FastQCFastQC Report
Fri 27 May 2016
SRR2138908_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138908_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8734686
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145400.053.205161
GTATCAA278200.040.384821
ATCAACG354050.031.2774013
TCAACGC359300.030.8203814
TATCAAC365100.030.5634482
CAACGCA362750.030.5038745
AACGCAG373300.029.7137016
ACGCAGA412650.026.808277
CGCAGAG416800.026.5413468
GCAGAGT466250.023.5353479
GTGGTAT64100.022.4964621
TACATGG287950.022.0052762
TGGTATC64700.021.2389982
ACATGGG291750.021.1660023
GAGTACT323750.020.55553812-13
GTACATG314400.020.4642731
CAGAGTA463700.020.08807410-11
AGAGTAC447700.019.98170510-11
CATGGGG190300.019.4118844
AGTACTT351350.018.69334212-13
Produced by FastQC (version 0.11.5)