FastQCFastQC Report
Fri 27 May 2016
SRR2138910_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138910_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8180027
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA180150.043.1174741
ATCTCGT46750.039.8647794
TATCTCG59500.035.67264694
GTATCAA316400.035.299991
ATCAACG377950.029.0582683
CAACGCA383850.028.566535
TATCAAC391600.028.4217852
AACGCAG387300.028.41546
TCAACGC386900.028.3972574
CGCTATC92350.026.06660594
ACGCAGA428050.025.6091777
CGCAGAG428200.025.5395668
TACATGG323200.023.1363832
GTACATG338750.022.6108571
GCAGAGT485300.022.472189
ACATGGG324500.022.3763713
TATGCCG23000.021.83732886-87
GTACTAG26100.021.7197021
CTCGTAT23650.021.19057882-83
TGCCGTC25000.020.72589388-89
Produced by FastQC (version 0.11.5)