##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138910_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8180027
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.7167557026401	33.0	33.0	33.0	33.0	33.0
2	32.71402331557096	33.0	33.0	33.0	33.0	33.0
3	32.72303722224878	33.0	33.0	33.0	33.0	33.0
4	36.65637717332718	37.0	37.0	37.0	37.0	37.0
5	36.6698864196903	37.0	37.0	37.0	37.0	37.0
6	36.61751813288636	37.0	37.0	37.0	37.0	37.0
7	36.59435622889753	37.0	37.0	37.0	37.0	37.0
8	36.59327163590047	37.0	37.0	37.0	37.0	37.0
9	36.628744257201106	37.0	37.0	37.0	37.0	37.0
10-11	36.60976094577683	37.0	37.0	37.0	37.0	37.0
12-13	36.599430784274915	37.0	37.0	37.0	37.0	37.0
14-15	38.73550013954721	40.0	37.0	40.0	37.0	40.0
16-17	38.67736872995651	40.0	37.0	40.0	37.0	40.0
18-19	38.708439898792506	40.0	37.0	40.0	37.0	40.0
20-21	38.72589317966408	40.0	37.0	40.0	37.0	40.0
22-23	38.71996154943005	40.0	37.0	40.0	37.0	40.0
24-25	38.71691130136368	40.0	37.0	40.0	37.0	40.0
26-27	38.632578941072346	40.0	37.0	40.0	37.0	40.0
28-29	38.51916468835782	40.0	37.0	40.0	37.0	40.0
30-31	38.37805905661325	40.0	37.0	40.0	37.0	40.0
32-33	38.266834689305725	40.0	37.0	40.0	37.0	40.0
34-35	38.24103915324277	40.0	37.0	40.0	37.0	40.0
36-37	38.223636761203835	40.0	37.0	40.0	37.0	40.0
38-39	38.17606467438672	40.0	37.0	40.0	37.0	40.0
40-41	38.14551491838186	40.0	37.0	40.0	37.0	40.0
42-43	38.08438422950837	40.0	37.0	40.0	37.0	40.0
44-45	37.98303988050522	40.0	37.0	40.0	33.0	40.0
46-47	37.8738891669504	40.0	37.0	40.0	33.0	40.0
48-49	37.804710905557556	40.0	37.0	40.0	33.0	40.0
50-51	37.772537186709954	40.0	37.0	40.0	33.0	40.0
52-53	37.70877473091268	40.0	37.0	40.0	33.0	40.0
54-55	37.59289961960187	40.0	37.0	40.0	33.0	40.0
56-57	37.41305171554184	40.0	37.0	40.0	33.0	40.0
58-59	37.2298592649632	37.0	37.0	40.0	33.0	40.0
60-61	37.051606545282425	37.0	37.0	40.0	33.0	40.0
62-63	36.90450634675426	37.0	37.0	40.0	33.0	40.0
64-65	36.72960285875389	37.0	37.0	40.0	33.0	40.0
66-67	36.51730246598936	37.0	37.0	40.0	33.0	40.0
68-69	36.31122305456519	37.0	37.0	40.0	33.0	40.0
70-71	36.088807114250486	37.0	37.0	38.5	33.0	40.0
72-73	35.80581497542401	37.0	35.0	37.0	33.0	40.0
74-75	35.44432450364425	37.0	33.0	37.0	33.0	40.0
76-77	33.31150705466073	35.0	33.0	37.0	27.0	37.0
78-79	34.97541006004164	37.0	33.0	37.0	33.0	37.0
80-81	35.19256513284719	37.0	33.0	37.0	33.0	37.0
82-83	35.00469198127042	37.0	33.0	37.0	33.0	37.0
84-85	34.86644481607442	37.0	33.0	37.0	33.0	37.0
86-87	34.775585672247445	37.0	33.0	37.0	33.0	37.0
88-89	34.67592892325297	37.0	33.0	37.0	33.0	37.0
90-91	34.61650754835926	37.0	33.0	37.0	33.0	37.0
92-93	34.58541112761514	37.0	33.0	37.0	33.0	37.0
94-95	34.53640960464378	37.0	33.0	37.0	33.0	37.0
96-97	34.55005127860592	37.0	33.0	37.0	33.0	37.0
98-99	34.584132656030434	37.0	33.0	37.0	33.0	37.0
100	34.531492879749	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	3.0
19	9.0
20	41.0
