##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138909_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7362070
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6875668120515	33.0	33.0	33.0	33.0	33.0
2	32.68188063954839	33.0	33.0	33.0	33.0	33.0
3	32.68414617084597	33.0	33.0	33.0	33.0	33.0
4	36.612141558012894	37.0	37.0	37.0	37.0	37.0
5	36.62642232415612	37.0	37.0	37.0	37.0	37.0
6	36.581069590482024	37.0	37.0	37.0	37.0	37.0
7	36.553029922290875	37.0	37.0	37.0	37.0	37.0
8	36.55708992172038	37.0	37.0	37.0	37.0	37.0
9	36.59185663814661	37.0	37.0	37.0	37.0	37.0
10-11	36.57603235231396	37.0	37.0	37.0	37.0	37.0
12-13	36.562526979504405	37.0	37.0	37.0	37.0	37.0
14-15	38.6172666790726	40.0	37.0	40.0	37.0	40.0
16-17	38.559171061943175	40.0	37.0	40.0	37.0	40.0
18-19	38.60137074219615	40.0	37.0	40.0	37.0	40.0
20-21	38.6249761590733	40.0	37.0	40.0	37.0	40.0
22-23	38.624324615949234	40.0	37.0	40.0	37.0	40.0
24-25	38.62015456843304	40.0	37.0	40.0	37.0	40.0
26-27	38.52331978407841	40.0	37.0	40.0	37.0	40.0
28-29	38.404743233882016	40.0	37.0	40.0	37.0	40.0
30-31	38.24892015410951	40.0	37.0	40.0	37.0	40.0
32-33	38.1274240456942	40.0	37.0	40.0	37.0	40.0
34-35	38.10701621117497	40.0	37.0	40.0	37.0	40.0
36-37	38.094695760974666	40.0	37.0	40.0	37.0	40.0
38-39	38.053479367225485	40.0	37.0	40.0	37.0	40.0
40-41	38.02900756764226	40.0	37.0	40.0	35.0	40.0
42-43	37.97030305792477	40.0	37.0	40.0	33.0	40.0
44-45	37.860169299417315	40.0	37.0	40.0	33.0	40.0
46-47	37.74430339792851	40.0	37.0	40.0	33.0	40.0
48-49	37.68057497981415	40.0	37.0	40.0	33.0	40.0
50-51	37.65653128540319	40.0	37.0	40.0	33.0	40.0
52-53	37.58994479139791	40.0	37.0	40.0	33.0	40.0
54-55	37.46633358740977	40.0	37.0	40.0	33.0	40.0
56-57	37.27218578824575	38.5	37.0	40.0	33.0	40.0
58-59	37.068935090351516	37.0	37.0	40.0	33.0	40.0
60-61	36.877685009240025	37.0	37.0	40.0	33.0	40.0
62-63	36.72084437126091	37.0	37.0	40.0	33.0	40.0
64-65	36.5313908936768	37.0	37.0	40.0	33.0	40.0
66-67	36.30950220355919	37.0	37.0	40.0	33.0	40.0
68-69	36.11200879086107	37.0	37.0	38.5	33.0	40.0
70-71	35.89740372132243	37.0	37.0	37.0	33.0	40.0
72-73	35.62756381001396	37.0	33.0	37.0	33.0	40.0
74-75	35.28111109204941	37.0	33.0	37.0	33.0	40.0
76-77	33.143396142297554	33.0	33.0	37.0	27.0	37.0
78-79	34.86201943530595	37.0	33.0	37.0	33.0	37.0
80-81	35.111060207741644	37.0	33.0	37.0	33.0	37.0
82-83	34.93477881110452	37.0	33.0	37.0	33.0	37.0
84-85	34.80750978290508	37.0	33.0	37.0	33.0	37.0
86-87	34.727313925267886	37.0	33.0	37.0	33.0	37.0
88-89	34.6357728177273	37.0	33.0	37.0	33.0	37.0
90-91	34.57645120777779	37.0	33.0	37.0	33.0	37.0
92-93	34.540274461184	37.0	33.0	37.0	33.0	37.0
94-95	34.50401054568421	37.0	33.0	37.0	33.0	37.0
96-97	34.52238311696557	37.0	33.0	37.0	33.0	37.0
98-99	34.554183238566296	37.0	33.0	37.0	33.0	37.0
100	34.51639413644043	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	5.0
18	4.0
19	12.0
20	54.0
21	194.0
22	778.0
23	2262.0
24	5335.0
25	10890.0
26	19193.0
27	31905.0
28	48193.0
29	69016.0
30	96624.0
31	132342.0
32	178294.0
33	246887.0
34	348503.0
35	530929.0
36	989344.0
37	2371668.0
38	2215645.0
39	63990.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.13420739933399	20.63357741330289	14.483471220314728	22.74874396704839
2	14.845419163234261	20.569806250824342	42.45419788069478	22.130576705246618
3	18.043504659428297	27.148372290263246	30.569424707371383	24.238698342937074
4	12.514931262538934	17.501545081750105	38.85922030081213	31.124303354898824
5	12.913052986456256	38.00539793835158	35.40653647683328	13.675012598358885
