##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138912_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8161776
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69217104218494	33.0	33.0	33.0	33.0	33.0
2	32.69620533570144	33.0	33.0	33.0	33.0	33.0
3	32.70491165158172	33.0	33.0	33.0	33.0	33.0
4	36.63308096179067	37.0	37.0	37.0	37.0	37.0
5	36.64582990270745	37.0	37.0	37.0	37.0	37.0
6	36.58614240332006	37.0	37.0	37.0	37.0	37.0
7	36.56242072803762	37.0	37.0	37.0	37.0	37.0
8	36.55954292301087	37.0	37.0	37.0	37.0	37.0
9	36.600475313216144	37.0	37.0	37.0	37.0	37.0
10-11	36.58039083650421	37.0	37.0	37.0	37.0	37.0
12-13	36.56840998821825	37.0	37.0	37.0	37.0	37.0
14-15	38.67170784887995	40.0	37.0	40.0	37.0	40.0
16-17	38.61130506399587	40.0	37.0	40.0	37.0	40.0
18-19	38.638438251674636	40.0	37.0	40.0	37.0	40.0
20-21	38.65342515111898	40.0	37.0	40.0	37.0	40.0
22-23	38.65149606995273	40.0	37.0	40.0	37.0	40.0
24-25	38.64878859284589	40.0	37.0	40.0	37.0	40.0
26-27	38.564875664113295	40.0	37.0	40.0	37.0	40.0
28-29	38.448903245110216	40.0	37.0	40.0	37.0	40.0
30-31	38.3120803408606	40.0	37.0	40.0	37.0	40.0
32-33	38.19537258413628	40.0	37.0	40.0	37.0	40.0
34-35	38.16899772341141	40.0	37.0	40.0	37.0	40.0
36-37	38.156159978887615	40.0	37.0	40.0	37.0	40.0
38-39	38.107529657661196	40.0	37.0	40.0	37.0	40.0
40-41	38.07945209611498	40.0	37.0	40.0	37.0	40.0
42-43	38.0246163163127	40.0	37.0	40.0	35.0	40.0
44-45	37.92200781161301	40.0	37.0	40.0	33.0	40.0
46-47	37.821822054415634	40.0	37.0	40.0	33.0	40.0
48-49	37.75337416071757	40.0	37.0	40.0	33.0	40.0
50-51	37.72813050924685	40.0	37.0	40.0	33.0	40.0
52-53	37.66289399793337	40.0	37.0	40.0	33.0	40.0
54-55	37.54608867037369	40.0	37.0	40.0	33.0	40.0
56-57	37.372833216351154	40.0	37.0	40.0	33.0	40.0
58-59	37.17660505095215	37.0	37.0	40.0	33.0	40.0
60-61	37.00994265023256	37.0	37.0	40.0	33.0	40.0
62-63	36.86641521249574	37.0	37.0	40.0	33.0	40.0
64-65	36.68833662938679	37.0	37.0	40.0	33.0	40.0
66-67	36.480942499502255	37.0	37.0	40.0	33.0	40.0
68-69	36.27309533128549	37.0	37.0	40.0	33.0	40.0
70-71	36.05855685923218	37.0	37.0	38.5	33.0	40.0
72-73	35.77502380652359	37.0	35.0	37.0	33.0	40.0
74-75	35.42128001804085	37.0	33.0	37.0	33.0	40.0
76-77	33.32294929071794	35.0	33.0	37.0	27.0	37.0
78-79	34.98621244736665	37.0	33.0	37.0	33.0	37.0
80-81	35.205105601585004	37.0	33.0	37.0	33.0	37.0
82-83	34.998059740567314	37.0	33.0	37.0	33.0	37.0
84-85	34.842871294709674	37.0	33.0	37.0	33.0	37.0
86-87	34.73115787513092	37.0	33.0	37.0	33.0	37.0
88-89	34.61555836465433	37.0	33.0	37.0	33.0	37.0
90-91	34.54422363011504	37.0	33.0	37.0	33.0	37.0
92-93	34.499612705797944	37.0	33.0	37.0	33.0	37.0
94-95	34.45070777791617	37.0	33.0	37.0	33.0	37.0
96-97	34.46130487083718	37.0	33.0	37.0	33.0	37.0
98-99	34.485130089028075	37.0	33.0	37.0	33.0	37.0
100	34.428823454450594	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	7.0
20	29.0
21	192.0
22	857.0
23	2462.0
24	6076.0
25	12612.0
26	23022.0
27	37363.0
28	56745.0
29	81035.0
30	109466.0
31	147591.0
32	197706.0
33	269130.0
34	379056.0
35	572259.0
36	1047711.0
37	2540050.0
38	2610821.0
39	67584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.53396270615611	20.729887710713943	14.70602721760558	23.030122365524367
2	14.316527379080531	20.736931780101845	43.147262729072835	21.799278111744794
