FastQCFastQC Report
Fri 27 May 2016
SRR2138906_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138906_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7862313
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101050.049.4391631
ATCTCGT48350.044.6879194
GTATCAA185000.038.9355851
TATCTCG63550.036.0931894
TCAACGC219650.032.1558724
ATCAACG220650.032.010143
CAACGCA224050.031.5450925
AACGCAG231200.030.5913626
TATCAAC232100.030.507112
ACGCAGA260200.027.166287
CGCAGAG263000.026.8438178
CGCTATC98200.024.00289394
GCAGAGT310000.022.6470329
GTGGTAT50400.022.547791
CTATCTC113450.021.2790794
GCTATCT108050.020.7531592-93
GTACATG209650.020.5145231
GAGTACT200850.020.43543812-13
TACATGG205650.020.0846522
TGGTATC51900.019.9591562
Produced by FastQC (version 0.11.5)