21	201.0
22	797.0
23	2469.0
24	5868.0
25	11883.0
26	21548.0
27	35809.0
28	53351.0
29	76766.0
30	105427.0
31	142500.0
32	191403.0
33	259507.0
34	366261.0
35	556378.0
36	1038413.0
37	2575205.0
38	2666752.0
39	69432.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.35764560621759	20.250121484958786	14.309785116793652	23.082447792029974
2	14.755470783672243	20.64961739569902	42.334435756304416	22.26047606432432
3	18.257867127203493	26.480574189375357	30.30567532911598	24.95588335430517
4	12.10253024899068	17.745923759401713	39.32962307572409	30.82192291588351
5	13.07387202361851	38.12290304736232	35.35464744912149	13.448577479897677
6	29.71712189143676	37.9002875908081	18.79838998047072	13.584200537284422
7	26.52425230381269	32.97894982498224	23.068994760041743	17.427803111163325
8	24.82351219623114	36.167460082955714	21.013341398506384	17.99568632230676
9	26.140647213022643	16.33279450055605	21.431848574582947	36.094709711838355
10-11	24.398889000271392	26.715558152669555	28.652179334524853	20.2333735125342
12-13	25.306491941121028	24.383973433931704	28.955059544604218	21.354475080343054
14-15	22.399412618075708	25.53608319385743	27.23052136624977	24.83398282181709
16-17	21.28391507754192	28.38593931291425	28.010934438235964	22.31921117130787
18-19	21.026422039927255	27.491113415640317	30.36558436787556	21.116880176556872
20-21	22.12980857158785	26.665896881182938	30.216401003060234	20.98789354416898
22-23	21.83268923096335	26.641134467875382	30.02474583086944	21.50143047029183
24-25	21.52426670677718	26.812929403359192	30.28889657266071	21.373907317202914
26-27	21.440519487700357	27.06044122398106	30.065731101773054	21.433308186545535
28-29	21.215265856236464	27.34337160262848	30.106013230539304	21.335349310595753
30-31	21.663787849056888	27.02188166938166	29.85850875981322	21.455821721748233
32-33	21.416426158561244	27.240422434485527	29.80081660677378	21.54233480017945
34-35	21.65900464671586	27.35147786351063	29.77813514167841	21.2113823480951
36-37	21.661299606175266	27.22354334744536	29.6432282196879	21.471928826691475
38-39	21.52167769609562	27.347855997412555	29.703366465265457	21.42709984122636
40-41	21.76191836872654	27.15489517113851	29.661004476727516	21.422181983407434
42-43	21.778956549154106	27.259767093412407	29.148824482676165	21.812451874757315
44-45	21.73770543565779	27.741658641322363	28.751033295969435	21.76960262705041
46-47	21.93368965766306	27.618565304653984	28.384636476062415	22.06310856162054
48-49	22.026621836496176	27.75122727993558	28.18754203310791	22.034608850460334
50-51	21.98732092883952	27.792143480746073	27.971016192568225	22.24951939784618
52-53	21.977536328910002	27.782241332522716	27.798492212290988	22.441730126276294
54-55	22.19042702889072	27.623481472646468	27.903498600989767	22.282592897473048
56-57	22.13356475794705	27.489476448624174	27.87697463354304	22.499984159885734