3	17.885973263152476	26.74831562271419	30.734534298320092	24.631176815813234
4	11.95123463324649	17.78552854182717	40.129979063380325	30.133257761546016
5	12.622693884272246	38.33954766707638	36.059590461683825	12.978167986967543
6	29.130130501008605	38.9123151627783	18.74692468894025	13.210629647272848
7	25.770530825643828	33.25036119589658	23.719469880084922	17.259638098374666
8	23.97438988769111	37.25558015804403	21.187030861910447	17.58299909235441
9	25.325921711156983	17.080571679497208	21.84180256846059	35.751704040885215
10-11	23.962921541321776	27.343938952487534	29.126645751279074	19.566493754911615
12-13	24.804804168229456	24.615462281540022	29.389120153585953	21.190613396644572
14-15	21.780290221147947	25.95841885393571	27.635327164087816	24.625963760828526
16-17	20.936932108893945	29.00305031649974	28.257642699334067	21.802374875272246
18-19	20.794499873556934	27.97651516042587	30.483855474592787	20.745129491424414
20-21	21.955742649428704	27.425193677752397	30.279917704453418	20.339145968365482
22-23	21.379013041801883	27.095511971032458	30.3303611488716	21.19511383829406
24-25	21.01515453552325	27.229930285790694	30.60157988969684	21.153335288989215
26-27	21.052730306363237	27.511886635683336	30.304904266315564	21.13047879163786
28-29	20.941082182619553	27.84026151756024	30.280769848635806	20.937886451184404
30-31	21.416057283314572	27.50835748112554	30.121465600611984	20.9541196349479
32-33	21.194734384517353	27.763392324394193	29.984587292649607	21.05728599843885
34-35	21.151041497282208	27.867129805306632	29.988959244486736	20.992869452924424
36-37	21.33077503370808	27.58977970284855	29.996319150810375	21.083126112632993
38-39	21.09308422355024	27.8769360064767	29.996663107605464	21.033316662367596
40-41	21.500587821275026	27.828028413062917	29.737732076367106	20.93365168929495
42-43	21.450623412096036	27.911452266578628	29.472850653531573	21.165073667793767
44-45	21.4630345469504	28.226545683331334	29.139852952351642	21.170566817366623
46-47	21.549195435341257	27.986907829527226	28.7196595826051	21.74423715252642
48-49	21.607538846947275	28.101470660434508	28.75982022039869	21.531170272219534
50-51	21.36835565505097	28.32432982149782	28.594269788068587	21.713044735382624
52-53	21.650608996781685	28.294346009919447	28.295167674314143	21.759877318984724
54-55	21.816893750689133	28.059006840249822	28.359812375276196	21.764287033784854
56-57	21.777834261658956	28.250658531229977	28.219868517213964	21.7516386898971
58-59	21.63448411307582	28.254295549051456	28.492437295506484	21.618783042366235
60-61	21.66179859182844	28.31221609847992	28.279430832297326	21.746554477394316
62-63	21.746375226884147	28.20889944666597	28.196467162650546	21.84825816379934
64-65	21.60294106304615	28.35270998164745	28.20058393129437	21.843765024012033
66-67	21.847349103229654	28.51421002077984	27.79691929926655	21.841521576723956
68-69	21.802766699384293	28.273937806796535	28.125955367434358	21.797340126384817
70-71	21.81513155644678	27.81576877314186	28.182957624258986	22.18614204615238
72-73	21.93403238347894	27.930625078788868	28.15550105256347	21.979841485168727
74-75	21.572923022945226	28.25142405239	28.09941455116362	22.07623837350115
76-77	21.7558931033418	28.23424173982557	27.852779566363683	22.15708559046894
78-79	21.945196997775867	28.298295020878893	27.717430847125392	22.03907713421985
