##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138911_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6766726
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67571599618486	33.0	33.0	33.0	33.0	33.0
2	32.67491058452788	33.0	33.0	33.0	33.0	33.0
3	32.679758867139	33.0	33.0	33.0	33.0	33.0
4	36.60901431504689	37.0	37.0	37.0	37.0	37.0
5	36.62628706999515	37.0	37.0	37.0	37.0	37.0
6	36.5750745633856	37.0	37.0	37.0	37.0	37.0
7	36.5501130088613	37.0	37.0	37.0	37.0	37.0
8	36.54940483773098	37.0	37.0	37.0	37.0	37.0
9	36.58544486654255	37.0	37.0	37.0	37.0	37.0
10-11	36.56478472750337	37.0	37.0	37.0	37.0	37.0
12-13	36.554220977766796	37.0	37.0	37.0	37.0	37.0
14-15	38.61952678444494	40.0	37.0	40.0	37.0	40.0
16-17	38.55790747253546	40.0	37.0	40.0	37.0	40.0
18-19	38.60409805273628	40.0	37.0	40.0	37.0	40.0
20-21	38.62422735733778	40.0	37.0	40.0	37.0	40.0
22-23	38.61854726028017	40.0	37.0	40.0	37.0	40.0
24-25	38.61592279138043	40.0	37.0	40.0	37.0	40.0
26-27	38.52319133450463	40.0	37.0	40.0	37.0	40.0
28-29	38.400906376647015	40.0	37.0	40.0	37.0	40.0
30-31	38.242007298042466	40.0	37.0	40.0	37.0	40.0
32-33	38.11774404464781	40.0	37.0	40.0	37.0	40.0
34-35	38.08970665187153	40.0	37.0	40.0	37.0	40.0
36-37	38.06974056948481	40.0	37.0	40.0	37.0	40.0
38-39	38.02427835687813	40.0	37.0	40.0	33.0	40.0
40-41	37.994257129228984	40.0	37.0	40.0	33.0	40.0
42-43	37.930747358997834	40.0	37.0	40.0	33.0	40.0
44-45	37.82829244862059	40.0	37.0	40.0	33.0	40.0
46-47	37.715373194246965	40.0	37.0	40.0	33.0	40.0
48-49	37.64971278826404	40.0	37.0	40.0	33.0	40.0
50-51	37.631334963477244	40.0	37.0	40.0	33.0	40.0
52-53	37.572205002237226	40.0	37.0	40.0	33.0	40.0
54-55	37.4541500130179	40.0	37.0	40.0	33.0	40.0
56-57	37.27027185188158	38.5	37.0	40.0	33.0	40.0
58-59	37.08083532304364	37.0	37.0	40.0	33.0	40.0
60-61	36.90548059415541	37.0	37.0	40.0	33.0	40.0
62-63	36.760971452861384	37.0	37.0	40.0	33.0	40.0
64-65	36.59370710399606	37.0	37.0	40.0	33.0	40.0
66-67	36.382783144460504	37.0	37.0	40.0	33.0	40.0
68-69	36.1834190891526	37.0	37.0	40.0	33.0	40.0
70-71	35.96287315215214	37.0	37.0	37.0	33.0	40.0
72-73	35.67244313516685	37.0	33.0	37.0	33.0	40.0
74-75	35.31550884928859	37.0	33.0	37.0	33.0	40.0
76-77	33.185394838659285	35.0	33.0	37.0	27.0	37.0
78-79	34.876893225174314	37.0	33.0	37.0	33.0	37.0
80-81	35.10869444400557	37.0	33.0	37.0	33.0	37.0
82-83	34.921755150749455	37.0	33.0	37.0	33.0	37.0
84-85	34.773661995751375	37.0	33.0	37.0	33.0	37.0
86-87	34.682740450061786	37.0	33.0	37.0	33.0	37.0
88-89	34.57577664924579	37.0	33.0	37.0	33.0	37.0
90-91	34.50753632204098	37.0	33.0	37.0	33.0	37.0
92-93	34.47074312591394	37.0	33.0	37.0	33.0	37.0
94-95	34.427739612641396	37.0	33.0	37.0	33.0	37.0
96-97	34.44394380825916	37.0	33.0	37.0	33.0	37.0
98-99	34.482467023801654	37.0	33.0	37.0	33.0	37.0
100	34.43767406216706	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	2.0
18	3.0
19	10.0
20	39.0
21	188.0
22	758.0
23	2292.0
24	5282.0
25	10890.0
26	20089.0
27	32335.0
28	48717.0
29	69704.0
30	95246.0
31	128763.0
