##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138908_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8734686
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.699910105526406	33.0	33.0	33.0	33.0	33.0
2	32.702788972608744	33.0	33.0	33.0	33.0	33.0
3	32.708413444971	33.0	33.0	33.0	33.0	33.0
4	36.64222629182091	37.0	37.0	37.0	37.0	37.0
5	36.65465890817369	37.0	37.0	37.0	37.0	37.0
6	36.601140098224484	37.0	37.0	37.0	37.0	37.0
7	36.57836423656214	37.0	37.0	37.0	37.0	37.0
8	36.58069322698034	37.0	37.0	37.0	37.0	37.0
9	36.61622226603223	37.0	37.0	37.0	37.0	37.0
10-11	36.60009312298118	37.0	37.0	37.0	37.0	37.0
12-13	36.59149304279512	37.0	37.0	37.0	37.0	37.0
14-15	38.69721401547806	40.0	37.0	40.0	37.0	40.0
16-17	38.6375345948326	40.0	37.0	40.0	37.0	40.0
18-19	38.675376023820434	40.0	37.0	40.0	37.0	40.0
20-21	38.692508918964045	40.0	37.0	40.0	37.0	40.0
22-23	38.69161010279957	40.0	37.0	40.0	37.0	40.0
24-25	38.69231898939876	40.0	37.0	40.0	37.0	40.0
26-27	38.61076847818589	40.0	37.0	40.0	37.0	40.0
28-29	38.50031208032884	40.0	37.0	40.0	37.0	40.0
30-31	38.35903182696889	40.0	37.0	40.0	37.0	40.0
32-33	38.247892674800894	40.0	37.0	40.0	37.0	40.0
34-35	38.225495748609646	40.0	37.0	40.0	37.0	40.0
36-37	38.218343440149106	40.0	37.0	40.0	37.0	40.0
38-39	38.17437363809702	40.0	37.0	40.0	37.0	40.0
40-41	38.15549743328599	40.0	37.0	40.0	37.0	40.0
42-43	38.106045558221766	40.0	37.0	40.0	37.0	40.0
44-45	38.00449004577328	40.0	37.0	40.0	33.0	40.0
46-47	37.90671298833343	40.0	37.0	40.0	33.0	40.0
48-49	37.83953462517793	40.0	37.0	40.0	33.0	40.0
50-51	37.82633602420562	40.0	37.0	40.0	33.0	40.0
52-53	37.770514044477096	40.0	37.0	40.0	33.0	40.0
54-55	37.66905676832849	40.0	37.0	40.0	33.0	40.0
56-57	37.50336966007066	40.0	37.0	40.0	33.0	40.0
58-59	37.32040676920269	38.5	37.0	40.0	33.0	40.0
60-61	37.16606979738033	37.0	37.0	40.0	33.0	40.0
62-63	37.03463388157415	37.0	37.0	40.0	33.0	40.0
64-65	36.86677179099035	37.0	37.0	40.0	33.0	40.0
66-67	36.66119668853713	37.0	37.0	40.0	33.0	40.0
68-69	36.45610860245378	37.0	37.0	40.0	33.0	40.0
70-71	36.24524960323267	37.0	37.0	40.0	33.0	40.0
72-73	35.96197712887286	37.0	37.0	37.0	33.0	40.0
74-75	35.6061837970473	37.0	33.0	37.0	33.0	40.0
76-77	33.50892562074063	35.0	33.0	37.0	27.0	37.0
78-79	35.136534642024785	37.0	33.0	37.0	33.0	37.0
80-81	35.33657171442702	37.0	33.0	37.0	33.0	37.0
82-83	35.128234087710965	37.0	33.0	37.0	33.0	37.0
84-85	34.97612046922183	37.0	33.0	37.0	33.0	37.0
86-87	34.87499701111638	37.0	33.0	37.0	33.0	37.0
88-89	34.76824806362859	37.0	33.0	37.0	33.0	37.0
90-91	34.69561547022549	37.0	33.0	37.0	33.0	37.0
92-93	34.657879101042376	37.0	33.0	37.0	33.0	37.0
94-95	34.60979497815123	37.0	33.0	37.0	33.0	37.0
96-97	34.619463182212606	37.0	33.0	37.0	33.0	37.0
98-99	34.64562020208486	37.0	33.0	37.0	33.0	37.0
100	34.59337845652628	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	14.0
20	45.0
21	197.0
22	755.0
23	2254.0
24	5672.0
25	11773.0
26	21170.0
27	34956.0
28	53529.0
29	77135.0
30	106200.0
31	145296.0
32	196306.0
33	269628.0
34	380617.0
35	576302.0
36	1054457.0
37	2644123.0
38	3058779.0
39	95473.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.187582472913164	21.00018249081879	14.782672210540825	23.029562825727222
2	14.103198924635777	20.905946960620312	43.51768710138806	21.47316701335584
3	17.31995355981681	27.323219851174805	31.274653888174687	24.082172700833702
4	11.732304973527382	18.031146168276685	40.559134008938614	29.677414849257318
5	12.308891241196307	38.41983558424425	36.36682532148265	12.904447853076803