80-81	21.829340607594357	28.093202116501637	27.874747072963423	22.20271020294058
82-83	22.016983350451277	28.051006180517046	27.995297623437565	21.936712845594112
84-85	22.411941031064252	27.93226428908011	27.722776621269873	21.933018058585763
86-87	21.712836459219776	28.22959217117956	27.895989743305066	22.161581626295597
88-89	22.082346347273653	28.144965110160925	27.885876606274113	21.886811936291313
90-91	22.126140796015356	28.1715093723809	27.930102020747515	21.77224781085623
92-93	22.161997721792115	28.15123483714575	27.75293455546237	21.933832885599756
94-95	21.879011385378373	28.295546125051136	27.77633790092927	22.04910458864122
96-97	21.88897026743711	28.36710367605108	28.060945732791893	21.682980323719917
98-99	23.00783757203013	30.21890459939186	28.2395111150268	18.533746713551214
100	22.254904192399163	31.257497501057017	21.92538605241211	24.5622122541317
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2834.0
1	2462.5
2	3357.5
3	4916.0
4	6889.5
5	11858.5
6	15925.0
7	18225.0
8	19118.0
9	17847.0
10	15512.5
11	13292.0
12	14258.5
13	19413.5
14	31646.5
15	48554.5
16	62227.0
17	69908.0
18	71141.0
19	66689.5
20	59529.0
21	51686.0
22	46512.0
23	47185.5
24	52888.0
25	64240.0
26	82931.5
27	102611.5
28	115417.0
29	128409.5
30	139894.0
31	150327.5
32	168936.0
33	190689.5
34	215146.0
35	237782.5
36	263386.5
37	285127.5
38	286535.0
39	288114.5
40	303067.0
41	312528.0
42	314323.0
43	319679.5
44	325290.5
45	329779.0
46	331070.5
47	331834.5
48	331717.0
49	326631.5
50	315081.0
51	297842.0
52	279755.5
53	258180.0
54	234072.5
55	211717.0
56	189713.5
57	168375.0
58	148065.0
59	129547.0
60	106336.5
61	79821.0
62	59157.0
63	43384.5
64	31108.5
65	21598.5
66	15053.0
67	11127.0
68	8176.0
69	5891.0
70	4446.0
71	3130.5
72	2161.0
73	1518.5
74	1058.5
75	646.0
76	410.5
77	264.0
78	173.0
79	117.5
80	85.5
81	67.0
82	54.0
83	34.0
84	15.5
85	8.0
86	8.0
87	7.5
88	6.0
89	4.5
90	2.5
91	1.0
92	0.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0015315294122259665
3	7.351341178684639E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	2.4504470595615466E-5
12-13	0.00751062023755614
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.8380726607278133E-5
24-25	2.512482441730936E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	6.294505470634929E-4
36-37	0.025137682848154583
38-39	0.1219650053009734
40-41	0.14357228403936953
42-43	0.031795952275873604
44-45	0.0022435585627408935
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.007065282945777043
64-65	0.07436024934582383
66-67	0.18320353833416714
68-69	0.1908512623937136
70-71	0.0718532947496038
72-73	0.0023078329873559337
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	115.0
20-21	648.0
22-23	1316.0
24-25	2389.0
26-27	4229.0
28-29	7764.0
30-31	16493.0
32-33	20269.0
34-35	26905.0
36-37	35669.0
38-39	39076.0
40-41	46715.0
42-43	54852.0
44-45	64990.0
46-47	65925.0
48-49	53537.0
50-51	43604.0
52-53	39738.0
54-55	40766.0
56-57	43729.0
58-59	48082.0
60-61	47723.0
62-63	49294.0
64-65	51452.0
66-67	58984.0
68-69	55748.0
70-71	56748.0
72-73	57304.0
74-75	55183.0
76-77	49734.0
78-79	53873.0
80-81	60067.0
82-83	56617.0
84-85	61771.0
86-87	70344.0
88-89	77051.0
90-91	82331.0
92-93	85648.0
94-95	101836.0
96-97	271286.0
98-99	522612.0