32	171676.0
33	232157.0
34	324985.0
35	487223.0
36	896594.0
37	2128969.0
38	2053695.0
39	57107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.09976022374191	20.757707753750893	14.541569222925418	22.60096279958178
2	14.696395016407784	21.175153566670435	42.273385250062624	21.855066166859157
3	17.684110438849704	27.283823031950664	30.87567035871035	24.156396170489273
4	11.836597467755821	18.154306551544504	39.84963183814918	30.159464142550497
5	12.608388843938537	38.62440102117346	35.63468295224056	13.132527182647442
6	28.78166321226295	38.90118188636305	18.920689107359912	13.39646579401409
7	25.905397367264076	33.57950855103466	23.588190742198034	16.92690333950323
8	24.624290184690526	36.243190021760896	21.28061950204863	17.851900291499952
9	26.163864178924932	16.240897001001667	21.788114370228676	35.80712444984472
10-11	23.985075192628393	27.077356010197256	29.071756399573474	19.865812397600877
12-13	24.99480322263009	24.95438179040342	29.223099333637542	20.827715653328948
14-15	22.221529289053525	25.992917402005045	27.63238824802423	24.1531650609172
16-17	20.753773686122358	28.897401786329162	28.445883577966658	21.90294094958182
18-19	20.558435497462142	27.681547915491183	31.15992135635461	20.600095230692066
20-21	21.62785164254167	26.962164311065035	31.099476168976704	20.310507877416594
22-23	21.367679214958034	26.93460338631527	30.845764415872885	20.85195298285381
24-25	21.024236504966524	27.08454913291422	31.12615706853033	20.765057293588924
26-27	20.913931960887474	27.310413061072374	30.88870558718147	20.886949390858682
28-29	20.7206307992617	27.47830643467869	31.007085452351845	20.79397731370777
30-31	21.12228753616952	27.329945286541417	30.752392667273327	20.795374510015733
32-33	20.81537048420414	27.56761484572634	30.63099913003171	20.98601554003781
34-35	21.150213278686035	27.632764381759767	30.596619100373918	20.62040323918028
36-37	21.23649689528742	27.464708857266107	30.361654083518967	20.93714016392751
38-39	20.959638218901677	27.81310655735738	30.38584695500709	20.84140826873385
40-41	21.222602165824078	27.60300221526978	30.36409441117533	20.810301207730813
42-43	21.272156827046874	27.695089669924585	29.767817115859934	21.264936387168607
44-45	21.26497333274982	28.178357571386982	29.347591385041603	21.209077710821596
46-47	21.467792730092665	28.110317046132486	28.863776555172905	21.558113668601937
48-49	21.531391320599745	28.226187656551417	28.652184580675925	21.590236442172912
50-51	21.598036410643147	28.1848113927503	28.463879865237708	21.753272331368848
52-53	21.71978513323268	28.213398488655372	28.256738207838083	21.810078170273865
54-55	21.876600598070798	28.115544961242506	28.223004413282727	21.78485002740397
56-57	21.846319857084442	28.27736163565954	28.062014521937083	21.814303985318933
58-59	21.6836718454587	28.0956417173915	28.343621530160533	21.87706490698927
60-61	21.9315155949235	28.258716194826178	28.205785960733788	21.603982249516534
62-63	21.68616848131896	28.1515958358661	28.299290367626924	21.86294531518802
