FastQCFastQC Report
Fri 27 May 2016
SRR2138907_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138907_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6513405
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113950.056.4767271
GTATCAA214100.041.4193421
ATCAACG274950.031.8251653
TATCAAC280550.031.407292
TCAACGC280450.031.2786084
CAACGCA287850.030.4291535
AACGCAG297100.029.6762566
GTGGTAT42350.027.1247791
ACCGAGG24900.026.95694490-91
CGCTACG1457.718621E-526.7051794
ACGCAGA328400.026.6887767
AGACCGA24450.026.67762488-89
CGCAGAG332150.026.4267648
GACCGAG24550.026.29858490-91
GAGACCG24650.025.669688-89
GCAGAGT364200.024.0414359
TACATGG219150.023.726962
CCCACGA27000.023.06292582-83
ACATGGG220450.023.0341973
TGGTATC47050.022.9354742
Produced by FastQC (version 0.11.5)