100-101	5579359.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.63401083624132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.96307718015682	66.73658465320943
2	8.827589006140899	13.706422811212546
3	2.5868085124390747	6.024729602579292
4	1.128647421472779	3.504857045956542
5	0.5579247021892563	2.1656966187783717
6	0.29846667915045716	1.390269924205413
7	0.18832079348447917	1.0234068965444532
8	0.11926157698571699	0.7407003648045105
9	0.07422616333732661	0.5186227291976369
>10	0.24792016563382496	2.884732725481524
>50	0.00489480790836044	0.26416318119410526
>100	0.002197914041549631	0.3432122748358807
>500	3.657924155855441E-4	0.20704673227777767
>1k	2.660307945623783E-4	0.35221173976344594
>5k	3.3253849320296616E-5	0.13734269995906384
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.2252235297807731E-5	0.0
5	0.0	0.0	0.0	1.2252235297807731E-5	0.0
6	0.0	0.0	0.0	1.2252235297807731E-5	0.0
7	0.0	0.0	0.0	4.9008941191230925E-5	0.0
8	0.0	0.0	0.0	4.9008941191230925E-5	0.0
9	0.0	0.0	0.0	4.9008941191230925E-5	0.0
10-11	0.0	0.0	0.0	5.513505884013479E-5	0.0
12-13	0.0	0.0	0.0	6.126117648903866E-5	0.0
14-15	0.0	0.0	0.0	6.126117648903866E-5	0.0
16-17	0.0	0.0	0.0	6.126117648903866E-5	0.0
18-19	0.0	0.0	0.0	6.126117648903866E-5	0.0
20-21	0.0	0.0	0.0	9.801788238246185E-5	0.0
22-23	0.0	0.0	0.0	1.409007059247889E-4	0.0
24-25	0.0	0.0	0.0	2.205402353605392E-4	0.0
26-27	0.0	0.0	0.0	3.920715295298474E-4	0.0
28-29	0.0	0.0	0.0	0.001096575059153792	0.0
30-31	0.0	0.0	0.0	0.003369364706897126	0.0
32-33	0.0	0.0	0.0	0.00815998870833995	0.0
34-35	0.0	0.0	0.0	0.013618359533513294	0.0
36-37	0.0	0.0	0.0	0.022507356242072805	0.0
38-39	0.0	0.0	0.0	0.04491056848411424	0.0
40-41	0.0	0.0	0.0	0.0679876536675351	0.0
42-43	0.0	0.0	0.0	0.08293538073086054	0.0
44-45	0.0	0.0	0.0	0.09964130355942138	0.0
46-47	0.0	0.0	0.0	0.11637785697622674	0.0
48-49	0.0	0.0	0.0	0.13523404709955283	0.0
50-51	0.0	0.0	0.0	0.15120483581024524	0.0
52-53	0.0	0.0	0.0	0.20258458453160194	0.0
54-55	0.0	0.0	0.0	0.26676179302151887	0.0
56-57	0.0	0.0	0.0	0.3218478429204624	0.0
58-59	0.0	0.0	0.0	0.3417944819852934	0.0
60-61	0.0	0.0	0.0	0.35707301940165964	0.0
62-63	0.0	0.0	0.0	0.36912921893470246	0.0
64-65	0.0	0.0	0.0	0.37355840199485996	0.0
66-67	0.0	0.0	0.0	0.3778773149373371	0.0
68-69	0.0	0.0	0.0	0.3822574890563034	0.0
70-71	0.0	0.0	0.0	0.38607406035157055	0.0
72-73	0.0	0.0	0.0	0.38948630788201	0.0
74-75	0.0	0.0	0.0	0.3917652236474022	0.0
76-77	0.0	0.0	0.0	0.3942279229422616	0.0
78-79	0.0	0.0	0.0	0.39575945235448756	0.0
80-81	0.0	0.0	0.0	0.3963598118840801	0.0
82-83	0.0	0.0	0.0	0.3967396311783121	0.0
84-85	0.0	0.0	0.0	0.3969785497666194	0.0
86-87	0.0	0.0	0.0	0.3971010721195975	0.0
88	0.0	0.0	0.0	0.3971194504725442	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14555	0.0	48.60422	1
GTATCAA	25205	0.0	36.22131	1
ATCAACG	31065	0.0	28.845264	3
TCAACGC	31285	0.0	28.586924	4
TATCAAC	32025	0.0	28.210434	2
CAACGCA	31800	0.0	28.137564	5
AACGCAG	31930	0.0	28.042227	6
ACGCAGA	35900	0.0	24.953232	7
CGCAGAG	36375	0.0	24.615484	8
TACATGG	26000	0.0	22.066761	2
GCAGAGT	40810	0.0	21.791952	9
ACATGGG	26100	0.0	21.38528	3
GTACATG	29355	0.0	20.458595	1
GAGTACT	27630	0.0	20.37527	12-13
GTGGTAT	7270	0.0	20.354486	1
CATGGGG	17925	0.0	19.81742	4
TGGTATC	6970	0.0	19.554478	2
CAGAGTA	40855	0.0	19.416376	10-11
AGAGTAC	38645	0.0	18.91417	10-11
GTACTAG	2580	0.0	18.615372	1
>>END_MODULE