64-65	21.761791493549627	28.04561088762183	28.18201828324034	22.010579335588204
66-67	21.758907958757575	28.234234837943344	28.057713179364075	21.94914402393501
68-69	21.79145397046739	28.271371299086134	28.055296861641644	21.881877868804832
70-71	21.954673580061538	27.8488259071317	27.90989348366727	22.28660702913949
72-73	21.918352390457756	27.99507561749256	27.8844344531727	22.202137538876983
74-75	21.7804629532196	28.158333525824137	27.930355971107556	22.130847549848703
76-77	21.89657206698116	27.829785394926493	28.022033162113164	22.251609375979186
78-79	21.872952914691517	27.769265792923477	27.849099664434075	22.508681627950935
80-81	21.90614868982812	28.03781154264661	27.850347017351705	22.205692750173558
82-83	22.434757021015248	27.638803090856552	27.92446249363908	22.001977394489128
84-85	22.21545659608594	27.716583086407077	27.659853748492097	22.40810656901489
86-87	21.99331824561915	27.938380007797793	27.844973324228267	22.223328422354786
88-89	22.432769962881853	27.674915619021018	27.777394507272913	22.114919910824213
90-91	22.335727559991888	27.72803065909557	27.8202470568426	22.115994724069935
92-93	22.36248768118711	27.523045515817714	27.80092798363904	22.31353881935614
94-95	22.2411552050316	27.573994209711607	27.98568400773536	22.19916657752143
96-97	22.535847974596674	27.53242057017713	28.007235387506356	21.92449606771984
98-99	23.265854018784157	29.478450784990052	28.32031894836529	18.9353762478605
100	22.31116263612123	30.214730429018648	22.186364421727244	25.287742513132876
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1961.0
1	2046.0
2	3885.0
3	5925.5
4	8242.5
5	14421.0
6	19424.5
7	22337.5
8	23771.5
9	22054.0
10	18465.5
11	15179.5
12	16260.5
13	22521.5
14	36808.0
15	56393.5
16	71618.5
17	79036.0
18	78898.0
19	72424.5
20	62193.0
21	51006.5
22	42361.5
23	39137.0
24	41155.0
25	47200.0
26	56949.0
27	67512.0
28	76528.0
29	89386.5
30	104253.0
31	118384.5
32	138578.5
33	162018.0
34	183710.5
35	202808.5
36	223511.0
37	242344.0
38	249975.0
39	251012.0
40	252018.0
41	249688.0
42	244312.5
43	248668.0
44	256886.5
45	263491.5
46	266589.5
47	265906.5
48	265792.5
49	259313.5
50	247178.5
51	235916.5
52	224505.0
53	210555.0
54	197888.5
55	181733.0
56	161668.5
57	139431.5
58	119347.0
59	102664.5
60	83036.5
61	64931.0
62	50712.0
63	37574.5
64	26621.0
65	19415.5
66	14122.0
67	10765.0
68	8506.0
69	6594.5
70	4837.5
71	2936.5
72	2090.5
73	1570.0
74	1085.0
75	779.0
76	505.5
77	306.5
78	182.0
79	118.5
80	92.0
81	74.0
82	60.0
83	40.5
84	28.0
85	19.5
86	13.0
87	9.0
88	6.5
89	4.5
90	2.5
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.4778195540945503E-5
2	0.001788161660454406
3	1.9211654203229154E-4
4	1.4778195540945503E-5
5	1.4778195540945503E-5
6	1.4778195540945503E-5
7	1.4778195540945503E-5
8	1.4778195540945503E-5
9	0.0
10-11	8.128007547520026E-5
12-13	0.007588603410275516
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.478043006026129E-5
24-25	2.734964091399839E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	6.698873859758629E-4