6	29.43517244470645	37.89254924226474	18.46204939643334	14.210228916595469
7	25.869707840322082	33.12304827310797	23.74386551608447	17.263378370485473
8	24.718754372071984	36.2017883557206	20.971167076650996	18.10829019555641
9	26.03433545184982	16.360045476340215	21.96507232340904	35.64054674840092
10-11	24.50761257356718	26.72343154877705	28.416482688583166	20.3524731890726
12-13	25.480285655272368	24.38900443324931	29.026762845036096	21.10394706644223
14-15	22.552685589786567	25.32835194449387	27.361516529997676	24.757445935721883
16-17	20.99779002372974	28.404497648079957	28.26497167236932	22.332740655820984
18-19	20.974685108943543	27.38820060118961	30.788969678364918	20.84814461150193
20-21	21.952563153871417	26.339169069027356	30.820175958417114	20.888091818684114
22-23	21.860555699112656	26.146787993699427	30.731976813852995	21.26067949333492
24-25	21.333737108188856	26.45915248183268	30.92573856112606	21.281371848852405
26-27	21.26115140213089	26.652997288215545	30.846770753783638	21.23908055586993
28-29	21.033401193817582	27.02677957595553	30.82707099353041	21.11274823669648
30-31	21.33681527341015	26.886019259080644	30.575628977507357	21.20153649000185
32-33	21.26964303728642	27.15990910505411	30.314018442304107	21.256429415355363
34-35	21.275169804808286	27.100657784089847	30.472660024337134	21.151512386764733
36-37	21.52945062870777	27.004030003448598	30.263527981465344	21.202991386378283
38-39	21.528126059589187	27.13125633923253	30.24179010339603	21.09882749778225
40-41	21.397374167083374	27.02047210786912	30.19028877069763	21.391864954349877
42-43	21.456781217003996	27.293412908440644	29.61320842301432	21.636597451541036
44-45	21.462686646493008	27.584439512318554	29.32034320452019	21.632530636668246
46-47	21.76352258024774	27.486693183821377	28.78592308830996	21.96386114762092
48-49	22.01543300766208	27.616242602260765	28.28265955247656	22.085664837600604
50-51	21.873688385328254	27.785878881540743	28.021650821056802	22.318781912074197
52-53	21.86649871667813	27.82810392898556	27.891829936192746	22.413567418143565
54-55	21.99180004331102	27.756179597778118	27.82683695859518	22.42518340031568
56-57	22.06581476718504	27.525830150227776	27.89981649844734	22.508538584139846
58-59	21.85802782287767	27.552512679950375	28.14867857015374	22.44078092701822
60-61	22.121938518577416	27.837454805681972	27.770951532358275	22.269655143382337
62-63	22.373340876069033	27.591231758525065	27.672705260051334	22.362722105354564
64-65	22.05684171105008	27.71096201661608	27.586617991948515	22.645578280385323
66-67	22.164625447255602	27.64403134319668	27.50420515968026	22.687138049867453
68-69	22.07146917997347	27.563124792091802	27.657048464061667	22.708357563873058
70-71	22.225936224338874	27.201951330138634	27.760473157296207	22.811639288226285
72-73	22.410673397901736	27.283846805148404	27.66974706284442	22.635732734105442
74-75	22.192929458289	27.537325035418558	27.663502213812276	22.606243292480162
76-77	22.43796010809284	27.36318496679694	27.502177490089526	22.6966774350207
78-79	22.33276911792131	27.163955483309586	27.63760978749965	22.865665611269456
80-81	22.228732309289214	27.35265298825147	27.647507570109763	22.77110713234956
82-83	22.599314313643443	27.067730949082296	27.710519115501995	22.622435621772265
84-85	22.72829124040197	27.109497928413727	27.416956482700428	22.745254348483876
86-87	22.323508348978617	27.380670844044218	27.620501708415684	22.675319098561474
88-89	22.702551280245025	26.934100012992403	27.495642245883868	22.867706460878708
