##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138907_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6513405
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69382281617679	33.0	33.0	33.0	33.0	33.0
2	32.69469609213614	33.0	33.0	33.0	33.0	33.0
3	32.70150267026233	33.0	33.0	33.0	33.0	33.0
4	36.635132315586084	37.0	37.0	37.0	37.0	37.0
5	36.64720910184458	37.0	37.0	37.0	37.0	37.0
6	36.59611508880532	37.0	37.0	37.0	37.0	37.0
7	36.57125113515895	37.0	37.0	37.0	37.0	37.0
8	36.57376441354407	37.0	37.0	37.0	37.0	37.0
9	36.608568482997754	37.0	37.0	37.0	37.0	37.0
10-11	36.59169627867452	37.0	37.0	37.0	37.0	37.0
12-13	36.57953436029235	37.0	37.0	37.0	37.0	37.0
14-15	38.67045761778977	40.0	37.0	40.0	37.0	40.0
16-17	38.61406645218592	40.0	37.0	40.0	37.0	40.0
18-19	38.65881102127075	40.0	37.0	40.0	37.0	40.0
20-21	38.67489879422092	40.0	37.0	40.0	37.0	40.0
22-23	38.67115192151197	40.0	37.0	40.0	37.0	40.0
24-25	38.66704132791362	40.0	37.0	40.0	37.0	40.0
26-27	38.58431661661941	40.0	37.0	40.0	37.0	40.0
28-29	38.47054098557098	40.0	37.0	40.0	37.0	40.0
30-31	38.325850638662175	40.0	37.0	40.0	37.0	40.0
32-33	38.20721407516821	40.0	37.0	40.0	37.0	40.0
34-35	38.17847501883328	40.0	37.0	40.0	37.0	40.0
36-37	38.1729921166915	40.0	37.0	40.0	37.0	40.0
38-39	38.12402021438719	40.0	37.0	40.0	37.0	40.0
40-41	38.10144238736228	40.0	37.0	40.0	37.0	40.0
42-43	38.04762410586274	40.0	37.0	40.0	35.0	40.0
44-45	37.93890796022586	40.0	37.0	40.0	33.0	40.0
46-47	37.835221693530976	40.0	37.0	40.0	33.0	40.0
48-49	37.756078391065905	40.0	37.0	40.0	33.0	40.0
50-51	37.74339715263535	40.0	37.0	40.0	33.0	40.0
52-53	37.68866983027584	40.0	37.0	40.0	33.0	40.0
54-55	37.58340382203074	40.0	37.0	40.0	33.0	40.0
56-57	37.41843687754087	40.0	37.0	40.0	33.0	40.0
58-59	37.22723661387032	37.0	37.0	40.0	33.0	40.0
60-61	37.07069671073164	37.0	37.0	40.0	33.0	40.0
62-63	36.93556823497558	37.0	37.0	40.0	33.0	40.0
64-65	36.76397001978512	37.0	37.0	40.0	33.0	40.0
66-67	36.55768158503159	37.0	37.0	40.0	33.0	40.0
68-69	36.34771680762408	37.0	37.0	40.0	33.0	40.0
70-71	36.1429914320155	37.0	37.0	38.5	33.0	40.0
72-73	35.86360602190132	37.0	37.0	37.0	33.0	40.0
74-75	35.507052967295905	37.0	33.0	37.0	33.0	40.0
76-77	33.397237503275576	35.0	33.0	37.0	27.0	37.0
78-79	35.05083841278519	37.0	33.0	37.0	33.0	37.0
80-81	35.26509508342195	37.0	33.0	37.0	33.0	37.0
82-83	35.06479821763202	37.0	33.0	37.0	33.0	37.0
84-85	34.9136846697961	37.0	33.0	37.0	33.0	37.0
86-87	34.81399271147634	37.0	33.0	37.0	33.0	37.0
88-89	34.70521237910536	37.0	33.0	37.0	33.0	37.0
90-91	34.631016999485716	37.0	33.0	37.0	33.0	37.0
92-93	34.5952075605062	37.0	33.0	37.0	33.0	37.0
94-95	34.54375223318757	37.0	33.0	37.0	33.0	37.0
96-97	34.557918693358914	37.0	33.0	37.0	33.0	37.0
98-99	34.58635210006334	37.0	33.0	37.0	33.0	37.0
100	34.53219058435944	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	8.0
20	41.0
21	184.0
22	660.0
23	1840.0
24	4666.0
25	9399.0
26	17190.0
27	28002.0
28	43136.0
29	61732.0
30	84211.0
31	114331.0
32	154699.0
33	211309.0
34	297677.0
35	448679.0
36	829236.0
37	2036858.0
38	2112590.0
39	56955.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.50092002570084	20.962123497617604	14.755953299387956	22.781003177293595
2	14.305386115982339	21.014625147121997	43.00893770932163	21.67105102757403
3	17.24109342720352	27.412859536167943	31.422260475033593	23.92378656159494
4	11.848871673111068	17.92411188924994	40.48851560742806	29.738500830210928
5	12.239819265038793	38.6029119945712	36.329523498078196	12.827745242311817