6	28.260798384738727	39.39921824322019	19.072534490650263	13.267448881390814
7	25.205073198967888	33.61896466570178	24.243241256754967	16.93272087857537
8	23.5849348219272	37.3375986268997	21.590793303846297	17.486673247326806
9	25.370414002289266	17.055873559736433	22.222458826796977	35.35125361117733
10-11	23.60226029967317	27.46969337465901	29.49258888345398	19.435457442213842
12-13	24.42604015998595	24.93195284598141	29.81251925660323	20.82948773742941
14-15	21.610771125601996	26.1455763836273	28.042026925753255	24.201625565017448
16-17	20.3835856702331	29.25497599512747	28.833232108541978	21.52820622609745
18-19	20.300022267545998	28.24954191250171	31.05158915290019	20.398846667052105
20-21	21.454535504404767	27.48458876987051	30.92977086100987	20.131104864714853
22-23	21.029131327125597	27.1421300762275	30.940360499099757	20.888378097547154
24-25	20.58305424104332	27.33076918276017	31.336116440446883	20.75006013574962
26-27	20.517627428691085	27.687331827244687	31.079771398172657	20.715269345891567
28-29	20.412080446296795	27.984440175376402	30.994201914845505	20.609277463481302
30-31	20.998004120307375	27.64080179742226	30.80049415224294	20.560699930027425
32-33	20.721644923695514	27.948599039752796	30.652345513412733	20.677410523138953
34-35	20.685453878011145	27.93697458211115	30.76501127395423	20.61256026592347
36-37	20.89366218163397	27.660862438517725	30.80176480066586	20.643710579182446
38-39	20.68483976580638	28.015841336045305	30.752638458131425	20.54668044001689
40-41	21.001637796525532	27.92354429077798	30.49028458725332	20.584533325443168
42-43	21.026902360989403	27.981922738075514	30.182128236684306	20.809046664250776
44-45	20.95371534805598	28.457341911732286	29.818182078162188	20.770760662049543
46-47	21.09542872958507	28.072561574964283	29.48874829062496	21.343261404825686
48-49	21.265396381830513	28.17912655131017	29.431719008630218	21.123758058229104
50-51	20.985461144886415	28.38867274654856	29.191554773367645	21.434311335197375
52-53	21.197525115830434	28.444633089028038	28.85872056837952	21.499121226762007
54-55	21.332649840311177	28.268444991238862	28.93070002112615	21.46820514732381
56-57	21.387364578946215	28.33877271137692	28.777812768721745	21.496049940955128
58-59	21.169769546200126	28.364951340842104	29.150229379984317	21.315049732973453
60-61	21.22889906718352	28.5108616000374	28.807836044646724	21.452403288132356
62-63	21.367794901228763	28.4281222895402	28.7090748596166	21.495007949614433
64-65	21.16455661162509	28.51008089500104	28.74097016568354	21.584392327690335
66-67	21.470085373943206	28.600418122586813	28.27331145907936	21.656185044390618
68-69	21.284550953178623	28.480538880109208	28.66507409793154	21.569836068780624
70-71	21.475361127034482	27.825541219520083	28.728714788926133	21.970382864519298
72-73	21.59336707133504	28.071728695720566	28.63192479398356	21.702979438960835
74-75	21.211766076636902	28.42742802665887	28.58095173538006	21.779854161324167
76-77	21.419773177190006	28.275759984566623	28.39454227121742	21.90992456702595
78-79	21.52284605814199	28.386334855200452	28.26148204307884	21.829337043578718
80-81	21.48048359477609	28.13156429820326	28.396383459605275	21.99156864741538
82-83	21.765453494761562	27.94730558571834	28.51584751914555	21.771393400374546
84-85	22.10123565381031	27.99010214250303	28.22122672880688	21.687435474879774
86-87	21.3974464596056	28.247939541581868	28.383298653992185	21.971315344820347
88-89	21.80704229180677	28.080583689269723	28.354341812527473	21.758032206396035