36-37	0.025007963356689773
38-39	0.12170300333537519
40-41	0.14419160275796042
42-43	0.030989140875954176
44-45	0.00210821907090405
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.007026048651877924
64-65	0.07323827113495116
66-67	0.18087487368884161
68-69	0.18800547753498995
70-71	0.07025206171798527
72-73	0.0024970031729715575
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	132.0
20-21	599.0
22-23	1352.0
24-25	2091.0
26-27	3552.0
28-29	7216.0
30-31	13928.0
32-33	15716.0
34-35	19403.0
36-37	20507.0
38-39	23582.0
40-41	32814.0
42-43	43648.0
44-45	51457.0
46-47	52071.0
48-49	41632.0
50-51	34756.0
52-53	38703.0
54-55	39655.0
56-57	36845.0
58-59	28518.0
60-61	49514.0
62-63	40097.0
64-65	52385.0
66-67	65607.0
68-69	62945.0
70-71	65218.0
72-73	63313.0
74-75	60800.0
76-77	56469.0
78-79	63062.0
80-81	69526.0
82-83	64305.0
84-85	72360.0
86-87	75653.0
88-89	80903.0
90-91	86433.0
92-93	87102.0
94-95	99267.0
96-97	238851.0
98-99	417657.0
100-101	4387082.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.08015349527584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.54033981156888	65.93462522713784
2	8.888429205119087	13.702429749249436
3	2.778095132649648	6.424079977473409
4	1.171573412051546	3.6122023372766883
5	0.598701098904387	2.3073986300670235
6	0.3344578763307227	1.5468038667165653
7	0.18263583303830053	0.9854318631030842
8	0.13334397127886427	0.8222539019075609
9	0.08670836284399021	0.6015144525604954
>10	0.2792875708937421	3.219524085451495
>50	0.004400211882356493	0.22887489368103942
>100	0.0014136345333095313	0.18029360419301096
>500	3.6832734426866864E-4	0.18977624527410328
>1k	2.4555156087454884E-4	0.24479116590824468
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.9556391081891006E-5	0.0	0.0	0.0	0.0
3	2.9556391081891006E-5	0.0	0.0	0.0	0.0
4	2.9556391081891006E-5	2.9556391081891006E-5	0.0	1.4778195540945503E-5	0.0
5	5.911278216378201E-5	2.9556391081891006E-5	0.0	1.4778195540945503E-5	0.0
6	7.389097770472752E-5	2.9556391081891006E-5	0.0	1.4778195540945503E-5	0.0
7	1.0344736878661853E-4	2.9556391081891006E-5	0.0	1.4778195540945503E-5	0.0
8	1.1822556432756402E-4	2.9556391081891006E-5	0.0	1.4778195540945503E-5	0.0
9	1.1822556432756402E-4	2.9556391081891006E-5	0.0	2.9556391081891006E-5	0.0
10-11	1.4778195540945504E-4	2.9556391081891006E-5	0.0	2.9556391081891006E-5	0.0
12-13	1.4778195540945504E-4	2.9556391081891006E-5	0.0	2.9556391081891006E-5	0.0
14-15	2.5122932419607353E-4	2.9556391081891006E-5	0.0	2.9556391081891006E-5	0.0
16-17	2.881748130484373E-4	2.9556391081891006E-5	0.0	2.9556391081891006E-5	0.0
18-19	4.5073496399883784E-4	4.433458662283651E-5	0.0	6.650187993425477E-5	0.0
20-21	5.320150394740381E-4	4.433458662283651E-5	0.0	1.4039285763898227E-4	0.0
22-23	5.837387238673475E-4	4.433458662283651E-5	0.0	5.467932350149836E-4	0.0
24-25	8.423571458338936E-4	7.389097770472752E-5	0.0	0.0016847142916677875	0.0
26-27	8.423571458338936E-4	7.389097770472752E-5	0.0	0.003694548885236376	0.0
28-29	0.0010861973722594944	7.389097770472752E-5	0.0	0.013706776364226955	0.0