6	28.423151945871627	39.378297526409	18.838426291624735	13.36012423609464
7	25.16659105337377	33.90361876775665	24.10565595107321	16.824134227796367
8	24.074888633518107	36.77076736361396	21.493888373285554	17.660455629582376
9	25.70736811237747	16.81283445448272	22.065663044137438	35.41413438900237
10-11	23.79611519538134	27.475082727486484	29.310459577700044	19.418342499432132
12-13	24.61904219393864	24.910677627843462	29.616350623360375	20.853929554857526
14-15	21.71245301036862	26.093694465490785	28.064829378796496	24.129023145344103
16-17	20.435947096794994	29.128059747551394	28.759527466816508	21.676465688837098
18-19	20.389542796739953	28.07417932709543	31.139511515098476	20.396766361066142
20-21	21.564171522222377	27.42506095465339	31.053368417747624	19.95739910537661
22-23	21.082117754557945	26.814942415871695	31.178581236217955	20.924358593352405
24-25	20.6215458838084	27.0285187066671	31.62409418231161	20.72584122721289
26-27	20.5359616450902	27.42606317844018	31.287476488446742	20.75049868802288
28-29	20.5460580426912	27.852839393160917	31.11433935101056	20.486763213137323
30-31	21.150393976652357	27.428048158839424	30.938595464117896	20.482962400390324
32-33	20.938283531155225	27.751192522040498	30.72575295087605	20.584770995928228
34-35	20.76371509771601	27.715932505580128	30.90300342802646	20.6173489686774
36-37	20.97512314420594	27.361979175133143	31.073002216804706	20.589895463856205
38-39	20.783223599983096	27.882636794833232	30.912130248121255	20.42200935706242
40-41	21.130568787320883	27.83052690026009	30.55609261911024	20.482811693308786
42-43	21.132772644076994	27.849996208037243	30.203694857238467	20.8135362906473
44-45	21.022529458748867	28.332649317101303	29.90228380717801	20.742537416971814
46-47	21.21295280346491	27.850306306888022	29.49259202461027	21.444148865036798
48-49	21.39580322258953	28.126181374891118	29.457192770027497	21.020822632491853
50-51	21.038552977235202	28.32022881262388	29.262499202554515	21.378719007586398
52-53	21.3760823129708	28.33969768025064	28.80830117379694	21.475918832981616
54-55	21.431095722051946	28.11948821442544	28.89919401752032	21.550222046002297
56-57	21.571532213097104	28.22754491212425	28.688600720100826	21.51232215467781
58-59	21.337013097180023	28.283556074323883	29.095278753682628	21.284152074813463
60-61	21.38537109449508	28.327837862573773	28.841406100471907	21.445384942459235
62-63	21.489241416567744	28.178695204849873	28.772135671902987	21.559927706679392
64-65	21.241735907239693	28.30020682288535	28.85398781463116	21.604069455243796
66-67	21.588567299707385	28.471921275369784	28.357734768511822	21.581776656411012
68-69	21.39703751467268	28.412455219664267	28.67386688319065	21.516640382472403
70-71	21.475993476814327	27.715860654172985	28.6897024614663	22.118443407546387
72-73	21.698178950587003	27.97332564303178	28.655343775923146	21.67315163045807
74-75	21.12395910116856	28.401605102089327	28.75231377244447	21.72212202429764
76-77	21.40065479152871	28.227435454275778	28.440370435757767	21.931539318437746
78-79	21.550794734521457	28.349558730999036	28.226474296229178	21.873172238250334
80-81	21.695181037307243	28.066742160116377	28.379577552133856	21.858499250442527
82-83	21.90868199564462	27.713534913121673	28.54575444497881	21.8320286462549
84-85	22.203040028446424	27.788647823353564	28.191021780211038	21.817290367988974
86-87	21.297115833874226	28.23111813253797	28.331763532297266	22.14000250129054
88-89	21.976596378048956	27.913618315828977	28.366087086153602	21.74369821996846
90-91	21.999452856105453	27.82294499962521	28.620438259394902	21.55716388487443