90-91	21.94486719435431	27.930651825501606	28.514530461333692	21.609950518810393
92-93	22.04728777008647	27.93979882132759	28.23456190416055	21.778351504425398
94-95	21.6987555267636	27.998177584536826	28.33531826963782	21.96774861906175
96-97	21.6301662346786	28.14298686866539	28.58611132769583	21.64073556896018
98-99	22.781114324685966	30.092580469143638	28.684787885151668	18.441517321018726
100	22.135903499254226	30.780976687861255	22.447452498260823	24.635667314623692
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2591.0
1	2371.0
2	3678.0
3	5527.0
4	7674.0
5	13173.0
6	17777.5
7	20770.0
8	22451.5
9	21276.5
10	18402.0
11	15877.0
12	17368.5
13	23505.5
14	37645.5
15	57761.5
16	74276.0
17	83012.0
18	83389.0
19	76738.5
20	67111.0
21	57226.5
22	50940.0
23	51043.0
24	56620.5
25	69049.5
26	90216.5
27	113155.5
28	126637.5
29	141265.0
30	155478.5
31	168203.5
32	192310.0
33	220907.5
34	253617.5
35	283778.0
36	315360.0
37	343098.5
38	344139.5
39	341071.5
40	354066.0
41	358917.5
42	356865.0
43	365667.0
44	372169.5
45	376820.5
46	380656.0
47	381682.0
48	379966.0
49	367404.5
50	346944.5
51	319526.5
52	286910.0
53	255578.0
54	225733.5
55	197331.5
56	170216.5
57	145341.5
58	123957.5
59	107241.5
60	87686.0
61	65088.0
62	48675.5
63	36493.5
64	27035.5
65	19530.0
66	14386.0
67	11335.5
68	9036.0
69	7092.0
70	5584.5
71	4233.5
72	3201.5
73	2423.0
74	1781.0
75	1311.5
76	967.0
77	657.0
78	426.0
79	284.0
80	212.0
81	152.5
82	96.5
83	63.5
84	38.5
85	26.0
86	23.0
87	18.0
88	13.0
89	9.5
90	6.5
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001797431527590116
3	1.0303747610389201E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	4.0070129595957996E-5
12-13	0.007659119057055973
14-15	0.0
16-17	1.1448608455988E-5
18-19	1.1448608455988E-5
20-21	1.144890533867608E-5
22-23	0.0
24-25	2.920175457883512E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	7.386836691641292E-4
36-37	0.02642908727051501
38-39	0.1252508775251606
40-41	0.14766353956831832
42-43	0.032866608983779924
44-45	0.002567781844590813
46-47	1.2036261645459276E-5
48-49	6.0791585471905314E-6
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.007551883650517784
64-65	0.07884333467496255
66-67	0.19033426480216242
68-69	0.1981259458124648
70-71	0.07446921027381864
72-73	0.0023028975568815694
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	122.0
20-21	578.0
22-23	1195.0
24-25	2224.0
26-27	3957.0
28-29	8988.0
30-31	20606.0
32-33	25476.0
34-35	32600.0
36-37	44869.0
38-39	49898.0
40-41	60150.0
42-43	71289.0
44-45	82527.0
46-47	86749.0
48-49	70717.0
50-51	58369.0
52-53	53895.0
54-55	54372.0
56-57	55990.0
58-59	62492.0
60-61	64376.0
62-63	69096.0
64-65	72404.0
66-67	81616.0
68-69	77671.0
70-71	77979.0
72-73	79081.0
74-75	75530.0
76-77	69684.0
78-79	74582.0
80-81	81147.0
82-83	76072.0
84-85	82448.0
86-87	93378.0
88-89	102564.0
90-91	104535.0
92-93	102543.0
94-95	111366.0
96-97	275987.0
98-99	526716.0
100-101	5588848.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.0764377235304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.38210142512732	67.4440676234664
2	8.629183858340701	13.474718722412632
3	2.4925410800598273	5.838261852318969
4	1.052855542962803	3.2881284092803624
5	0.5231646976680411	2.042341796831421
6	0.30708385598662	1.438560813470455
7	0.17623310857739696	0.9631757328667112
8	0.10768907542475512	0.6726383512724368
9	0.07726825750031331	0.5429547265256032
>10	0.2415233652268848	2.8611918490972426
>50	0.006129484127231149	0.32565569516152104
>100	0.003648827152758328	0.5521800781872455
