FastQCFastQC Report
Fri 27 May 2016
SRR2138912_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138912_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8161776
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145550.048.604221
GTATCAA252050.036.221311
ATCAACG310650.028.8452643
TCAACGC312850.028.5869244
TATCAAC320250.028.2104342
CAACGCA318000.028.1375645
AACGCAG319300.028.0422276
ACGCAGA359000.024.9532327
CGCAGAG363750.024.6154848
TACATGG260000.022.0667612
GCAGAGT408100.021.7919529
ACATGGG261000.021.385283
GTACATG293550.020.4585951
GAGTACT276300.020.3752712-13
GTGGTAT72700.020.3544861
CATGGGG179250.019.817424
TGGTATC69700.019.5544782
CAGAGTA408550.019.41637610-11
AGAGTAC386450.018.9141710-11
GTACTAG25800.018.6153721
Produced by FastQC (version 0.11.5)