92-93	22.060191723538626	27.889003856171374	28.106969800192548	21.94383462009745
94-95	21.83253434576395	27.968875085444022	28.22329909401024	21.975291474781788
96-97	21.851600596067705	27.9628012128668	28.5435513919563	21.642046799109195
98-99	22.81679762852498	30.040876832235046	28.646408647186444	18.49591689205353
100	22.288927504396785	30.734591380589183	22.424503115954725	24.551977999059307
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2466.0
1	2139.0
2	3035.5
3	4521.0
4	6245.5
5	10687.5
6	14437.0
7	16919.5
8	18398.5
9	17483.5
10	14928.0
11	12649.5
12	13770.5
13	18904.0
14	30993.5
15	47511.0
16	60286.5
17	66508.5
18	66000.5
19	59609.5
20	50540.0
21	41299.5
22	35322.0
23	34545.0
24	37912.5
25	46305.0
26	62474.5
27	79993.5
28	87701.0
29	96718.0
30	104062.0
31	109901.0
32	128433.5
33	150367.0
34	174014.5
35	194100.0
36	219461.5
37	242246.0
38	238382.5
39	234127.0
40	247735.5
41	254724.0
42	253555.0
43	258818.5
44	263331.0
45	266340.0
46	266770.0
47	266400.5
48	263793.0
49	255290.5
50	242737.0
51	226859.0
52	208814.0
53	190066.0
54	171385.5
55	152639.5
56	133530.5
57	115948.5
58	100610.0
59	88413.0
60	72740.5
61	52955.0
62	38616.0
63	27990.0
64	19809.0
65	13753.0
66	9710.0
67	7226.5
68	5318.5
69	3945.0
70	3036.0
71	2081.0
72	1493.0
73	1042.0
74	660.0
75	416.0
76	280.5
77	205.0
78	146.5
79	98.0
80	70.0
81	49.5
82	35.5
83	23.5
84	18.5
85	13.5
86	7.0
87	4.5
88	3.0
89	1.0
90	0.0
91	0.5
92	1.0
93	1.0
94	2.0
95	2.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0015813541457962463
3	1.9958838733350682E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	2.3029429307712327E-5
12-13	0.007354064425596135
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	7.67721782539427E-6
24-25	2.533856741114705E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	6.948810506477952E-4
36-37	0.026590249364844377
38-39	0.12483216633736806
40-41	0.14599770428298062
42-43	0.03291278284496032
44-45	0.0024839351884713336
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.00717020081007628
64-65	0.0749983982130183
66-67	0.17850385503379954
68-69	0.18708628310451775
70-71	0.07086246371609368
72-73	0.0023830878193671053
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	96.0
20-21	360.0
22-23	805.0
24-25	1688.0
26-27	2753.0
28-29	5850.0
30-31	10611.0
32-33	11789.0
34-35	14740.0
36-37	16478.0
38-39	17281.0
40-41	25472.0
42-43	34464.0
44-45	41728.0
46-47	43509.0
48-49	33249.0
50-51	27743.0
52-53	31979.0
54-55	28700.0
56-57	25662.0
58-59	20025.0
60-61	36532.0
62-63	30812.0
64-65	45816.0
66-67	60273.0
68-69	58080.0
70-71	60502.0
72-73	59576.0
74-75	57868.0
76-77	53974.0
78-79	59128.0
80-81	64927.0
82-83	59300.0
84-85	65307.0
86-87	71160.0
88-89	78386.0
90-91	80960.0
92-93	78850.0
94-95	86023.0
96-97	223579.0
98-99	409739.0
100-101	4377631.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.34525028429418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.2964764430584	65.11980842413695
2	9.231652039868209	14.095855710425065
3	2.773971537779303	6.353386539998078
4	1.126798934296838	3.4410298663587224
5	0.5762532983710557	2.199710114564415
6	0.3281859711661657	1.5033264065085044
7	0.19061451632491794	1.0186759069651876
8	0.11385731374081139	0.695397209139172
9	0.08697397583609721	0.5976044958084296
>10	0.2619312602764352	3.1222448085268146
>50	0.007950602918087862	0.42105814260366753
>100	0.004588357449472127	0.7531440035641196
>500	4.386758319149331E-4	0.2577118304940106
>1k	3.0707308225049574E-4	0.4210465409069081
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5352952871808216E-5	0.0	0.0	0.0