90-91	22.799844052321596	26.77143913740651	27.705481404873865	22.723235405398025
92-93	22.820973137389707	26.964457126525165	27.45693563909101	22.757634096994117
94-95	22.52035335015221	26.839673346799707	27.585565576310454	23.054407726737626
96-97	22.66692102663324	26.96379368590677	27.69018327375619	22.679102013703805
98-99	23.704685901807025	29.036971530855777	27.69426568360041	19.56407688373679
100	23.08740286120445	29.071722183323896	21.550669628150626	26.290205327321033
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3250.0
1	2970.5
2	4855.0
3	7274.0
4	9891.5
5	17383.0
6	23563.0
7	26956.0
8	28262.5
9	25791.0
10	21349.0
11	17067.5
12	17815.5
13	24890.0
14	41386.0
15	63849.5
16	81469.0
17	90295.5
18	90261.5
19	82695.5
20	70692.0
21	57928.5
22	48794.0
23	46458.0
24	49657.0
25	56271.0
26	65956.0
27	76313.5
28	85116.5
29	97327.0
30	111452.5
31	124466.0
32	142125.5
33	160059.5
34	177436.0
35	196400.0
36	215258.5
37	237505.0
38	249542.0
39	250146.0
40	252431.0
41	253770.5
42	259636.5
43	276579.5
44	295558.0
45	312924.0
46	328056.5
47	342176.5
48	349536.0
49	342258.5
50	331135.5
51	319757.5
52	301493.0
53	278021.0
54	252061.5
55	226050.0
56	202100.5
57	175907.5
58	147543.5
59	123057.5
60	99254.0
61	75148.5
62	57177.0
63	43767.5
64	32117.0
65	22826.0
66	16858.5
67	12736.5
68	9453.0
69	7001.0
70	5409.0
71	3936.5
72	2737.5
73	1912.0
74	1375.0
75	907.5
76	583.0
77	403.5
78	274.5
79	184.5
80	116.5
81	75.0
82	64.5
83	51.0
84	33.0
85	21.0
86	12.5
87	7.5
88	3.5
89	2.0
90	1.0
91	1.0
92	1.5
93	1.0
94	1.5
95	1.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.7166272529329386E-5
2	0.0017522245781417456
3	1.0866509011731754E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	8.829038572032051E-5
12-13	0.007328102014786602
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.9407980700417066E-4
26-27	0.0
28-29	2.720857995360257E-5
30-31	2.0446182036720527E-5
32-33	6.839348357831424E-6
34-35	5.702265221424112E-4
36-37	0.025078840438451936
38-39	0.12380908873892305
40-41	0.14666174679444846
42-43	0.032476183009003144
44-45	0.0021834247750192647
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.00766065952458293
64-65	0.07911378582733944
66-67	0.1927001881090307
68-69	0.20063624368171973
70-71	0.07447104619914395
72-73	0.0025666771216535183
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	198.0
20-21	912.0
22-23	1565.0
24-25	2586.0
26-27	4536.0
28-29	10210.0
30-31	24407.0
32-33	30566.0
34-35	39803.0
36-37	52318.0
38-39	61927.0
40-41	67937.0
42-43	79965.0
44-45	94532.0
46-47	90860.0
48-49	76479.0
50-51	62733.0
52-53	56182.0
54-55	59633.0
56-57	59283.0
58-59	57829.0
60-61	55103.0
62-63	60540.0
64-65	63542.0
66-67	71446.0
68-69	68760.0
70-71	71745.0
72-73	67709.0
74-75	65552.0
76-77	60769.0
78-79	64825.0
80-81	65785.0
82-83	64359.0
84-85	73369.0
86-87	80777.0
88-89	86528.0
90-91	90864.0
92-93	89580.0
94-95	100619.0
96-97	233097.0
98-99	415155.0
100-101	4477485.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.60874350964342
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.04397279889707	65.05676669902545
2	8.415975474762904	12.726426621095957
3	2.5392159227475672	5.7596077625587005
4	1.16784213986125	3.531963072500895
5	0.6007540736255342	2.271113033256323
6	0.35902954697689937	1.6287463757855916
7	0.2229562163793938	1.18002075646771
8	0.1529435107683775	0.9251093341720513
9	0.11046626738726206	0.7516994109616066
>10	0.37054111811176144	4.456260531248582
>50	0.01106002305541747	0.5619081766532696
>100	0.004694658641217074	0.6642797282650493
>500	2.284369942671866E-4	0.12318272075778906