30-31	0.0011970338388165858	7.389097770472752E-5	0.0	0.01877569743477126	0.0
32-33	0.0011970338388165858	7.389097770472752E-5	0.0	0.02019440420670203	0.0
34-35	0.0013004812076032042	8.866917324567302E-5	0.0	0.021709169249648944	0.0
36-37	0.001455652260783132	8.866917324567302E-5	0.0	0.02430274256708488	0.0
38-39	0.001699492487208733	8.866917324567302E-5	0.0	0.029319939953235875	0.0
40-41	0.0017290488782906238	8.866917324567302E-5	0.0	0.03463270125020579	0.0
42-43	0.0019137763225524425	1.0344736878661851E-4	0.0	0.03912527269465322	0.0
44-45	0.002083725571273316	1.1822556432756402E-4	0.0	0.04401685541870618	0.0
46-47	0.0022832312110760804	1.1822556432756402E-4	0.0	0.04941828588892176	0.0
48-49	0.002467958655337899	1.3300375986850953E-4	0.0	0.056556154335198436	0.0
50-51	0.002467958655337899	1.6256015095040052E-4	0.0	0.06327284420855817	0.0
52-53	0.002467958655337899	1.7733834649134603E-4	0.0	0.08437610744102836	0.0
54-55	0.002527071437501681	1.7733834649134603E-4	0.0	0.11344481807006815	0.0
56-57	0.002645297001829245	2.142838353437098E-4	0.0	0.13910715462692003	0.0
58-59	0.0026822424906816087	2.2167293311418254E-4	0.0	0.27064787313687594	0.0
60-61	0.0027191879795339727	2.2167293311418254E-4	0.0	0.39196946943026806	0.0
62-63	0.0027930789572387003	2.2906203088465528E-4	0.0	0.3966246010256659	0.0
64-65	0.0028374135438615365	2.3645112865512804E-4	0.0	0.40035609539975464	0.0
66-67	0.0030147518903528825	2.3645112865512804E-4	0.0	0.40566885669672453	0.0
68-69	0.003044308281434774	2.3645112865512804E-4	0.0	0.41191264431277397	0.0
70-71	0.003155144747991865	2.66007519737019E-4	0.0	0.4170997909476459	0.0
72-73	0.003229035725696592	3.029530085893828E-4	0.0	0.4212450747968811	0.0
74-75	0.003265981214548956	3.2512030190080105E-4	0.0	0.4244962778158891	0.0
76-77	0.0032807594100899016	3.398984974417466E-4	0.0	0.42764403346611046	0.0
78-79	0.00330292670340132	3.6206579075316484E-4	0.0	0.4299642101660389	0.0
80-81	0.0037462725696296847	3.9901127960552857E-4	0.0	0.4307105090408567	0.0
82-83	0.0038275526451048854	3.9901127960552857E-4	0.0	0.43128685866695354	0.0
84-85	0.003901443622809613	4.0640037737600134E-4	0.0	0.43148636430675635	0.0
86-87	0.00397533460051434	4.285676706874196E-4	0.0	0.4316563135554772	0.0
88	0.003990112796055286	4.4334586622836507E-4	0.0	0.43167109175101814	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15000	0.0	49.661255	1
GTATCAA	27755	0.0	38.51634	1
ATCAACG	34670	0.0	30.226	3
CGAGACC	3150	0.0	30.102049	86-87
CAACGCA	35030	0.0	29.890608	5
AACGCAG	35175	0.0	29.82178	6
TCAACGC	35255	0.0	29.748611	4
TATCAAC	36215	0.0	29.233873	2
ACCGAGG	3425	0.0	28.26433	90-91
GAGACCG	3345	0.0	28.066872	88-89
GACCGAG	3430	0.0	27.716127	90-91
ACGCAGA	39555	0.0	26.508688	7
AGACCGA	3580	0.0	26.30372	88-89
CGCAGAG	39935	0.0	26.256445	8
GCCCACG	3525	0.0	25.37099	82-83
CACGAGA	3695	0.0	25.102797	84-85
CCCACGA	3815	0.0	24.079803	82-83
ATCTCCG	3565	0.0	23.987371	74-75
GCAGAGT	45150	0.0	23.157072	9
TACATGG	28635	0.0	23.132145	2
>>END_MODULE