3	0.0	1.5352952871808216E-5	0.0	0.0	0.0
4	0.0	1.5352952871808216E-5	0.0	0.0	0.0
5	0.0	1.5352952871808216E-5	0.0	0.0	0.0
6	0.0	1.5352952871808216E-5	0.0	0.0	0.0
7	0.0	1.5352952871808216E-5	0.0	0.0	0.0
8	0.0	1.5352952871808216E-5	0.0	0.0	0.0
9	0.0	4.605885861542465E-5	0.0	0.0	0.0
10-11	0.0	4.605885861542465E-5	0.0	0.0	0.0
12-13	0.0	4.605885861542465E-5	0.0	0.0	0.0
14-15	0.0	8.44412407949452E-5	0.0	0.0	0.0
16-17	0.0	9.21177172308493E-5	0.0	0.0	0.0
18-19	0.0	9.979419366675341E-5	0.0	3.838238217952054E-5	0.0
20-21	0.0	1.0747067010265753E-4	0.0	9.21177172308493E-5	0.0
22-23	0.0	1.2282362297446573E-4	0.0	3.454414396156849E-4	0.0
24-25	0.0	1.2282362297446573E-4	0.0	0.001136118512513808	0.0
26-27	0.0	1.2282362297446573E-4	0.0	0.0026790902761305337	0.0
28-29	0.0	1.2282362297446573E-4	0.0	0.00762274110085278	0.0
30-31	0.0	1.5352952871808216E-4	0.0	0.008421094650186808	0.0
32-33	0.0	1.5352952871808216E-4	0.0	0.009856595743700874	0.0
34-35	0.0	2.1494134020531505E-4	0.0	0.011315126266522656	0.0
36-37	0.0	2.610001988207397E-4	0.0	0.013280304234114108	0.0
38-39	0.0	2.610001988207397E-4	0.0	0.017218336645732916	0.0
40-41	0.0	2.763531516925479E-4	0.0	0.021601604690634164	0.0
42-43	0.0	2.84029628128452E-4	0.0	0.026007902164843118	0.0
44-45	0.0	3.377649631797808E-4	0.0	0.03042187611548798	0.0
46-47	0.0	3.8382382179520544E-4	0.0	0.03478211473108152	0.0
48-49	0.0	3.8382382179520544E-4	0.0	0.040746736921779006	0.0
50-51	0.0	4.452356332824383E-4	0.0	0.046243094049886344	0.0
52-53	0.0	4.452356332824383E-4	0.0	0.06525772618162083	0.0
54-55	0.0	4.452356332824383E-4	0.0	0.09012183335751424	0.0
56-57	0.0	4.452356332824383E-4	0.0	0.1078161115422732	0.0
58-59	0.0	4.529121097183424E-4	0.0	0.20245938952053494	0.0
60-61	0.0	4.759415390260547E-4	0.0	0.2904471624288678	0.0
62-63	7.676476435904108E-6	5.527063033850958E-4	0.0	0.29393995920720417	0.0
64-65	1.5352952871808216E-5	6.448240206159452E-4	0.0	0.2971256969281044	0.0
66-67	1.5352952871808216E-5	6.448240206159452E-4	0.0	0.3022612596637243	0.0
68-69	1.5352952871808216E-5	6.525004970518492E-4	0.0	0.30843314671819116	0.0
70-71	1.5352952871808216E-5	6.601769734877534E-4	0.0	0.3133767975429134	0.0
72-73	1.5352952871808216E-5	6.601769734877534E-4	0.0	0.3176065360590966	0.0
74-75	1.5352952871808216E-5	6.755299263595616E-4	0.0	0.3212375094132792	0.0
76-77	1.5352952871808216E-5	6.832064027954656E-4	0.0	0.32500665934330814	0.0
78-79	1.5352952871808216E-5	6.908828792313698E-4	0.0	0.3272712198918999	0.0
80-81	1.5352952871808216E-5	6.908828792313698E-4	0.0	0.3281002793469775	0.0
82-83	1.5352952871808216E-5	6.908828792313698E-4	0.0	0.32872975041472163	0.0
84-85	1.5352952871808216E-5	6.908828792313698E-4	0.0	0.329021456519286	0.0
86-87	1.5352952871808216E-5	7.13912308539082E-4	0.0	0.32912125071295273	0.0
88	1.5352952871808216E-5	7.522946907186027E-4	0.0	0.32913660366582453	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11395	0.0	56.476727	1
GTATCAA	21410	0.0	41.419342	1
ATCAACG	27495	0.0	31.825165	3
TATCAAC	28055	0.0	31.40729	2
TCAACGC	28045	0.0	31.278608	4
CAACGCA	28785	0.0	30.429153	5
AACGCAG	29710	0.0	29.676256	6
GTGGTAT	4235	0.0	27.124779	1
ACCGAGG	2490	0.0	26.956944	90-91
CGCTACG	145	7.718621E-5	26.70517	94
ACGCAGA	32840	0.0	26.688776	7
AGACCGA	2445	0.0	26.677624	88-89
CGCAGAG	33215	0.0	26.426764	8
GACCGAG	2455	0.0	26.298584	90-91
GAGACCG	2465	0.0	25.6696	88-89
GCAGAGT	36420	0.0	24.041435	9
TACATGG	21915	0.0	23.72696	2
CCCACGA	2700	0.0	23.062925	82-83
ACATGGG	22045	0.0	23.034197	3
TGGTATC	4705	0.0	22.935474	2
>>END_MODULE