58-59	21.97796939850236	27.274024465253355	28.179720716710293	22.56828541953399
60-61	22.201318550542243	27.502014159674154	28.223919265108172	22.07274802467543
62-63	21.837924963210273	27.53694305291588	28.17809046145706	22.44704152241679
64-65	21.74368600814888	27.829682715502024	27.898258361704354	22.52837291464474
66-67	21.787831534934845	28.169048850843904	27.535207045300513	22.507912568920737
68-69	21.831998404483784	28.076831184990713	27.48512882227896	22.606041588246544
70-71	21.960262357566044	27.51103764309959	27.410602793689076	23.118097205645295
72-73	22.10290029142214	27.360483345901716	27.463068204634116	23.073548158042023
74-75	22.205083295324464	27.37913178793733	27.540250277241178	22.87553463949703
76-77	22.439663210028186	27.25987416127135	27.119582603536664	23.1808800251638
78-79	22.404763010380826	27.204701740468018	26.9047522369456	23.485783012205555
80-81	22.369012426583062	27.394589685295017	27.054940228802344	23.18145765931957
82-83	22.66940477049111	27.20153442062537	27.09206342532874	23.036997383554777
84-85	22.71570351314553	27.497699485148843	26.817395750565254	22.969201251140372
86-87	22.77411476109452	27.53282996752715	26.95202725553964	22.741028015838687
88-89	22.89662746478762	27.386460840244624	27.0734386132673	22.643473081700453
90-91	22.789007848485156	27.089653566042315	27.12416643626412	22.99717214920841
92-93	22.859650754618	26.8421516195793	27.235845909014557	23.062351716788147
94-95	22.19359250884457	27.070308823490265	27.77027908450273	22.96581958316244
96-97	22.319929289982856	27.060347939725922	27.808787184104844	22.81093558618638
98-99	23.496457659956736	28.898297005217195	28.152467105849215	19.452778228976854
100	22.597874303543165	29.779227869063828	21.807253484227363	25.815644343165644
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1734.0
1	1805.0
2	3458.0
3	5356.0
4	7722.5
5	13793.5
6	18669.0
7	21223.5
8	22221.5
9	20607.5
10	17777.5
11	15269.0
12	16483.0
13	22477.0
14	36480.0
15	55517.0
16	70223.5
17	77949.0
18	78234.5
19	71954.5
20	62320.5
21	52151.0
22	44460.5
23	42018.0
24	44799.5
25	51542.5
26	62038.5
27	73268.5
28	83598.5
29	98306.0
30	113417.5
31	127737.5
32	147822.0
33	167156.5
34	186510.5
35	210211.5
36	234127.0
37	257755.0
38	270551.0
39	274023.0
40	280597.5
41	285581.0
42	290989.0
43	303222.0
44	317882.0
45	333662.5
46	343489.0
47	350505.5
48	357050.5
49	352817.5
50	339943.5
51	327384.0
52	314950.0
53	296347.5
54	275130.0
55	249316.5
56	219566.5
57	188943.5
58	163011.5
59	139186.5
60	110157.5
61	84646.0
62	64751.0
63	47420.5
64	33122.0
65	23115.0
66	16877.5
67	13154.5
68	10337.5
69	7946.0
70	6057.0
71	3781.0
72	2570.5
73	1850.5
74	1258.5
75	822.0
76	501.5
77	329.5
78	209.0
79	145.0
80	124.5
81	105.5
82	74.5
83	49.0
84	32.5
85	20.5
86	16.5
87	16.0
88	12.5
89	7.5
90	4.5
91	2.5
92	1.5
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.4449797048347153E-5
2	0.0020293331550128137