>1k	3.1981179107583215E-4	0.36291577725089375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.0749408793994084E-5
2	0.0	0.0	0.0	0.0	4.0749408793994084E-5
3	0.0	0.0	0.0	0.0	4.0749408793994084E-5
4	0.0	0.0	0.0	0.0	5.433254505865877E-5
5	0.0	0.0	0.0	1.3583136264664693E-5	5.433254505865877E-5
6	0.0	0.0	0.0	1.3583136264664693E-5	6.791568132332347E-5
7	0.0	0.0	0.0	1.3583136264664693E-5	6.791568132332347E-5
8	0.0	0.0	0.0	2.7166272529329386E-5	8.149881758798817E-5
9	0.0	0.0	0.0	2.7166272529329386E-5	8.149881758798817E-5
10-11	0.0	0.0	0.0	6.791568132332347E-5	8.149881758798817E-5
12-13	0.0	0.0	0.0	1.1545665824964988E-4	8.829038572032051E-5
14-15	0.0	0.0	0.0	2.512880208962968E-4	1.5620606704364398E-4
16-17	0.0	0.0	0.0	5.569085868512524E-4	2.1053861210230273E-4
18-19	0.0	0.0	0.0	0.0011070256055701725	2.444964527639645E-4
20-21	0.0	0.0	0.0	0.00243138139137498	2.580795890286292E-4
22-23	0.0	0.0	0.0	0.005351755688277889	3.056205659549556E-4
24-25	0.0	0.0	0.0	0.009413113431412632	3.4636997474894965E-4
26-27	0.0	0.0	0.0	0.01401100505700163	3.6674467914594674E-4
28-29	0.0	0.0	0.0	0.03826369485756044	3.6674467914594674E-4
30-31	0.0	0.0	0.0	0.050264395747391694	3.7353624727827907E-4
32-33	0.0	0.0	0.0	0.05590818886535988	4.074940879399408E-4
34-35	0.0	0.0	0.0	0.06278804738341255	4.482434967339349E-4
36-37	0.0	0.0	0.0	0.07306368996763138	4.482434967339349E-4
38-39	0.0	0.0	0.0	0.09990396722660883	4.550350648662672E-4
40-41	0.0	0.0	0.0	0.12941333076159287	4.754097692632643E-4
42-43	0.0	0.0	0.0	0.14572667741545514	4.889929055279291E-4
44-45	0.0	0.0	0.0	0.16394845471450287	5.025760417925937E-4
46-47	0.0	0.0	0.0	0.18221098142234454	5.161591780572584E-4
48-49	0.0	0.0	0.0	0.20094891789944946	5.433254505865878E-4
50-51	0.0	0.0	0.0	0.2199177676930537	5.433254505865878E-4
52-53	0.0	0.0	0.0	0.26378450625978833	5.976579956452465E-4
54-55	0.0	0.0	0.0	0.3135938669423138	6.6557367696857E-4
56-57	0.0	0.0	0.0	0.3600006519905407	6.85948381365567E-4
58-59	0.0	0.0	0.0	0.3884437393287486	6.927399494978994E-4
60-61	0.0	0.0	0.0	0.4122346024963088	6.927399494978994E-4
62-63	0.0	0.0	0.0	0.4305922111580031	6.927399494978994E-4
64-65	0.0	0.0	0.0	0.43641937661554425	6.927399494978994E-4
66-67	0.0	0.0	0.0	0.4426812024335547	6.995315176302317E-4
68-69	0.0	0.0	0.0	0.44924185724938775	7.063230857625641E-4
70-71	0.0	0.0	0.0	0.45444419843875433	7.199062220272287E-4
72-73	0.0	0.0	0.0	0.45896059124675537	7.470724945565581E-4
74-75	0.0	0.0	0.0	0.46230204276786285	7.470724945565581E-4
76-77	0.0	0.0	0.0	0.4655484123351177	7.470724945565581E-4
78-79	0.0	0.0	0.0	0.4677149225693317	7.470724945565581E-4
80-81	0.0	0.0	0.0	0.46831937213310926	7.674471989535551E-4
82-83	0.0	0.0	0.0	0.46874724092544623	7.878219033505522E-4
84-85	0.0	0.0	0.0	0.4690664446276659	7.878219033505522E-4
86-87	0.0	0.0	0.0	0.4692566085353712	7.878219033505522E-4
88	0.0	0.0	0.0	0.4692837748079005	7.878219033505522E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21670	0.0	38.054832	1
GTATCAA	35485	0.0	31.69959	1
CGCTACG	315	1.8189894E-12	28.243824	94
ATCAACG	42520	0.0	25.839266	3
AACGCAG	43535	0.0	25.393404	6
CAACGCA	43590	0.0	25.195465	5
TCAACGC	43935	0.0	25.063774	4
TATCAAC	44790	0.0	24.984299	2
GTACTAG	2420	0.0	23.335281	1
ACGCAGA	47840	0.0	22.917337	7
CGCAGAG	48095	0.0	22.79583	8
GTACATG	31930	0.0	21.71736	1
TACATGG	31790	0.0	21.49952	2
GCAGAGT	52610	0.0	20.76055	9
ACATGGG	32075	0.0	20.71271	3
GAGTACT	33790	0.0	19.589243	12-13
CATGGGG	21785	0.0	19.002956	4
TAACGCC	965	0.0	18.932543	4
CAGAGTA	50240	0.0	18.747564	10-11
AGAGTAC	48445	0.0	18.71355	10-11
>>END_MODULE