FastQCFastQC Report
Fri 27 May 2016
SRR2138909_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138909_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7362070
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA216700.038.0548321
GTATCAA354850.031.699591
CGCTACG3151.8189894E-1228.24382494
ATCAACG425200.025.8392663
AACGCAG435350.025.3934046
CAACGCA435900.025.1954655
TCAACGC439350.025.0637744
TATCAAC447900.024.9842992
GTACTAG24200.023.3352811
ACGCAGA478400.022.9173377
CGCAGAG480950.022.795838
GTACATG319300.021.717361
TACATGG317900.021.499522
GCAGAGT526100.020.760559
ACATGGG320750.020.712713
GAGTACT337900.019.58924312-13
CATGGGG217850.019.0029564
TAACGCC9650.018.9325434
CAGAGTA502400.018.74756410-11
AGAGTAC484450.018.7135510-11
Produced by FastQC (version 0.11.5)