3	9.779918819338861E-5
4	2.4449797048347153E-5
5	2.4449797048347153E-5
6	2.4449797048347153E-5
7	0.0
8	0.0
9	0.0
10-11	8.557428966921504E-5
12-13	0.006974304608041025
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.113251156565986E-6
24-25	2.506900702763754E-4
26-27	0.0
28-29	2.448059221490239E-5
30-31	2.4512738841402947E-5
32-33	2.4565137194141782E-5
34-35	5.479878164452621E-4
36-37	0.02525994453561115
38-39	0.1222041016126764
40-41	0.1441539797900467
42-43	0.032181255659211025
44-45	0.002292301535785195
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.006696127417360008
64-65	0.07374743064365687
66-67	0.17927172150670917
68-69	0.18731222917179052
70-71	0.07053458509021206
72-73	0.0024300742668081693
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	144.0
20-21	620.0
22-23	1338.0
24-25	2399.0
26-27	4034.0
28-29	8421.0
30-31	16768.0
32-33	19853.0
34-35	25464.0
36-37	28894.0
38-39	31261.0
40-41	47165.0
42-43	58783.0
44-45	73177.0
46-47	70480.0
48-49	61975.0
50-51	36024.0
52-53	17439.0
54-55	17009.0
56-57	16013.0
58-59	32870.0
60-61	54271.0
62-63	55123.0
64-65	54888.0
66-67	69468.0
68-69	66595.0
70-71	71340.0
72-73	66287.0
74-75	64153.0
76-77	60143.0
78-79	65289.0
80-81	68151.0
82-83	66451.0
84-85	76033.0
86-87	81377.0
88-89	89081.0
90-91	94664.0
92-93	96047.0
94-95	106703.0
96-97	284514.0
98-99	551463.0
100-101	5467855.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.46077599854047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.44693938000697	63.724315763170324
2	9.314793945117218	14.05803158730103
3	3.0260069740801	6.850345033232391
4	1.3274769482060054	4.0068976252719795
5	0.6824207922572323	2.574800127063477
6	0.3792288095336908	1.7170140149049007
7	0.2428944278460899	1.2830301407691211
8	0.15875572871668678	0.958386438653997
9	0.10754698203795678	0.7304020848796782
>10	0.3087910028776882	3.4407192573034338
>50	0.0036218304700563093	0.1774617146740564
>100	0.0011307813904776391	0.1774293223913488
>500	1.7836248415546973E-4	0.09459246229739743
>1k	2.1403497566782864E-4	0.20657442808690787
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	7.334939114504145E-5	0.0	0.0	0.0	0.0
3	7.334939114504145E-5	0.0	0.0	0.0	0.0
4	7.334939114504145E-5	0.0	0.0	0.0	0.0
5	1.3447388376590933E-4	0.0	0.0	0.0	0.0
6	2.3227307195929794E-4	0.0	0.0	0.0	0.0
7	5.623453321119845E-4	0.0	0.0	0.0	0.0
8	5.990200276845052E-4	0.0	0.0	0.0	0.0
9	5.990200276845052E-4	0.0	0.0	0.0	0.0
10-11	6.234698247328523E-4	0.0	0.0	0.0	0.0
12-13	6.90706766615807E-4	0.0	0.0	0.0	0.0
14-15	8.740802444784107E-4	0.0	0.0	2.4449797048347153E-5	0.0
16-17	9.107549400509314E-4	0.0	0.0	3.6674695572520726E-5	0.0
18-19	0.0012163774031552707	0.0	0.0	9.168673893130181E-5	0.0
20-21	0.0014303131273283084	0.0	0.0	1.6503613007634328E-4	0.0
22-23	0.0014853251706870895	0.0	0.0	4.4620879613233553E-4	0.0
24-25	0.0019070841697710777	0.0	0.0	0.0016625861992876061	0.0
