1.3372525729853882E-4
38-39	0.0	8.766433534015322E-4	0.0	0.030756809178663927	1.4115443825956877E-4
40-41	0.0	9.509351630118315E-4	0.0	0.04742046207425407	1.485836192205987E-4
42-43	0.0	9.509351630118315E-4	0.0	0.05765787343855332	1.485836192205987E-4
44-45	0.0	0.0010549436964662506	0.0	0.06955199215716223	1.560128001816286E-4
46-47	0.0	0.0011589522299206697	0.0	0.08024258356008432	1.7830034306471842E-4
48-49	0.0	0.0011663814108816995	0.0	0.0917280973258366	2.377337907529579E-4
50-51	0.0	0.0013001066681802384	0.0	0.10343648652041977	2.377337907529579E-4
52-53	0.0	0.0013223942110633282	0.0	0.12975807466534883	2.600213336360477E-4
54-55	0.0	0.0013223942110633282	0.0	0.16173326952162165	2.823088765191375E-4
56-57	0.0	0.0013223942110633282	0.0	0.19288382529122017	2.823088765191375E-4
58-59	0.0	0.0013223942110633282	0.0	0.21223684169470314	2.823088765191375E-4
60-61	0.0	0.001366969296829508	0.0	0.22872962342818962	2.971672384411974E-4
62-63	0.0	0.0013818276587515678	0.0	0.2415821064907714	2.971672384411974E-4
64-65	0.0	0.0015452696398942263	0.0	0.24539327632377977	2.971672384411974E-4
66-67	0.0	0.0015452696398942263	0.0	0.2492267336996712	2.971672384411974E-4
68-69	0.0	0.0015601280018162863	0.0	0.2535059419332244	2.971672384411974E-4
70-71	0.0	0.001574986363738346	0.0	0.2574359786616093	3.120256003632572E-4
72-73	0.0	0.001574986363738346	0.0	0.2604150802269823	3.268839622853171E-4
74-75	0.0	0.0016121322685434957	0.0	0.2626512636962523	3.268839622853171E-4
76-77	0.0	0.001693853259114825	0.0	0.2647685802701458	3.268839622853171E-4
78-79	0.0	0.0017235699829589448	0.0	0.2661281203860143	3.268839622853171E-4
80-81	0.0	0.0017235699829589448	0.0	0.26661101714848123	3.34313143246347E-4
82-83	0.0	0.0017235699829589448	0.0	0.2670270512822989	3.4174232420737695E-4
84-85	0.0	0.0017384283448810046	0.0	0.26722763916824677	3.4174232420737695E-4
86-87	0.0	0.0017384283448810046	0.0	0.26735393524458423	3.4174232420737695E-4
88	0.0	0.0018721536021795433	0.0	0.26736136442554526	3.4174232420737695E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9625	0.0	52.862434	1
GTATCAA	18145	0.0	39.45866	1
TCAACGC	22095	0.0	31.773901	4
ATCAACG	22170	0.0	31.686045	3
CAACGCA	22550	0.0	31.152319	5
TATCAAC	22950	0.0	30.780043	2
AACGCAG	23105	0.0	30.408308	6
ACGCAGA	26485	0.0	26.544054	7
CGCAGAG	26585	0.0	26.460583	8
GTGGTAT	4590	0.0	24.93885	1
TGGTATC	4530	0.0	23.827961	2
GCAGAGT	30820	0.0	22.669247	9
GTACATG	20430	0.0	21.794188	1
TACATGG	20080	0.0	21.545475	2
ACATGGG	20105	0.0	20.804127	3
GAGTACT	20650	0.0	20.646442	12-13
CAGAGTA	30410	0.0	19.732634	10-11
AGAGTAC	28455	0.0	19.72684	10-11
GTACTTT	22915	0.0	18.50953	14-15
AGTACTT	22090	0.0	18.31532	12-13
>>END_MODULE