>500	2.56631936718576E-4	0.14917477480443092
>1k	3.2078990868095815E-4	0.4069495743045536
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.1448608455988E-5	0.0
3	0.0	0.0	0.0	1.1448608455988E-5	0.0
4	0.0	0.0	0.0	3.4345825367964E-5	0.0
5	0.0	0.0	0.0	4.5794433823952E-5	0.0
6	0.0	0.0	0.0	4.5794433823952E-5	0.0
7	0.0	0.0	0.0	4.5794433823952E-5	0.0
8	0.0	0.0	0.0	4.5794433823952E-5	0.0
9	0.0	0.0	0.0	6.8691650735928E-5	0.0
10-11	0.0	0.0	0.0	1.0876178033188599E-4	0.0
12-13	0.0	0.0	0.0	1.31658997243862E-4	0.0
14-15	0.0	0.0	0.0	2.23247864891766E-4	0.0
16-17	0.0	0.0	0.0	2.69042298715718E-4	0.0
18-19	0.0	0.0	0.0	3.3200964522365204E-4	0.0
20-21	0.0	0.0	0.0	4.92290163607484E-4	5.724304227994E-6
22-23	0.0	0.0	0.0	8.586456341991E-4	1.1448608455988E-5
24-25	0.0	0.0	0.0	0.00194626343751796	1.1448608455988E-5
26-27	0.0	0.0	0.0	0.00417874208643562	1.1448608455988E-5
28-29	0.0	0.0	0.0	0.01199241735764743	1.1448608455988E-5
30-31	0.0	0.0	0.0	0.01485456947164443	1.1448608455988E-5
32-33	0.0	0.0	0.0	0.020424317485482592	1.1448608455988E-5
34-35	0.0	0.0	0.0	0.02753962764087913	1.1448608455988E-5
36-37	0.0	0.0	0.0	0.03895389027149917	1.1448608455988E-5
38-39	0.0	0.0	0.0	0.06753534128187322	1.1448608455988E-5
40-41	0.0	0.0	0.0	0.09778256482259351	1.1448608455988E-5
42-43	0.0	0.0	0.0	0.11719368045972116	1.1448608455988E-5
44-45	0.0	0.0	0.0	0.13876858309503054	1.1448608455988E-5
46-47	0.0	0.0	0.0	0.15890668536911345	1.1448608455988E-5
48-49	0.0	0.0	0.0	0.18085939208347043	1.1448608455988E-5
50-51	0.0	0.0	0.0	0.20069983053769763	1.1448608455988E-5
52-53	0.0	0.0	0.0	0.2668727874133083	1.1448608455988E-5
54-55	0.0	0.0	0.0	0.34966339946278546	1.1448608455988E-5
56-57	0.0	0.0	0.0	0.41870423275662116	1.1448608455988E-5
58-59	0.0	2.2897216911976E-5	0.0	0.44359923184416705	1.1448608455988E-5
60-61	0.0	2.2897216911976E-5	0.0	0.4614189909059123	1.1448608455988E-5
62-63	0.0	2.2897216911976E-5	0.0	0.4755751952617415	1.1448608455988E-5
64-65	0.0	2.2897216911976E-5	0.0	0.48134529392355946	1.1448608455988E-5
66-67	0.0	2.2897216911976E-5	0.0	0.4867833829401538	1.1448608455988E-5
68-69	0.0	2.2897216911976E-5	0.0	0.49237602817090387	1.1448608455988E-5
70-71	0.0	2.2897216911976E-5	0.0	0.497081406246315	1.1448608455988E-5
72-73	0.0	2.2897216911976E-5	0.0	0.500910965774843	1.1448608455988E-5
74-75	0.0	2.2897216911976E-5	0.0	0.5042081650101675	1.1448608455988E-5
76-77	0.0	2.2897216911976E-5	0.0	0.507516812853948	1.1448608455988E-5
78-79	0.0	2.2897216911976E-5	0.0	0.509503146421062	1.1448608455988E-5
80-81	0.0	2.2897216911976E-5	0.0	0.5102015115368772	1.1448608455988E-5
82-83	0.0	2.2897216911976E-5	0.0	0.5106995260047127	1.1448608455988E-5
84-85	0.0	2.2897216911976E-5	0.0	0.5109571196949725	1.1448608455988E-5
86-87	0.0	2.2897216911976E-5	0.0	0.5110773300837603	1.1448608455988E-5
88	0.0	2.2897216911976E-5	0.0	0.5111002273006723	1.1448608455988E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14540	0.0	53.20516	1
GTATCAA	27820	0.0	40.38482	1
ATCAACG	35405	0.0	31.277401	3
TCAACGC	35930	0.0	30.820381	4
TATCAAC	36510	0.0	30.563448	2
CAACGCA	36275	0.0	30.503874	5
AACGCAG	37330	0.0	29.713701	6
ACGCAGA	41265	0.0	26.80827	7
CGCAGAG	41680	0.0	26.541346	8
GCAGAGT	46625	0.0	23.535347	9
GTGGTAT	6410	0.0	22.496462	1
TACATGG	28795	0.0	22.005276	2
TGGTATC	6470	0.0	21.238998	2
ACATGGG	29175	0.0	21.166002	3
GAGTACT	32375	0.0	20.555538	12-13
GTACATG	31440	0.0	20.464273	1
CAGAGTA	46370	0.0	20.088074	10-11
AGAGTAC	44770	0.0	19.981705	10-11
CATGGGG	19030	0.0	19.411884	4
AGTACTT	35135	0.0	18.693342	12-13
>>END_MODULE