26-27	0.0019070841697710777	0.0	0.0	0.003960867121832239	0.0
28-29	0.0024083050092621942	0.0	0.0	0.011014633570280392	0.0
30-31	0.002567228690076451	0.0	0.0	0.012200448727125227	0.0
32-33	0.0026039033856489714	0.0	0.0	0.014437605157048993	0.0
34-35	0.0029523129935879184	0.0	0.0	0.016931484455980403	0.0
36-37	0.0033312848478372994	0.0	0.0	0.02218819082137504	0.0
38-39	0.0037041442528245932	0.0	0.0	0.08343493242748465	0.0
40-41	0.0038080558902800687	0.0	0.0	0.14866699095247485	0.0
42-43	0.004174802846005276	0.0	0.0	0.16278063629863326	0.0
44-45	0.004486537758371702	0.0	0.0	0.18243216067624227	0.0
46-47	0.005427854944733067	0.0	0.0	0.2063379497402637	0.0
48-49	0.005733477407837407	0.0	0.0	0.2292840842701375	0.0
50-51	0.005733477407837407	1.2224898524173576E-5	0.0	0.2531654235371106	0.0
52-53	0.005751814755623667	1.2224898524173576E-5	0.0	0.554496947259465	0.0
54-55	0.0058557263930791425	1.2224898524173576E-5	0.0	0.9648256662233511	0.0
56-57	0.006124674160610961	1.2224898524173576E-5	0.0	1.4230454251556872	0.0
58-59	0.006136899059135135	1.2224898524173576E-5	0.0	1.941118287262377	0.0
60-61	0.006173573754707656	1.2224898524173576E-5	0.0	2.089938089446404	0.0
62-63	0.006277485392163131	1.2224898524173576E-5	0.0	2.0951458962177023	0.0
64-65	0.006344722334046086	1.2224898524173576E-5	0.0	2.0994796227445214	0.0
66-67	0.00673591908681964	1.2224898524173576E-5	0.0	2.1037033251846236	0.0
68-69	0.006845943173537202	1.2224898524173576E-5	0.0	2.108305999478975	0.0
70-71	0.007047653999186066	1.2224898524173576E-5	0.0	2.112193517209662	0.0
72-73	0.007243252375572843	1.2224898524173576E-5	0.0	2.1154636775648785	0.0
74-75	0.007377726259338753	1.2224898524173576E-5	0.0	2.1182509544283903	0.0
76-77	0.0074449632012217075	1.2224898524173576E-5	0.0	2.121117693132309	0.0
78-79	0.007457188099745881	1.2224898524173576E-5	0.0	2.1231409138380597	0.0
80-81	0.008154007315623775	1.2224898524173576E-5	0.0	2.1238560704017235	0.0
82-83	0.008343493242748466	1.2224898524173576E-5	0.0	2.1244184157338353	0.0
84-85	0.008551316517659415	1.2224898524173576E-5	0.0	2.1246506888057946	0.0
86-87	0.008759139792570366	1.2224898524173576E-5	0.0	2.12475460044325	0.0
88	0.008801926937404975	1.2224898524173576E-5	0.0	2.1247729377910365	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18015	0.0	43.117474	1
ATCTCGT	4675	0.0	39.86477	94
TATCTCG	5950	0.0	35.672646	94
GTATCAA	31640	0.0	35.29999	1
ATCAACG	37795	0.0	29.058268	3
CAACGCA	38385	0.0	28.56653	5
TATCAAC	39160	0.0	28.421785	2
AACGCAG	38730	0.0	28.4154	6
TCAACGC	38690	0.0	28.397257	4
CGCTATC	9235	0.0	26.066605	94
ACGCAGA	42805	0.0	25.609177	7
CGCAGAG	42820	0.0	25.539566	8
TACATGG	32320	0.0	23.136383	2
GTACATG	33875	0.0	22.610857	1
GCAGAGT	48530	0.0	22.47218	9
ACATGGG	32450	0.0	22.376371	3
TATGCCG	2300	0.0	21.837328	86-87
GTACTAG	2610	0.0	21.719702	1
CTCGTAT	2365	0.0	21.190578	82-83
TGCCGTC	2500	0.0	20.725893	88-89
>>END_MODULE