FastQCFastQC Report
Fri 27 May 2016
SRR2138911_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138911_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6766726
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150000.049.6612551
GTATCAA277550.038.516341
ATCAACG346700.030.2263
CGAGACC31500.030.10204986-87
CAACGCA350300.029.8906085
AACGCAG351750.029.821786
TCAACGC352550.029.7486114
TATCAAC362150.029.2338732
ACCGAGG34250.028.2643390-91
GAGACCG33450.028.06687288-89
GACCGAG34300.027.71612790-91
ACGCAGA395550.026.5086887
AGACCGA35800.026.3037288-89
CGCAGAG399350.026.2564458
GCCCACG35250.025.3709982-83
CACGAGA36950.025.10279784-85
CCCACGA38150.024.07980382-83
ATCTCCG35650.023.98737174-75
GCAGAGT451500.023.1570729
TACATGG286350.023.1321452
Produced by FastQC (version 0.11.5)